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The map label for this gene is mcl2 [H]

Identifier: 50843370

GI number: 50843370

Start: 2079066

End: 2079986

Strand: Reverse

Name: mcl2 [H]

Synonym: PPA1919

Alternate gene names: 50843370

Gene position: 2079986-2079066 (Counterclockwise)

Preceding gene: 50843371

Following gene: 50843364

Centisome position: 81.24

GC content: 63.74

Gene sequence:

>921_bases
ATGACTGGACTTCAGCCCGCTCTCGGCGCTGGATTCGGTTGCGGCACAATTGTGGGCATGGATTCTTTGCGCCCTCGCCG
TTCTGTTTTATTCATGCCCGGCGCCAATGCCAGGGCGCTGGAGAAGGCCCGCAGCATCGACTGCGACGGCATCATTATTG
ATTTAGAAGACGCGGTGGCCCCCGATCTCAAGGTTGAGGCCCGTGAGCGCGCGGCTGGCGTTGTGAAAGAGCATCCGTAC
GGCTACCGGGAGGTTGCCATCCGTGTCAACGGGATGGACACCCAGTGGTATCACGATGATCTCGCTGCCGCTGTTGCCGC
TGGCCCGGACGCTATCGCTGTCCCCAAGGTCGGCTCTGCCGAGCACGTGCACGCTATTGTCGCTGACATGGAGGCCGCCG
GGGCCGATGAGAGCGTCAAATTATGGGCCATGATCGAGACCCCACGGGCGGTGCTGGATTGCCTTGACATCGCGGAGGCC
TCCGACCGTCTGACGGTGCTCATCATGGGCACTAACGACCTGACTAAGGAGCTGCGCGCGTCTCACGTGGCTGGCCGCGC
CCCGGTGGTGACGGCGCTGCAGATGTGCATGCTGGCGGCTCGGGCAGCCGGTACCGCCATTCTGGATGGGGTCTACAACG
ACGTCCGCGATGTTGAGGGATTCACCTCCCAGTGCCGTGAGGCCCAGCTCATGGGATTCGACGGCAAGACGCTCATTCAC
CCCGGCCAGGTCGGCCCGGCCAACGAGGTCTTCGCCCCCGACGCTGAGGAGGTCGCCGAGGCTCGCGGCATCATCGACGC
ATGGGAGAACGGCAACGGTTCGGGCGTCGTGACCTACCAGGGCCGCATGATTGAGAACCTTCACGTCGATACTGCTCGTC
GAGCCCTGGCGATGGCTGAGGCGATCGAGGCACGAGGGTGA

Upstream 100 bases:

>100_bases
CTCGTGCCGACGCTGAGAAGCGTTTAGGGTGGGTGTTCGATACGTATCTACTGCGTCGTCGCTGAAGTCCAGTCATTGTG
ACCGAGTACTCACGGTCACA

Downstream 100 bases:

>100_bases
TGTGAGATCGTGGCCGTCAGCGGTGGTGCATGAGGGACGCCAGAACGGTGTTGCCATCTGTTGATGGCGGGATGAGGAAT
ACCGCGTGTGCCACGTAGAT

Product: citrate lyase beta chain

Products: acetate; oxaloacetate

Alternate protein names: (3S)-malyl-CoA thioesterase [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MTGLQPALGAGFGCGTIVGMDSLRPRRSVLFMPGANARALEKARSIDCDGIIIDLEDAVAPDLKVEARERAAGVVKEHPY
GYREVAIRVNGMDTQWYHDDLAAAVAAGPDAIAVPKVGSAEHVHAIVADMEAAGADESVKLWAMIETPRAVLDCLDIAEA
SDRLTVLIMGTNDLTKELRASHVAGRAPVVTALQMCMLAARAAGTAILDGVYNDVRDVEGFTSQCREAQLMGFDGKTLIH
PGQVGPANEVFAPDAEEVAEARGIIDAWENGNGSGVVTYQGRMIENLHVDTARRALAMAEAIEARG

Sequences:

>Translated_306_residues
MTGLQPALGAGFGCGTIVGMDSLRPRRSVLFMPGANARALEKARSIDCDGIIIDLEDAVAPDLKVEARERAAGVVKEHPY
GYREVAIRVNGMDTQWYHDDLAAAVAAGPDAIAVPKVGSAEHVHAIVADMEAAGADESVKLWAMIETPRAVLDCLDIAEA
SDRLTVLIMGTNDLTKELRASHVAGRAPVVTALQMCMLAARAAGTAILDGVYNDVRDVEGFTSQCREAQLMGFDGKTLIH
PGQVGPANEVFAPDAEEVAEARGIIDAWENGNGSGVVTYQGRMIENLHVDTARRALAMAEAIEARG
>Mature_305_residues
TGLQPALGAGFGCGTIVGMDSLRPRRSVLFMPGANARALEKARSIDCDGIIIDLEDAVAPDLKVEARERAAGVVKEHPYG
YREVAIRVNGMDTQWYHDDLAAAVAAGPDAIAVPKVGSAEHVHAIVADMEAAGADESVKLWAMIETPRAVLDCLDIAEAS
DRLTVLIMGTNDLTKELRASHVAGRAPVVTALQMCMLAARAAGTAILDGVYNDVRDVEGFTSQCREAQLMGFDGKTLIHP
GQVGPANEVFAPDAEEVAEARGIIDAWENGNGSGVVTYQGRMIENLHVDTARRALAMAEAIEARG

Specific function: Catalyzes the hydrolysis of (3S)-malyl-CoA to (3S)- malate and free CoA. Inactive towards beta-methylmalyl-CoA and other CoA esters [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=297, Percent_Identity=27.9461279461279, Blast_Score=130, Evalue=1e-30,
Organism=Escherichia coli, GI87081764, Length=286, Percent_Identity=34.965034965035, Blast_Score=144, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI17557458, Length=300, Percent_Identity=27.6666666666667, Blast_Score=115, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6

Molecular weight: Translated: 32385; Mature: 32254

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGLQPALGAGFGCGTIVGMDSLRPRRSVLFMPGANARALEKARSIDCDGIIIDLEDAVA
CCCCCCCCCCCCCCHHEECCCCCCCCCCEEEECCCCHHHHHHHHCCCCCEEEEECHHHCC
PDLKVEARERAAGVVKEHPYGYREVAIRVNGMDTQWYHDDLAAAVAAGPDAIAVPKVGSA
CCCEEEHHHHHHCHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCEECCCCCCH
EHVHAIVADMEAAGADESVKLWAMIETPRAVLDCLDIAEASDRLTVLIMGTNDLTKELRA
HHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHH
SHVAGRAPVVTALQMCMLAARAAGTAILDGVYNDVRDVEGFTSQCREAQLMGFDGKTLIH
HHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEC
PGQVGPANEVFAPDAEEVAEARGIIDAWENGNGSGVVTYQGRMIENLHVDTARRALAMAE
CCCCCCCCCCCCCCHHHHHHHCCCHHHCCCCCCCEEEEECCEEEECCCHHHHHHHHHHHH
AIEARG
HHHCCC
>Mature Secondary Structure 
TGLQPALGAGFGCGTIVGMDSLRPRRSVLFMPGANARALEKARSIDCDGIIIDLEDAVA
CCCCCCCCCCCCCHHEECCCCCCCCCCEEEECCCCHHHHHHHHCCCCCEEEEECHHHCC
PDLKVEARERAAGVVKEHPYGYREVAIRVNGMDTQWYHDDLAAAVAAGPDAIAVPKVGSA
CCCEEEHHHHHHCHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCEECCCCCCH
EHVHAIVADMEAAGADESVKLWAMIETPRAVLDCLDIAEASDRLTVLIMGTNDLTKELRA
HHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHH
SHVAGRAPVVTALQMCMLAARAAGTAILDGVYNDVRDVEGFTSQCREAQLMGFDGKTLIH
HHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEC
PGQVGPANEVFAPDAEEVAEARGIIDAWENGNGSGVVTYQGRMIENLHVDTARRALAMAE
CCCCCCCCCCCCCCHHHHHHHCCCHHHCCCCCCCEEEEECCEEEECCCHHHHHHHHHHHH
AIEARG
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0565 {oxaoacetate}} 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} [C]

Substrates: citrate

Specific reaction: citrate = acetate + oxaloacetate

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA