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The map label for this gene is leuC [H]

Identifier: 50842846

GI number: 50842846

Start: 1485769

End: 1486176

Strand: Reverse

Name: leuC [H]

Synonym: PPA1362

Alternate gene names: 50842846

Gene position: 1486176-1485769 (Counterclockwise)

Preceding gene: 50842847

Following gene: 50842845

Centisome position: 58.05

GC content: 60.29

Gene sequence:

>408_bases
ATGGCCGTCATGGTGGATGGGGAAGTGCCTGAGGAGGTCACACCTAAGGACCTCATCCTGGCCCTCATCTCCGAGATCGG
CACCGGTGGGGGACAAGGTCATATGGTCGAGTATCGCGGCGAGGCCATCGAGAAGATGTCGATGGAGGGTCGCATGACGA
TCTGCAATATGTCGATTGAGTGGGGAGTTCGCGTCGGCATGGTTGCTTCTGATGAGACCACCTTCACCTACCTCAAGGAT
CGTCCGCACGCTCCGCGTGGTGCACAGTGGGACAAGGCTGTCGCGTACTGGCGCACTCTGCGTACTGACGACGATGCGAC
CTTTGACGCTGAGATCCATGTGGACGCCTCGAATCTCGCCCCCCTTCGTTACCTGGGGTACCAACCCGGGGCAGGGAGTC
CCCCCTAG

Upstream 100 bases:

>100_bases
TACACACGGCGCCTTCGGGGCCTTAGCCTTCGGTATCGGTACCAGCGAGGTTGAGCATGTGTTGGCTACCTAGACCCTCC
CGCAGGTTCGTCCCACAACG

Downstream 100 bases:

>100_bases
GCGGTGTGGTGCCGGCCGTCGAAGACTTTGAGGACGAGGTAGCTCGCAGCGCAGCGTTTGGAGTACATGGATTTGACCCC
GACGAGATCGGTTCCCGGTT

Product: 3-isopropylmalate dehydratase large subunit fragment

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 135; Mature: 134

Protein sequence:

>135_residues
MAVMVDGEVPEEVTPKDLILALISEIGTGGGQGHMVEYRGEAIEKMSMEGRMTICNMSIEWGVRVGMVASDETTFTYLKD
RPHAPRGAQWDKAVAYWRTLRTDDDATFDAEIHVDASNLAPLRYLGYQPGAGSPP

Sequences:

>Translated_135_residues
MAVMVDGEVPEEVTPKDLILALISEIGTGGGQGHMVEYRGEAIEKMSMEGRMTICNMSIEWGVRVGMVASDETTFTYLKD
RPHAPRGAQWDKAVAYWRTLRTDDDATFDAEIHVDASNLAPLRYLGYQPGAGSPP
>Mature_134_residues
AVMVDGEVPEEVTPKDLILALISEIGTGGGQGHMVEYRGEAIEKMSMEGRMTICNMSIEWGVRVGMVASDETTFTYLKDR
PHAPRGAQWDKAVAYWRTLRTDDDATFDAEIHVDASNLAPLRYLGYQPGAGSPP

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786259, Length=130, Percent_Identity=51.5384615384615, Blast_Score=147, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6321429, Length=134, Percent_Identity=52.2388059701493, Blast_Score=149, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6322261, Length=134, Percent_Identity=32.089552238806, Blast_Score=65, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323335, Length=135, Percent_Identity=30.3703703703704, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 14818; Mature: 14687

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.9 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVMVDGEVPEEVTPKDLILALISEIGTGGGQGHMVEYRGEAIEKMSMEGRMTICNMSIE
CEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHCCCCCEEEEEEEEE
WGVRVGMVASDETTFTYLKDRPHAPRGAQWDKAVAYWRTLRTDDDATFDAEIHVDASNLA
CCEEEEEEECCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEEECCCCC
PLRYLGYQPGAGSPP
CEEEECCCCCCCCCC
>Mature Secondary Structure 
AVMVDGEVPEEVTPKDLILALISEIGTGGGQGHMVEYRGEAIEKMSMEGRMTICNMSIE
EEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHCCCCCEEEEEEEEE
WGVRVGMVASDETTFTYLKDRPHAPRGAQWDKAVAYWRTLRTDDDATFDAEIHVDASNLA
CCEEEEEEECCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEEECCCCC
PLRYLGYQPGAGSPP
CEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]