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The map label for this gene is nusA

Identifier: 50842972

GI number: 50842972

Start: 1613228

End: 1614199

Strand: Reverse

Name: nusA

Synonym: PPA1494

Alternate gene names: 50842972

Gene position: 1614199-1613228 (Counterclockwise)

Preceding gene: 50842973

Following gene: 50842971

Centisome position: 63.05

GC content: 59.26

Gene sequence:

>972_bases
ATGGACATCGACCTGTCAACTCTGCGCATGATCGAGCGGGACAAGGACATCCCGCTTGACTATCTGCTGACGACCCTAGA
GGACGCCCTCCTCAACGCATATGACAAGACTGAGGCTCCGGTTAACGGTGCCAAGGTTCAGCTGGACCGCAAGACTGGGA
ATGTCGCTGTCATGCTCCCGGAGAAGGACGAGGAGGGGCAGGTCGTCGGCTGGTATGACGGCACTCCCGAGGACTTCGGA
CGCGTAGCTGCATCAACTGCCCGGCAGGTCATTTTCCAACGGCTGCGCGAGGCCGAGGACGAGCAGAAATACGGTCATTT
TTCCGCCGTTGAGGGTGACGTCATCACCGGAGTCGTCCAGCAGTCTTACCGCGATACCCGGACCGTTCGCGTCGACCTGG
GCTCATTGGAGGGCATTATGCCTCCGGCGGAACAGGTTCCCGGGGAGAAATATTCCCATGGGCGTCGTATCCGTGTGTAC
GTCGTGGCCGTGCGCAAGGAAGCCCGTGGCCCGCAGGTCATTGTCTCGCGCACTCACCCGAACTTGGTGCGCAAGCTTTT
TGCTATGGAGGTACCCGAGATCGAGCAGGGGGTCGTCGAGATCAAGGCGCTGGCTCGTGAGGCCGGTCACCGCTCGAAGA
TTGCTGTCGTCTCGCATAATCCTGACGTTTCTGCCAAGGGAGCTTGCATTGGTCCGATGGGTCAGCGGGTACGTGCTGTG
ATGCACGAGTTGGATGAGGAAAAGATCGACATTATTGATTGGTCAGAGGATCCGGCCGAATTTGTGGGCGCTGCCCTGTC
TCCGTCCAAGGTGTCCTCGGTGACGGTCATTGACCCGAAAGCTAAGACCGCTCGGGCGATTGTTCCGGATTATCAGCTGT
CGCTAGCAATTGGCCGCGAGGGGCAGAACGCCCGACTGGCTGCTCGCCTGACGGGGTGGCGGATCGATATTCGTCCAGAT
AACAAGGTCTGA

Upstream 100 bases:

>100_bases
TACACCACTCGTGAGGTCCAGCTTGCTGACGTGGCGAAGGCCGTCGTCCAGGTGGAGCTCAATCGTCGCGACGCGCAGGC
GTCAAAGGAGGAGAACTGAG

Downstream 100 bases:

>100_bases
GACTGCCTGCTAGAGACACGACGACGCCCGGCCGTAGGGTCGGGCGTCATGCGTCTGTGGGTTGGTTTAAGGTGGGGCAG
TGACCACACGACCTCAGCGC

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MDIDLSTLRMIERDKDIPLDYLLTTLEDALLNAYDKTEAPVNGAKVQLDRKTGNVAVMLPEKDEEGQVVGWYDGTPEDFG
RVAASTARQVIFQRLREAEDEQKYGHFSAVEGDVITGVVQQSYRDTRTVRVDLGSLEGIMPPAEQVPGEKYSHGRRIRVY
VVAVRKEARGPQVIVSRTHPNLVRKLFAMEVPEIEQGVVEIKALAREAGHRSKIAVVSHNPDVSAKGACIGPMGQRVRAV
MHELDEEKIDIIDWSEDPAEFVGAALSPSKVSSVTVIDPKAKTARAIVPDYQLSLAIGREGQNARLAARLTGWRIDIRPD
NKV

Sequences:

>Translated_323_residues
MDIDLSTLRMIERDKDIPLDYLLTTLEDALLNAYDKTEAPVNGAKVQLDRKTGNVAVMLPEKDEEGQVVGWYDGTPEDFG
RVAASTARQVIFQRLREAEDEQKYGHFSAVEGDVITGVVQQSYRDTRTVRVDLGSLEGIMPPAEQVPGEKYSHGRRIRVY
VVAVRKEARGPQVIVSRTHPNLVRKLFAMEVPEIEQGVVEIKALAREAGHRSKIAVVSHNPDVSAKGACIGPMGQRVRAV
MHELDEEKIDIIDWSEDPAEFVGAALSPSKVSSVTVIDPKAKTARAIVPDYQLSLAIGREGQNARLAARLTGWRIDIRPD
NKV
>Mature_323_residues
MDIDLSTLRMIERDKDIPLDYLLTTLEDALLNAYDKTEAPVNGAKVQLDRKTGNVAVMLPEKDEEGQVVGWYDGTPEDFG
RVAASTARQVIFQRLREAEDEQKYGHFSAVEGDVITGVVQQSYRDTRTVRVDLGSLEGIMPPAEQVPGEKYSHGRRIRVY
VVAVRKEARGPQVIVSRTHPNLVRKLFAMEVPEIEQGVVEIKALAREAGHRSKIAVVSHNPDVSAKGACIGPMGQRVRAV
MHELDEEKIDIIDWSEDPAEFVGAALSPSKVSSVTVIDPKAKTARAIVPDYQLSLAIGREGQNARLAARLTGWRIDIRPD
NKV

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=336, Percent_Identity=36.9047619047619, Blast_Score=200, Evalue=1e-52,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N [H]

EC number: NA

Molecular weight: Translated: 35739; Mature: 35739

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIDLSTLRMIERDKDIPLDYLLTTLEDALLNAYDKTEAPVNGAKVQLDRKTGNVAVMLP
CCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCEEEEEC
EKDEEGQVVGWYDGTPEDFGRVAASTARQVIFQRLREAEDEQKYGHFSAVEGDVITGVVQ
CCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCHHHHHHHH
QSYRDTRTVRVDLGSLEGIMPPAEQVPGEKYSHGRRIRVYVVAVRKEARGPQVIVSRTHP
HHHCCCEEEEEECCCCCCCCCCHHHCCCCCCCCCCEEEEEEEEEECCCCCCEEEEECCCH
NLVRKLFAMEVPEIEQGVVEIKALAREAGHRSKIAVVSHNPDVSAKGACIGPMGQRVRAV
HHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEECHHHHHHHHH
MHELDEEKIDIIDWSEDPAEFVGAALSPSKVSSVTVIDPKAKTARAIVPDYQLSLAIGRE
HHHCCCCCEEEEECCCCHHHHHCCCCCCCCCCEEEEECCCCCCCEEECCCCEEEEEECCC
GQNARLAARLTGWRIDIRPDNKV
CCCCEEEEEEECEEEEECCCCCC
>Mature Secondary Structure
MDIDLSTLRMIERDKDIPLDYLLTTLEDALLNAYDKTEAPVNGAKVQLDRKTGNVAVMLP
CCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCEEEEEC
EKDEEGQVVGWYDGTPEDFGRVAASTARQVIFQRLREAEDEQKYGHFSAVEGDVITGVVQ
CCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCHHHHHHHH
QSYRDTRTVRVDLGSLEGIMPPAEQVPGEKYSHGRRIRVYVVAVRKEARGPQVIVSRTHP
HHHCCCEEEEEECCCCCCCCCCHHHCCCCCCCCCCEEEEEEEEEECCCCCCEEEEECCCH
NLVRKLFAMEVPEIEQGVVEIKALAREAGHRSKIAVVSHNPDVSAKGACIGPMGQRVRAV
HHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEECHHHHHHHHH
MHELDEEKIDIIDWSEDPAEFVGAALSPSKVSSVTVIDPKAKTARAIVPDYQLSLAIGRE
HHHCCCCCEEEEECCCCHHHHHCCCCCCCCCCEEEEECCCCCCCEEECCCCEEEEEECCC
GQNARLAARLTGWRIDIRPDNKV
CCCCEEEEEEECEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]