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The map label for this gene is menF [H]

Identifier: 50843389

GI number: 50843389

Start: 2099944

End: 2101230

Strand: Direct

Name: menF [H]

Synonym: PPA1940

Alternate gene names: 50843389

Gene position: 2099944-2101230 (Clockwise)

Preceding gene: 50843388

Following gene: 161621790

Centisome position: 82.02

GC content: 60.53

Gene sequence:

>1287_bases
ATGAGACGGTGCTGCCCATGTGGACATCTCGGTTATCCTGCCACCGTGATCGTTGACCCAGGTTCAGCCCGCCTATGTGC
CCGCACCGTCTCGATCTCGGACCCAGGTCCGCTCGAAAATTACATGACGCCGAACGACTCGGTGTGCTTTTTGCACCGCA
ACTACGGAATGGTTGGGCTAGGTGAGGTCACGCGATTCGAGACTGATTCCCCGGCCGCCGCCGATGTCTGGTGGGAGGCA
TTGTGCGATGATCTCGAGCACGAAACTGAGATGCCTGATGCTGAGGCCACTGGACCAGTCGCCTTCGGATCCTTCACGTT
TGACCCAGACCGGTCATCGTCACGGTCTGTCATGATCCTTCCCAAAACGATCATTGGACGCTGGCCCGGTTATTCGTGGC
TCACCCAACTCTCCTGGGACAGCATCACCGACGATTCTCCCCATCAGCAAAGCGTGCCCCGGCCCCCCGGGGCCGTGACG
GTACGTGACGGTGTCGTCAGTGAGCGAAGTTGGCGCCAAGTGGCGCGACTTGTCCAAGACATCATGAGCCCCGGAGAACT
CGACCAGATTGTCCTCATGCGTGACGTCGAAGCCAAAGCTTCCTCCTCTATCGACCCGCGATGGATCGTCGAGACTCTGC
GTCGCCAATTCCCCAACGCATATACCTATCTCGTCGAGGGATCCGTCGGCACCACGTCGAAACTCACCGTCGGGGTGAGC
AAGTCCTTAATCACATCCCGGGTACTGACGCACTCCCCCGCCACCGACGCCAATCACCAGACCCTGCTCACTGCGCTTCA
AGGCAAAGGGCCTCTGGCAGCCTATCATGACGAGATCGTTGGGCGAACCTGCGCACGTCTCGAACAATTCTGCGACACGG
TCCACGTTCCCGACTGCCCGGGATGTGTCTACAACGATGCCTCAACCTACCTCGTTACCGACGTCACTGGTATCCACTCC
CCCGATCAAGGATCCTGTCTGGCCCTCGTGCAGGCGCTGTGCCCGCCAGCTTTTGTCACCGGACTGCCACCACATTGCGC
CCTGTCCGTGCTGGCTGAGATTGAGCACGTCGACCGTGGCCGCGTCTCCGGCCCCACTGGCTGGGTCGATTCCTTGGGCA
ATGGCCAGTGGGTCACCGACTGCCGTGGCGGTCAGATCGACGCAACCGACCCTTCACTCGTCCATATCTTCTCTGGCCAC
CCCGTCGGCCATGACGACGACGCGGACGTCTTGTCGGCCGAGGCAGAGCATCGCATCGATGTGCTGCGGGACCTCTTCTT
GACGTAA

Upstream 100 bases:

>100_bases
ATGGTGGATCCCGCCCTCAACTGAGTCAACGCGCGTGGGCAGCGCCAACGGGGACGACCTCGTTTGGCATGGTTTGTCGT
CCCCGTTGGCGCTCATAGCG

Downstream 100 bases:

>100_bases
GTTGAGGCCTGACCATATGAAAGCGGTGGGACGCGCCGATAGCTGACGTCGATCCTGCCGGACGATTACCGCCGCTTACG
CTCGATGACCTTCTTCTCAG

Product: menaquinone-specific isochorismate synthase

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MRRCCPCGHLGYPATVIVDPGSARLCARTVSISDPGPLENYMTPNDSVCFLHRNYGMVGLGEVTRFETDSPAAADVWWEA
LCDDLEHETEMPDAEATGPVAFGSFTFDPDRSSSRSVMILPKTIIGRWPGYSWLTQLSWDSITDDSPHQQSVPRPPGAVT
VRDGVVSERSWRQVARLVQDIMSPGELDQIVLMRDVEAKASSSIDPRWIVETLRRQFPNAYTYLVEGSVGTTSKLTVGVS
KSLITSRVLTHSPATDANHQTLLTALQGKGPLAAYHDEIVGRTCARLEQFCDTVHVPDCPGCVYNDASTYLVTDVTGIHS
PDQGSCLALVQALCPPAFVTGLPPHCALSVLAEIEHVDRGRVSGPTGWVDSLGNGQWVTDCRGGQIDATDPSLVHIFSGH
PVGHDDDADVLSAEAEHRIDVLRDLFLT

Sequences:

>Translated_428_residues
MRRCCPCGHLGYPATVIVDPGSARLCARTVSISDPGPLENYMTPNDSVCFLHRNYGMVGLGEVTRFETDSPAAADVWWEA
LCDDLEHETEMPDAEATGPVAFGSFTFDPDRSSSRSVMILPKTIIGRWPGYSWLTQLSWDSITDDSPHQQSVPRPPGAVT
VRDGVVSERSWRQVARLVQDIMSPGELDQIVLMRDVEAKASSSIDPRWIVETLRRQFPNAYTYLVEGSVGTTSKLTVGVS
KSLITSRVLTHSPATDANHQTLLTALQGKGPLAAYHDEIVGRTCARLEQFCDTVHVPDCPGCVYNDASTYLVTDVTGIHS
PDQGSCLALVQALCPPAFVTGLPPHCALSVLAEIEHVDRGRVSGPTGWVDSLGNGQWVTDCRGGQIDATDPSLVHIFSGH
PVGHDDDADVLSAEAEHRIDVLRDLFLT
>Mature_428_residues
MRRCCPCGHLGYPATVIVDPGSARLCARTVSISDPGPLENYMTPNDSVCFLHRNYGMVGLGEVTRFETDSPAAADVWWEA
LCDDLEHETEMPDAEATGPVAFGSFTFDPDRSSSRSVMILPKTIIGRWPGYSWLTQLSWDSITDDSPHQQSVPRPPGAVT
VRDGVVSERSWRQVARLVQDIMSPGELDQIVLMRDVEAKASSSIDPRWIVETLRRQFPNAYTYLVEGSVGTTSKLTVGVS
KSLITSRVLTHSPATDANHQTLLTALQGKGPLAAYHDEIVGRTCARLEQFCDTVHVPDCPGCVYNDASTYLVTDVTGIHS
PDQGSCLALVQALCPPAFVTGLPPHCALSVLAEIEHVDRGRVSGPTGWVDSLGNGQWVTDCRGGQIDATDPSLVHIFSGH
PVGHDDDADVLSAEAEHRIDVLRDLFLT

Specific function: Siderophore biosynthesis; enterobactin biosynthesis. [C]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786809, Length=321, Percent_Identity=26.4797507788162, Blast_Score=79, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 46474; Mature: 46474

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRCCPCGHLGYPATVIVDPGSARLCARTVSISDPGPLENYMTPNDSVCFLHRNYGMVGL
CCCCCCCCCCCCCEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCEEEEECCCCEEEC
GEVTRFETDSPAAADVWWEALCDDLEHETEMPDAEATGPVAFGSFTFDPDRSSSRSVMIL
CCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCEEECCCCCCCCEEEEE
PKTIIGRWPGYSWLTQLSWDSITDDSPHQQSVPRPPGAVTVRDGVVSERSWRQVARLVQD
CHHHHCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHH
IMSPGELDQIVLMRDVEAKASSSIDPRWIVETLRRQFPNAYTYLVEGSVGTTSKLTVGVS
HCCCCCCCEEEEEECCCHHHCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEECCH
KSLITSRVLTHSPATDANHQTLLTALQGKGPLAAYHDEIVGRTCARLEQFCDTVHVPDCP
HHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GCVYNDASTYLVTDVTGIHSPDQGSCLALVQALCPPAFVTGLPPHCALSVLAEIEHVDRG
CCEECCCCCEEEEEECCCCCCCCCHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHCCC
RVSGPTGWVDSLGNGQWVTDCRGGQIDATDPSLVHIFSGHPVGHDDDADVLSAEAEHRID
CCCCCCCHHHCCCCCCEEECCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHH
VLRDLFLT
HHHHHHCC
>Mature Secondary Structure
MRRCCPCGHLGYPATVIVDPGSARLCARTVSISDPGPLENYMTPNDSVCFLHRNYGMVGL
CCCCCCCCCCCCCEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCEEEEECCCCEEEC
GEVTRFETDSPAAADVWWEALCDDLEHETEMPDAEATGPVAFGSFTFDPDRSSSRSVMIL
CCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCEEECCCCCCCCEEEEE
PKTIIGRWPGYSWLTQLSWDSITDDSPHQQSVPRPPGAVTVRDGVVSERSWRQVARLVQD
CHHHHCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHH
IMSPGELDQIVLMRDVEAKASSSIDPRWIVETLRRQFPNAYTYLVEGSVGTTSKLTVGVS
HCCCCCCCEEEEEECCCHHHCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEECCH
KSLITSRVLTHSPATDANHQTLLTALQGKGPLAAYHDEIVGRTCARLEQFCDTVHVPDCP
HHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GCVYNDASTYLVTDVTGIHSPDQGSCLALVQALCPPAFVTGLPPHCALSVLAEIEHVDRG
CCEECCCCCEEEEEECCCCCCCCCHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHCCC
RVSGPTGWVDSLGNGQWVTDCRGGQIDATDPSLVHIFSGHPVGHDDDADVLSAEAEHRID
CCCCCCCHHHCCCCCCEEECCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHH
VLRDLFLT
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629163; 8566759; 9387221; 9384377; 3131310 [H]