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The map label for this gene is gyaR [H]

Identifier: 50843685

GI number: 50843685

Start: 2435865

End: 2436830

Strand: Reverse

Name: gyaR [H]

Synonym: PPA2251

Alternate gene names: 50843685

Gene position: 2436830-2435865 (Counterclockwise)

Preceding gene: 50843688

Following gene: 50843684

Centisome position: 95.18

GC content: 60.14

Gene sequence:

>966_bases
ATGGCCCGCATTATTGTCACCGCACCGCAGCTCATGTCCGTCGTTAACGAGGAGCTCCCCGGTCACGAGGTCGTAGGTGG
CGACCACTTCATGGACCGCCAGGAACTGTCGCGCCAGATCGCTACCGCTGATGCCATACTGACGAGCTTGTCCGATCCCC
TCGACGCCGAGATGATTGGTCAAGGCAAGAACCTGAAGGTCATCGGTCAGTGCGCCGCTGGTTTTAATAACATCGACCTT
GACGCCGCTAAGCAGGCTGGGGTTGTCGTCACATCGACCCCAGGCGTCCTCCACGAGGCCACTGCTGACCTTGCCTTCAC
GTTGCTTCTTGAGGTCACTCGTCGTACTGGCGAGGCTGAACGTTGGGTGCGTGCAGGCAGGGCTTGGCGCTACGATCACA
CCTTCATGCTCGGCGCTGGTTTACAGGGGGCTACCCTCGGCATTGTCGGTCTGGGACAGATCGGTGAGGCTATGGCTCGA
CGCGGAGCAGCCTTCGGCATGAATGTCATCTACAACGCCCGCCATGAGAAGGATGTCGCGGCGATTGACGCCGTCAATCT
CAATACCCAGCCCACTCGTCGCGTCGAGCTCGACGAACTGTTCGCAACCTCCGACGTTGTCTCCTTGCACTGTCCACTCA
CTGATGAGACCCGTCATCTCGTCGACGCTGACGCCTTGGCTGCGATGAAGAAGACCGCCTACCTCGTTAATACCGCTCGT
GGCGCCTGTGTCGACGAGGCTGCCCTGGTGGAGGCTCTTAAAACGGGTGCGATTGCTGGTGCAGGTCTGGATGTTTTCGA
GGAGGAGCCGACGATTACTGCGGACTTGCTGACGATGGAGAACGTCGTGCTGCTGCCCCACCTTGGGTCGGCTGCGCTGC
CGACCCGCGAAGCGATGAGTCGTCTGGCTGCTCGCAATATCGCCAAGGTCCTTGACGGTAAGCCGGCTGAAACCCCGGTG
GAGTGA

Upstream 100 bases:

>100_bases
TCGTTGCATCCCGCGATATCTGCACAGCGGAATTGTTGCACACTGCATTAGTTTTGGCGACGTGCTGTGTGTCGGTGGCG
CGGTCACCTACAGTGATGCT

Downstream 100 bases:

>100_bases
TTCGTTCGCTCACAGCGTTGATGTGGTAGGCCCGGTTCTCGTTGGCGGAACTTCGTCGCAGAGAGCCGGGCTCAGCTGTG
TTCTTTGACGTGAAGGAAAG

Product: D-isomer specific 2-hydroxyacid dehydrogenase, putative D-3-phosphoglycerate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MARIIVTAPQLMSVVNEELPGHEVVGGDHFMDRQELSRQIATADAILTSLSDPLDAEMIGQGKNLKVIGQCAAGFNNIDL
DAAKQAGVVVTSTPGVLHEATADLAFTLLLEVTRRTGEAERWVRAGRAWRYDHTFMLGAGLQGATLGIVGLGQIGEAMAR
RGAAFGMNVIYNARHEKDVAAIDAVNLNTQPTRRVELDELFATSDVVSLHCPLTDETRHLVDADALAAMKKTAYLVNTAR
GACVDEAALVEALKTGAIAGAGLDVFEEEPTITADLLTMENVVLLPHLGSAALPTREAMSRLAARNIAKVLDGKPAETPV
E

Sequences:

>Translated_321_residues
MARIIVTAPQLMSVVNEELPGHEVVGGDHFMDRQELSRQIATADAILTSLSDPLDAEMIGQGKNLKVIGQCAAGFNNIDL
DAAKQAGVVVTSTPGVLHEATADLAFTLLLEVTRRTGEAERWVRAGRAWRYDHTFMLGAGLQGATLGIVGLGQIGEAMAR
RGAAFGMNVIYNARHEKDVAAIDAVNLNTQPTRRVELDELFATSDVVSLHCPLTDETRHLVDADALAAMKKTAYLVNTAR
GACVDEAALVEALKTGAIAGAGLDVFEEEPTITADLLTMENVVLLPHLGSAALPTREAMSRLAARNIAKVLDGKPAETPV
E
>Mature_320_residues
ARIIVTAPQLMSVVNEELPGHEVVGGDHFMDRQELSRQIATADAILTSLSDPLDAEMIGQGKNLKVIGQCAAGFNNIDLD
AAKQAGVVVTSTPGVLHEATADLAFTLLLEVTRRTGEAERWVRAGRAWRYDHTFMLGAGLQGATLGIVGLGQIGEAMARR
GAAFGMNVIYNARHEKDVAAIDAVNLNTQPTRRVELDELFATSDVVSLHCPLTDETRHLVDADALAAMKKTAYLVNTARG
ACVDEAALVEALKTGAIAGAGLDVFEEEPTITADLLTMENVVLLPHLGSAALPTREAMSRLAARNIAKVLDGKPAETPVE

Specific function: Unknown

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GyaR subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=293, Percent_Identity=39.9317406143345, Blast_Score=180, Evalue=1e-45,
Organism=Homo sapiens, GI23308577, Length=293, Percent_Identity=32.4232081911263, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI61743967, Length=267, Percent_Identity=35.2059925093633, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI4557497, Length=267, Percent_Identity=35.2059925093633, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI145580578, Length=275, Percent_Identity=34.9090909090909, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI4557499, Length=275, Percent_Identity=34.9090909090909, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI145580575, Length=275, Percent_Identity=34.9090909090909, Blast_Score=137, Evalue=9e-33,
Organism=Escherichia coli, GI87082289, Length=279, Percent_Identity=40.1433691756272, Blast_Score=173, Evalue=1e-44,
Organism=Escherichia coli, GI1787645, Length=268, Percent_Identity=35.8208955223881, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI1789279, Length=294, Percent_Identity=30.952380952381, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1788660, Length=248, Percent_Identity=26.2096774193548, Blast_Score=72, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17532191, Length=283, Percent_Identity=32.1554770318021, Blast_Score=143, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI25147481, Length=294, Percent_Identity=30.952380952381, Blast_Score=129, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6324055, Length=254, Percent_Identity=36.6141732283465, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6320925, Length=305, Percent_Identity=31.4754098360656, Blast_Score=142, Evalue=9e-35,
Organism=Saccharomyces cerevisiae, GI6322116, Length=302, Percent_Identity=30.794701986755, Blast_Score=136, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6324964, Length=254, Percent_Identity=31.8897637795276, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6321253, Length=290, Percent_Identity=28.2758620689655, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324980, Length=140, Percent_Identity=37.8571428571429, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6325144, Length=175, Percent_Identity=29.1428571428571, Blast_Score=79, Evalue=8e-16,
Organism=Drosophila melanogaster, GI28571528, Length=286, Percent_Identity=40.5594405594406, Blast_Score=182, Evalue=2e-46,
Organism=Drosophila melanogaster, GI28574284, Length=292, Percent_Identity=37.6712328767123, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24585514, Length=292, Percent_Identity=37.6712328767123, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI28574282, Length=292, Percent_Identity=37.6712328767123, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI45552429, Length=292, Percent_Identity=37.6712328767123, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI45551003, Length=292, Percent_Identity=37.6712328767123, Blast_Score=178, Evalue=4e-45,
Organism=Drosophila melanogaster, GI28574286, Length=292, Percent_Identity=36.986301369863, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24585516, Length=297, Percent_Identity=31.3131313131313, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI19921140, Length=278, Percent_Identity=33.4532374100719, Blast_Score=144, Evalue=8e-35,
Organism=Drosophila melanogaster, GI62472511, Length=267, Percent_Identity=35.2059925093633, Blast_Score=141, Evalue=6e-34,
Organism=Drosophila melanogaster, GI24646446, Length=267, Percent_Identity=35.2059925093633, Blast_Score=141, Evalue=7e-34,
Organism=Drosophila melanogaster, GI24646448, Length=267, Percent_Identity=35.2059925093633, Blast_Score=141, Evalue=7e-34,
Organism=Drosophila melanogaster, GI24646452, Length=267, Percent_Identity=35.2059925093633, Blast_Score=141, Evalue=7e-34,
Organism=Drosophila melanogaster, GI24646450, Length=267, Percent_Identity=35.2059925093633, Blast_Score=141, Evalue=7e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.26 [H]

Molecular weight: Translated: 34108; Mature: 33977

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00670 D_2_HYDROXYACID_DH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARIIVTAPQLMSVVNEELPGHEVVGGDHFMDRQELSRQIATADAILTSLSDPLDAEMIG
CCEEEEECHHHHHHHHHCCCCCEECCCCHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHC
QGKNLKVIGQCAAGFNNIDLDAAKQAGVVVTSTPGVLHEATADLAFTLLLEVTRRTGEAE
CCCCEEEEEEHHCCCCCCCCCHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHH
RWVRAGRAWRYDHTFMLGAGLQGATLGIVGLGQIGEAMARRGAAFGMNVIYNARHEKDVA
HHHHHCCCEECCCEEEEECCCCCCEEEEEECHHHHHHHHHCCCCCCCHHEECCCCCCCHH
AIDAVNLNTQPTRRVELDELFATSDVVSLHCPLTDETRHLVDADALAAMKKTAYLVNTAR
EEEEECCCCCCCCCEEHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
GACVDEAALVEALKTGAIAGAGLDVFEEEPTITADLLTMENVVLLPHLGSAALPTREAMS
CCCHHHHHHHHHHHHCCCCCCCCHHHCCCCCCEEHHHHHCCEEEEECCCCCCCCHHHHHH
RLAARNIAKVLDGKPAETPVE
HHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
ARIIVTAPQLMSVVNEELPGHEVVGGDHFMDRQELSRQIATADAILTSLSDPLDAEMIG
CEEEEECHHHHHHHHHCCCCCEECCCCHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHC
QGKNLKVIGQCAAGFNNIDLDAAKQAGVVVTSTPGVLHEATADLAFTLLLEVTRRTGEAE
CCCCEEEEEEHHCCCCCCCCCHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHH
RWVRAGRAWRYDHTFMLGAGLQGATLGIVGLGQIGEAMARRGAAFGMNVIYNARHEKDVA
HHHHHCCCEECCCEEEEECCCCCCEEEEEECHHHHHHHHHCCCCCCCHHEECCCCCCCHH
AIDAVNLNTQPTRRVELDELFATSDVVSLHCPLTDETRHLVDADALAAMKKTAYLVNTAR
EEEEECCCCCCCCCEEHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
GACVDEAALVEALKTGAIAGAGLDVFEEEPTITADLLTMENVVLLPHLGSAALPTREAMS
CCCHHHHHHHHHHHHCCCCCCCCHHHCCCCCCEEHHHHHCCEEEEECCCCCCCCHHHHHH
RLAARNIAKVLDGKPAETPVE
HHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA