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The map label for this gene is trmI [H]

Identifier: 50842681

GI number: 50842681

Start: 1307978

End: 1309987

Strand: Direct

Name: trmI [H]

Synonym: PPA1201

Alternate gene names: 50842681

Gene position: 1307978-1309987 (Clockwise)

Preceding gene: 50842679

Following gene: 50842686

Centisome position: 51.09

GC content: 59.55

Gene sequence:

>2010_bases
ATGCTGCAGAGCAGCGACGTGACGGTGGACGATTTTCAAGGACTCGAAGGTTGCAACGAAATCCTCAACATCACTGTCCC
GACGTCATGTCCAGACTCCACGACGCTTACTGGGACCATGCTCATGGGGTCGGAAACCGGTGTCACGTTGACGGTGAATC
CCTTGGCGTCGACGCCATCGATCACCACCCCTATGGTGGCGCCGGTACAGTTTCGGTTACACCGCCTGCCCGAACCTCGA
AGATCGCGCCAAGGTGGTGAAACTATTCGATCCGACGGGGATTCGGTGTACAACTGTCAGAGGAGTTCCAACTTCATCCT
GAACAGTGCACCGACGCCTTCGTCATCCATCACCCGGAGGCAAACTATTTCAATGCCAAGTGAGACGCACACCGCGACCG
CCGACCGTTTTTTCGCCGCTGTCTTGTGGGATTTCGACGGTACTCTCGTCGACACTGAGTCCCGGTGGGTCGAGGCCGAA
TTGGCAATCCTCGCCTCACACGGGATCGTCTGGGAACCTGAACAAGCCAATGAGTTCACCGGGGGACCGCTGACTGATGT
TTGTGAGGCGATGGCTGCCGACTTGGGCGGTTCAGTTACCGTCGACGACGTCCGGTGTGAGCTCATCACCATGGTGTCGC
AGTTCAATCGGGAACGTGATGTGCCTTGGCGCCCCGGCGTCTTGGACCTCCTCACCGAGATCCGCGAGGCCGGTATCCCG
ATGGGGATCGTCACCGGGTCTGCTCATGCTGTTGTCGAGCCGGTATTGGAGGCCATGGCTGAGAGGATCGGTAACCCTTT
CGACACTGTCGTAACCTTCGATGATGTCATCCCGGAGACGGCTAAACCTGCCCCGGACCCTTATCTTCTTGCCGCTGAGC
GCCTCGGTGTCAGTATTACTGATTGCCTGGCCATCGAGGACTCCCCGAAGGGAGCAACCTCGGCCACCTCGGCCGGCGCT
GCGGTGCTGACCGTCACCGGACTGGCGAAGGTCGGCCCAGGTCCACGGCGGGTTCATCGTCCCGACCTGTCAGGTTTGTC
GTTGGCTGACGTGGTCAGTCTCTGGCATCTCGCTGACGAACCAGCGGCCCCGGTAGCTAACGGCGGTGGGGGAGTCCTAC
GCGCTGGCGAAAGAATCACCCTCACCGACCCAAAGGGCCGTAAGCACTCGATCGTTCTGCAACCCGGCGGCATCTTCCAC
ACCACGAAGGGGGCGGTACGTCATGACGACCTTATCGGAGGGCCTCAGGGAGTGGTCGTCACCTCTGTCGGTGGTTTGGA
CTTTTTGGCTATGCGCCCGATCCTGAGTGAGTTCACAGTATCGATGCCGCGCGAAGCCGCCATTATTTATCCCAAAGAAG
CAGCCCAGATCACCATGTGGGCCGATATCTTCCCCGGGGCACGAGTACTAGAAGCTGGCGTTGGATCCGGGGGACTGTCG
ATCCCACTGTTGCGTGCCATCGGTACGAAGGGGTGCCTACATTCCTACGAAAGGCGTCAGGAATTCGCTGATGTCGCTCG
CAAGAATGTCGAATCCTTTTACGGAGAGATTCCATCTACGTGGGATCTCAAAGTGTCTGACCTGGTCTCTGGTATCAGCT
CAGAACCAATTGACCGGGCTATCCTTGACATGCTCGCCCCATGGGAGTGCGTCGAGACGGTGGGCAAGGTTTTGGTCCCT
GGCGGTGTGCTGTGCTGTTATGTCGCAACAACGACTCAGATGGGGCGAGTCATGGATACTCTCCGCGCTCATGGCGGATG
GACTGAGCCATCGGCTACCGAGACGACGACGCGTGACTGGCATGCTGAAGGGCTGGCGATTCGTCCGGCGCACGGAACGA
CGGGCCATACCGGATTCCTTGTCATTTCACGTCGTCTTGCGCCAGGGGTGGCGGCACCGATGCGTAAACGTCGCCCCGCT
CCGGGAGCCTACGGCCCTGATTATCACGGCCCGCGTCCGCGCAATATCACCGAGCACGACCAGTGGCGGCTGCCCAAAGA
GGAGAAGTGA

Upstream 100 bases:

>100_bases
GCATGGTGCCAAGGTTAGCCCCCGCCTTCGTCTGCGACTCACGGCCCTTCGCACCGCTGTGTCCCAGCACGTTCATGTTA
TCGATAGGGCAATGGGCACG

Downstream 100 bases:

>100_bases
CGACCGTCACTGACAATTATTATCTCACGTTGTACGAGGGCCGGCGGGTCATTCTGCCGGCCCTCCTTCTTTTGTCACGG
GTGTTCTTTGCGTCGCATTG

Product: putative methyltransferase

Products: NA

Alternate protein names: tRNA(m1A58)-methyltransferase; tRNA(m1A58)MTase [H]

Number of amino acids: Translated: 669; Mature: 669

Protein sequence:

>669_residues
MLQSSDVTVDDFQGLEGCNEILNITVPTSCPDSTTLTGTMLMGSETGVTLTVNPLASTPSITTPMVAPVQFRLHRLPEPR
RSRQGGETIRSDGDSVYNCQRSSNFILNSAPTPSSSITRRQTISMPSETHTATADRFFAAVLWDFDGTLVDTESRWVEAE
LAILASHGIVWEPEQANEFTGGPLTDVCEAMAADLGGSVTVDDVRCELITMVSQFNRERDVPWRPGVLDLLTEIREAGIP
MGIVTGSAHAVVEPVLEAMAERIGNPFDTVVTFDDVIPETAKPAPDPYLLAAERLGVSITDCLAIEDSPKGATSATSAGA
AVLTVTGLAKVGPGPRRVHRPDLSGLSLADVVSLWHLADEPAAPVANGGGGVLRAGERITLTDPKGRKHSIVLQPGGIFH
TTKGAVRHDDLIGGPQGVVVTSVGGLDFLAMRPILSEFTVSMPREAAIIYPKEAAQITMWADIFPGARVLEAGVGSGGLS
IPLLRAIGTKGCLHSYERRQEFADVARKNVESFYGEIPSTWDLKVSDLVSGISSEPIDRAILDMLAPWECVETVGKVLVP
GGVLCCYVATTTQMGRVMDTLRAHGGWTEPSATETTTRDWHAEGLAIRPAHGTTGHTGFLVISRRLAPGVAAPMRKRRPA
PGAYGPDYHGPRPRNITEHDQWRLPKEEK

Sequences:

>Translated_669_residues
MLQSSDVTVDDFQGLEGCNEILNITVPTSCPDSTTLTGTMLMGSETGVTLTVNPLASTPSITTPMVAPVQFRLHRLPEPR
RSRQGGETIRSDGDSVYNCQRSSNFILNSAPTPSSSITRRQTISMPSETHTATADRFFAAVLWDFDGTLVDTESRWVEAE
LAILASHGIVWEPEQANEFTGGPLTDVCEAMAADLGGSVTVDDVRCELITMVSQFNRERDVPWRPGVLDLLTEIREAGIP
MGIVTGSAHAVVEPVLEAMAERIGNPFDTVVTFDDVIPETAKPAPDPYLLAAERLGVSITDCLAIEDSPKGATSATSAGA
AVLTVTGLAKVGPGPRRVHRPDLSGLSLADVVSLWHLADEPAAPVANGGGGVLRAGERITLTDPKGRKHSIVLQPGGIFH
TTKGAVRHDDLIGGPQGVVVTSVGGLDFLAMRPILSEFTVSMPREAAIIYPKEAAQITMWADIFPGARVLEAGVGSGGLS
IPLLRAIGTKGCLHSYERRQEFADVARKNVESFYGEIPSTWDLKVSDLVSGISSEPIDRAILDMLAPWECVETVGKVLVP
GGVLCCYVATTTQMGRVMDTLRAHGGWTEPSATETTTRDWHAEGLAIRPAHGTTGHTGFLVISRRLAPGVAAPMRKRRPA
PGAYGPDYHGPRPRNITEHDQWRLPKEEK
>Mature_669_residues
MLQSSDVTVDDFQGLEGCNEILNITVPTSCPDSTTLTGTMLMGSETGVTLTVNPLASTPSITTPMVAPVQFRLHRLPEPR
RSRQGGETIRSDGDSVYNCQRSSNFILNSAPTPSSSITRRQTISMPSETHTATADRFFAAVLWDFDGTLVDTESRWVEAE
LAILASHGIVWEPEQANEFTGGPLTDVCEAMAADLGGSVTVDDVRCELITMVSQFNRERDVPWRPGVLDLLTEIREAGIP
MGIVTGSAHAVVEPVLEAMAERIGNPFDTVVTFDDVIPETAKPAPDPYLLAAERLGVSITDCLAIEDSPKGATSATSAGA
AVLTVTGLAKVGPGPRRVHRPDLSGLSLADVVSLWHLADEPAAPVANGGGGVLRAGERITLTDPKGRKHSIVLQPGGIFH
TTKGAVRHDDLIGGPQGVVVTSVGGLDFLAMRPILSEFTVSMPREAAIIYPKEAAQITMWADIFPGARVLEAGVGSGGLS
IPLLRAIGTKGCLHSYERRQEFADVARKNVESFYGEIPSTWDLKVSDLVSGISSEPIDRAILDMLAPWECVETVGKVLVP
GGVLCCYVATTTQMGRVMDTLRAHGGWTEPSATETTTRDWHAEGLAIRPAHGTTGHTGFLVISRRLAPGVAAPMRKRRPA
PGAYGPDYHGPRPRNITEHDQWRLPKEEK

Specific function: Catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in tRNA. Is required for cell growth at extreme temperatures [H]

COG id: COG2519

COG function: function code J; tRNA(1-methyladenosine) methyltransferase and related methyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmI family [H]

Homologues:

Organism=Homo sapiens, GI222831587, Length=243, Percent_Identity=32.0987654320988, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI123173772, Length=252, Percent_Identity=30.5555555555556, Blast_Score=91, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17554716, Length=191, Percent_Identity=28.7958115183246, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI20129151, Length=189, Percent_Identity=29.6296296296296, Blast_Score=76, Evalue=8e-14,
Organism=Drosophila melanogaster, GI116008157, Length=199, Percent_Identity=31.6582914572864, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014816 [H]

Pfam domain/function: PF08704 GCD14 [H]

EC number: =2.1.1.36 [H]

Molecular weight: Translated: 71601; Mature: 71601

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQSSDVTVDDFQGLEGCNEILNITVPTSCPDSTTLTGTMLMGSETGVTLTVNPLASTPS
CCCCCCCCHHHHHCHHHHHHHEEEEECCCCCCCCEEEEEEEECCCCCEEEEECCCCCCCC
ITTPMVAPVQFRLHRLPEPRRSRQGGETIRSDGDSVYNCQRSSNFILNSAPTPSSSITRR
CCCCCCCHHHHHHHCCCCHHHHCCCCHHHHCCCCCEEECCCCCCEEEECCCCCCCCCCHH
QTISMPSETHTATADRFFAAVLWDFDGTLVDTESRWVEAELAILASHGIVWEPEQANEFT
HHCCCCCCCCHHHHHHHHHHHEECCCCCEECCCCHHHHHHHEEEECCCCEECCCCCCCCC
GGPLTDVCEAMAADLGGSVTVDDVRCELITMVSQFNRERDVPWRPGVLDLLTEIREAGIP
CCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCC
MGIVTGSAHAVVEPVLEAMAERIGNPFDTVVTFDDVIPETAKPAPDPYLLAAERLGVSIT
EEEEECCHHHHHHHHHHHHHHHHCCCHHHEEEEHHHCCCCCCCCCCCEEEEHHHCCCEEE
DCLAIEDSPKGATSATSAGAAVLTVTGLAKVGPGPRRVHRPDLSGLSLADVVSLWHLADE
HEEEECCCCCCCCCCCCCCEEEEEEECHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
PAAPVANGGGGVLRAGERITLTDPKGRKHSIVLQPGGIFHTTKGAVRHDDLIGGPQGVVV
CCCCCCCCCCCEEECCCEEEEECCCCCCEEEEECCCCEEECCCCCCCCCCCCCCCCCEEE
TSVGGLDFLAMRPILSEFTVSMPREAAIIYPKEAAQITMWADIFPGARVLEAGVGSGGLS
EECCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEECCCCHHHHHCCCCCCCCH
IPLLRAIGTKGCLHSYERRQEFADVARKNVESFYGEIPSTWDLKVSDLVSGISSEPIDRA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHCCCCCCCHHHH
ILDMLAPWECVETVGKVLVPGGVLCCYVATTTQMGRVMDTLRAHGGWTEPSATETTTRDW
HHHHHCCHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
HAEGLAIRPAHGTTGHTGFLVISRRLAPGVAAPMRKRRPAPGAYGPDYHGPRPRNITEHD
CCCCEEEECCCCCCCCCEEEEEECHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCH
QWRLPKEEK
HCCCCCCCC
>Mature Secondary Structure
MLQSSDVTVDDFQGLEGCNEILNITVPTSCPDSTTLTGTMLMGSETGVTLTVNPLASTPS
CCCCCCCCHHHHHCHHHHHHHEEEEECCCCCCCCEEEEEEEECCCCCEEEEECCCCCCCC
ITTPMVAPVQFRLHRLPEPRRSRQGGETIRSDGDSVYNCQRSSNFILNSAPTPSSSITRR
CCCCCCCHHHHHHHCCCCHHHHCCCCHHHHCCCCCEEECCCCCCEEEECCCCCCCCCCHH
QTISMPSETHTATADRFFAAVLWDFDGTLVDTESRWVEAELAILASHGIVWEPEQANEFT
HHCCCCCCCCHHHHHHHHHHHEECCCCCEECCCCHHHHHHHEEEECCCCEECCCCCCCCC
GGPLTDVCEAMAADLGGSVTVDDVRCELITMVSQFNRERDVPWRPGVLDLLTEIREAGIP
CCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCC
MGIVTGSAHAVVEPVLEAMAERIGNPFDTVVTFDDVIPETAKPAPDPYLLAAERLGVSIT
EEEEECCHHHHHHHHHHHHHHHHCCCHHHEEEEHHHCCCCCCCCCCCEEEEHHHCCCEEE
DCLAIEDSPKGATSATSAGAAVLTVTGLAKVGPGPRRVHRPDLSGLSLADVVSLWHLADE
HEEEECCCCCCCCCCCCCCEEEEEEECHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
PAAPVANGGGGVLRAGERITLTDPKGRKHSIVLQPGGIFHTTKGAVRHDDLIGGPQGVVV
CCCCCCCCCCCEEECCCEEEEECCCCCCEEEEECCCCEEECCCCCCCCCCCCCCCCCEEE
TSVGGLDFLAMRPILSEFTVSMPREAAIIYPKEAAQITMWADIFPGARVLEAGVGSGGLS
EECCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEECCCCHHHHHCCCCCCCCH
IPLLRAIGTKGCLHSYERRQEFADVARKNVESFYGEIPSTWDLKVSDLVSGISSEPIDRA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHCCCCCCCHHHH
ILDMLAPWECVETVGKVLVPGGVLCCYVATTTQMGRVMDTLRAHGGWTEPSATETTTRDW
HHHHHCCHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
HAEGLAIRPAHGTTGHTGFLVISRRLAPGVAAPMRKRRPAPGAYGPDYHGPRPRNITEHD
CCCCEEEECCCCCCCCCEEEEEECHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCH
QWRLPKEEK
HCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA