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The map label for this gene is brnQ [H]

Identifier: 50843467

GI number: 50843467

Start: 2194985

End: 2196343

Strand: Direct

Name: brnQ [H]

Synonym: PPA2022

Alternate gene names: 50843467

Gene position: 2194985-2196343 (Clockwise)

Preceding gene: 50843464

Following gene: 50843472

Centisome position: 85.73

GC content: 63.87

Gene sequence:

>1359_bases
ATGTCCGATCTGCGAACCGTCCCCAAGCACACCGCCCTCCTCATCGGGCTCATGATATTTGGGCTATTTTTCGGCGCCGG
AAACCTCATCTTCCCGGTCGAGCTAGGGCGCCTCGCCGGCCACAGCGCCCCCGTAACCGCCGCAGGATTTCTCGTCACCG
CAGTCGGGATCCCCATCGTGGCCATCATCTCTTCCGCCGTGTCGGGATGCTCCAGCGTCCACGGCATGACCTCGCGGGTG
TCCTCTCAATACGCCACGATCTTCACCTGCCTGCTGTACCTGACCATCGGCCCAGCCTTCGCCATCCCGAGAACGGCGAC
CGTCTCCTACGAAGTCGGCGTGGCGCCCTTTATCCAAGGCCACGGCCCGGCACTGCCGATCTTCACCATCGTGTTCTTCG
CGATGACTCTGGTCGCAGCCCTCAAACCCGGAAAGCTCATGGACTGGGTAGGGCGATATCTGACCCCACTGTTCCTTGCC
CTGCTAGCCATCCTCATCGTGGCAACCCTCATCCATCCCATGCCCACCGTCGCGAATCGTCCCCCGCAGCCCCCGTACGA
TCACCAGCCCTTCATGCACGGCTTCCTCGACGGCTACAACACCATGGACGCGCTGGCGTCCCTCGCCTTCGCCATCGTCA
TCATTGAAGCAATCAATCAACGCGGCTCGACGGGCCGTCGCGATGTCACCCATATCACAATCCGCGCCGGGCTGGTTGCC
ACCATCCCGCTGACCCTCGTCTACGTATCCCTGGCATGGCTCGGGCGCACGTCCACCCTCGTCGTCCCTAACGCTGCCAA
TGGCGGCGTCGTCCTGGCTGGGGTTTCACGCCACTACTACGGCACGGCCGGTCAGGTGTTGATAGCCGCCATCGTGCTGG
TAGCCTGCCTCAAAACAGCGATCGGTTTGGTCGTGGCCTGTGCCGAGATCTTTCGTCGCATGTTCCCGGCGGGGCCGTCA
GTGCGAAGCTGGGTCGTCATCTTCAGCGTGGCGTCAGCCATCATCGCCAACGCCGGTCTGACGACGATCATCGCCTGGTC
GACCCCCGTCCTCATGTTCCTCTACCCGTTGGCAATCACGGCGATCATTTTGGGGCTGCTTGACCCGTGGCTGGGTGCCA
GCCGCCTCCCCCACCGCATCATGACCGGATGCGTCGTCCTGGCTGCCCTGCCAGACCTGGTGCGCGCCCTGCCGATCCAA
GGGTCAGGAACCCGCGCTATCACCCACGTGGCCGACATGGTCCTACCCTGGGCGTCCGACGGTCTCGGTTGGGGGCTTCC
CGCCATTATCGGTCTGGCCGCCGGTTGGGGAGCCAGTCTCATCTTGGGTCGCCCCCGTCGTTGGGGCACGAGTAGCTGA

Upstream 100 bases:

>100_bases
TCCCAATTGTCAGATGACACGAACGACCCTCAGATGAACGCTCGTTCTCACCTATAGGAAAAGCGGAAGCTACCGCCACC
ACCCTGGCAAGATATTCCCC

Downstream 100 bases:

>100_bases
GTGGCTTTACGCTTGCGAGAGCAAGTCTTCGAGCTTGACCAGGGTGTCGTGCCAGTCGTCGACCGCGACGCACGGAATAC
CCATCGCCTTGACAGGATAG

Product: branched-chain amino acid transport permease

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier [H]

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MSDLRTVPKHTALLIGLMIFGLFFGAGNLIFPVELGRLAGHSAPVTAAGFLVTAVGIPIVAIISSAVSGCSSVHGMTSRV
SSQYATIFTCLLYLTIGPAFAIPRTATVSYEVGVAPFIQGHGPALPIFTIVFFAMTLVAALKPGKLMDWVGRYLTPLFLA
LLAILIVATLIHPMPTVANRPPQPPYDHQPFMHGFLDGYNTMDALASLAFAIVIIEAINQRGSTGRRDVTHITIRAGLVA
TIPLTLVYVSLAWLGRTSTLVVPNAANGGVVLAGVSRHYYGTAGQVLIAAIVLVACLKTAIGLVVACAEIFRRMFPAGPS
VRSWVVIFSVASAIIANAGLTTIIAWSTPVLMFLYPLAITAIILGLLDPWLGASRLPHRIMTGCVVLAALPDLVRALPIQ
GSGTRAITHVADMVLPWASDGLGWGLPAIIGLAAGWGASLILGRPRRWGTSS

Sequences:

>Translated_452_residues
MSDLRTVPKHTALLIGLMIFGLFFGAGNLIFPVELGRLAGHSAPVTAAGFLVTAVGIPIVAIISSAVSGCSSVHGMTSRV
SSQYATIFTCLLYLTIGPAFAIPRTATVSYEVGVAPFIQGHGPALPIFTIVFFAMTLVAALKPGKLMDWVGRYLTPLFLA
LLAILIVATLIHPMPTVANRPPQPPYDHQPFMHGFLDGYNTMDALASLAFAIVIIEAINQRGSTGRRDVTHITIRAGLVA
TIPLTLVYVSLAWLGRTSTLVVPNAANGGVVLAGVSRHYYGTAGQVLIAAIVLVACLKTAIGLVVACAEIFRRMFPAGPS
VRSWVVIFSVASAIIANAGLTTIIAWSTPVLMFLYPLAITAIILGLLDPWLGASRLPHRIMTGCVVLAALPDLVRALPIQ
GSGTRAITHVADMVLPWASDGLGWGLPAIIGLAAGWGASLILGRPRRWGTSS
>Mature_451_residues
SDLRTVPKHTALLIGLMIFGLFFGAGNLIFPVELGRLAGHSAPVTAAGFLVTAVGIPIVAIISSAVSGCSSVHGMTSRVS
SQYATIFTCLLYLTIGPAFAIPRTATVSYEVGVAPFIQGHGPALPIFTIVFFAMTLVAALKPGKLMDWVGRYLTPLFLAL
LAILIVATLIHPMPTVANRPPQPPYDHQPFMHGFLDGYNTMDALASLAFAIVIIEAINQRGSTGRRDVTHITIRAGLVAT
IPLTLVYVSLAWLGRTSTLVVPNAANGGVVLAGVSRHYYGTAGQVLIAAIVLVACLKTAIGLVVACAEIFRRMFPAGPSV
RSWVVIFSVASAIIANAGLTTIIAWSTPVLMFLYPLAITAIILGLLDPWLGASRLPHRIMTGCVVLAALPDLVRALPIQG
SGTRAITHVADMVLPWASDGLGWGLPAIIGLAAGWGASLILGRPRRWGTSS

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=416, Percent_Identity=37.2596153846154, Blast_Score=250, Evalue=1e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 47518; Mature: 47387

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLRTVPKHTALLIGLMIFGLFFGAGNLIFPVELGRLAGHSAPVTAAGFLVTAVGIPIV
CCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHCCCCCCCHHHHHHHHHHHHHHH
AIISSAVSGCSSVHGMTSRVSSQYATIFTCLLYLTIGPAFAIPRTATVSYEVGVAPFIQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCEEEEECCCCHHCCC
HGPALPIFTIVFFAMTLVAALKPGKLMDWVGRYLTPLFLALLAILIVATLIHPMPTVANR
CCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCC
PPQPPYDHQPFMHGFLDGYNTMDALASLAFAIVIIEAINQRGSTGRRDVTHITIRAGLVA
CCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHH
TIPLTLVYVSLAWLGRTSTLVVPNAANGGVVLAGVSRHYYGTAGQVLIAAIVLVACLKTA
HHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEECCHHHCCCHHHHHHHHHHHHHHHHHH
IGLVVACAEIFRRMFPAGPSVRSWVVIFSVASAIIANAGLTTIIAWSTPVLMFLYPLAIT
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHEEEEHHHHHHHHHHHHHHH
AIILGLLDPWLGASRLPHRIMTGCVVLAALPDLVRALPIQGSGTRAITHVADMVLPWASD
HHHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCC
GLGWGLPAIIGLAAGWGASLILGRPRRWGTSS
CCCCCHHHHHHHHHCCCCHHHCCCCCCCCCCC
>Mature Secondary Structure 
SDLRTVPKHTALLIGLMIFGLFFGAGNLIFPVELGRLAGHSAPVTAAGFLVTAVGIPIV
CCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHCCCCCCCHHHHHHHHHHHHHHH
AIISSAVSGCSSVHGMTSRVSSQYATIFTCLLYLTIGPAFAIPRTATVSYEVGVAPFIQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCEEEEECCCCHHCCC
HGPALPIFTIVFFAMTLVAALKPGKLMDWVGRYLTPLFLALLAILIVATLIHPMPTVANR
CCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCC
PPQPPYDHQPFMHGFLDGYNTMDALASLAFAIVIIEAINQRGSTGRRDVTHITIRAGLVA
CCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHH
TIPLTLVYVSLAWLGRTSTLVVPNAANGGVVLAGVSRHYYGTAGQVLIAAIVLVACLKTA
HHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEECCHHHCCCHHHHHHHHHHHHHHHHHH
IGLVVACAEIFRRMFPAGPSVRSWVVIFSVASAIIANAGLTTIIAWSTPVLMFLYPLAIT
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHEEEEHHHHHHHHHHHHHHH
AIILGLLDPWLGASRLPHRIMTGCVVLAALPDLVRALPIQGSGTRAITHVADMVLPWASD
HHHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCC
GLGWGLPAIIGLAAGWGASLILGRPRRWGTSS
CCCCCHHHHHHHHHCCCCHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8544834 [H]