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The map label for this gene is nrdF

Identifier: 50843560

GI number: 50843560

Start: 2297482

End: 2298468

Strand: Direct

Name: nrdF

Synonym: PPA2122

Alternate gene names: 50843560

Gene position: 2297482-2298468 (Clockwise)

Preceding gene: 50843559

Following gene: 50843561

Centisome position: 89.74

GC content: 55.22

Gene sequence:

>987_bases
ATGATCCTTGGTTCTGGAATCCAAGAGGGGCTGCTACTCAAGCCCGTCACCTACTCCTGGGCGTACGACCTCTACAACCA
GGCTGTGGCAAACACGTGGTTTCCCAACGAGATTCAGCTCGATGAGGACCTTGCCGACTTTGCCCGGATGACCCCAGACG
AGCACCATGCCTTGACCCACCTCATGAGCTACTTCAACCCCAATGAGCTGCTCGTCAACAAAGCGCTGGCTTTTGGGGTC
TACCCCTATGTCAACGCTGCCGAGTGCCACCTATACCTGGCCAAGCAGATGTGGGAGGAGGCCAACCACTGTATGGCCTT
CGAGTATGTCCTCGAAACCTTCCCGATTGACCGCGACGAGGCCTACCGATCCCACATCCACGTCCCCTCAATGGCTCGCA
AGGAAGCTTTCGAGGTGGCGTTCATCAAGCGGATGACCGAGCAGACTCTTGATATCACCACCGTCGAGGGTAAGCAGGAC
TTCGTGCGCAACCTGATTGCCTACAACATTATCCTCGAGGGGATTTGGTTCTACTCCGGCTTTATGGTCGCATTGTCGTT
CCGTCAGCGGAACCTCCTTCGCAACTTCGGTTCCCTCATCGACTGGGTGGTCCGTGACGAATCCTTACATCTGAAATTCG
GGATGAACCTCATTCTGACGGTGCTGGAGGAAAACCCCGAAATCGCCACCGACGAGTTCGCGGCCGAGATCCACGATATG
ATCGTTCAGGGAGTCGAGATGGAGGAGCAGTATAACCGTGACATGCTCCCGCGGGGGATCCTCGGAATGAACGCTGATTA
CGTTAATGACTATGTCAAATACCTGGCAGATCGTCGTCTCGAAGAACTCGGATTCGAGCCAGAATACCGCGTAGTTAACC
CTGCCAAGTGGATGGCCACGGCCAATGACACCTTGCAGGTTGTGAACTTCTTCGAGGCCACCAATACCTCTTATGAGGTC
AATGCCCACGCCACCGGCAGTCGGTGA

Upstream 100 bases:

>100_bases
CTTATCGACGGCGCCTCCTGCCCTGTTGACCCCCAGGAGCGCCAACAGTGCGAGTCCTGCCAGTGATTGACCATACTGCC
CGCTGAAAGCGAGAACACCG

Downstream 100 bases:

>100_bases
CGCGCGCCGCGACGAGTCGAACATTCGCTGCGGCGCGCGGTGAGCGTGCATGAAGTCGCCACAGCATGCACGCTGACGTG
TCGGCCCCAAGTATGGTCTT

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Ribonucleotide reductase small subunit [H]

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MILGSGIQEGLLLKPVTYSWAYDLYNQAVANTWFPNEIQLDEDLADFARMTPDEHHALTHLMSYFNPNELLVNKALAFGV
YPYVNAAECHLYLAKQMWEEANHCMAFEYVLETFPIDRDEAYRSHIHVPSMARKEAFEVAFIKRMTEQTLDITTVEGKQD
FVRNLIAYNIILEGIWFYSGFMVALSFRQRNLLRNFGSLIDWVVRDESLHLKFGMNLILTVLEENPEIATDEFAAEIHDM
IVQGVEMEEQYNRDMLPRGILGMNADYVNDYVKYLADRRLEELGFEPEYRVVNPAKWMATANDTLQVVNFFEATNTSYEV
NAHATGSR

Sequences:

>Translated_328_residues
MILGSGIQEGLLLKPVTYSWAYDLYNQAVANTWFPNEIQLDEDLADFARMTPDEHHALTHLMSYFNPNELLVNKALAFGV
YPYVNAAECHLYLAKQMWEEANHCMAFEYVLETFPIDRDEAYRSHIHVPSMARKEAFEVAFIKRMTEQTLDITTVEGKQD
FVRNLIAYNIILEGIWFYSGFMVALSFRQRNLLRNFGSLIDWVVRDESLHLKFGMNLILTVLEENPEIATDEFAAEIHDM
IVQGVEMEEQYNRDMLPRGILGMNADYVNDYVKYLADRRLEELGFEPEYRVVNPAKWMATANDTLQVVNFFEATNTSYEV
NAHATGSR
>Mature_328_residues
MILGSGIQEGLLLKPVTYSWAYDLYNQAVANTWFPNEIQLDEDLADFARMTPDEHHALTHLMSYFNPNELLVNKALAFGV
YPYVNAAECHLYLAKQMWEEANHCMAFEYVLETFPIDRDEAYRSHIHVPSMARKEAFEVAFIKRMTEQTLDITTVEGKQD
FVRNLIAYNIILEGIWFYSGFMVALSFRQRNLLRNFGSLIDWVVRDESLHLKFGMNLILTVLEENPEIATDEFAAEIHDM
IVQGVEMEEQYNRDMLPRGILGMNADYVNDYVKYLADRRLEELGFEPEYRVVNPAKWMATANDTLQVVNFFEATNTSYEV
NAHATGSR

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Homo sapiens, GI42544136, Length=309, Percent_Identity=24.5954692556634, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI289177074, Length=309, Percent_Identity=24.5954692556634, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI4557845, Length=309, Percent_Identity=22.6537216828479, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI260064013, Length=309, Percent_Identity=22.6537216828479, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI289177076, Length=288, Percent_Identity=23.9583333333333, Blast_Score=96, Evalue=4e-20,
Organism=Escherichia coli, GI1788567, Length=316, Percent_Identity=20.5696202531646, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1789031, Length=307, Percent_Identity=23.7785016286645, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17551968, Length=305, Percent_Identity=25.9016393442623, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI193206379, Length=283, Percent_Identity=23.321554770318, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6322434, Length=316, Percent_Identity=26.5822784810127, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321619, Length=319, Percent_Identity=22.5705329153605, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI18079279, Length=308, Percent_Identity=25.6493506493506, Blast_Score=112, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 38005; Mature: 38005

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILGSGIQEGLLLKPVTYSWAYDLYNQAVANTWFPNEIQLDEDLADFARMTPDEHHALTH
CCCCCCCCCCCEECCCHHHHHHHHHHHHHHCCCCCCCEECCHHHHHHHHCCCCHHHHHHH
LMSYFNPNELLVNKALAFGVYPYVNAAECHLYLAKQMWEEANHCMAFEYVLETFPIDRDE
HHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHH
AYRSHIHVPSMARKEAFEVAFIKRMTEQTLDITTVEGKQDFVRNLIAYNIILEGIWFYSG
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHH
FMVALSFRQRNLLRNFGSLIDWVVRDESLHLKFGMNLILTVLEENPEIATDEFAAEIHDM
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHH
IVQGVEMEEQYNRDMLPRGILGMNADYVNDYVKYLADRRLEELGFEPEYRVVNPAKWMAT
HHHCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHCC
ANDTLQVVNFFEATNTSYEVNAHATGSR
CCCHHHHHHHHHHCCCCEEEEECCCCCC
>Mature Secondary Structure
MILGSGIQEGLLLKPVTYSWAYDLYNQAVANTWFPNEIQLDEDLADFARMTPDEHHALTH
CCCCCCCCCCCEECCCHHHHHHHHHHHHHHCCCCCCCEECCHHHHHHHHCCCCHHHHHHH
LMSYFNPNELLVNKALAFGVYPYVNAAECHLYLAKQMWEEANHCMAFEYVLETFPIDRDE
HHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHH
AYRSHIHVPSMARKEAFEVAFIKRMTEQTLDITTVEGKQDFVRNLIAYNIILEGIWFYSG
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHH
FMVALSFRQRNLLRNFGSLIDWVVRDESLHLKFGMNLILTVLEENPEIATDEFAAEIHDM
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHH
IVQGVEMEEQYNRDMLPRGILGMNADYVNDYVKYLADRRLEELGFEPEYRVVNPAKWMAT
HHHCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHCC
ANDTLQVVNFFEATNTSYEVNAHATGSR
CCCHHHHHHHHHHCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10684935 [H]