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The map label for this gene is dnaG

Identifier: 50842441

GI number: 50842441

Start: 1039796

End: 1041667

Strand: Direct

Name: dnaG

Synonym: PPA0957

Alternate gene names: 50842441

Gene position: 1039796-1041667 (Clockwise)

Preceding gene: 50842439

Following gene: 50842442

Centisome position: 40.61

GC content: 59.46

Gene sequence:

>1872_bases
ATGGGTAGGATCGTAGGTATGGCTGGCCGGATCAACGAGGAGGACATCGCCCTGGTTCGGGAACGGGCCCGGATCGACGA
AGTCGTTGGTGAGTACGTCACCTTGCGCCATGCCGGCGGCGACTCTCTGAAAGGCCTATGTCCCTTTCACGACGAGAAGA
CCCCCAGCTTCCAAGTGACACCCTCGCGCGGCCTGTTCTACTGTTTCGGCTGCGGTGAGGGTGGCGACACGATCTCCTTC
GTTCAGAAGATCGACAACCTCACCTTCGTCGAGGCTGTTCAGCGTCTTGCCGACAAAGTTGGCGTCCAGCTACGCATCAT
CGACGATGGTCGTCCTGGCCTTGAGCCAGGCCTGCGGATGCAGATCCTCGCCGCCAATGAGGCAGCAGCGGAGTTCTTCC
AGTCCCAGATGTCGACCGATGAGGGTCGTATCGCTCGGGAATTTGTCGCTGGACGAGGTTTTTCTCCCGATGCTGCCGTG
CAGTTCGGGATGGGTTACGCACCGCGCGATGGCCACGTGCTGCGCGACCACCTGATGGCGAAGGGCTTTGCTCCCAAGGT
CCTCATTGAGTCAGGTCTGGTGCGCTCCCAAGGATGGGACTTTTTCCAGGGACGAGTGTTGTGGCCGATCAAAGACTCGG
GCAAGTCCGTGCTCGGCTTCGGTGCTAGACGGGTCTACGACGATGACCGTCTGCCCGCCAAGTACGTCAATACCCCCGAA
ACTCCGGTCTACAAAAAATCACATGTCCTGTACGGACTGGACCTGGCCCGGGCCAACATCGGCAAGAAGAGCCAGGCCGT
CATCGTCGAGGGGTACACCGACGTCATGGCGGCGCACCTGTCCGGCATAGATACCGCCGTCGCGGCCTGTGGCACTGCTT
TTGGCGATGACCATGCTCGGCTCATTCAGCGCCTCATGGGCAACAGCGGATCTCTCAACGGAGAGGTCATCTTCACTTTT
GACGGTGACGCTGCCGGCCAGAAGGCAGCCCTCAAGGTGTTTCGTGGCGATAACCAGTTCTCCTCTCAGACCTACGTCGC
CGTCGAGCCCACTGGGCTAGATCCGTGCGACCTACTCATGCAACGCGGACCAGAAGCCGTTCGAGAGCTCGTAGCACGTC
GAATCCCGCTGTACCGCTATGTCATGGAGAACACAGTTAGCCAGTTCGACCTTGATCGTGCGGATGGCCGTGTTGCTGCC
ATTAGGGCAGCAGCCCCTCTAGTTACCAGCATTCGCGACCAGTCCTTAGTGGACGGCTATCTGCGTGAGCTCTCCCAAGT
GGTAGGTACTGACGTTGACCTGGTACGTCGAGAGGTCTCGCATACCCGAAGGCAGCATCGTTCTGAGCCGCAGTTGACCA
TCGTTCCCCCTCCTGAACCGCAGCAAGGTTCGGGGATCCCGTGGCCAGATCCGGCTGATGTTTCCTTGTCAGCTGAACGA
GGATTCCTCAAACTTGCTCTGCAACATCCCGACCTCTTTAACGAAGAATGGGACCAGGTCGAGGTCGAGGATTTGCGCCA
TCCGGCCTATCAGGCCGTGTTCGAGGCTGTGCTGGCTGTGCCGCGTGGCCAGGACAGATGGACGGAGAAAGTTAGTGAGG
CGACCCCGGATCCGCAAGTGAAGCAACTTGAAGTGGCCTTGTTGGTGGAGCCGGTGCTGCGCGAAGAGCCTGACCGGGAT
TACGCAGAGGCGTACGCGGCTCGGGTGCGGCTATTGTCAATTACCGACGACATCGCCAACCTGAAATCGCGCCTGCAACG
CACGAACCCGAACCAGGACCGCAAGCTCTACAACGCCATGTTCTCTGATCTGGTAACCCTTGAGGCGCTACGCAAGAGTC
TTATTGTGGCGGGCGGAGCTGCCTCAGCCTAA

Upstream 100 bases:

>100_bases
CGGCCAGTGCGACGAGCAGCACCATTGCTAACCCGAGCTGCCAAGTAGGGTTTACGGTCACGGCGGACAGTTTTGCACCA
TTCGGGTGCTCCGGCCACAC

Downstream 100 bases:

>100_bases
CGTGGCATCTGTGGACAACTTTTTCTGTGTCCACAGATTGTCTATGAGAGGTTCCATCATGGTCCAATACGCGTCATTGT
CGGACCATGAGATACACGGA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 623; Mature: 622

Protein sequence:

>623_residues
MGRIVGMAGRINEEDIALVRERARIDEVVGEYVTLRHAGGDSLKGLCPFHDEKTPSFQVTPSRGLFYCFGCGEGGDTISF
VQKIDNLTFVEAVQRLADKVGVQLRIIDDGRPGLEPGLRMQILAANEAAAEFFQSQMSTDEGRIAREFVAGRGFSPDAAV
QFGMGYAPRDGHVLRDHLMAKGFAPKVLIESGLVRSQGWDFFQGRVLWPIKDSGKSVLGFGARRVYDDDRLPAKYVNTPE
TPVYKKSHVLYGLDLARANIGKKSQAVIVEGYTDVMAAHLSGIDTAVAACGTAFGDDHARLIQRLMGNSGSLNGEVIFTF
DGDAAGQKAALKVFRGDNQFSSQTYVAVEPTGLDPCDLLMQRGPEAVRELVARRIPLYRYVMENTVSQFDLDRADGRVAA
IRAAAPLVTSIRDQSLVDGYLRELSQVVGTDVDLVRREVSHTRRQHRSEPQLTIVPPPEPQQGSGIPWPDPADVSLSAER
GFLKLALQHPDLFNEEWDQVEVEDLRHPAYQAVFEAVLAVPRGQDRWTEKVSEATPDPQVKQLEVALLVEPVLREEPDRD
YAEAYAARVRLLSITDDIANLKSRLQRTNPNQDRKLYNAMFSDLVTLEALRKSLIVAGGAASA

Sequences:

>Translated_623_residues
MGRIVGMAGRINEEDIALVRERARIDEVVGEYVTLRHAGGDSLKGLCPFHDEKTPSFQVTPSRGLFYCFGCGEGGDTISF
VQKIDNLTFVEAVQRLADKVGVQLRIIDDGRPGLEPGLRMQILAANEAAAEFFQSQMSTDEGRIAREFVAGRGFSPDAAV
QFGMGYAPRDGHVLRDHLMAKGFAPKVLIESGLVRSQGWDFFQGRVLWPIKDSGKSVLGFGARRVYDDDRLPAKYVNTPE
TPVYKKSHVLYGLDLARANIGKKSQAVIVEGYTDVMAAHLSGIDTAVAACGTAFGDDHARLIQRLMGNSGSLNGEVIFTF
DGDAAGQKAALKVFRGDNQFSSQTYVAVEPTGLDPCDLLMQRGPEAVRELVARRIPLYRYVMENTVSQFDLDRADGRVAA
IRAAAPLVTSIRDQSLVDGYLRELSQVVGTDVDLVRREVSHTRRQHRSEPQLTIVPPPEPQQGSGIPWPDPADVSLSAER
GFLKLALQHPDLFNEEWDQVEVEDLRHPAYQAVFEAVLAVPRGQDRWTEKVSEATPDPQVKQLEVALLVEPVLREEPDRD
YAEAYAARVRLLSITDDIANLKSRLQRTNPNQDRKLYNAMFSDLVTLEALRKSLIVAGGAASA
>Mature_622_residues
GRIVGMAGRINEEDIALVRERARIDEVVGEYVTLRHAGGDSLKGLCPFHDEKTPSFQVTPSRGLFYCFGCGEGGDTISFV
QKIDNLTFVEAVQRLADKVGVQLRIIDDGRPGLEPGLRMQILAANEAAAEFFQSQMSTDEGRIAREFVAGRGFSPDAAVQ
FGMGYAPRDGHVLRDHLMAKGFAPKVLIESGLVRSQGWDFFQGRVLWPIKDSGKSVLGFGARRVYDDDRLPAKYVNTPET
PVYKKSHVLYGLDLARANIGKKSQAVIVEGYTDVMAAHLSGIDTAVAACGTAFGDDHARLIQRLMGNSGSLNGEVIFTFD
GDAAGQKAALKVFRGDNQFSSQTYVAVEPTGLDPCDLLMQRGPEAVRELVARRIPLYRYVMENTVSQFDLDRADGRVAAI
RAAAPLVTSIRDQSLVDGYLRELSQVVGTDVDLVRREVSHTRRQHRSEPQLTIVPPPEPQQGSGIPWPDPADVSLSAERG
FLKLALQHPDLFNEEWDQVEVEDLRHPAYQAVFEAVLAVPRGQDRWTEKVSEATPDPQVKQLEVALLVEPVLREEPDRDY
AEAYAARVRLLSITDDIANLKSRLQRTNPNQDRKLYNAMFSDLVTLEALRKSLIVAGGAASA

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=494, Percent_Identity=29.3522267206478, Blast_Score=203, Evalue=3e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 68643; Mature: 68511

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRIVGMAGRINEEDIALVRERARIDEVVGEYVTLRHAGGDSLKGLCPFHDEKTPSFQVT
CCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCEEEC
PSRGLFYCFGCGEGGDTISFVQKIDNLTFVEAVQRLADKVGVQLRIIDDGRPGLEPGLRM
CCCCEEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCEE
QILAANEAAAEFFQSQMSTDEGRIAREFVAGRGFSPDAAVQFGMGYAPRDGHVLRDHLMA
EEEECCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCHHHHHHHHH
KGFAPKVLIESGLVRSQGWDFFQGRVLWPIKDSGKSVLGFGARRVYDDDRLPAKYVNTPE
CCCCCHHHHHCCCCCCCCCCEECCEEEEEECCCCCCEECCCCHHCCCCCCCCHHHCCCCC
TPVYKKSHVLYGLDLARANIGKKSQAVIVEGYTDVMAAHLSGIDTAVAACGTAFGDDHAR
CCCEECCCEEEEEHHHHHCCCCCCCEEEEECHHHHHHHHHCCHHHHHHHHHCCCCCHHHH
LIQRLMGNSGSLNGEVIFTFDGDAAGQKAALKVFRGDNQFSSQTYVAVEPTGLDPCDLLM
HHHHHHCCCCCCCCEEEEEECCCCCCCHHHHEEEECCCCCCCCEEEEEECCCCCHHHHHH
QRGPEAVRELVARRIPLYRYVMENTVSQFDLDRADGRVAAIRAAAPLVTSIRDQSLVDGY
HCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHH
LRELSQVVGTDVDLVRREVSHTRRQHRSEPQLTIVPPPEPQQGSGIPWPDPADVSLSAER
HHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCEEECCC
GFLKLALQHPDLFNEEWDQVEVEDLRHPAYQAVFEAVLAVPRGQDRWTEKVSEATPDPQV
CEEEEEECCCCCCCCCCCCEEHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCH
KQLEVALLVEPVLREEPDRDYAEAYAARVRLLSITDDIANLKSRLQRTNPNQDRKLYNAM
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHH
FSDLVTLEALRKSLIVAGGAASA
HHHHHHHHHHHHHHEECCCCCCC
>Mature Secondary Structure 
GRIVGMAGRINEEDIALVRERARIDEVVGEYVTLRHAGGDSLKGLCPFHDEKTPSFQVT
CCEECCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCEEEC
PSRGLFYCFGCGEGGDTISFVQKIDNLTFVEAVQRLADKVGVQLRIIDDGRPGLEPGLRM
CCCCEEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCEE
QILAANEAAAEFFQSQMSTDEGRIAREFVAGRGFSPDAAVQFGMGYAPRDGHVLRDHLMA
EEEECCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCHHHHHHHHH
KGFAPKVLIESGLVRSQGWDFFQGRVLWPIKDSGKSVLGFGARRVYDDDRLPAKYVNTPE
CCCCCHHHHHCCCCCCCCCCEECCEEEEEECCCCCCEECCCCHHCCCCCCCCHHHCCCCC
TPVYKKSHVLYGLDLARANIGKKSQAVIVEGYTDVMAAHLSGIDTAVAACGTAFGDDHAR
CCCEECCCEEEEEHHHHHCCCCCCCEEEEECHHHHHHHHHCCHHHHHHHHHCCCCCHHHH
LIQRLMGNSGSLNGEVIFTFDGDAAGQKAALKVFRGDNQFSSQTYVAVEPTGLDPCDLLM
HHHHHHCCCCCCCCEEEEEECCCCCCCHHHHEEEECCCCCCCCEEEEEECCCCCHHHHHH
QRGPEAVRELVARRIPLYRYVMENTVSQFDLDRADGRVAAIRAAAPLVTSIRDQSLVDGY
HCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHH
LRELSQVVGTDVDLVRREVSHTRRQHRSEPQLTIVPPPEPQQGSGIPWPDPADVSLSAER
HHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCEEECCC
GFLKLALQHPDLFNEEWDQVEVEDLRHPAYQAVFEAVLAVPRGQDRWTEKVSEATPDPQV
CEEEEEECCCCCCCCCCCCEEHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCH
KQLEVALLVEPVLREEPDRDYAEAYAARVRLLSITDDIANLKSRLQRTNPNQDRKLYNAM
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHH
FSDLVTLEALRKSLIVAGGAASA
HHHHHHHHHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9422594 [H]