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The map label for this gene is ruvA

Identifier: 50842639

GI number: 50842639

Start: 1259658

End: 1260263

Strand: Direct

Name: ruvA

Synonym: PPA1159

Alternate gene names: 50842639

Gene position: 1259658-1260263 (Clockwise)

Preceding gene: 50842638

Following gene: 50842640

Centisome position: 49.2

GC content: 58.75

Gene sequence:

>606_bases
GTGATTTCCCATTTTTCCGGCACGGTAAGTGCTGCCGGCCCGACTTGGGTCGTCCTTGACAATCATGGGGTCGGTATCAA
GGTGCTATGCCCTCCTGCCACAGCTGCGAGTGCTCGCATCAACCAAGATATGTCCCTGCACACTTCCCTGGTTGTGAGAG
AGGAGTCCCTGACTCTTTACGGGTTCGTCGAAGCTGATGATCGCGATGCTTTTGAGCTGGTGCAAACCGCGTCGGGAGTC
GGTCCCAAATTAGCTGCAGCGATTATGAGTGTCCTTGATGCTGCTCAGCTAGCCACGGCGATTACCGAAGAAGATGACGC
AACTCTATGCCGCGTTCCCGGTATTGGTCGCAAAGGTGCGGCGAAAATGATCCTGGAGCTCAAGGACAAGGTCGCAGCCC
TTGCCCCACGCGGAGCTTCGGCGAGCGGAGCAACCCATGTGGCCGCACCGTGGCGGGAACAGGTGGCGGAAGGGCTGGTC
GGCTTGGGCTGGTCGACTAAAGACGCGGAGAAGGCCGTCGACAAGGTGGTTGCTCTCAAGGAAGCCGATCCAGCTATGAG
CATCGGCAACCTCATGCGAGCGGCGTTGCGTAGTTTGGCGCGGTGA

Upstream 100 bases:

>100_bases
GCCACCTGTGGCGTGGCGGGGCGGCTCGTCGCATTGAAGCTGCTGTGGCTACGCAACGACGCGGTCGGCGTGTGCCCCGT
TCTTGGAAGGACCTAGCCCG

Downstream 100 bases:

>100_bases
CATGTCGGATGCCTCTGGCAGACTGATAAGAGTCGTCAGGAAGGAGAATGTCGGTGGAACACTCGCCCGTCGATCCGTCG
GCCGAACCCCCTGAAAAGGC

Product: holliday junction DNA helicase RuvA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MISHFSGTVSAAGPTWVVLDNHGVGIKVLCPPATAASARINQDMSLHTSLVVREESLTLYGFVEADDRDAFELVQTASGV
GPKLAAAIMSVLDAAQLATAITEEDDATLCRVPGIGRKGAAKMILELKDKVAALAPRGASASGATHVAAPWREQVAEGLV
GLGWSTKDAEKAVDKVVALKEADPAMSIGNLMRAALRSLAR

Sequences:

>Translated_201_residues
MISHFSGTVSAAGPTWVVLDNHGVGIKVLCPPATAASARINQDMSLHTSLVVREESLTLYGFVEADDRDAFELVQTASGV
GPKLAAAIMSVLDAAQLATAITEEDDATLCRVPGIGRKGAAKMILELKDKVAALAPRGASASGATHVAAPWREQVAEGLV
GLGWSTKDAEKAVDKVVALKEADPAMSIGNLMRAALRSLAR
>Mature_201_residues
MISHFSGTVSAAGPTWVVLDNHGVGIKVLCPPATAASARINQDMSLHTSLVVREESLTLYGFVEADDRDAFELVQTASGV
GPKLAAAIMSVLDAAQLATAITEEDDATLCRVPGIGRKGAAKMILELKDKVAALAPRGASASGATHVAAPWREQVAEGLV
GLGWSTKDAEKAVDKVVALKEADPAMSIGNLMRAALRSLAR

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG0632

COG function: function code L; Holliday junction resolvasome, DNA-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvA family

Homologues:

Organism=Escherichia coli, GI1788168, Length=210, Percent_Identity=35.7142857142857, Blast_Score=99, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVA_PROAC (Q6A8K8)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_055866.1
- ProteinModelPortal:   Q6A8K8
- SMR:   Q6A8K8
- GeneID:   2932982
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA1159
- NMPDR:   fig|267747.1.peg.1143
- HOGENOM:   HBG635309
- OMA:   LSIETYV
- ProtClustDB:   CLSK2763776
- BioCyc:   PACN267747:PPA1159-MONOMER
- HAMAP:   MF_00031
- InterPro:   IPR011114
- InterPro:   IPR013849
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000085
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00278
- TIGRFAMs:   TIGR00084

Pfam domain/function: PF07499 RuvA_C; PF01330 RuvA_N; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like; SSF46929 RuvA_C-like

EC number: =3.6.4.12

Molecular weight: Translated: 20855; Mature: 20855

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISHFSGTVSAAGPTWVVLDNHGVGIKVLCPPATAASARINQDMSLHTSLVVREESLTLY
CCCCCCCCEECCCCEEEEECCCCCEEEEEECCCCCHHHHCCCCCHHHHEEEEEECCEEEE
GFVEADDRDAFELVQTASGVGPKLAAAIMSVLDAAQLATAITEEDDATLCRVPGIGRKGA
EEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHH
AKMILELKDKVAALAPRGASASGATHVAAPWREQVAEGLVGLGWSTKDAEKAVDKVVALK
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHEECCCCCCHHHHHHHHHHHHH
EADPAMSIGNLMRAALRSLAR
CCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MISHFSGTVSAAGPTWVVLDNHGVGIKVLCPPATAASARINQDMSLHTSLVVREESLTLY
CCCCCCCCEECCCCEEEEECCCCCEEEEEECCCCCHHHHCCCCCHHHHEEEEEECCEEEE
GFVEADDRDAFELVQTASGVGPKLAAAIMSVLDAAQLATAITEEDDATLCRVPGIGRKGA
EEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHH
AKMILELKDKVAALAPRGASASGATHVAAPWREQVAEGLVGLGWSTKDAEKAVDKVVALK
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHEECCCCCCHHHHHHHHHHHHH
EADPAMSIGNLMRAALRSLAR
CCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA