Click here to switch to the map view.

The map label for this gene is carA [H]

Identifier: 50842482

GI number: 50842482

Start: 1081872

End: 1082960

Strand: Direct

Name: carA [H]

Synonym: PPA0999

Alternate gene names: 50842482

Gene position: 1081872-1082960 (Clockwise)

Preceding gene: 50842481

Following gene: 50842483

Centisome position: 42.26

GC content: 60.51

Gene sequence:

>1089_bases
GTGAGCAGACGCACCCTGTTATTGGCCGACGGGACAAGCTACCAGGGCGAGGCTTTCGGGGCCGATCGTGAGGTGAGTGC
CGAGGTCGTCTTCACTACCGGCATGACCGGCTACCAGGAAACTTTGACCGACCAGTCCTATAACGGACAAATCGTCACCT
TCACCTACCCCCTTATTGGCAATTGCGGAATCAACCGTGATGATATGGAGTCGCTATTCCCGACGACCGCCGGGGTCATC
TGCCGCGAGGTGCCGCGACTGGCTAGCAACTGGCGCAATCGGCGTAGCCTTGGCGAATTTCTCACCACCCACGGCATCCC
TGGTCTGGCTGGGATTGATACTCGTGCTTTGGTGCGTCGGTTGCGCACCGCGGGAGTCATGAAGGGTGTGTTGCTGGCCC
CTGACGCTGATCTCGACGCTGAGCTGGGGAAGTTGCGAAACATCCACTTGCCTACCGACCAGATCGCCCAGGTGTCCACC
CGCACCGCCTACCCGTCACCCTTGGGCGGGCGCAGTGTGGTGCTCTTCGACTTCGGTCTTAAGCACTCCATCTTGCGGGA
GCTGAGCAGGCGAGGTTGTGCCGTGACCGTTGTGCCGTGGAACATCTCAGCCGATCGGGTGCGGGCCCTGGCGCCCGACG
GCATCATGCTTTCTAACGGCCCCGGGGACCCGAAATCCATCCCGGGGGTACAGGATGTGGTGCGAGAACTGCAGGAGGAG
TACCCGGTCTTCGGTATTTGCATGGGCCATCAGATCTTTGCTCTGGCTAACGGTGCCGATACCTATAAGCTGCGTTTCGG
CCATCGCGGCTTCAACCACCCCGTCCGCGAGCTGTCGACCGGTCGGATCGACTTCACCAGCCAGAACCACGGCTACGTCG
TTGATCCCGAATCGGTGTCCGGCACCGAGTTGGAGGTGACCCACATCGAGATTAATGATGAGACCGTCATGGGTGTGCGT
CATACCCGACTCGGTTCTTTTTCGGTGCAGTACCACCCCGACGCCGCCGCTGGACCTCACGATGCCGCTCACGTCTTCGA
CGACTTTGTCGCTCTCATGGACGCCAATCACACTGGAGGCCAGAACTGA

Upstream 100 bases:

>100_bases
TCCGCGGTGACTGCGTGCTGACGATGGTCGGTGGCCAGACGGTTTACCGTCGCGGCCAGCCGATAGGGAAGACTGACGAC
CACGAGATGGGAGACCCGCA

Downstream 100 bases:

>100_bases
TGCCCCGCCGTACCGACCTGCACCGCATTCTCGTCATCGGGTCCGGGCCGATCCTCATCGGTCAGGCTGCCGAGTTCGAC
TACGCCGGTACTCAGGCCTG

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MSRRTLLLADGTSYQGEAFGADREVSAEVVFTTGMTGYQETLTDQSYNGQIVTFTYPLIGNCGINRDDMESLFPTTAGVI
CREVPRLASNWRNRRSLGEFLTTHGIPGLAGIDTRALVRRLRTAGVMKGVLLAPDADLDAELGKLRNIHLPTDQIAQVST
RTAYPSPLGGRSVVLFDFGLKHSILRELSRRGCAVTVVPWNISADRVRALAPDGIMLSNGPGDPKSIPGVQDVVRELQEE
YPVFGICMGHQIFALANGADTYKLRFGHRGFNHPVRELSTGRIDFTSQNHGYVVDPESVSGTELEVTHIEINDETVMGVR
HTRLGSFSVQYHPDAAAGPHDAAHVFDDFVALMDANHTGGQN

Sequences:

>Translated_362_residues
MSRRTLLLADGTSYQGEAFGADREVSAEVVFTTGMTGYQETLTDQSYNGQIVTFTYPLIGNCGINRDDMESLFPTTAGVI
CREVPRLASNWRNRRSLGEFLTTHGIPGLAGIDTRALVRRLRTAGVMKGVLLAPDADLDAELGKLRNIHLPTDQIAQVST
RTAYPSPLGGRSVVLFDFGLKHSILRELSRRGCAVTVVPWNISADRVRALAPDGIMLSNGPGDPKSIPGVQDVVRELQEE
YPVFGICMGHQIFALANGADTYKLRFGHRGFNHPVRELSTGRIDFTSQNHGYVVDPESVSGTELEVTHIEINDETVMGVR
HTRLGSFSVQYHPDAAAGPHDAAHVFDDFVALMDANHTGGQN
>Mature_361_residues
SRRTLLLADGTSYQGEAFGADREVSAEVVFTTGMTGYQETLTDQSYNGQIVTFTYPLIGNCGINRDDMESLFPTTAGVIC
REVPRLASNWRNRRSLGEFLTTHGIPGLAGIDTRALVRRLRTAGVMKGVLLAPDADLDAELGKLRNIHLPTDQIAQVSTR
TAYPSPLGGRSVVLFDFGLKHSILRELSRRGCAVTVVPWNISADRVRALAPDGIMLSNGPGDPKSIPGVQDVVRELQEEY
PVFGICMGHQIFALANGADTYKLRFGHRGFNHPVRELSTGRIDFTSQNHGYVVDPESVSGTELEVTHIEINDETVMGVRH
TRLGSFSVQYHPDAAAGPHDAAHVFDDFVALMDANHTGGQN

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=373, Percent_Identity=41.0187667560322, Blast_Score=253, Evalue=2e-67,
Organism=Homo sapiens, GI169790915, Length=362, Percent_Identity=34.2541436464088, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI21361331, Length=362, Percent_Identity=34.2541436464088, Blast_Score=204, Evalue=1e-52,
Organism=Escherichia coli, GI1786215, Length=370, Percent_Identity=44.0540540540541, Blast_Score=291, Evalue=3e-80,
Organism=Caenorhabditis elegans, GI193204318, Length=371, Percent_Identity=38.544474393531, Blast_Score=240, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6324878, Length=369, Percent_Identity=38.7533875338753, Blast_Score=252, Evalue=7e-68,
Organism=Saccharomyces cerevisiae, GI6322331, Length=386, Percent_Identity=35.4922279792746, Blast_Score=229, Evalue=4e-61,
Organism=Saccharomyces cerevisiae, GI6322638, Length=176, Percent_Identity=31.8181818181818, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI45555749, Length=370, Percent_Identity=40.2702702702703, Blast_Score=238, Evalue=6e-63,
Organism=Drosophila melanogaster, GI24642586, Length=370, Percent_Identity=40.2702702702703, Blast_Score=237, Evalue=9e-63,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 39474; Mature: 39343

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRTLLLADGTSYQGEAFGADREVSAEVVFTTGMTGYQETLTDQSYNGQIVTFTYPLIG
CCCCEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHCCCCCCCEEEEEEECEEC
NCGINRDDMESLFPTTAGVICREVPRLASNWRNRRSLGEFLTTHGIPGLAGIDTRALVRR
CCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
LRTAGVMKGVLLAPDADLDAELGKLRNIHLPTDQIAQVSTRTAYPSPLGGRSVVLFDFGL
HHHHHHHCCEEECCCCCCCHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEEECCH
KHSILRELSRRGCAVTVVPWNISADRVRALAPDGIMLSNGPGDPKSIPGVQDVVRELQEE
HHHHHHHHHHCCCEEEEEECCCCHHHHHEECCCCEEECCCCCCCCCCCCHHHHHHHHHHH
YPVFGICMGHQIFALANGADTYKLRFGHRGFNHPVRELSTGRIDFTSQNHGYVVDPESVS
CCEEEEEECCEEEEEECCCCEEEEEECCCCCCCCHHHHCCCEEEEECCCCCEEECCCCCC
GTELEVTHIEINDETVMGVRHTRLGSFSVQYHPDAAAGPHDAAHVFDDFVALMDANHTGG
CCEEEEEEEEECCCEEEEEEEEECCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCC
QN
CC
>Mature Secondary Structure 
SRRTLLLADGTSYQGEAFGADREVSAEVVFTTGMTGYQETLTDQSYNGQIVTFTYPLIG
CCCEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHCCCCCCCEEEEEEECEEC
NCGINRDDMESLFPTTAGVICREVPRLASNWRNRRSLGEFLTTHGIPGLAGIDTRALVRR
CCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
LRTAGVMKGVLLAPDADLDAELGKLRNIHLPTDQIAQVSTRTAYPSPLGGRSVVLFDFGL
HHHHHHHCCEEECCCCCCCHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEEECCH
KHSILRELSRRGCAVTVVPWNISADRVRALAPDGIMLSNGPGDPKSIPGVQDVVRELQEE
HHHHHHHHHHCCCEEEEEECCCCHHHHHEECCCCEEECCCCCCCCCCCCHHHHHHHHHHH
YPVFGICMGHQIFALANGADTYKLRFGHRGFNHPVRELSTGRIDFTSQNHGYVVDPESVS
CCEEEEEECCEEEEEECCCCEEEEEECCCCCCCCHHHHCCCEEEEECCCCCEEECCCCCC
GTELEVTHIEINDETVMGVRHTRLGSFSVQYHPDAAAGPHDAAHVFDDFVALMDANHTGG
CCEEEEEEEEECCCEEEEEEEEECCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCC
QN
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11463916 [H]