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The map label for this gene is leuC [H]

Identifier: 50842847

GI number: 50842847

Start: 1486204

End: 1486539

Strand: Reverse

Name: leuC [H]

Synonym: PPA1363

Alternate gene names: 50842847

Gene position: 1486539-1486204 (Counterclockwise)

Preceding gene: 50842849

Following gene: 50842846

Centisome position: 58.06

GC content: 60.71

Gene sequence:

>336_bases
ATGAGCTTATTAGTTGGGCGCTCGGTTCGTCGTTCGGTTCTGACCATTGCCACCGAGGACCACAACATCCCTACCGTCGG
CAGTATCGAGCCAATTGTTGACCCGGTCTCGCGCGCTCAGGTCGAGGCGTTGCGAGCCAACGCACCTGAGTTCGGTGTGC
CGCCTTTAGGGGACGCCGATCAGGGGGTCGTGCACATCATCGGTCCCCAACTGGGTCTCACCCAGCCGGGGATGACTGTC
GTCTGTGGTGACTCCCACACCTCTACACACGGCGCCTTCGGGGCCTTAGCCTTCGGTATCGGTACCAGCGAGGTTGAGCA
TGTGTTGGCTACCTAG

Upstream 100 bases:

>100_bases
GGACGCTCACATCGTTCAGGCATGGGGCACGACGGGGAGCGTGATCTTCTCTACATCGACCTTCACCTCGTCCACGAGGT
GACAAGTCCGCAGGCTTTCG

Downstream 100 bases:

>100_bases
ACCCTCCCGCAGGTTCGTCCCACAACGATGGCCGTCATGGTGGATGGGGAAGTGCCTGAGGAGGTCACACCTAAGGACCT
CATCCTGGCCCTCATCTCCG

Product: 3-isopropylmalate dehydratase large subunit fragment

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 111; Mature: 110

Protein sequence:

>111_residues
MSLLVGRSVRRSVLTIATEDHNIPTVGSIEPIVDPVSRAQVEALRANAPEFGVPPLGDADQGVVHIIGPQLGLTQPGMTV
VCGDSHTSTHGAFGALAFGIGTSEVEHVLAT

Sequences:

>Translated_111_residues
MSLLVGRSVRRSVLTIATEDHNIPTVGSIEPIVDPVSRAQVEALRANAPEFGVPPLGDADQGVVHIIGPQLGLTQPGMTV
VCGDSHTSTHGAFGALAFGIGTSEVEHVLAT
>Mature_110_residues
SLLVGRSVRRSVLTIATEDHNIPTVGSIEPIVDPVSRAQVEALRANAPEFGVPPLGDADQGVVHIIGPQLGLTQPGMTVV
CGDSHTSTHGAFGALAFGIGTSEVEHVLAT

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786259, Length=108, Percent_Identity=57.4074074074074, Blast_Score=117, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6321429, Length=114, Percent_Identity=62.280701754386, Blast_Score=132, Evalue=1e-32,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 11417; Mature: 11286

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLVGRSVRRSVLTIATEDHNIPTVGSIEPIVDPVSRAQVEALRANAPEFGVPPLGDAD
CCCCCCCHHHCEEEEEEECCCCCCCCCCCCHHHCCHHHHHHHHHHCCCCCCCCCCCCCCC
QGVVHIIGPQLGLTQPGMTVVCGDSHTSTHGAFGALAFGIGTSEVEHVLAT
CCEEEEECCCCCCCCCCCEEEECCCCCCCCCCCCHHHCCCCHHHHHHHHCC
>Mature Secondary Structure 
SLLVGRSVRRSVLTIATEDHNIPTVGSIEPIVDPVSRAQVEALRANAPEFGVPPLGDAD
CCCCCCHHHCEEEEEEECCCCCCCCCCCCHHHCCHHHHHHHHHHCCCCCCCCCCCCCCC
QGVVHIIGPQLGLTQPGMTVVCGDSHTSTHGAFGALAFGIGTSEVEHVLAT
CCEEEEECCCCCCCCCCCEEEECCCCCCCCCCCCHHHCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA