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The map label for this gene is pncA [H]

Identifier: 50842477

GI number: 50842477

Start: 1077237

End: 1077818

Strand: Reverse

Name: pncA [H]

Synonym: PPA0993

Alternate gene names: 50842477

Gene position: 1077818-1077237 (Counterclockwise)

Preceding gene: 50842488

Following gene: 50842476

Centisome position: 42.1

GC content: 58.76

Gene sequence:

>582_bases
ATGACTTCGTCACACCGTGCCCTTCTCGTCGTGGACGTCCAGCCGACCTTCTGCGAGGGAGGCGCTCTGGCTGTCACGGG
TGGCAATGCCTGCGCCGAGCGGATCGCGACGTATATCCCAACCCACTACAATTGTTACGACATGGTCGTCACCACTCAGG
ATTGGCATATTGATCCCGGTAACCATTTCTCCAACAACCCCGACTTTGTGGACTCCTGGCCGCCACATGGGGTTGTTGGC
ACCGACGAAGCTGAGATTCATCCGGCGATCGCTAAGATTCCTGTCGACGGACGGGTTAAGAAAGGCCAATACGCTGCAGC
CTATTCCGGTTTCGAAGGGACTACCGAGGACGGGTCAATTCTCGAGACGGTATTGCGTGATCGCGGAATCGAGGCTGTCG
ACATCGTCGGCTTGGCTCTGTCCCACTGCGTCAAAGACACGGCTCTGGACGCCCAGCGTCTCGGGTTCGTCACGACATGC
CTGACCGACCTCAGCGAGCCGGTCAGCCCAGAGATGGGCAACGCAGCCGTGGCGGTCATGGAAGATGCCGGAGTAACGGT
CACCACTAGTTCACAGCTCTGA

Upstream 100 bases:

>100_bases
CGATACTCCATGGTGTCGTAGCGATCCTGGGCTGCCTCATAGGTGCTCACTGCCATGGCGTCAGCGTACCTACAACCAGA
CGTGGTGAAAGGATGTGTCC

Downstream 100 bases:

>100_bases
GCGTCACTGACGAACCTTCGTCATCCGTCTGGTAACCATCCGAGACCATCGCCGTCAAACTGTCCGACATCCCTGCTCAA
CTCAACGTCCCTGGAGAATT

Product: pyrazinamidase/nicotinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 193; Mature: 192

Protein sequence:

>193_residues
MTSSHRALLVVDVQPTFCEGGALAVTGGNACAERIATYIPTHYNCYDMVVTTQDWHIDPGNHFSNNPDFVDSWPPHGVVG
TDEAEIHPAIAKIPVDGRVKKGQYAAAYSGFEGTTEDGSILETVLRDRGIEAVDIVGLALSHCVKDTALDAQRLGFVTTC
LTDLSEPVSPEMGNAAVAVMEDAGVTVTTSSQL

Sequences:

>Translated_193_residues
MTSSHRALLVVDVQPTFCEGGALAVTGGNACAERIATYIPTHYNCYDMVVTTQDWHIDPGNHFSNNPDFVDSWPPHGVVG
TDEAEIHPAIAKIPVDGRVKKGQYAAAYSGFEGTTEDGSILETVLRDRGIEAVDIVGLALSHCVKDTALDAQRLGFVTTC
LTDLSEPVSPEMGNAAVAVMEDAGVTVTTSSQL
>Mature_192_residues
TSSHRALLVVDVQPTFCEGGALAVTGGNACAERIATYIPTHYNCYDMVVTTQDWHIDPGNHFSNNPDFVDSWPPHGVVGT
DEAEIHPAIAKIPVDGRVKKGQYAAAYSGFEGTTEDGSILETVLRDRGIEAVDIVGLALSHCVKDTALDAQRLGFVTTCL
TDLSEPVSPEMGNAAVAVMEDAGVTVTTSSQL

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=203, Percent_Identity=33.4975369458128, Blast_Score=92, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 20463; Mature: 20332

Theoretical pI: Translated: 4.27; Mature: 4.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSHRALLVVDVQPTFCEGGALAVTGGNACAERIATYIPTHYNCYDMVVTTQDWHIDPG
CCCCCCEEEEEECCCCEECCCEEEEECCHHHHHHHHHHCCCCCCEEEEEEECCCEEECCC
NHFSNNPDFVDSWPPHGVVGTDEAEIHPAIAKIPVDGRVKKGQYAAAYSGFEGTTEDGSI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCHHH
LETVLRDRGIEAVDIVGLALSHCVKDTALDAQRLGFVTTCLTDLSEPVSPEMGNAAVAVM
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEE
EDAGVTVTTSSQL
ECCCEEEEECCCC
>Mature Secondary Structure 
TSSHRALLVVDVQPTFCEGGALAVTGGNACAERIATYIPTHYNCYDMVVTTQDWHIDPG
CCCCCEEEEEECCCCEECCCEEEEECCHHHHHHHHHHCCCCCCEEEEEEECCCEEECCC
NHFSNNPDFVDSWPPHGVVGTDEAEIHPAIAKIPVDGRVKKGQYAAAYSGFEGTTEDGSI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCHHH
LETVLRDRGIEAVDIVGLALSHCVKDTALDAQRLGFVTTCLTDLSEPVSPEMGNAAVAVM
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEE
EDAGVTVTTSSQL
ECCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]