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The map label for this gene is rnhA [H]

Identifier: 50843189

GI number: 50843189

Start: 1885186

End: 1886088

Strand: Reverse

Name: rnhA [H]

Synonym: PPA1729

Alternate gene names: 50843189

Gene position: 1886088-1885186 (Counterclockwise)

Preceding gene: 50843190

Following gene: 161621793

Centisome position: 73.67

GC content: 63.57

Gene sequence:

>903_bases
ATGATAGTGGCCGCAGCTGACGGGTCCTCCCTCAACAATCCGGGGCCAGCCGGCTGGGCCTGGTATATCGACGACGACAC
CTGGGCTGCCGGCGGTTGGCCACGCGGTACGAATAACATGGGTGAGCTCAAGGCCGTTCTCGACGTGTTGCGCGCCACTC
GTGACGCCAACGAGCCACTGACGATCTTGTGTGACTCTCAGTACGCCATTAACTGCTGCACGAAGTGGATTCCGGGATGG
AAACGTCATGGATGGCGCAAACGTGACGGCAAGCCGGTACTCAACCGGGACCTCCTCGAGCCCATTGATGAAGAGCTCGA
CGGCCGTGACGTCACCTTTGAGTGGGTGAAAGGGCACGCTGGCCATGCTATGAACGAGGCCGCCGATCAGCGTGCTCGCG
CCGCCGCCACGGCCTTCCGCGACGGCACGCCGGTCGATCGCGGGCCTGGGTTCGGCGGGGTCGACCGAAGACCGCAGTCG
CAAGAAGATGTGCCGAGGCCCGGGAACGGTGATGAGCAAGAGCGGTTGTTTGATCTACCCGAGCGCCCTGTCACTCCCGA
CCAGGAGCTTATCGGCATCCTCACCGACAAAGAGCGCCGCTTCCTGGAGACCGATGCGATCGCCCACCGCAGCGCTTTGG
AGGAGCTTGCCGCCCCTGACTTCATCGAACATGACGGGAATGGCCGACTGTGGACGCGAGGTCGGGCTTTGGCCGACATG
CCGGTCCCCGAGGTGAGGGCCAGTATTGAGATTCTCGGAATGGTAAGGACTGGCCCACAGGACGTCCTGCTGCGGTGGCG
GGCACGGGCAGGATCTCGGGTCAGCGTGCGGGTTTCGCTATGGCACCGCGACCCAGATAGGGGCTGGCAGTTGAGGTTCC
ACCAAATCACCCCGGTGCGCTGA

Upstream 100 bases:

>100_bases
TCATCGACACCCCAGCCGGGGTGCAAACGATGACCGAGGTGCTACCGCAGGCCTTCGGCGTCGACAACCTTGATCGTGTC
GCCAACCAGCAGGAGAAGTC

Downstream 100 bases:

>100_bases
CAAGTTGCTCGGGTCATGAACCACGATGACCTCGATCGGTACATAGCATCGACCTACGCTGCTTCAATCGTTGACATCCT
CGATGTCGGCTACCGATAGA

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MIVAAADGSSLNNPGPAGWAWYIDDDTWAAGGWPRGTNNMGELKAVLDVLRATRDANEPLTILCDSQYAINCCTKWIPGW
KRHGWRKRDGKPVLNRDLLEPIDEELDGRDVTFEWVKGHAGHAMNEAADQRARAAATAFRDGTPVDRGPGFGGVDRRPQS
QEDVPRPGNGDEQERLFDLPERPVTPDQELIGILTDKERRFLETDAIAHRSALEELAAPDFIEHDGNGRLWTRGRALADM
PVPEVRASIEILGMVRTGPQDVLLRWRARAGSRVSVRVSLWHRDPDRGWQLRFHQITPVR

Sequences:

>Translated_300_residues
MIVAAADGSSLNNPGPAGWAWYIDDDTWAAGGWPRGTNNMGELKAVLDVLRATRDANEPLTILCDSQYAINCCTKWIPGW
KRHGWRKRDGKPVLNRDLLEPIDEELDGRDVTFEWVKGHAGHAMNEAADQRARAAATAFRDGTPVDRGPGFGGVDRRPQS
QEDVPRPGNGDEQERLFDLPERPVTPDQELIGILTDKERRFLETDAIAHRSALEELAAPDFIEHDGNGRLWTRGRALADM
PVPEVRASIEILGMVRTGPQDVLLRWRARAGSRVSVRVSLWHRDPDRGWQLRFHQITPVR
>Mature_300_residues
MIVAAADGSSLNNPGPAGWAWYIDDDTWAAGGWPRGTNNMGELKAVLDVLRATRDANEPLTILCDSQYAINCCTKWIPGW
KRHGWRKRDGKPVLNRDLLEPIDEELDGRDVTFEWVKGHAGHAMNEAADQRARAAATAFRDGTPVDRGPGFGGVDRRPQS
QEDVPRPGNGDEQERLFDLPERPVTPDQELIGILTDKERRFLETDAIAHRSALEELAAPDFIEHDGNGRLWTRGRALADM
PVPEVRASIEILGMVRTGPQDVLLRWRARAGSRVSVRVSLWHRDPDRGWQLRFHQITPVR

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain [H]

Homologues:

Organism=Homo sapiens, GI21359816, Length=108, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1786408, Length=134, Percent_Identity=43.2835820895522, Blast_Score=102, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI115532542, Length=146, Percent_Identity=37.6712328767123, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17505482, Length=128, Percent_Identity=33.59375, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45552509, Length=149, Percent_Identity=30.2013422818792, Blast_Score=78, Evalue=8e-15,
Organism=Drosophila melanogaster, GI17137476, Length=149, Percent_Identity=30.2013422818792, Blast_Score=78, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156 [H]

Pfam domain/function: PF00075 RnaseH [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 33652; Mature: 33652

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVAAADGSSLNNPGPAGWAWYIDDDTWAAGGWPRGTNNMGELKAVLDVLRATRDANEPL
CEEEECCCCCCCCCCCCCEEEEECCCCEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCE
TILCDSQYAINCCTKWIPGWKRHGWRKRDGKPVLNRDLLEPIDEELDGRDVTFEWVKGHA
EEEECCCHHHHHHHHHCCCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCC
GHAMNEAADQRARAAATAFRDGTPVDRGPGFGGVDRRPQSQEDVPRPGNGDEQERLFDLP
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCC
ERPVTPDQELIGILTDKERRFLETDAIAHRSALEELAAPDFIEHDGNGRLWTRGRALADM
CCCCCCHHHHEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCEEECCCEEECC
PVPEVRASIEILGMVRTGPQDVLLRWRARAGSRVSVRVSLWHRDPDRGWQLRFHQITPVR
CCHHHHHCEEEEEEECCCHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCEEEEEECCCCC
>Mature Secondary Structure
MIVAAADGSSLNNPGPAGWAWYIDDDTWAAGGWPRGTNNMGELKAVLDVLRATRDANEPL
CEEEECCCCCCCCCCCCCEEEEECCCCEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCE
TILCDSQYAINCCTKWIPGWKRHGWRKRDGKPVLNRDLLEPIDEELDGRDVTFEWVKGHA
EEEECCCHHHHHHHHHCCCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCC
GHAMNEAADQRARAAATAFRDGTPVDRGPGFGGVDRRPQSQEDVPRPGNGDEQERLFDLP
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCC
ERPVTPDQELIGILTDKERRFLETDAIAHRSALEELAAPDFIEHDGNGRLWTRGRALADM
CCCCCCHHHHEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCEEECCCEEECC
PVPEVRASIEILGMVRTGPQDVLLRWRARAGSRVSVRVSLWHRDPDRGWQLRFHQITPVR
CCHHHHHCEEEEEEECCCHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12606174 [H]