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The map label for this gene is pyrB

Identifier: 50842480

GI number: 50842480

Start: 1079597

End: 1080541

Strand: Direct

Name: pyrB

Synonym: PPA0997

Alternate gene names: 50842480

Gene position: 1079597-1080541 (Clockwise)

Preceding gene: 50842479

Following gene: 50842481

Centisome position: 42.17

GC content: 56.19

Gene sequence:

>945_bases
ATGAGCACTGAGGTTGTTGAAGAGTTCGACGCCGACTACACCGGACGTCACTTTTTGTCGGTCGAAGATATGTCCAGTGA
TGACGTCATGCGGATCATCGAGCGTGGTCGGCAATTCAAGGCCGGTGACGCTCCGCGCGTGGAGCCGGGTCACGTCGCTA
TCAACATGTTCTTCGAGAACTCCACCCGAACGATGACCAGCTTTCAGATGGCTGAGCACCGTCTCGGCATGAAGATCCTC
GACTTCGATCCGGGCCACTCCAGCGTCACCAAGGGGGAGAGCCTCTACGACTCGGTGCGCACCGTCGACGCCATTGGCGC
CGAGGTTGCCGTCATACGTCACTCCACCAACCACTACTACGATTACCTGCTCGCAACCGGGATGCTCGGCCTGTCGGTCG
TCAATGGTGGGGATGGATCTGGTCAACACCCTAGTCAGTGCATGCTGGACCTCATGACTATCGCTGAGGAGTTTGGCCAT
GTCGACGGTCTAACGGTTGCGATCAGCGGCGATATCGTGCACTCGCGAGTAGCTCGTTCTGATGCTCAAATCCTTACCAG
GCTTGGGGCCAACGTTGTCTTTACTGGACCGCGTGAGTGGATGGACCATGACGTCACCCGACTTGGCACGATGGCGACTC
TCGACGAGGTCATTGCTGATGTCGACGTCGCGATGATGCTACGCGTCCAGCACGAGAGATTCGATGCTGGCCCAGACTTC
TCTGCAACTGATTACTTACATACGTTTGGGCTCACAGACGAGCGCGCAGAGCGAATGAAGCCTCATGCGATCATCATGCA
CCCGGCTCCTGTAAACCGAGGATGTGAAATTTCCGGTCACCTCGTAGAGGCTCCCTCGTCTCGTATTTTTGAGCAGATGG
GGAACGGAGTTATGGTGCGAATGGCTATTTTGGAACAGGTGTTGCACGGCAGGGAGACAAAATGA

Upstream 100 bases:

>100_bases
TTGGTAAAAACATCCCCACTTCGTTGGGTGAGGCCGTGGACGTCCACGTCACCGAAGTTGATGGCGAAGACTCCGTCACG
ATTCGAAAGGTGGCTTCCCG

Downstream 100 bases:

>100_bases
GCCGCCGGGTCCTCCTCACCGGGCTGACCATGCTGGTGGGAGCCACCCCGGTTTCCTCCCAGCTCCTCATCCGTGATGAG
CGGATCGAGGCTATTTCACG

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MSTEVVEEFDADYTGRHFLSVEDMSSDDVMRIIERGRQFKAGDAPRVEPGHVAINMFFENSTRTMTSFQMAEHRLGMKIL
DFDPGHSSVTKGESLYDSVRTVDAIGAEVAVIRHSTNHYYDYLLATGMLGLSVVNGGDGSGQHPSQCMLDLMTIAEEFGH
VDGLTVAISGDIVHSRVARSDAQILTRLGANVVFTGPREWMDHDVTRLGTMATLDEVIADVDVAMMLRVQHERFDAGPDF
SATDYLHTFGLTDERAERMKPHAIIMHPAPVNRGCEISGHLVEAPSSRIFEQMGNGVMVRMAILEQVLHGRETK

Sequences:

>Translated_314_residues
MSTEVVEEFDADYTGRHFLSVEDMSSDDVMRIIERGRQFKAGDAPRVEPGHVAINMFFENSTRTMTSFQMAEHRLGMKIL
DFDPGHSSVTKGESLYDSVRTVDAIGAEVAVIRHSTNHYYDYLLATGMLGLSVVNGGDGSGQHPSQCMLDLMTIAEEFGH
VDGLTVAISGDIVHSRVARSDAQILTRLGANVVFTGPREWMDHDVTRLGTMATLDEVIADVDVAMMLRVQHERFDAGPDF
SATDYLHTFGLTDERAERMKPHAIIMHPAPVNRGCEISGHLVEAPSSRIFEQMGNGVMVRMAILEQVLHGRETK
>Mature_313_residues
STEVVEEFDADYTGRHFLSVEDMSSDDVMRIIERGRQFKAGDAPRVEPGHVAINMFFENSTRTMTSFQMAEHRLGMKILD
FDPGHSSVTKGESLYDSVRTVDAIGAEVAVIRHSTNHYYDYLLATGMLGLSVVNGGDGSGQHPSQCMLDLMTIAEEFGHV
DGLTVAISGDIVHSRVARSDAQILTRLGANVVFTGPREWMDHDVTRLGTMATLDEVIADVDVAMMLRVQHERFDAGPDFS
ATDYLHTFGLTDERAERMKPHAIIMHPAPVNRGCEISGHLVEAPSSRIFEQMGNGVMVRMAILEQVLHGRETK

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=307, Percent_Identity=34.8534201954397, Blast_Score=162, Evalue=5e-40,
Organism=Escherichia coli, GI2367364, Length=307, Percent_Identity=32.8990228013029, Blast_Score=148, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI193204318, Length=330, Percent_Identity=31.8181818181818, Blast_Score=134, Evalue=8e-32,
Organism=Saccharomyces cerevisiae, GI6322331, Length=310, Percent_Identity=32.258064516129, Blast_Score=147, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24642586, Length=316, Percent_Identity=36.0759493670886, Blast_Score=162, Evalue=2e-40,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_PROAC (Q6A917)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_055707.1
- ProteinModelPortal:   Q6A917
- SMR:   Q6A917
- GeneID:   2930938
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA0997
- NMPDR:   fig|267747.1.peg.984
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- ProtClustDB:   PRK00856
- BioCyc:   PACN267747:PPA0997-MONOMER
- BRENDA:   2.1.3.2
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 34739; Mature: 34608

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
6.1 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.8 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTEVVEEFDADYTGRHFLSVEDMSSDDVMRIIERGRQFKAGDAPRVEPGHVAINMFFEN
CCCHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEEC
STRTMTSFQMAEHRLGMKILDFDPGHSSVTKGESLYDSVRTVDAIGAEVAVIRHSTNHYY
CCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCHHH
DYLLATGMLGLSVVNGGDGSGQHPSQCMLDLMTIAEEFGHVDGLTVAISGDIVHSRVARS
HHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHH
DAQILTRLGANVVFTGPREWMDHDVTRLGTMATLDEVIADVDVAMMLRVQHERFDAGPDF
HHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SATDYLHTFGLTDERAERMKPHAIIMHPAPVNRGCEISGHLVEAPSSRIFEQMGNGVMVR
CCHHHHHHCCCCHHHHHHCCCCEEEECCCCCCCCCCCCCEEEECCHHHHHHHHCCCEEEH
MAILEQVLHGRETK
HHHHHHHHCCCCCC
>Mature Secondary Structure 
STEVVEEFDADYTGRHFLSVEDMSSDDVMRIIERGRQFKAGDAPRVEPGHVAINMFFEN
CCHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEEC
STRTMTSFQMAEHRLGMKILDFDPGHSSVTKGESLYDSVRTVDAIGAEVAVIRHSTNHYY
CCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCHHH
DYLLATGMLGLSVVNGGDGSGQHPSQCMLDLMTIAEEFGHVDGLTVAISGDIVHSRVARS
HHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHH
DAQILTRLGANVVFTGPREWMDHDVTRLGTMATLDEVIADVDVAMMLRVQHERFDAGPDF
HHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SATDYLHTFGLTDERAERMKPHAIIMHPAPVNRGCEISGHLVEAPSSRIFEQMGNGVMVR
CCHHHHHHCCCCHHHHHHCCCCEEEECCCCCCCCCCCCCEEEECCHHHHHHHHCCCEEEH
MAILEQVLHGRETK
HHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA