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The map label for this gene is bioB [H]

Identifier: 50842889

GI number: 50842889

Start: 1528593

End: 1529759

Strand: Direct

Name: bioB [H]

Synonym: PPA1407

Alternate gene names: 50842889

Gene position: 1528593-1529759 (Clockwise)

Preceding gene: 50842865

Following gene: 50842897

Centisome position: 59.7

GC content: 60.15

Gene sequence:

>1167_bases
ATGGACTACATTGAATCCCGTTCAATCCTGAACGGCGTTCAGGACGTCTCCAGTCTCGGAAGGACCAGAGTATTGCTGAA
TCTAGCCGACATGACCGAACGCGGCCTGAGGGGGGAGTCCATTACCCGCGAGGAGGCCCTCGAGATTCTTCGCAGCAGTG
ATGATGAGCTCATGTCAATCATCGCCGCCGCCGGAAAAGTGCGTCGCCACTTTTTCGATAACCGGGTTCGCCTCAACTAC
CTGGTCAACCTCAAGTCCGGCCTGTGTCCCGAAGACTGCTCCTATTGCTCGCAGCGTCTGGGATCGCGTGCCGAGATCAT
GAAATACTCCTGGGCCGATCCGCAGAAGGTACACGACGCCGTCGAGGCTGGGATTGCCGGTGGTGCACGTCGCGTCTGCA
TGGTGGCCTCCGGTCATGGTCCATCTCGGCGAGACGTCGAGCGCGTCAATGGCATGGTACGTTCCCTCAAAGCCGACCAC
CCCGACGTTGAGGTTTGTGTCTGTTTGGGGTTTGTCGACGACGAGAAGGCCGCTTCCATCAAGGAGGCGGGCGCCGACGC
CTACAACCACAATGCCAATACCGCTCGCTCTCATTACGGCAAGATCTGCAGTACCCACTCCTACGAGGACAGGATGGATA
CCGTCGAAGTCCTCAAGCGCAATGGTCTGTCGCCCTGCTCCGGAGTTATTGCCGGCATGGGCGAGACCGACGAGGAATTC
GTCGATGTTATCTTCGACCTGCGCAAGCATGGGGTCGATTCGGTGCCGGTGAATTTCTTGCTCCCCTTCGAGGGCACTCC
GCTGGCCGGTGGAGCCCAGCACATCACCCCACAGTGGTGCCTCAAGCGTCTAGCCATGGTGCGCTTTGCTCACCCCGACT
CCGAGGTGCGCGCCGCTGCCGGGCGTGAGCAACACATCCGTACCATGCAGCCGCTAGCCCTTGAGGTCGTCAACTCGATC
TTCCTGGGGGACTACCTCACCAGTGAAGGGGCTGCCGGCGCCGCCGACCTGCAGATGATCGAGGATGGTGGATTCATTCC
GGAGGGGGCCGACGGTCAACCGATGGTTCACACCGACGTCAATTCGCATCATTCAGCAAACCTGCCCGTCAACGCGGTCC
CGATCCGTCATCGCGGCATCGGAACCGAAGTTCCGGCGAACGCCTAA

Upstream 100 bases:

>100_bases
TCTCTACTCCTGCCCGAACAACGTCCGGCTTCGTCAGGCTCACGATGACTCCCTTGACGACGACACCATTTCCGGGGGTA
GTCCACATTGGTGCTGCCTC

Downstream 100 bases:

>100_bases
CTACTCGAGTCACATCGTGGGGTGGAAGCGATGGCGTCGTCTCCACCCCACATACATCAGTTGTGCTGGGAGTCCGCCGT
CGTCACAGACTCCGGCGGCG

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MDYIESRSILNGVQDVSSLGRTRVLLNLADMTERGLRGESITREEALEILRSSDDELMSIIAAAGKVRRHFFDNRVRLNY
LVNLKSGLCPEDCSYCSQRLGSRAEIMKYSWADPQKVHDAVEAGIAGGARRVCMVASGHGPSRRDVERVNGMVRSLKADH
PDVEVCVCLGFVDDEKAASIKEAGADAYNHNANTARSHYGKICSTHSYEDRMDTVEVLKRNGLSPCSGVIAGMGETDEEF
VDVIFDLRKHGVDSVPVNFLLPFEGTPLAGGAQHITPQWCLKRLAMVRFAHPDSEVRAAAGREQHIRTMQPLALEVVNSI
FLGDYLTSEGAAGAADLQMIEDGGFIPEGADGQPMVHTDVNSHHSANLPVNAVPIRHRGIGTEVPANA

Sequences:

>Translated_388_residues
MDYIESRSILNGVQDVSSLGRTRVLLNLADMTERGLRGESITREEALEILRSSDDELMSIIAAAGKVRRHFFDNRVRLNY
LVNLKSGLCPEDCSYCSQRLGSRAEIMKYSWADPQKVHDAVEAGIAGGARRVCMVASGHGPSRRDVERVNGMVRSLKADH
PDVEVCVCLGFVDDEKAASIKEAGADAYNHNANTARSHYGKICSTHSYEDRMDTVEVLKRNGLSPCSGVIAGMGETDEEF
VDVIFDLRKHGVDSVPVNFLLPFEGTPLAGGAQHITPQWCLKRLAMVRFAHPDSEVRAAAGREQHIRTMQPLALEVVNSI
FLGDYLTSEGAAGAADLQMIEDGGFIPEGADGQPMVHTDVNSHHSANLPVNAVPIRHRGIGTEVPANA
>Mature_388_residues
MDYIESRSILNGVQDVSSLGRTRVLLNLADMTERGLRGESITREEALEILRSSDDELMSIIAAAGKVRRHFFDNRVRLNY
LVNLKSGLCPEDCSYCSQRLGSRAEIMKYSWADPQKVHDAVEAGIAGGARRVCMVASGHGPSRRDVERVNGMVRSLKADH
PDVEVCVCLGFVDDEKAASIKEAGADAYNHNANTARSHYGKICSTHSYEDRMDTVEVLKRNGLSPCSGVIAGMGETDEEF
VDVIFDLRKHGVDSVPVNFLLPFEGTPLAGGAQHITPQWCLKRLAMVRFAHPDSEVRAAAGREQHIRTMQPLALEVVNSI
FLGDYLTSEGAAGAADLQMIEDGGFIPEGADGQPMVHTDVNSHHSANLPVNAVPIRHRGIGTEVPANA

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=297, Percent_Identity=33.3333333333333, Blast_Score=166, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6321725, Length=349, Percent_Identity=30.945558739255, Blast_Score=151, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 42258; Mature: 42258

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYIESRSILNGVQDVSSLGRTRVLLNLADMTERGLRGESITREEALEILRSSDDELMSI
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHH
IAAAGKVRRHFFDNRVRLNYLVNLKSGLCPEDCSYCSQRLGSRAEIMKYSWADPQKVHDA
HHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHH
VEAGIAGGARRVCMVASGHGPSRRDVERVNGMVRSLKADHPDVEVCVCLGFVDDEKAASI
HHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHH
KEAGADAYNHNANTARSHYGKICSTHSYEDRMDTVEVLKRNGLSPCSGVIAGMGETDEEF
HHHCCCHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHH
VDVIFDLRKHGVDSVPVNFLLPFEGTPLAGGAQHITPQWCLKRLAMVRFAHPDSEVRAAA
HHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHC
GREQHIRTMQPLALEVVNSIFLGDYLTSEGAAGAADLQMIEDGGFIPEGADGQPMVHTDV
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEECCCCCCCCCCCCCEEEECC
NSHHSANLPVNAVPIRHRGIGTEVPANA
CCCCCCCCCCEEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MDYIESRSILNGVQDVSSLGRTRVLLNLADMTERGLRGESITREEALEILRSSDDELMSI
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHH
IAAAGKVRRHFFDNRVRLNYLVNLKSGLCPEDCSYCSQRLGSRAEIMKYSWADPQKVHDA
HHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHH
VEAGIAGGARRVCMVASGHGPSRRDVERVNGMVRSLKADHPDVEVCVCLGFVDDEKAASI
HHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHH
KEAGADAYNHNANTARSHYGKICSTHSYEDRMDTVEVLKRNGLSPCSGVIAGMGETDEEF
HHHCCCHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHH
VDVIFDLRKHGVDSVPVNFLLPFEGTPLAGGAQHITPQWCLKRLAMVRFAHPDSEVRAAA
HHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHC
GREQHIRTMQPLALEVVNSIFLGDYLTSEGAAGAADLQMIEDGGFIPEGADGQPMVHTDV
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEECCCCCCCCCCCCCEEEECC
NSHHSANLPVNAVPIRHRGIGTEVPANA
CCCCCCCCCCEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA