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The map label for this gene is ppnK

Identifier: 50842877

GI number: 50842877

Start: 1513522

End: 1514478

Strand: Reverse

Name: ppnK

Synonym: PPA1395

Alternate gene names: 50842877

Gene position: 1514478-1513522 (Counterclockwise)

Preceding gene: 50842878

Following gene: 50842876

Centisome position: 59.15

GC content: 60.82

Gene sequence:

>957_bases
ATGGGCCGCTACTGTGGTCTTGTGAACGACACGAGCCCTTCGAGATACGTTGTCGTCGTCACCCATGCCACGCGGGACGA
CGCTTTTGACGCGGCTGCCGAATTCATCTCTGAAATGGCGGGGCGAGACATTGGTTGCGCGGTTCCGGATGATCAGGCGA
AGCCGATGTCAAGCAAGCTGCCAGGGATCGATCTTGAAAGCTTGGGAGAGTTCGCCCACGAGGCGGAGGTGGTCGTCGTC
TTTGGCGGCGACGGCACGATCTTGCGAGCTGCTGAATGGTCATTACCTCGCCACGTTCCCATGATTGGCGTCAACCTTGG
CCATGTCGGTTTTCTGGCTGAGCTGGAGCGCTCCGATATGGCGGATCTAGTGAACAAGGTGTGTTCGCGCGACTACACCG
TTGAGGATCGCCTCGTGCTTAAAACCACCGTCACCGAGCATTCCGGACAACACCGTTGGAGTTCTTTCGCCGTCAACGAG
TTGTCTCTGGAAAAGGCAGCCCGGCGGCGCATGCTCGACGTTCTGGCGTCTGTCGACGAGTTGCCGGTGCAACGCTGGAG
TTGCGACGGGATCCTGGTCTCGACCCCGACCGGATCGACGGCTTACGCGTTCTCAGCTGGCGGCCCGGTCATGTGGCCCG
ATCTCGATGCCATGCTCATGGTGCCGTTGAGCGCTCACGCTCTCTTTGCTCGGCCGCTGGTCATGAGTCCAGCTGCTCGA
GTGGACCTTGACATCCAGCCAGACGGTTCAGAATCGGCGGTTCTGTGGTGCGACGGGCGCCGATCGTGCACCGTACGACC
GGGGGAAAGAATCACCGTCGTCCGCCATCCCGACCGTCTGCGCATTGCTCGTCTGGCCGCGCAGCCCTTCACATCGCGTC
TGGTCAAGAAGTTTGAGCTCCCGGTCAGCGGGTGGCGTCAGGGTCGTGACCGTCATCACCTAGAGGAGACTTCGTGA

Upstream 100 bases:

>100_bases
GCGTGACGAGTGCGATAGCCCGTTGCCCGGGCAGGATGGAAACGTTGAGCACTTCGTCTTGCTGGAACGTACGGGTCGGT
GACAGACGTCCGGGCATATC

Downstream 100 bases:

>100_bases
TACGTAGTGTGCGAATTCGTGGACTCGGCGTCATCGATGAGACGGTCCTCGAACCCTCGTCCGCGCTGACGGCAGTCACC
GGCGAGACCGGCGCCGGAAA

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MGRYCGLVNDTSPSRYVVVVTHATRDDAFDAAAEFISEMAGRDIGCAVPDDQAKPMSSKLPGIDLESLGEFAHEAEVVVV
FGGDGTILRAAEWSLPRHVPMIGVNLGHVGFLAELERSDMADLVNKVCSRDYTVEDRLVLKTTVTEHSGQHRWSSFAVNE
LSLEKAARRRMLDVLASVDELPVQRWSCDGILVSTPTGSTAYAFSAGGPVMWPDLDAMLMVPLSAHALFARPLVMSPAAR
VDLDIQPDGSESAVLWCDGRRSCTVRPGERITVVRHPDRLRIARLAAQPFTSRLVKKFELPVSGWRQGRDRHHLEETS

Sequences:

>Translated_318_residues
MGRYCGLVNDTSPSRYVVVVTHATRDDAFDAAAEFISEMAGRDIGCAVPDDQAKPMSSKLPGIDLESLGEFAHEAEVVVV
FGGDGTILRAAEWSLPRHVPMIGVNLGHVGFLAELERSDMADLVNKVCSRDYTVEDRLVLKTTVTEHSGQHRWSSFAVNE
LSLEKAARRRMLDVLASVDELPVQRWSCDGILVSTPTGSTAYAFSAGGPVMWPDLDAMLMVPLSAHALFARPLVMSPAAR
VDLDIQPDGSESAVLWCDGRRSCTVRPGERITVVRHPDRLRIARLAAQPFTSRLVKKFELPVSGWRQGRDRHHLEETS
>Mature_317_residues
GRYCGLVNDTSPSRYVVVVTHATRDDAFDAAAEFISEMAGRDIGCAVPDDQAKPMSSKLPGIDLESLGEFAHEAEVVVVF
GGDGTILRAAEWSLPRHVPMIGVNLGHVGFLAELERSDMADLVNKVCSRDYTVEDRLVLKTTVTEHSGQHRWSSFAVNEL
SLEKAARRRMLDVLASVDELPVQRWSCDGILVSTPTGSTAYAFSAGGPVMWPDLDAMLMVPLSAHALFARPLVMSPAARV
DLDIQPDGSESAVLWCDGRRSCTVRPGERITVVRHPDRLRIARLAAQPFTSRLVKKFELPVSGWRQGRDRHHLEETS

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Homo sapiens, GI55743112, Length=233, Percent_Identity=25.7510729613734, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1788968, Length=263, Percent_Identity=30.4182509505703, Blast_Score=130, Evalue=8e-32,
Organism=Saccharomyces cerevisiae, GI6325068, Length=212, Percent_Identity=25.9433962264151, Blast_Score=84, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6322509, Length=221, Percent_Identity=26.6968325791855, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320794, Length=246, Percent_Identity=22.7642276422764, Blast_Score=79, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK_PROAC (Q6A7W9)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_056104.1
- ProteinModelPortal:   Q6A7W9
- SMR:   Q6A7W9
- GeneID:   2931825
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA1395
- NMPDR:   fig|267747.1.peg.1381
- HOGENOM:   HBG713904
- OMA:   CVPEGFD
- ProtClustDB:   PRK03372
- BioCyc:   PACN267747:PPA1395-MONOMER
- BRENDA:   2.7.1.23
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 34945; Mature: 34814

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRYCGLVNDTSPSRYVVVVTHATRDDAFDAAAEFISEMAGRDIGCAVPDDQAKPMSSKL
CCCEECCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCC
PGIDLESLGEFAHEAEVVVVFGGDGTILRAAEWSLPRHVPMIGVNLGHVGFLAELERSDM
CCCCHHHHHHHHCCCEEEEEECCCCCEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHH
ADLVNKVCSRDYTVEDRLVLKTTVTEHSGQHRWSSFAVNELSLEKAARRRMLDVLASVDE
HHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPVQRWSCDGILVSTPTGSTAYAFSAGGPVMWPDLDAMLMVPLSAHALFARPLVMSPAAR
CCCHHCCCCEEEEECCCCCEEEEEECCCCEECCCCCEEEEECHHHHHHHHCHHHCCCCHH
VDLDIQPDGSESAVLWCDGRRSCTVRPGERITVVRHPDRLRIARLAAQPFTSRLVKKFEL
EEEEECCCCCCCEEEEECCCCEEEECCCCEEEEEECCCHHHHHHHHHCHHHHHHHHHHCC
PVSGWRQGRDRHHLEETS
CHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
GRYCGLVNDTSPSRYVVVVTHATRDDAFDAAAEFISEMAGRDIGCAVPDDQAKPMSSKL
CCEECCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCC
PGIDLESLGEFAHEAEVVVVFGGDGTILRAAEWSLPRHVPMIGVNLGHVGFLAELERSDM
CCCCHHHHHHHHCCCEEEEEECCCCCEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHH
ADLVNKVCSRDYTVEDRLVLKTTVTEHSGQHRWSSFAVNELSLEKAARRRMLDVLASVDE
HHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPVQRWSCDGILVSTPTGSTAYAFSAGGPVMWPDLDAMLMVPLSAHALFARPLVMSPAAR
CCCHHCCCCEEEEECCCCCEEEEEECCCCEECCCCCEEEEECHHHHHHHHCHHHCCCCHH
VDLDIQPDGSESAVLWCDGRRSCTVRPGERITVVRHPDRLRIARLAAQPFTSRLVKKFEL
EEEEECCCCCCCEEEEECCCCEEEECCCCEEEEEECCCHHHHHHHHHCHHHHHHHHHHCC
PVSGWRQGRDRHHLEETS
CHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA