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The map label for this gene is gutB [H]

Identifier: 50843479

GI number: 50843479

Start: 2209096

End: 2210139

Strand: Reverse

Name: gutB [H]

Synonym: PPA2035

Alternate gene names: 50843479

Gene position: 2210139-2209096 (Counterclockwise)

Preceding gene: 50843480

Following gene: 50843478

Centisome position: 86.32

GC content: 61.11

Gene sequence:

>1044_bases
ATGACAGAAACCATGCTTGCTGAACGTTTCCACGCCGCAGATCGGTCTATCACCCTCGAAGATATCCCGATCCCGCACCC
GGGACCCGGTGAGGTGCTTGTCAAGGTCGCTTACTGCGGTATTTGCCATTCTGACCTGAGTCTCATTAGCGGTGCGTTCC
CTGCAGCTCTGCCAGTAGTGACTCAGGGCCATGAGGCATCCGGAACCATCGTCGAGCTCGGTGCGGGCGTCATCGGTTGG
AAGGAGGGTGACCGCGTCATCCCATCCGCCGGTCGACCCTGTCTCAAGTGTCGCAAGTGCCGCCGCGGTGCCTTCACGGA
CTGTCTGGATCTGAACCTCATGGCTTTTGCCTACGACGGCGCATGGGCTGAGTACCACATTGCGCAGGCCTCCGGTATGA
CGAAGATCCCTGACAACGTCCCACTGGACCAGGCCGCTCTGCTCGCAGACGCCGTATCTACCCCGTATGCTGCCGTCGTG
CGTACCGGCAAGGTCCACATGGGCAATGCCGTGGCCGTGTGGGGCGTCGGTGGTGTCGGAACCCACCTCGTCCAACTAGC
CAAGCTTGCCGGCGGCGTCCCCGTCATTGCTATTGATCTCAATGACGCTGTCCTCGAGCGCGCTAAGCGAGTCGGCGCCG
ACCACACTCTGCGTAGCGACGACCCCGAGCTCATGCAGAAAATCGAGGACATCACTCATGGCCGCATGATCGACGTTGCC
TTCGACGCCGTCGGCATCAAGCCGACGCTGCGTGCCTGCGTCGACTCCCTCGACGTCGATGGCAAGGCGGTTTCGGTTGG
GCTGTCCGCCCAGGACATCGACGCTGGTCCATTCCTCAACTTCAACCTGCAACGCAAGCAGGTCCTTGGCCACCTTGGTT
ACAAGTCTCAGGACATCGCCCTGCTCGCCGAGATGTTGTCCTATCACCGCCTGGACCTCACGGAGTCCATCTCAAAGGTC
ATCCCGTTGAAGGATGTCGAGAGCGGCATCGCGGAGCTCGAGTCCCACCAGGGCAACCCGATCCGTATCCTGGTCAAGCC
CTGA

Upstream 100 bases:

>100_bases
GCTGAAGTAGCGAGCAAACCGCAGGATGACGTATTAACCTCGGCATCAATAGCGTTGGCAGTTGGCAATGGGGTCGGCGC
GCTCACAGAGGGGATCAACG

Downstream 100 bases:

>100_bases
TGGAGCGCATCACGGGTGCCGTCGCGATTCGTTGTGGCGGCACCTTCGCGTTGGGCGGCATCAATCCATGCAGCTGTGTG
CAACCTCTGTGTCTGAGCGA

Product: putative zinc-binding dehydrogenase

Products: NA

Alternate protein names: Glucitol dehydrogenase; L-iditol 2-dehydrogenase [H]

Number of amino acids: Translated: 347; Mature: 346

Protein sequence:

>347_residues
MTETMLAERFHAADRSITLEDIPIPHPGPGEVLVKVAYCGICHSDLSLISGAFPAALPVVTQGHEASGTIVELGAGVIGW
KEGDRVIPSAGRPCLKCRKCRRGAFTDCLDLNLMAFAYDGAWAEYHIAQASGMTKIPDNVPLDQAALLADAVSTPYAAVV
RTGKVHMGNAVAVWGVGGVGTHLVQLAKLAGGVPVIAIDLNDAVLERAKRVGADHTLRSDDPELMQKIEDITHGRMIDVA
FDAVGIKPTLRACVDSLDVDGKAVSVGLSAQDIDAGPFLNFNLQRKQVLGHLGYKSQDIALLAEMLSYHRLDLTESISKV
IPLKDVESGIAELESHQGNPIRILVKP

Sequences:

>Translated_347_residues
MTETMLAERFHAADRSITLEDIPIPHPGPGEVLVKVAYCGICHSDLSLISGAFPAALPVVTQGHEASGTIVELGAGVIGW
KEGDRVIPSAGRPCLKCRKCRRGAFTDCLDLNLMAFAYDGAWAEYHIAQASGMTKIPDNVPLDQAALLADAVSTPYAAVV
RTGKVHMGNAVAVWGVGGVGTHLVQLAKLAGGVPVIAIDLNDAVLERAKRVGADHTLRSDDPELMQKIEDITHGRMIDVA
FDAVGIKPTLRACVDSLDVDGKAVSVGLSAQDIDAGPFLNFNLQRKQVLGHLGYKSQDIALLAEMLSYHRLDLTESISKV
IPLKDVESGIAELESHQGNPIRILVKP
>Mature_346_residues
TETMLAERFHAADRSITLEDIPIPHPGPGEVLVKVAYCGICHSDLSLISGAFPAALPVVTQGHEASGTIVELGAGVIGWK
EGDRVIPSAGRPCLKCRKCRRGAFTDCLDLNLMAFAYDGAWAEYHIAQASGMTKIPDNVPLDQAALLADAVSTPYAAVVR
TGKVHMGNAVAVWGVGGVGTHLVQLAKLAGGVPVIAIDLNDAVLERAKRVGADHTLRSDDPELMQKIEDITHGRMIDVAF
DAVGIKPTLRACVDSLDVDGKAVSVGLSAQDIDAGPFLNFNLQRKQVLGHLGYKSQDIALLAEMLSYHRLDLTESISKVI
PLKDVESGIAELESHQGNPIRILVKP

Specific function: Reduces glucitol to fructose [H]

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI71743840, Length=350, Percent_Identity=30.5714285714286, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI262073058, Length=348, Percent_Identity=30.7471264367816, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI34577061, Length=358, Percent_Identity=28.4916201117318, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI4501933, Length=359, Percent_Identity=27.8551532033426, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI4501929, Length=361, Percent_Identity=28.2548476454294, Blast_Score=131, Evalue=9e-31,
Organism=Homo sapiens, GI71565154, Length=362, Percent_Identity=26.5193370165746, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI71565152, Length=366, Percent_Identity=26.2295081967213, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI156627571, Length=340, Percent_Identity=29.1176470588235, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI156523966, Length=358, Percent_Identity=25.9776536312849, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI4501939, Length=350, Percent_Identity=25.7142857142857, Blast_Score=105, Evalue=9e-23,
Organism=Escherichia coli, GI1788073, Length=329, Percent_Identity=28.5714285714286, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1786552, Length=364, Percent_Identity=28.2967032967033, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI87082125, Length=280, Percent_Identity=31.4285714285714, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1790045, Length=337, Percent_Identity=26.7062314540059, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1788075, Length=336, Percent_Identity=24.4047619047619, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI87081918, Length=306, Percent_Identity=30.3921568627451, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1787863, Length=294, Percent_Identity=30.2721088435374, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788407, Length=327, Percent_Identity=25.0764525993884, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1786825, Length=289, Percent_Identity=26.643598615917, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790718, Length=250, Percent_Identity=24.4, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI87082401, Length=245, Percent_Identity=28.1632653061224, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786518, Length=299, Percent_Identity=24.4147157190635, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI226510992, Length=307, Percent_Identity=24.1042345276873, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17562878, Length=248, Percent_Identity=34.2741935483871, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI25146526, Length=364, Percent_Identity=27.7472527472527, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17562876, Length=282, Percent_Identity=32.6241134751773, Blast_Score=127, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI71997431, Length=366, Percent_Identity=27.5956284153005, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17565904, Length=347, Percent_Identity=26.8011527377522, Blast_Score=117, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17562582, Length=289, Percent_Identity=30.1038062283737, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71988145, Length=202, Percent_Identity=32.1782178217822, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17562584, Length=268, Percent_Identity=29.1044776119403, Blast_Score=85, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6320033, Length=370, Percent_Identity=26.2162162162162, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6319621, Length=339, Percent_Identity=30.3834808259587, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6323961, Length=344, Percent_Identity=28.7790697674419, Blast_Score=118, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6324486, Length=344, Percent_Identity=28.7790697674419, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6323729, Length=230, Percent_Identity=32.1739130434783, Blast_Score=112, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6323099, Length=342, Percent_Identity=26.6081871345029, Blast_Score=109, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6319257, Length=358, Percent_Identity=25.6983240223464, Blast_Score=102, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6319955, Length=347, Percent_Identity=25.9365994236311, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6322619, Length=347, Percent_Identity=25.9365994236311, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6319258, Length=379, Percent_Identity=25.5936675461741, Blast_Score=90, Evalue=6e-19,
Organism=Drosophila melanogaster, GI17737895, Length=365, Percent_Identity=28.4931506849315, Blast_Score=138, Evalue=6e-33,
Organism=Drosophila melanogaster, GI17737897, Length=317, Percent_Identity=30.9148264984227, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI17137530, Length=337, Percent_Identity=27.893175074184, Blast_Score=106, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.14 [H]

Molecular weight: Translated: 36940; Mature: 36809

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00059 ADH_ZINC ; PS00683 RHODANESE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETMLAERFHAADRSITLEDIPIPHPGPGEVLVKVAYCGICHSDLSLISGAFPAALPVV
CCHHHHHHHHHHCCCCEEEECCCCCCCCCHHHEEEHHHHHCCHHHHHHHHCCCHHHCCEE
TQGHEASGTIVELGAGVIGWKEGDRVIPSAGRPCLKCRKCRRGAFTDCLDLNLMAFAYDG
ECCCCCCCCEEEECCCEEEECCCCEECCCCCCHHHHHHHHCCCCCCHHHCCEEEEEEECC
AWAEYHIAQASGMTKIPDNVPLDQAALLADAVSTPYAAVVRTGKVHMGNAVAVWGVGGVG
CCCEEEEHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHHEECCCEEECCEEEEECCCCHH
THLVQLAKLAGGVPVIAIDLNDAVLERAKRVGADHTLRSDDPELMQKIEDITHGRMIDVA
HHHHHHHHHHCCCEEEEEECCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCEEEEE
FDAVGIKPTLRACVDSLDVDGKAVSVGLSAQDIDAGPFLNFNLQRKQVLGHLGYKSQDIA
ECCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEECCCHHHHHHHHCCCCCHHHH
LLAEMLSYHRLDLTESISKVIPLKDVESGIAELESHQGNPIRILVKP
HHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEECC
>Mature Secondary Structure 
TETMLAERFHAADRSITLEDIPIPHPGPGEVLVKVAYCGICHSDLSLISGAFPAALPVV
CHHHHHHHHHHCCCCEEEECCCCCCCCCHHHEEEHHHHHCCHHHHHHHHCCCHHHCCEE
TQGHEASGTIVELGAGVIGWKEGDRVIPSAGRPCLKCRKCRRGAFTDCLDLNLMAFAYDG
ECCCCCCCCEEEECCCEEEECCCCEECCCCCCHHHHHHHHCCCCCCHHHCCEEEEEEECC
AWAEYHIAQASGMTKIPDNVPLDQAALLADAVSTPYAAVVRTGKVHMGNAVAVWGVGGVG
CCCEEEEHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHHEECCCEEECCEEEEECCCCHH
THLVQLAKLAGGVPVIAIDLNDAVLERAKRVGADHTLRSDDPELMQKIEDITHGRMIDVA
HHHHHHHHHHCCCEEEEEECCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCEEEEE
FDAVGIKPTLRACVDSLDVDGKAVSVGLSAQDIDAGPFLNFNLQRKQVLGHLGYKSQDIA
ECCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEECCCHHHHHHHHCCCCCHHHH
LLAEMLSYHRLDLTESISKVIPLKDVESGIAELESHQGNPIRILVKP
HHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10086842; 11058132 [H]