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The map label for this gene is kgtP [H]

Identifier: 50843558

GI number: 50843558

Start: 2293195

End: 2294427

Strand: Direct

Name: kgtP [H]

Synonym: PPA2120

Alternate gene names: 50843558

Gene position: 2293195-2294427 (Clockwise)

Preceding gene: 50843557

Following gene: 50843559

Centisome position: 89.57

GC content: 58.23

Gene sequence:

>1233_bases
GTGTACACGTACACGACGTTCGCAGCCTATTTCGAGGACCAGTTCTTCTCGCCTGACGACCCGCACAAGAGCATCTACAT
CTACGCGATTTTCTGGGCGACCTTCATCATGAGGCCGTTCGGATCGTGGTTCTTCGGTCGCTGGGCCGACCGTCACGGAC
GCCGGTCCGCCCTCACCATGGCAGTGACAATCATGGCCGTCGGCTCCCTCGTCATCTCCGTGTTGCCCACCCGAGGCACC
ATCGGAGTGGGGGCCCCGATCCTGTTGCTCATCTGTCGTATCGTGCAAGGATTCGCCACAGGAGGTGAGTACGGCACCTC
GGCCACCTACATGTCAGAAGCCTCCACCAAGGGACACCGCGGTTTCTTCTCCTCGTTCCAGTACTTCACCCTCGTGGGTG
GTTTAGTGACAGCCCAGGCCGTCCTACTCCTGCTGTCCTCCCTCATGAGTGAGGCCACGATCCATGTCTGGGGATGGCGC
ATCCCATTCTTCATCGGTGGCGTCGCAGCGCTCATCGTTTTCTTCATGCAGAAGACGATGGATGAATCGCTGAGTACCGA
ACATCTTAAATCCGTGCGGTCGGGTGAGGACAGCTCGTCAGGAAGTCTGAAAGAGCTCTTTGAAAACCATCTCGGTAACT
TCCTCCTGGCTTTTACCATCGCCAGCGGCGGTACCCTGTGCTTCTATGTCTACACCGTCAATGCACCGAATATCGTCCAT
TCGACTTTCAGCAGCACTCCGATGGCGGCAACCGCGGTCAACCTCATCGCCCTATTCATCCTCATGCTCGGGCAACCGCT
GGGAGGCCTCATCGGTGACCGGATCGGACGCAAACCGGTGTTGCTCTTCTTTGCCGCCGGTGGAGTCGTTTATACCTGGG
TTCTCGTGACCTGTCTGTCGCATGCCCGCAAGCCCGTAACTGCATTCCTCATGCTGCTCATGGGCTATATCTTCCTTACC
GGATATACCTCGATCTCCGCAGTTGTGAAGTCCGACCTTTTCCCAACCGATGTCAGGGCGCTGGGTGTTGGGATGGCCCA
TGGTTTCGCGAATTCCCTGTTCGGCGGCACGGCTCCACTCATTTACGTGTGGGCGAAACAGGGTGACGCGATCCCCGCCT
TCATCGGGTATGTGACGGCGCTGTCCGCGGTCACCTTCCTTACGATCCTCTTCGGGCTGACCACACGGACTGCGTCACAC
CTGGATGCGCCGACGAAGGGTGGGCACGACTAA

Upstream 100 bases:

>100_bases
GAGCACCAGGTCAGCATCAGTACGCAGCGAATCGGACGTGTACCGCGCAGTATGGAACACCGTCAAGGGGTCGATGGGCA
ACCTCGTCGAGTAGTTCGAC

Downstream 100 bases:

>100_bases
CAAAGCGGCAGTTACTCGCCCCGGTACGCGGTGATTTCTCCAACGTTTCAGGCGGTCGAACACGATGCTACGAAGCCAAA
CTGCCGACGGCGAGGCCCGG

Product: dicarboxylate transport protein / putative alpha-ketoglutarate permease

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MYTYTTFAAYFEDQFFSPDDPHKSIYIYAIFWATFIMRPFGSWFFGRWADRHGRRSALTMAVTIMAVGSLVISVLPTRGT
IGVGAPILLLICRIVQGFATGGEYGTSATYMSEASTKGHRGFFSSFQYFTLVGGLVTAQAVLLLLSSLMSEATIHVWGWR
IPFFIGGVAALIVFFMQKTMDESLSTEHLKSVRSGEDSSSGSLKELFENHLGNFLLAFTIASGGTLCFYVYTVNAPNIVH
STFSSTPMAATAVNLIALFILMLGQPLGGLIGDRIGRKPVLLFFAAGGVVYTWVLVTCLSHARKPVTAFLMLLMGYIFLT
GYTSISAVVKSDLFPTDVRALGVGMAHGFANSLFGGTAPLIYVWAKQGDAIPAFIGYVTALSAVTFLTILFGLTTRTASH
LDAPTKGGHD

Sequences:

>Translated_410_residues
MYTYTTFAAYFEDQFFSPDDPHKSIYIYAIFWATFIMRPFGSWFFGRWADRHGRRSALTMAVTIMAVGSLVISVLPTRGT
IGVGAPILLLICRIVQGFATGGEYGTSATYMSEASTKGHRGFFSSFQYFTLVGGLVTAQAVLLLLSSLMSEATIHVWGWR
IPFFIGGVAALIVFFMQKTMDESLSTEHLKSVRSGEDSSSGSLKELFENHLGNFLLAFTIASGGTLCFYVYTVNAPNIVH
STFSSTPMAATAVNLIALFILMLGQPLGGLIGDRIGRKPVLLFFAAGGVVYTWVLVTCLSHARKPVTAFLMLLMGYIFLT
GYTSISAVVKSDLFPTDVRALGVGMAHGFANSLFGGTAPLIYVWAKQGDAIPAFIGYVTALSAVTFLTILFGLTTRTASH
LDAPTKGGHD
>Mature_410_residues
MYTYTTFAAYFEDQFFSPDDPHKSIYIYAIFWATFIMRPFGSWFFGRWADRHGRRSALTMAVTIMAVGSLVISVLPTRGT
IGVGAPILLLICRIVQGFATGGEYGTSATYMSEASTKGHRGFFSSFQYFTLVGGLVTAQAVLLLLSSLMSEATIHVWGWR
IPFFIGGVAALIVFFMQKTMDESLSTEHLKSVRSGEDSSSGSLKELFENHLGNFLLAFTIASGGTLCFYVYTVNAPNIVH
STFSSTPMAATAVNLIALFILMLGQPLGGLIGDRIGRKPVLLFFAAGGVVYTWVLVTCLSHARKPVTAFLMLLMGYIFLT
GYTSISAVVKSDLFPTDVRALGVGMAHGFANSLFGGTAPLIYVWAKQGDAIPAFIGYVTALSAVTFLTILFGLTTRTASH
LDAPTKGGHD

Specific function: Uptake of alpha-ketoglutarate across the boundary membrane with the concomitant import of a cation (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=390, Percent_Identity=38.7179487179487, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI1790550, Length=417, Percent_Identity=29.2565947242206, Blast_Score=168, Evalue=6e-43,
Organism=Escherichia coli, GI1788292, Length=357, Percent_Identity=26.6106442577031, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1789941, Length=414, Percent_Identity=28.743961352657, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI87082404, Length=375, Percent_Identity=25.6, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI87082231, Length=206, Percent_Identity=30.0970873786408, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 44365; Mature: 44365

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYTYTTFAAYFEDQFFSPDDPHKSIYIYAIFWATFIMRPFGSWFFGRWADRHGRRSALTM
CCEEHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
AVTIMAVGSLVISVLPTRGTIGVGAPILLLICRIVQGFATGGEYGTSATYMSEASTKGHR
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCH
GFFSSFQYFTLVGGLVTAQAVLLLLSSLMSEATIHVWGWRIPFFIGGVAALIVFFMQKTM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECHHHHHHHHHHHHHHHHHHHHH
DESLSTEHLKSVRSGEDSSSGSLKELFENHLGNFLLAFTIASGGTLCFYVYTVNAPNIVH
HHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCEEEEEEECCCCEEEEEEEEECCCHHHH
STFSSTPMAATAVNLIALFILMLGQPLGGLIGDRIGRKPVLLFFAAGGVVYTWVLVTCLS
HHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHH
HARKPVTAFLMLLMGYIFLTGYTSISAVVKSDLFPTDVRALGVGMAHGFANSLFGGTAPL
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCE
IYVWAKQGDAIPAFIGYVTALSAVTFLTILFGLTTRTASHLDAPTKGGHD
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MYTYTTFAAYFEDQFFSPDDPHKSIYIYAIFWATFIMRPFGSWFFGRWADRHGRRSALTM
CCEEHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
AVTIMAVGSLVISVLPTRGTIGVGAPILLLICRIVQGFATGGEYGTSATYMSEASTKGHR
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCH
GFFSSFQYFTLVGGLVTAQAVLLLLSSLMSEATIHVWGWRIPFFIGGVAALIVFFMQKTM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECHHHHHHHHHHHHHHHHHHHHH
DESLSTEHLKSVRSGEDSSSGSLKELFENHLGNFLLAFTIASGGTLCFYVYTVNAPNIVH
HHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCEEEEEEECCCCEEEEEEEEECCCHHHH
STFSSTPMAATAVNLIALFILMLGQPLGGLIGDRIGRKPVLLFFAAGGVVYTWVLVTCLS
HHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHH
HARKPVTAFLMLLMGYIFLTGYTSISAVVKSDLFPTDVRALGVGMAHGFANSLFGGTAPL
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCE
IYVWAKQGDAIPAFIGYVTALSAVTFLTILFGLTTRTASHLDAPTKGGHD
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2118499; 9205837; 9278503; 1709493; 2190187; 2053984; 8419306 [H]