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The map label for this gene is gluQ

Identifier: 50842865

GI number: 50842865

Start: 1503535

End: 1504578

Strand: Direct

Name: gluQ

Synonym: PPA1383

Alternate gene names: 50842865

Gene position: 1503535-1504578 (Clockwise)

Preceding gene: 50842853

Following gene: 50842889

Centisome position: 58.73

GC content: 63.51

Gene sequence:

>1044_bases
ATGCCCGCCGCGTCACCCCAATTGTTGCCTACACTGCCGCTCGTGACCGATTTCGCCACCACTCACTCCGGTCTTCGACA
CGCAGCTGGCCGTTTCGCCCCCTCCCCAACCTCAGCACTGCATCTGGGGAACCTCCGCACCGCATTAGCGGCCTGGCTTT
TGGCACGCTCGACAGGTCGTCGGTTCGTGGTACGCATCGAGGATTTAGATCGTGCGCGGGTGGCCGCGGCCGGAAAAATT
GCCTCGACTCAACTACGCGACCTGGAGTCCCTAGGTCTGGATTGGGATGGTCCAGTCGTCCGACAGTCTGAGCGCCTCGA
CTTGTACGCCGACGCAGTAGCGGGTCTGGAAACCTACCCCTGTTTTTGCACTCGCAGGGAAATCGCTGCGGCCACTACCG
CCCCCAACGAGGCCGATTGGCGCCCGTATCCGGGTACCTGCCGCCGCCTCACCGTCCAGCAGAGACACGAACGTCTGACC
ACCCGGGCGCCAGCCACCCGACTGGCCAGTCAACTGAACACTTTCGAAGCGACCGACCTCGCTCGCGGCTGTGCTCGAGG
ACGGGTGGACGATCTTGTCCTGGTGCGCAATGACGGCACGCCGGCCTACAACCTTGCGGTAGTCGTCGACGACGGTCTGC
AAGGGGTCGATCAGGTCGTTCGAGCCCGAGACCTATGGTCCTCGGCCCCTCGCCAAGCCCAGTTGGCCGAACTGCTCGGA
TTTATCCCTCCGGTCTACGCCCACACCGGTCTTGTCACCGGTCCAGGAGGGGTACGCTTGTCAAAGTCGGCGGGTGCGGC
GGGGCTGTCCGATCTCGTGGATTCAGGTATTGACCACCGCCAGGTCGTGGCGTGGCTCTGCCGTTCCCTCGGACTCCCTG
AAGTTGCTGATCCCCATGAGCTCGTCAACAATGTGGCCCTGACACCGCCTACCCCTGTTGCGGGCATTCACCGCTTAGAT
CCTAGGCCTCTTGGCGGAGCGATGGGGTGGTGGAGTGATGCGGTTCTCGATGTGGCGGTGCTGCGCGAGACCCGTCGACC
CTAA

Upstream 100 bases:

>100_bases
TGACCAGGAGAGCGATGACGAGGTTAACCAATCTCTCCGAACGCTGTGCAGCCATCGGCACCCTCCTTCCAGCGTCCCAG
CCTACCCATCTGACGGGGGC

Downstream 100 bases:

>100_bases
GACACATATCGGCCGACATGTCTCGCGCAAAGCCATCGCCACTCAGGATTCTTTTTGAGCCGAAAGGATGTCGATAACGT
AGATCAGGGTCTCGCCCTTC

Product: glutamyl-tRNA synthetase

Products: AMP; diphosphate; L-glutamyl-tRNA(Glu)

Alternate protein names: Glu-Q-RSs

Number of amino acids: Translated: 347; Mature: 346

Protein sequence:

>347_residues
MPAASPQLLPTLPLVTDFATTHSGLRHAAGRFAPSPTSALHLGNLRTALAAWLLARSTGRRFVVRIEDLDRARVAAAGKI
ASTQLRDLESLGLDWDGPVVRQSERLDLYADAVAGLETYPCFCTRREIAAATTAPNEADWRPYPGTCRRLTVQQRHERLT
TRAPATRLASQLNTFEATDLARGCARGRVDDLVLVRNDGTPAYNLAVVVDDGLQGVDQVVRARDLWSSAPRQAQLAELLG
FIPPVYAHTGLVTGPGGVRLSKSAGAAGLSDLVDSGIDHRQVVAWLCRSLGLPEVADPHELVNNVALTPPTPVAGIHRLD
PRPLGGAMGWWSDAVLDVAVLRETRRP

Sequences:

>Translated_347_residues
MPAASPQLLPTLPLVTDFATTHSGLRHAAGRFAPSPTSALHLGNLRTALAAWLLARSTGRRFVVRIEDLDRARVAAAGKI
ASTQLRDLESLGLDWDGPVVRQSERLDLYADAVAGLETYPCFCTRREIAAATTAPNEADWRPYPGTCRRLTVQQRHERLT
TRAPATRLASQLNTFEATDLARGCARGRVDDLVLVRNDGTPAYNLAVVVDDGLQGVDQVVRARDLWSSAPRQAQLAELLG
FIPPVYAHTGLVTGPGGVRLSKSAGAAGLSDLVDSGIDHRQVVAWLCRSLGLPEVADPHELVNNVALTPPTPVAGIHRLD
PRPLGGAMGWWSDAVLDVAVLRETRRP
>Mature_346_residues
PAASPQLLPTLPLVTDFATTHSGLRHAAGRFAPSPTSALHLGNLRTALAAWLLARSTGRRFVVRIEDLDRARVAAAGKIA
STQLRDLESLGLDWDGPVVRQSERLDLYADAVAGLETYPCFCTRREIAAATTAPNEADWRPYPGTCRRLTVQQRHERLTT
RAPATRLASQLNTFEATDLARGCARGRVDDLVLVRNDGTPAYNLAVVVDDGLQGVDQVVRARDLWSSAPRQAQLAELLGF
IPPVYAHTGLVTGPGGVRLSKSAGAAGLSDLVDSGIDHRQVVAWLCRSLGLPEVADPHELVNNVALTPPTPVAGIHRLDP
RPLGGAMGWWSDAVLDVAVLRETRRP

Specific function: Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5- dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble positi

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily

Homologues:

Organism=Homo sapiens, GI134288884, Length=267, Percent_Identity=35.2059925093633, Blast_Score=128, Evalue=8e-30,
Organism=Escherichia coli, GI226510925, Length=270, Percent_Identity=38.8888888888889, Blast_Score=148, Evalue=5e-37,
Organism=Escherichia coli, GI1788743, Length=303, Percent_Identity=32.013201320132, Blast_Score=144, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI71993394, Length=262, Percent_Identity=30.9160305343511, Blast_Score=109, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324540, Length=267, Percent_Identity=34.4569288389513, Blast_Score=138, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24665321, Length=292, Percent_Identity=33.2191780821918, Blast_Score=124, Evalue=7e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLUQ_PROAC (Q6A7Y1)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_056092.1
- ProteinModelPortal:   Q6A7Y1
- SMR:   Q6A7Y1
- GeneID:   2931565
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA1383
- NMPDR:   fig|267747.1.peg.1369
- HOGENOM:   HBG628189
- OMA:   DAPSSYH
- ProtClustDB:   CLSK2763938
- BioCyc:   PACN267747:PPA1383-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01428
- InterPro:   IPR022380
- InterPro:   IPR000924
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10119
- PRINTS:   PR00987

Pfam domain/function: PF00749 tRNA-synt_1c

EC number: 6.1.1.17

Molecular weight: Translated: 37357; Mature: 37226

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS00136 SUBTILASE_ASP

Important sites: BINDING 67-67 BINDING 203-203 BINDING 221-221 BINDING 262-262

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAASPQLLPTLPLVTDFATTHSGLRHAAGRFAPSPTSALHLGNLRTALAAWLLARSTGR
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCC
RFVVRIEDLDRARVAAAGKIASTQLRDLESLGLDWDGPVVRQSERLDLYADAVAGLETYP
EEEEEECCCCHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCC
CFCTRREIAAATTAPNEADWRPYPGTCRRLTVQQRHERLTTRAPATRLASQLNTFEATDL
CHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
ARGCARGRVDDLVLVRNDGTPAYNLAVVVDDGLQGVDQVVRARDLWSSAPRQAQLAELLG
HHHHHCCCCCCEEEEECCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHH
FIPPVYAHTGLVTGPGGVRLSKSAGAAGLSDLVDSGIDHRQVVAWLCRSLGLPEVADPHE
HCCHHHHHCCEEECCCCCEEECCCCCCCHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHH
LVNNVALTPPTPVAGIHRLDPRPLGGAMGWWSDAVLDVAVLRETRRP
HHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PAASPQLLPTLPLVTDFATTHSGLRHAAGRFAPSPTSALHLGNLRTALAAWLLARSTGR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCC
RFVVRIEDLDRARVAAAGKIASTQLRDLESLGLDWDGPVVRQSERLDLYADAVAGLETYP
EEEEEECCCCHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCC
CFCTRREIAAATTAPNEADWRPYPGTCRRLTVQQRHERLTTRAPATRLASQLNTFEATDL
CHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
ARGCARGRVDDLVLVRNDGTPAYNLAVVVDDGLQGVDQVVRARDLWSSAPRQAQLAELLG
HHHHHCCCCCCEEEEECCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHH
FIPPVYAHTGLVTGPGGVRLSKSAGAAGLSDLVDSGIDHRQVVAWLCRSLGLPEVADPHE
HCCHHHHHCCEEECCCCCEEECCCCCCCHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHH
LVNNVALTPPTPVAGIHRLDPRPLGGAMGWWSDAVLDVAVLRETRRP
HHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; tRNA(Glu)

Specific reaction: ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA