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The map label for this gene is nuoB2 [H]

Identifier: 50843355

GI number: 50843355

Start: 2062582

End: 2062980

Strand: Reverse

Name: nuoB2 [H]

Synonym: PPA1903

Alternate gene names: 50843355

Gene position: 2062980-2062582 (Counterclockwise)

Preceding gene: 50843362

Following gene: 50843354

Centisome position: 80.58

GC content: 64.66

Gene sequence:

>399_bases
GTGAAGTTCATCGACTGGTTCGCCGACGGATCCGTGTACGTCGTCGACCTCGCGCTGGCCTGCTGTGGGGTCGAGAGTGG
GGCTGCCGTGTTGGCCAACGCTCCGGTGCGCGAAGAGATCCCAGACGGCGCCCCGATAGTGGTGCTCGTTTCCGGGACGG
TGACAACTGGTCTGGCGCCCGTGGTGTCTGACACTGTCCATGAACTCAAGCGCAACCATCCCGTTGACGTGGTGGCTTTT
GGAGCGTGCGCCTGTGCAGGCGGACCGTACTGGGATTCAGCTGGTGTTGTCGCTGGACTTCCGGATGCGGGGGTCGACGC
CGATCACTGGATTCCGGGCTGTCCCCCCGGACCTGAAACGCTCACCGGGTTGTTGGCCTCGTCCCGGGAGGCCGCATGA

Upstream 100 bases:

>100_bases
GCTACTAACGCTACCATCGAGCCTTTGCTTATCGAACTTCGGTCCCGGCTGCGCGCTGCCACGCGAATGTGATGAACAGT
ATCCTCGGTAGGATGACCTG

Downstream 100 bases:

>100_bases
CGACCAGCGATGTCCCTGCAATCGGCTGGCACGACGCTGTTGCCCAGGCGGTGTCCACTCACCCGTGGCTGGCTCATCTG
ACGGCCATCGACGGGACAGG

Product: NADH dehydrogenase subunit

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B 2; NDH-1 subunit B 2 [H]

Number of amino acids: Translated: 132; Mature: 132

Protein sequence:

>132_residues
MKFIDWFADGSVYVVDLALACCGVESGAAVLANAPVREEIPDGAPIVVLVSGTVTTGLAPVVSDTVHELKRNHPVDVVAF
GACACAGGPYWDSAGVVAGLPDAGVDADHWIPGCPPGPETLTGLLASSREAA

Sequences:

>Translated_132_residues
MKFIDWFADGSVYVVDLALACCGVESGAAVLANAPVREEIPDGAPIVVLVSGTVTTGLAPVVSDTVHELKRNHPVDVVAF
GACACAGGPYWDSAGVVAGLPDAGVDADHWIPGCPPGPETLTGLLASSREAA
>Mature_132_residues
MKFIDWFADGSVYVVDLALACCGVESGAAVLANAPVREEIPDGAPIVVLVSGTVTTGLAPVVSDTVHELKRNHPVDVVAF
GACACAGGPYWDSAGVVAGLPDAGVDADHWIPGCPPGPETLTGLLASSREAA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translo

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Escherichia coli, GI1788624, Length=135, Percent_Identity=33.3333333333333, Blast_Score=63, Evalue=6e-12,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 13311; Mature: 13311

Theoretical pI: Translated: 4.00; Mature: 4.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFIDWFADGSVYVVDLALACCGVESGAAVLANAPVREEIPDGAPIVVLVSGTVTTGLAP
CCCEEEECCCCEEHHHHHHHHHCCCCCCEEEECCCCHHHCCCCCCEEEEEECCCCCCCHH
VVSDTVHELKRNHPVDVVAFGACACAGGPYWDSAGVVAGLPDAGVDADHWIPGCPPGPET
HHHHHHHHHHHCCCCEEEEECHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHH
LTGLLASSREAA
HHHHHHCCCCCC
>Mature Secondary Structure
MKFIDWFADGSVYVVDLALACCGVESGAAVLANAPVREEIPDGAPIVVLVSGTVTTGLAP
CCCEEEECCCCEEHHHHHHHHHCCCCCCEEEECCCCHHHCCCCCCEEEEEECCCCCCCHH
VVSDTVHELKRNHPVDVVAFGACACAGGPYWDSAGVVAGLPDAGVDADHWIPGCPPGPET
HHHHHHHHHHHCCCCEEEEECHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHH
LTGLLASSREAA
HHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11572948; 12692562 [H]