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The map label for this gene is glgX [H]

Identifier: 50842594

GI number: 50842594

Start: 1213662

End: 1215782

Strand: Reverse

Name: glgX [H]

Synonym: PPA1115

Alternate gene names: 50842594

Gene position: 1215782-1213662 (Counterclockwise)

Preceding gene: 50842599

Following gene: 50842593

Centisome position: 47.49

GC content: 59.22

Gene sequence:

>2121_bases
GTGACCGACACCAACACGGCGCCATTCGACACCAGCGCCTTGGGCTCGACCCTCATTGACGGCGGCTGCCGCTTTGGGTT
GTGGGCGCCTCGCGCGGAGCGTGTCGAGTTGGCCCTCGTGGCGGATGACGGGTCACAGGTTAACCACGACATGACCAGGG
ACGAGCACGGTGTCTGGACTGTTGAGGTCTCCGACGTCCAGGCCGGTCAGCGCTATGGGTACCGAGTCCACGGGCCGTGG
GATCCTGACCGCGGCATGAGGTTCAACCCTGCCAAGCTATTGCTCGACCCTTATGCCAGGGCCATCACGGCAGGAGTCGA
TTATCACGGCCCGATTATGGACCACACGCCGGAATCCAACTACGAGCCTGACCTGACCGACGATGCGACGTCGGTCCCGC
TCGCCGTCGTCATTGACGATCCCGGCCCGCCTACGCCTATTGCGCGCCGCCACGACATCAGCGAATCGGTCATCTATGAG
ACCCATGTCAAGGGCTACACCCGCCTGCACCCCCTCGTTCCTGAACACCTGCGCGGCACCTATGCCGGGCTTGCCTATCC
GGCTGTTATCGAACACCTCAAGTCAATCGGAGTAACAGCCATCGAACTACTACCCGTCCAGCAGTTCATCTCCGAGCCAT
TCATCGTTGGGCGCGGCTTATCCAATTACTGGGGTTACAACACCCTGGGTTTCTTTGCGCCGCATGCTGCCTACTGCTCC
GTCGGCTCGATGGGAACCCAGGTGCGCGAGTTCAAGGACATGGTGACGTCTTTCCACGAGGCCGGCATCGAGGTTTTCCT
CGATGTCGTCTACAACCACACTGGTGAGGGCGGCCATGAAGGACCGACTCTGTCTTTCCGCGGCATCGATCACGAGTCTT
ATTACCGCCTCACCAACGATCACCGCAATGACTATGACGTTACCGGCTGTGGCAATTCTGTGGACACCTCTCATCCGGAG
GTCCTCGCCATGGTCCTCGACTCTTTGCGCTACTGGGTCACCGAGATGGGTGTTGACGGGTTCCGTTACGACCTTGCCAC
CACCCTCATCCGCGATAAGTCCCATGGGGTCGATCAGAACCACCTATTTAAACAAGCTCTGGTCGAAGATCCGATTCTCA
ACAAGGTCAAGCACATTGCTGAGCCCTGGGACCTCGGCCCATATGGCTATCAGGTAGGTGCCTGGGGGCCACATTGGAGC
GAGTGGAACGACCGTTTCCGCAATTATGTCCGTGACTTTTGGCGTGGGGCCGTTCGCGGAGTCGAGGAGCTGGCAACCAG
GCTCTGTGGGTCACCGGACCTGTATGGTCAGCCGACCTCCAGCGTCAACTTCATTACCGCTCACGACGGCTTCACGATGC
GTGACCTGGTCACCTACAACATGAAGCATAACCAAGGCAACGGCGAGGATAACCACGACGGGTCTAATGACAACCGGGCC
TGGAACTGTGGTTGGGAGGGCGAGACTACCGATCAGTCCATCGTCATGTTGCGGCATCGTCAGGTGCGCAACCTGGTCGC
TACTTTGCTATTAGCTACCGGTGTACCAATGATCACCGCCGGTGACGAAATGGGACGCACCCAGCAGGGCAATAACAATG
CATACTGCCAGGATTCCCCTATCTCGTGGATAGATTGGGAGGATGCCGAAGCATGGTCCGATGTGACCGACCTCGTCAAG
ATAATTACGGCACTGCGTCGCGAGCACCCCGCTCTTCGTCCATCAAGGTATCGTCATCACGATCCGGTCGGGGACGCGAA
CGATTCGTACCCGCGCCGTGCTGATCTAGCGTGGTTCTCCGGCCACGGCGGGGAAATGGTAAACAACGACTGGCATGACG
AAAGCCGACGCACCCTCGGTATGTACACCAGTGACGACAATGAGGCCTTCCTCATCTGGTTTCACGCCGGTGACCGCCCG
ATCGAGATCATCTTGCCGAGCGTTCGATGGGGCGAATCGTGGCACGTTGTGGCCTCAACAGCTCTGCCTGGGGAGATTCC
CGACGAAGCCGTACCAGCAGGACTATCGGTTACTCTGCCGAGCAGATGCGTCGTCGTCATGCAAGCCTCCCCTTTCGGCC
TGACTGCACCGTTACGCGCCAAGCGCACACTTGGCGGGTGA

Upstream 100 bases:

>100_bases
CGCGCATCATCGCCCTTACGGACGCTACGACCAACCAGATTAAACTGTGACGTCAGCCCGTTAATGTTCCGGCCATTATC
CCCGACGAAAGGCCCCTATT

Downstream 100 bases:

>100_bases
GCGCGCCTTCACCCGCCGATCTCGCCGCTGTGGGTCCCTCATCCAATGGTCAGAGGCACTTCTTCCTCCTGTGAAGAGTC
GGATCAATCCAAGATTGACG

Product: putative glycogen debranching enzyme

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 706; Mature: 705

Protein sequence:

>706_residues
MTDTNTAPFDTSALGSTLIDGGCRFGLWAPRAERVELALVADDGSQVNHDMTRDEHGVWTVEVSDVQAGQRYGYRVHGPW
DPDRGMRFNPAKLLLDPYARAITAGVDYHGPIMDHTPESNYEPDLTDDATSVPLAVVIDDPGPPTPIARRHDISESVIYE
THVKGYTRLHPLVPEHLRGTYAGLAYPAVIEHLKSIGVTAIELLPVQQFISEPFIVGRGLSNYWGYNTLGFFAPHAAYCS
VGSMGTQVREFKDMVTSFHEAGIEVFLDVVYNHTGEGGHEGPTLSFRGIDHESYYRLTNDHRNDYDVTGCGNSVDTSHPE
VLAMVLDSLRYWVTEMGVDGFRYDLATTLIRDKSHGVDQNHLFKQALVEDPILNKVKHIAEPWDLGPYGYQVGAWGPHWS
EWNDRFRNYVRDFWRGAVRGVEELATRLCGSPDLYGQPTSSVNFITAHDGFTMRDLVTYNMKHNQGNGEDNHDGSNDNRA
WNCGWEGETTDQSIVMLRHRQVRNLVATLLLATGVPMITAGDEMGRTQQGNNNAYCQDSPISWIDWEDAEAWSDVTDLVK
IITALRREHPALRPSRYRHHDPVGDANDSYPRRADLAWFSGHGGEMVNNDWHDESRRTLGMYTSDDNEAFLIWFHAGDRP
IEIILPSVRWGESWHVVASTALPGEIPDEAVPAGLSVTLPSRCVVVMQASPFGLTAPLRAKRTLGG

Sequences:

>Translated_706_residues
MTDTNTAPFDTSALGSTLIDGGCRFGLWAPRAERVELALVADDGSQVNHDMTRDEHGVWTVEVSDVQAGQRYGYRVHGPW
DPDRGMRFNPAKLLLDPYARAITAGVDYHGPIMDHTPESNYEPDLTDDATSVPLAVVIDDPGPPTPIARRHDISESVIYE
THVKGYTRLHPLVPEHLRGTYAGLAYPAVIEHLKSIGVTAIELLPVQQFISEPFIVGRGLSNYWGYNTLGFFAPHAAYCS
VGSMGTQVREFKDMVTSFHEAGIEVFLDVVYNHTGEGGHEGPTLSFRGIDHESYYRLTNDHRNDYDVTGCGNSVDTSHPE
VLAMVLDSLRYWVTEMGVDGFRYDLATTLIRDKSHGVDQNHLFKQALVEDPILNKVKHIAEPWDLGPYGYQVGAWGPHWS
EWNDRFRNYVRDFWRGAVRGVEELATRLCGSPDLYGQPTSSVNFITAHDGFTMRDLVTYNMKHNQGNGEDNHDGSNDNRA
WNCGWEGETTDQSIVMLRHRQVRNLVATLLLATGVPMITAGDEMGRTQQGNNNAYCQDSPISWIDWEDAEAWSDVTDLVK
IITALRREHPALRPSRYRHHDPVGDANDSYPRRADLAWFSGHGGEMVNNDWHDESRRTLGMYTSDDNEAFLIWFHAGDRP
IEIILPSVRWGESWHVVASTALPGEIPDEAVPAGLSVTLPSRCVVVMQASPFGLTAPLRAKRTLGG
>Mature_705_residues
TDTNTAPFDTSALGSTLIDGGCRFGLWAPRAERVELALVADDGSQVNHDMTRDEHGVWTVEVSDVQAGQRYGYRVHGPWD
PDRGMRFNPAKLLLDPYARAITAGVDYHGPIMDHTPESNYEPDLTDDATSVPLAVVIDDPGPPTPIARRHDISESVIYET
HVKGYTRLHPLVPEHLRGTYAGLAYPAVIEHLKSIGVTAIELLPVQQFISEPFIVGRGLSNYWGYNTLGFFAPHAAYCSV
GSMGTQVREFKDMVTSFHEAGIEVFLDVVYNHTGEGGHEGPTLSFRGIDHESYYRLTNDHRNDYDVTGCGNSVDTSHPEV
LAMVLDSLRYWVTEMGVDGFRYDLATTLIRDKSHGVDQNHLFKQALVEDPILNKVKHIAEPWDLGPYGYQVGAWGPHWSE
WNDRFRNYVRDFWRGAVRGVEELATRLCGSPDLYGQPTSSVNFITAHDGFTMRDLVTYNMKHNQGNGEDNHDGSNDNRAW
NCGWEGETTDQSIVMLRHRQVRNLVATLLLATGVPMITAGDEMGRTQQGNNNAYCQDSPISWIDWEDAEAWSDVTDLVKI
ITALRREHPALRPSRYRHHDPVGDANDSYPRRADLAWFSGHGGEMVNNDWHDESRRTLGMYTSDDNEAFLIWFHAGDRPI
EIILPSVRWGESWHVVASTALPGEIPDEAVPAGLSVTLPSRCVVVMQASPFGLTAPLRAKRTLGG

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=659, Percent_Identity=41.4264036418816, Blast_Score=471, Evalue=1e-134,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 78831; Mature: 78700

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTNTAPFDTSALGSTLIDGGCRFGLWAPRAERVELALVADDGSQVNHDMTRDEHGVWT
CCCCCCCCCCHHHHHHHHHCCCCEECCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEE
VEVSDVQAGQRYGYRVHGPWDPDRGMRFNPAKLLLDPYARAITAGVDYHGPIMDHTPESN
EEECCCCCCHHCCEEECCCCCCCCCCEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
YEPDLTDDATSVPLAVVIDDPGPPTPIARRHDISESVIYETHVKGYTRLHPLVPEHLRGT
CCCCCCCCCCCCCEEEEECCCCCCCCCCHHCCCCHHHEEHHHHCCHHHHCCCCHHHHCCH
YAGLAYPAVIEHLKSIGVTAIELLPVQQFISEPFIVGRGLSNYWGYNTLGFFAPHAAYCS
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEECCCHHHCCCCCHHHHCCCHHHHC
VGSMGTQVREFKDMVTSFHEAGIEVFLDVVYNHTGEGGHEGPTLSFRGIDHESYYRLTND
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCHHHEEECCC
HRNDYDVTGCGNSVDTSHPEVLAMVLDSLRYWVTEMGVDGFRYDLATTLIRDKSHGVDQN
CCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHH
HLFKQALVEDPILNKVKHIAEPWDLGPYGYQVGAWGPHWSEWNDRFRNYVRDFWRGAVRG
HHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
VEELATRLCGSPDLYGQPTSSVNFITAHDGFTMRDLVTYNMKHNQGNGEDNHDGSNDNRA
HHHHHHHHCCCCCCCCCCCCCEEEEEECCCCEEHHHEEEEEECCCCCCCCCCCCCCCCCE
WNCGWEGETTDQSIVMLRHRQVRNLVATLLLATGVPMITAGDEMGRTQQGNNNAYCQDSP
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHCCCCCCCCCCEECCCC
ISWIDWEDAEAWSDVTDLVKIITALRREHPALRPSRYRHHDPVGDANDSYPRRADLAWFS
CCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
GHGGEMVNNDWHDESRRTLGMYTSDDNEAFLIWFHAGDRPIEIILPSVRWGESWHVVAST
CCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCEEEEEECCCCCCCCEEEEEEC
ALPGEIPDEAVPAGLSVTLPSRCVVVMQASPFGLTAPLRAKRTLGG
CCCCCCCHHHCCCCEEEECCCEEEEEEECCCCCCCCCCHHHHCCCC
>Mature Secondary Structure 
TDTNTAPFDTSALGSTLIDGGCRFGLWAPRAERVELALVADDGSQVNHDMTRDEHGVWT
CCCCCCCCCHHHHHHHHHCCCCEECCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEE
VEVSDVQAGQRYGYRVHGPWDPDRGMRFNPAKLLLDPYARAITAGVDYHGPIMDHTPESN
EEECCCCCCHHCCEEECCCCCCCCCCEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
YEPDLTDDATSVPLAVVIDDPGPPTPIARRHDISESVIYETHVKGYTRLHPLVPEHLRGT
CCCCCCCCCCCCCEEEEECCCCCCCCCCHHCCCCHHHEEHHHHCCHHHHCCCCHHHHCCH
YAGLAYPAVIEHLKSIGVTAIELLPVQQFISEPFIVGRGLSNYWGYNTLGFFAPHAAYCS
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEECCCHHHCCCCCHHHHCCCHHHHC
VGSMGTQVREFKDMVTSFHEAGIEVFLDVVYNHTGEGGHEGPTLSFRGIDHESYYRLTND
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCHHHEEECCC
HRNDYDVTGCGNSVDTSHPEVLAMVLDSLRYWVTEMGVDGFRYDLATTLIRDKSHGVDQN
CCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHH
HLFKQALVEDPILNKVKHIAEPWDLGPYGYQVGAWGPHWSEWNDRFRNYVRDFWRGAVRG
HHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
VEELATRLCGSPDLYGQPTSSVNFITAHDGFTMRDLVTYNMKHNQGNGEDNHDGSNDNRA
HHHHHHHHCCCCCCCCCCCCCEEEEEECCCCEEHHHEEEEEECCCCCCCCCCCCCCCCCE
WNCGWEGETTDQSIVMLRHRQVRNLVATLLLATGVPMITAGDEMGRTQQGNNNAYCQDSP
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHCCCCCCCCCCEECCCC
ISWIDWEDAEAWSDVTDLVKIITALRREHPALRPSRYRHHDPVGDANDSYPRRADLAWFS
CCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
GHGGEMVNNDWHDESRRTLGMYTSDDNEAFLIWFHAGDRPIEIILPSVRWGESWHVVAST
CCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCEEEEEECCCCCCCCEEEEEEC
ALPGEIPDEAVPAGLSVTLPSRCVVVMQASPFGLTAPLRAKRTLGG
CCCCCCCHHHCCCCEEEECCCEEEEEEECCCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]