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The map label for this gene is uvrD [H]

Identifier: 50842794

GI number: 50842794

Start: 1420308

End: 1422428

Strand: Reverse

Name: uvrD [H]

Synonym: PPA1314

Alternate gene names: 50842794

Gene position: 1422428-1420308 (Counterclockwise)

Preceding gene: 50842798

Following gene: 50842792

Centisome position: 55.56

GC content: 58.23

Gene sequence:

>2121_bases
ATGTTGCTGTCGGTCACGACCCTTATGGTGATCGCTGTGAGAACCCGAACGGCAGATGCGATTCTGGCGGCACTGGATCC
TGAACAGGTTAAGGTTGCGAAGACCTTCGATGTTCCAGTGTGCGTCATAGCTGGTGCCGGGACAGGTAAAACTCGTGCTG
TCACTCATCGCATTGCCTACGGCGCAGTGACAGGCAAGCTTGATCCGCGTCGTACCCTCGCGGTCACTTTTACGACTAAG
GCAGCTGGCACGATGAGAGGTCGACTCGCCGATCTGGGGGTTGTTGGTGTGCAGGCTCGCACTATTCATTCTGCGGCGTT
GCGGCAGATCAAGTTTTTCTGGCCTCGTGCATATAACTGTGAGTTGCCACCGGTGAGTGATTCTCGTTTCTCGATGGTGG
CGGAGACGACCCATCGCATTGGTCTGGGCAATGACAAGGCGCTGCTGGGCGACTTGTCCGCCGAGATCTCGTGGGCGAAG
GTCTCAAATGTGCCGACTGATCAATACGCATCCCTGGCTAGGGCGGAAGGCCGGGTGGTGGCGGGAGTTTCGGCAACTGA
CGTAGGACGCGTCCTCGCTGGATATGAGGCTGTGAAGAGGGAACGCTGCGTCATTGATCTGGACGATATTTTGTTGTGCG
CGGTGGGATTGTTGGTTCAGCACCGTGACATCACTGAGGAGATTCGGGCTCGGTACCGACATTTCGTTGTCGACGAATAC
CAGGACGTTTCTCCGCTGCAGCATAGGTTGCTTGAACTGTGGTTTGGCGATCGAAATGATGTATGCGTCGTGGGAGATCC
GCACCAGGCCATTCACTCTTATGCAGGCGCACGAGCTGACTACCTCCTCGACTTCGTTGCCGATCATCCTGGCGCTAAAC
GCATCGATTTGGTTCGCAACTACCGCTCCACTCCCGAGATCGTTCAGTTGGCCAATGAAGTTCTTGTCAACCGTATGACT
CCAGAGGAGGCTCTGGAACATGGCAGGGGAGTCACATTGGTTTCGCGGGGTCGATCCGGTCCCGAGCCCATCTATCAAGC
TCTCGGGGACGATGCCTCCGAAGCTTCAGCTGTAGCAATGTGGATTGAATCGCGCCATGCTGCGGGAATCCCATGGTCTG
AGATTGCCGTCTTGTACCGGATTAATTCTCAGGCAGCCCAGCTTGAGACCGCGCTGGCCGAGAGGTCCATCCCATATCTT
GTGAAGGGGTCTGAGCGCTTCTATGACAGGGGCGAGGTGCGACGCAGCCTTGACGCTTTGGCTCGGCTTGCTGCTGATGA
ACCAGGTGCGGATCCGCTACGGGCTTTTGATCGGATACTCGCAGCTCAAGGTTGGTCGGCTATTGCGCCCGACGGAGAAG
GTGATGTCCGGCAACGGTGGGAATCGTTGCAAGCTTTGCACGACCTGACGGTCTCGGTTGTAGACGACGGGACGAGGACG
CTGGAAGAACTGGCAGCCGTGTTCTCCCGTCGGGCGGCCACTCAGGAGGCGCCGGTCGGCGACGGCGTTACCTTGGCAAC
CTTGCATGCATCTAAGGGGCTCGAATGGGATGTCGTGGCCCTCTATGGCATAAATGACACGATGGTGCCCTTCATCATGG
CTGAAGCTCCGGCTGAGGTGGCTTCGGAACGCCGGTTGTTGCATGTTGGCGTAACTCGAGCACGACGAGACCTGTGGGTC
TCGTACTCGTGGTCTGGAGGGAACCGGGACCGGCAGGGGATCTCGCGATTTTTGCGCGGCCTCCCTGGGACGGGGGAAAC
GACCGACAAACCCCCGCGACATCGCCGGAAACGTCGTGCCACGATCACAGTCTGCTCGGTGTGCGGCAAAGCCCTTACGG
CGGCCAGAGACCGAAAACTCGGCCATCACCTTGCCTGCGAGGTGGGGGTCGACCCAGTTCTCGTCGAGAGGTTGCGGCAG
TGGCGCAGCGAACGCGCGGAAGCCGATCGAGTGCCAGCTTTCGTCATTCTCACGGACGCTACCTTGCTGGGCGTGGCGGA
GAAACGACCTAACTCTATGGAAAGTTTATTGCAGGTCCCCGGAATTGGTAGGCGCAAGGCCGATCTTTACGCGGCTGATC
TCATCAAGGTGCTGCACGGTTCCTCGGATAACCAACAGTGA

Upstream 100 bases:

>100_bases
GGCCTTCTGCCGAACTGATTAATCAGTTTAGTGGCGAGTCATGACAAGTTGACGAGTCAGCTGTGGTACACGTGCGGAAC
ACTCACAATGCCACGGCGGC

Downstream 100 bases:

>100_bases
CATCCAGGCTGGGCTGGCACCTCAATACTGCGTTCGCCGGCAATGGTACGCACGTGGAAGGTTTTCGGTCGACGGGAGAC
CGTCGAGTCGCGGATAGATA

Product: putative DNA helicase II-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 706; Mature: 706

Protein sequence:

>706_residues
MLLSVTTLMVIAVRTRTADAILAALDPEQVKVAKTFDVPVCVIAGAGTGKTRAVTHRIAYGAVTGKLDPRRTLAVTFTTK
AAGTMRGRLADLGVVGVQARTIHSAALRQIKFFWPRAYNCELPPVSDSRFSMVAETTHRIGLGNDKALLGDLSAEISWAK
VSNVPTDQYASLARAEGRVVAGVSATDVGRVLAGYEAVKRERCVIDLDDILLCAVGLLVQHRDITEEIRARYRHFVVDEY
QDVSPLQHRLLELWFGDRNDVCVVGDPHQAIHSYAGARADYLLDFVADHPGAKRIDLVRNYRSTPEIVQLANEVLVNRMT
PEEALEHGRGVTLVSRGRSGPEPIYQALGDDASEASAVAMWIESRHAAGIPWSEIAVLYRINSQAAQLETALAERSIPYL
VKGSERFYDRGEVRRSLDALARLAADEPGADPLRAFDRILAAQGWSAIAPDGEGDVRQRWESLQALHDLTVSVVDDGTRT
LEELAAVFSRRAATQEAPVGDGVTLATLHASKGLEWDVVALYGINDTMVPFIMAEAPAEVASERRLLHVGVTRARRDLWV
SYSWSGGNRDRQGISRFLRGLPGTGETTDKPPRHRRKRRATITVCSVCGKALTAARDRKLGHHLACEVGVDPVLVERLRQ
WRSERAEADRVPAFVILTDATLLGVAEKRPNSMESLLQVPGIGRRKADLYAADLIKVLHGSSDNQQ

Sequences:

>Translated_706_residues
MLLSVTTLMVIAVRTRTADAILAALDPEQVKVAKTFDVPVCVIAGAGTGKTRAVTHRIAYGAVTGKLDPRRTLAVTFTTK
AAGTMRGRLADLGVVGVQARTIHSAALRQIKFFWPRAYNCELPPVSDSRFSMVAETTHRIGLGNDKALLGDLSAEISWAK
VSNVPTDQYASLARAEGRVVAGVSATDVGRVLAGYEAVKRERCVIDLDDILLCAVGLLVQHRDITEEIRARYRHFVVDEY
QDVSPLQHRLLELWFGDRNDVCVVGDPHQAIHSYAGARADYLLDFVADHPGAKRIDLVRNYRSTPEIVQLANEVLVNRMT
PEEALEHGRGVTLVSRGRSGPEPIYQALGDDASEASAVAMWIESRHAAGIPWSEIAVLYRINSQAAQLETALAERSIPYL
VKGSERFYDRGEVRRSLDALARLAADEPGADPLRAFDRILAAQGWSAIAPDGEGDVRQRWESLQALHDLTVSVVDDGTRT
LEELAAVFSRRAATQEAPVGDGVTLATLHASKGLEWDVVALYGINDTMVPFIMAEAPAEVASERRLLHVGVTRARRDLWV
SYSWSGGNRDRQGISRFLRGLPGTGETTDKPPRHRRKRRATITVCSVCGKALTAARDRKLGHHLACEVGVDPVLVERLRQ
WRSERAEADRVPAFVILTDATLLGVAEKRPNSMESLLQVPGIGRRKADLYAADLIKVLHGSSDNQQ
>Mature_706_residues
MLLSVTTLMVIAVRTRTADAILAALDPEQVKVAKTFDVPVCVIAGAGTGKTRAVTHRIAYGAVTGKLDPRRTLAVTFTTK
AAGTMRGRLADLGVVGVQARTIHSAALRQIKFFWPRAYNCELPPVSDSRFSMVAETTHRIGLGNDKALLGDLSAEISWAK
VSNVPTDQYASLARAEGRVVAGVSATDVGRVLAGYEAVKRERCVIDLDDILLCAVGLLVQHRDITEEIRARYRHFVVDEY
QDVSPLQHRLLELWFGDRNDVCVVGDPHQAIHSYAGARADYLLDFVADHPGAKRIDLVRNYRSTPEIVQLANEVLVNRMT
PEEALEHGRGVTLVSRGRSGPEPIYQALGDDASEASAVAMWIESRHAAGIPWSEIAVLYRINSQAAQLETALAERSIPYL
VKGSERFYDRGEVRRSLDALARLAADEPGADPLRAFDRILAAQGWSAIAPDGEGDVRQRWESLQALHDLTVSVVDDGTRT
LEELAAVFSRRAATQEAPVGDGVTLATLHASKGLEWDVVALYGINDTMVPFIMAEAPAEVASERRLLHVGVTRARRDLWV
SYSWSGGNRDRQGISRFLRGLPGTGETTDKPPRHRRKRRATITVCSVCGKALTAARDRKLGHHLACEVGVDPVLVERLRQ
WRSERAEADRVPAFVILTDATLLGVAEKRPNSMESLLQVPGIGRRKADLYAADLIKVLHGSSDNQQ

Specific function: Has both ATPase and helicase activities. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. Involved in the post-incision events of nucleotide exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=434, Percent_Identity=30.4147465437788, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI48994965, Length=412, Percent_Identity=31.3106796116505, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6322369, Length=433, Percent_Identity=25.1732101616628, Blast_Score=105, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6324477, Length=438, Percent_Identity=23.0593607305936, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR002121
- InterPro:   IPR014016
- InterPro:   IPR010997 [H]

Pfam domain/function: PF00570 HRDC; PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 77321; Mature: 77321

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: PS50967 HRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLSVTTLMVIAVRTRTADAILAALDPEQVKVAKTFDVPVCVIAGAGTGKTRAVTHRIAY
CEEEHEEHHHHHHHHCCHHHHHEECCHHHEEEEEECCCCEEEEECCCCCCCHHHHHHHHH
GAVTGKLDPRRTLAVTFTTKAAGTMRGRLADLGVVGVQARTIHSAALRQIKFFWPRAYNC
HEEECCCCCCEEEEEEEECCCCHHHHHHHHHCCEEECHHHHHHHHHHHHHHHHCCCCCCC
ELPPVSDSRFSMVAETTHRIGLGNDKALLGDLSAEISWAKVSNVPTDQYASLARAEGRVV
CCCCCCCCHHHHHHHHHHHCCCCCCCCEEHHHCCCEEEHHHCCCCCHHHHHHHHCCCCEE
AGVSATDVGRVLAGYEAVKRERCVIDLDDILLCAVGLLVQHRDITEEIRARYRHFVVDEY
ECCCHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
QDVSPLQHRLLELWFGDRNDVCVVGDPHQAIHSYAGARADYLLDFVADHPGAKRIDLVRN
CCCCHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHH
YRSTPEIVQLANEVLVNRMTPEEALEHGRGVTLVSRGRSGPEPIYQALGDDASEASAVAM
CCCCHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEECCCCCCHHHHHHHCCCCHHHHHHHH
WIESRHAAGIPWSEIAVLYRINSQAAQLETALAERSIPYLVKGSERFYDRGEVRRSLDAL
HHHCCCCCCCCHHHEEEEEEECCHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHH
ARLAADEPGADPLRAFDRILAAQGWSAIAPDGEGDVRQRWESLQALHDLTVSVVDDGTRT
HHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCEEEEECCCHHH
LEELAAVFSRRAATQEAPVGDGVTLATLHASKGLEWDVVALYGINDTMVPFIMAEAPAEV
HHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCEEEEEEEECCCCHHHHHHHCCCHHH
ASERRLLHVGVTRARRDLWVSYSWSGGNRDRQGISRFLRGLPGTGETTDKPPRHRRKRRA
HHCCEEEEEHHHHHCCEEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
TITVCSVCGKALTAARDRKLGHHLACEVGVDPVLVERLRQWRSERAEADRVPAFVILTDA
HHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECC
TLLGVAEKRPNSMESLLQVPGIGRRKADLYAADLIKVLHGSSDNQQ
HHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLLSVTTLMVIAVRTRTADAILAALDPEQVKVAKTFDVPVCVIAGAGTGKTRAVTHRIAY
CEEEHEEHHHHHHHHCCHHHHHEECCHHHEEEEEECCCCEEEEECCCCCCCHHHHHHHHH
GAVTGKLDPRRTLAVTFTTKAAGTMRGRLADLGVVGVQARTIHSAALRQIKFFWPRAYNC
HEEECCCCCCEEEEEEEECCCCHHHHHHHHHCCEEECHHHHHHHHHHHHHHHHCCCCCCC
ELPPVSDSRFSMVAETTHRIGLGNDKALLGDLSAEISWAKVSNVPTDQYASLARAEGRVV
CCCCCCCCHHHHHHHHHHHCCCCCCCCEEHHHCCCEEEHHHCCCCCHHHHHHHHCCCCEE
AGVSATDVGRVLAGYEAVKRERCVIDLDDILLCAVGLLVQHRDITEEIRARYRHFVVDEY
ECCCHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
QDVSPLQHRLLELWFGDRNDVCVVGDPHQAIHSYAGARADYLLDFVADHPGAKRIDLVRN
CCCCHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHH
YRSTPEIVQLANEVLVNRMTPEEALEHGRGVTLVSRGRSGPEPIYQALGDDASEASAVAM
CCCCHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEECCCCCCHHHHHHHCCCCHHHHHHHH
WIESRHAAGIPWSEIAVLYRINSQAAQLETALAERSIPYLVKGSERFYDRGEVRRSLDAL
HHHCCCCCCCCHHHEEEEEEECCHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHH
ARLAADEPGADPLRAFDRILAAQGWSAIAPDGEGDVRQRWESLQALHDLTVSVVDDGTRT
HHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCEEEEECCCHHH
LEELAAVFSRRAATQEAPVGDGVTLATLHASKGLEWDVVALYGINDTMVPFIMAEAPAEV
HHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCEEEEEEEECCCCHHHHHHHCCCHHH
ASERRLLHVGVTRARRDLWVSYSWSGGNRDRQGISRFLRGLPGTGETTDKPPRHRRKRRA
HHCCEEEEEHHHHHCCEEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
TITVCSVCGKALTAARDRKLGHHLACEVGVDPVLVERLRQWRSERAEADRVPAFVILTDA
HHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECC
TLLGVAEKRPNSMESLLQVPGIGRRKADLYAADLIKVLHGSSDNQQ
HHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]