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The map label for this gene is iaaA [H]

Identifier: 50843552

GI number: 50843552

Start: 2288345

End: 2289310

Strand: Direct

Name: iaaA [H]

Synonym: PPA2114

Alternate gene names: 50843552

Gene position: 2288345-2289310 (Clockwise)

Preceding gene: 50843551

Following gene: 50843554

Centisome position: 89.38

GC content: 63.04

Gene sequence:

>966_bases
GTGGATAAACCCTTAGTGACATCACCCCGTGACCAGCCTTACGATCCTGTTATGACCAGTCACGTCGTCGATCAGCCCGG
CCCTACCCTCGTCATTCATGGTGGGGCCGGAAACGTCCCGTCACGTACTGGAGAGGAAGCCGAGAAGTTTCGTCGCTCCT
TGCGTCAGGCCTTTGCAGCCGGGCAGCGAGCCCTCGACGTAAGTGCTTGTGACGCCGTCATCGCAGCCGTTACGGCGATG
GAGGACGATCCACTGTTCAACGCCGGGCGAGGTGGGGTTCTCACCGCAAACGGAACCGTCGAAACCGACGCCGCCGTCAT
GACTGGAGACGGACACGCCGGGGCGGTGTGCTGCTCACGTCACGCCCGCAACCCGATTCAACTGGCGCATAAAGTTCTGA
CGGATTCACCCCATGTGCTGCTGTGTGACCCGCCCGAACGGGTCTGTCAAGACTGGGGTCTTGAGATCGCGCCGCCGGAG
TACTTCGTCACCCAAGCGCGCCGCGAACAGTTAGAGCAGGTGCTAGCCAGACGACAGCACGCCCCGAAACACGGCACCGT
CGGGGCGGTTGCCCGAGATCGTTATGGACACCTAGCTGCGGCCACCTCAACCGGTGGGATCGTCGCTTCGTCGCAGGGAC
GCATCGGGGACTCTCCGGTCATCGGGGCCGGCACTTTCGCGCGCGACGGAGTCGTGGCAGTCTCCTGCACCGGCGATGGT
GAGGCCTTCCTCCAAGGGGTAGTAGCTCATGAGGTTGACGCGCAGATGAGACTGGCAGGACAACCCGTCGACCGGGCAGC
TCTGGGAGCCCTCACCGACGAGGTCTCATCGCGCCTCACTGGCCACGCCCCAGCAACCGATGGCCTCATCGCCGTCGACT
CTTGTGAACAATTGGTAGTCTGTCACGTGTCAGCGTCGATGCTGGCTGCATGGCCAGACCACGGCGAGGTTCTCACCTCG
GTGTAG

Upstream 100 bases:

>100_bases
TGCGACCGCGGCTGGTGAGTTATCCACAGGAGACCGGTGGAAAACCGGCCCGGCTGGGGAAAACACATCAATGTCGTTCC
CACAGGCGGGGTGAGAACCA

Downstream 100 bases:

>100_bases
TGAAGGTCAGCGCACCACGGGAAGATCGGGCCCAAACTCCAGCATCTGTTCGACGGCCCAGGGACTCAGCAGGGCCGGCA
TTGACCGGGCCAAGTCATAC

Product: putative asparaginase

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MDKPLVTSPRDQPYDPVMTSHVVDQPGPTLVIHGGAGNVPSRTGEEAEKFRRSLRQAFAAGQRALDVSACDAVIAAVTAM
EDDPLFNAGRGGVLTANGTVETDAAVMTGDGHAGAVCCSRHARNPIQLAHKVLTDSPHVLLCDPPERVCQDWGLEIAPPE
YFVTQARREQLEQVLARRQHAPKHGTVGAVARDRYGHLAAATSTGGIVASSQGRIGDSPVIGAGTFARDGVVAVSCTGDG
EAFLQGVVAHEVDAQMRLAGQPVDRAALGALTDEVSSRLTGHAPATDGLIAVDSCEQLVVCHVSASMLAAWPDHGEVLTS
V

Sequences:

>Translated_321_residues
MDKPLVTSPRDQPYDPVMTSHVVDQPGPTLVIHGGAGNVPSRTGEEAEKFRRSLRQAFAAGQRALDVSACDAVIAAVTAM
EDDPLFNAGRGGVLTANGTVETDAAVMTGDGHAGAVCCSRHARNPIQLAHKVLTDSPHVLLCDPPERVCQDWGLEIAPPE
YFVTQARREQLEQVLARRQHAPKHGTVGAVARDRYGHLAAATSTGGIVASSQGRIGDSPVIGAGTFARDGVVAVSCTGDG
EAFLQGVVAHEVDAQMRLAGQPVDRAALGALTDEVSSRLTGHAPATDGLIAVDSCEQLVVCHVSASMLAAWPDHGEVLTS
V
>Mature_321_residues
MDKPLVTSPRDQPYDPVMTSHVVDQPGPTLVIHGGAGNVPSRTGEEAEKFRRSLRQAFAAGQRALDVSACDAVIAAVTAM
EDDPLFNAGRGGVLTANGTVETDAAVMTGDGHAGAVCCSRHARNPIQLAHKVLTDSPHVLLCDPPERVCQDWGLEIAPPE
YFVTQARREQLEQVLARRQHAPKHGTVGAVARDRYGHLAAATSTGGIVASSQGRIGDSPVIGAGTFARDGVVAVSCTGDG
EAFLQGVVAHEVDAQMRLAGQPVDRAALGALTDEVSSRLTGHAPATDGLIAVDSCEQLVVCHVSASMLAAWPDHGEVLTS
V

Specific function: Degrades proteins damaged by L-isoaspartyl residue formation (also known as beta-Asp residues). Degrades L- isoaspartyl-containing di- and tripeptides. Acts best on iso-Asp- Leu, followed by iso-Asp-Ala, -His and to a lesser extent iso-Asp- Lys, -Phe and

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=289, Percent_Identity=39.4463667820069, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI145275200, Length=289, Percent_Identity=39.4463667820069, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI98991769, Length=270, Percent_Identity=29.2592592592593, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1787050, Length=310, Percent_Identity=37.741935483871, Blast_Score=163, Evalue=1e-41,
Organism=Drosophila melanogaster, GI18921183, Length=322, Percent_Identity=31.3664596273292, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI21357893, Length=268, Percent_Identity=28.7313432835821, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 33449; Mature: 33449

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKPLVTSPRDQPYDPVMTSHVVDQPGPTLVIHGGAGNVPSRTGEEAEKFRRSLRQAFAA
CCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHC
GQRALDVSACDAVIAAVTAMEDDPLFNAGRGGVLTANGTVETDAAVMTGDGHAGAVCCSR
CCHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCEECCCEEEECCCCCCHHHHHH
HARNPIQLAHKVLTDSPHVLLCDPPERVCQDWGLEIAPPEYFVTQARREQLEQVLARRQH
HCCCHHHHHHHHHCCCCCEEEECCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
APKHGTVGAVARDRYGHLAAATSTGGIVASSQGRIGDSPVIGAGTFARDGVVAVSCTGDG
CCCCCCCCHHHHHCCCCEEEECCCCCEEECCCCCCCCCCEECCCCCCCCCEEEEEECCCH
EAFLQGVVAHEVDAQMRLAGQPVDRAALGALTDEVSSRLTGHAPATDGLIAVDSCEQLVV
HHHHHHHHHHHHCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCEEEE
CHVSASMLAAWPDHGEVLTSV
EEECHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MDKPLVTSPRDQPYDPVMTSHVVDQPGPTLVIHGGAGNVPSRTGEEAEKFRRSLRQAFAA
CCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHC
GQRALDVSACDAVIAAVTAMEDDPLFNAGRGGVLTANGTVETDAAVMTGDGHAGAVCCSR
CCHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCEECCCEEEECCCCCCHHHHHH
HARNPIQLAHKVLTDSPHVLLCDPPERVCQDWGLEIAPPEYFVTQARREQLEQVLARRQH
HCCCHHHHHHHHHCCCCCEEEECCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
APKHGTVGAVARDRYGHLAAATSTGGIVASSQGRIGDSPVIGAGTFARDGVVAVSCTGDG
CCCCCCCCHHHHHCCCCEEEECCCCCEEECCCCCCCCCCEECCCCCCCCCEEEEEECCCH
EAFLQGVVAHEVDAQMRLAGQPVDRAALGALTDEVSSRLTGHAPATDGLIAVDSCEQLVV
HHHHHHHHHHHHCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCEEEE
CHVSASMLAAWPDHGEVLTSV
EEECHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]