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The map label for this gene is puuP [H]

Identifier: 50842453

GI number: 50842453

Start: 1053515

End: 1054876

Strand: Direct

Name: puuP [H]

Synonym: PPA0969

Alternate gene names: 50842453

Gene position: 1053515-1054876 (Clockwise)

Preceding gene: 50842452

Following gene: 50842455

Centisome position: 41.15

GC content: 58.88

Gene sequence:

>1362_bases
GTGAGCACAACCCTCGCCCCTGACTCCCAGTTGAAGGACCTGGGCTACCAGCCCAGCCTCAACCGTCAGCTACCCTTTGG
CTCGTTATTGGTCTATGGGCTGCTCTTCTTCGTCCCGATGGCCCCGGTGGCCGTCTTTGGGCTGGTGGTTAACCGTTCCG
GAGGCGTGCCGGTGTTGGTATATCTGGTAGCAGCAGCCGTTATGGGGCTATCAGCTATCAGCTACCGCGAAATGGCATTG
CGCTTTCCGGTAGCGGGATCGGTTTACGGGTACACCCGTTTCTCTTTGGGACGTACCCCAGGATTCATAGCCGGATGGCT
CATCCTGCTCGACTACATCCTTATGCCCGCACTGCTCACGGTGCTCTCGGCGGTCGCACTGGCCCACATTTGGCCCAGCG
TCCCCATGGGGATTTTCTCCCTTGTCTTTGTGTTCGCTTCTATGGCACTCAATTTGCAGGGCATGACTGTCACGACACGG
GTGGGCATCGTGCTGTTGACCATCCAGCTCGCAACGATCGCCTTCTTCCTGGTGTGCGTCGGACTTGGGTTGGCTGGCGG
AGGCGTTGTCTGGAGCTGGCACGCGTTGTGGCGTTCGGGAACATCATGGACTTCGGTAGCTTCCGCCGTCTCTATTGCGG
CGCTGAGTTACCTGGGGTTTGACGCCGTTGCCACCCTGAACGAGGAAGCCCGTGGTGGGGGCCGTGCCGTCGGCATAGCC
ACCCTGTCCCTTGTTGGTCTGCTCGCCGTCTTGTTCGGGATTCAGGTGATGGCTGCCGGATTGGTTTCCGATGCCACTCA
TTTCGCCCCCGGTGGGGCGACTGACCGGGCCTTCTATCACGCCGTTGACACCGTCTGCCCAGCATGGTTTATTCCGGTCT
TCACTGTCGTCAACGCCTTTGTCGCTATCTTTGCCTGTCTTGTGGTCGCGCATTCCTCGACGGCCCGGCTGATCTTCGCA
ATGGCTCGTGACAAGGTCATGCCTCCGGCGCTCTCGCGTACCAACTCCAAAGGCGTCCCGTGGGTGGCCATCATCGTCGT
AGCGACCGTTACCGCGATCCTGGCTATCACCTTTGACTCTCACGTTGAAACTATGACGACTCTGGTGACGTTCGGGGCCC
TGTCTAGTTATGTCTTGTTGCATGCTGACGTCATCGTCCAGTGCATCGTCAAGGAAAGATCACACAACTGGGTGCGTCAC
CTCATTGTCCCGATTCTTGGTGCGCTTACTCTCCTGGTTGCTCTGGCAAAGACTGAGGAGATGACCCGCATTGTGGGTTT
GGGTTGGCTGGCGGCGGGAATCATCGGTGCAGTTATTGCCCGGGTGCGTCATTCGCATCACGTGGGCACCGGGGCATCTT
GA

Upstream 100 bases:

>100_bases
AGGATGCCGCCGGAGCTCCGTTGGTCGGAGTCATTCCCGCTGGCATAGGTAAAGACCCCGAAGCCGTCCAGCAGACGGCC
CGGAACCTTGGAGAGACTCA

Downstream 100 bases:

>100_bases
CGATACCTCAGATTAACCGCAGTCTCAGTCCGAAACCCGCTCATTGCTCATATCGTGCATACCGAGATGAGGATGTTTGC
GTGGACCGTGAGTACGCCGA

Product: putative amino acid permease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MSTTLAPDSQLKDLGYQPSLNRQLPFGSLLVYGLLFFVPMAPVAVFGLVVNRSGGVPVLVYLVAAAVMGLSAISYREMAL
RFPVAGSVYGYTRFSLGRTPGFIAGWLILLDYILMPALLTVLSAVALAHIWPSVPMGIFSLVFVFASMALNLQGMTVTTR
VGIVLLTIQLATIAFFLVCVGLGLAGGGVVWSWHALWRSGTSWTSVASAVSIAALSYLGFDAVATLNEEARGGGRAVGIA
TLSLVGLLAVLFGIQVMAAGLVSDATHFAPGGATDRAFYHAVDTVCPAWFIPVFTVVNAFVAIFACLVVAHSSTARLIFA
MARDKVMPPALSRTNSKGVPWVAIIVVATVTAILAITFDSHVETMTTLVTFGALSSYVLLHADVIVQCIVKERSHNWVRH
LIVPILGALTLLVALAKTEEMTRIVGLGWLAAGIIGAVIARVRHSHHVGTGAS

Sequences:

>Translated_453_residues
MSTTLAPDSQLKDLGYQPSLNRQLPFGSLLVYGLLFFVPMAPVAVFGLVVNRSGGVPVLVYLVAAAVMGLSAISYREMAL
RFPVAGSVYGYTRFSLGRTPGFIAGWLILLDYILMPALLTVLSAVALAHIWPSVPMGIFSLVFVFASMALNLQGMTVTTR
VGIVLLTIQLATIAFFLVCVGLGLAGGGVVWSWHALWRSGTSWTSVASAVSIAALSYLGFDAVATLNEEARGGGRAVGIA
TLSLVGLLAVLFGIQVMAAGLVSDATHFAPGGATDRAFYHAVDTVCPAWFIPVFTVVNAFVAIFACLVVAHSSTARLIFA
MARDKVMPPALSRTNSKGVPWVAIIVVATVTAILAITFDSHVETMTTLVTFGALSSYVLLHADVIVQCIVKERSHNWVRH
LIVPILGALTLLVALAKTEEMTRIVGLGWLAAGIIGAVIARVRHSHHVGTGAS
>Mature_452_residues
STTLAPDSQLKDLGYQPSLNRQLPFGSLLVYGLLFFVPMAPVAVFGLVVNRSGGVPVLVYLVAAAVMGLSAISYREMALR
FPVAGSVYGYTRFSLGRTPGFIAGWLILLDYILMPALLTVLSAVALAHIWPSVPMGIFSLVFVFASMALNLQGMTVTTRV
GIVLLTIQLATIAFFLVCVGLGLAGGGVVWSWHALWRSGTSWTSVASAVSIAALSYLGFDAVATLNEEARGGGRAVGIAT
LSLVGLLAVLFGIQVMAAGLVSDATHFAPGGATDRAFYHAVDTVCPAWFIPVFTVVNAFVAIFACLVVAHSSTARLIFAM
ARDKVMPPALSRTNSKGVPWVAIIVVATVTAILAITFDSHVETMTTLVTFGALSSYVLLHADVIVQCIVKERSHNWVRHL
IVPILGALTLLVALAKTEEMTRIVGLGWLAAGIIGAVIARVRHSHHVGTGAS

Specific function: Imports putrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily [H]

Homologues:

Organism=Escherichia coli, GI87081869, Length=442, Percent_Identity=28.9592760180996, Blast_Score=148, Evalue=8e-37,
Organism=Escherichia coli, GI87082023, Length=455, Percent_Identity=27.2527472527473, Blast_Score=135, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17532491, Length=371, Percent_Identity=26.4150943396226, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17533459, Length=378, Percent_Identity=26.1904761904762, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24667468, Length=381, Percent_Identity=24.1469816272966, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 47802; Mature: 47671

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
STTLAPDSQLKDLGYQPSLNRQLPFGSLLVYGLLFFVPMAPVAVFGLVVNRSGGVPVLV
CCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH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HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]