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The map label for this gene is proB [H]

Identifier: 50842320

GI number: 50842320

Start: 915250

End: 916485

Strand: Direct

Name: proB [H]

Synonym: PPA0834

Alternate gene names: 50842320

Gene position: 915250-916485 (Clockwise)

Preceding gene: 50842319

Following gene: 50842321

Centisome position: 35.75

GC content: 62.86

Gene sequence:

>1236_bases
GTGACCTGCGCGGAGAGTCGTGGCGCGCCCGTGTTGCCGAAGAGGAGTGGTTCGAGGGCCGTGACTGATCAGCGCGGAGC
CATATGCGGGGCCGCCACCCTGGTGGTCAAGGTCGGATCTTCCTCTTTGACTCTTCCTGGTGGCGGGATTGACGTTCGTC
GTGTTGACGACCTGGTTGATGCGCTGAGTGAGGTCATCGCGGTCGGCCGGCGCGTCGTCCTGGTGAGTTCCGGGGCGATT
GCCACCGGATTTCCCGCCATGGGGATAACTCATCGTCCGCGCACTTTGGCTGGCAAGCAAGCGGCCGCGTCGGTGGGACA
AGGTATTTTACTGGCTCACTACGCGTCGAGGTTTGCCAGTCACGGGTTGCGGGTGGGGCAGGTACTACTCACCGTCAATG
ACCTGGTGCGGCCCACCTCGTACCGCAATGCCTGGTCAACCCTCGACACTTTGCTGGGGTTGGGCGTCGTGCCGATCGTC
AACGAGAACGACACGGTCGCCACCGGAGAAATTCGGTTTGGCGATAATGATCGGCTTGCTGCCCTGGTAGCCGAGCTGGT
GCGCGCTCAAGCCCTCATCCTGCTCTCTGACGTTGACGCCTTGTACACCGCCCATCCGGATTCACCGGATGCTCGTCGCG
TGGAGGTTGTGGAGGACATCGACACATTGGATGTCGATACCCATAAAGCTGGTTCGGGGGTGGGAACCGGCGGCATGACC
ACGAAACTTGAAGCCGCCCGAATGGCCACCTGTGCCGGGGTACCGGTGGTACTCGCAGCGGCGGTGGATGCCCCGGACGT
TCTGGCTGGTGTCCCCGTGGGTACCTACTTCCGCCCGCTGGCGACGCGACGGCCCCGACGGTTGCTGTGGTTGGCCGACG
CTGCCACCCCGCAGGGACAGATCGTCATCGACGACGGAGCTGTCGAAGCTTTGACACAGCGTCATTCCTCGTTGTTGGCG
GTGGGTGTGACTCGGGTACACGGGGATTTCCAAGCAGGCGACCCAGTGACGATCCTGGCCTCCGACGGTCGAGTTGTTGG
TCGCGGTATCGCCCAGTTCTCCCATGATGAGGTGCGCGTCATGAGGGGACGTTCGAGTGCCTGGCTGGCCGCAGAAATGG
GTCCGGCGGCCTCTCGCGAGATCATCCACCGCGATGCCATGGTGCTGAGTCGTCGTCGTAAAGCTGAGCCGTCGTCGAGG
AACCAGAAAAGTAGCGGGTCAAGGGTGACGTCCTGA

Upstream 100 bases:

>100_bases
GAGGACCAGCGCTTGAAGTCCGAGCGTCCTTCGGCTACTCGTCGTCGGGAGATGGACCGCGAATATCATCGTGCTCGAGA
GGAGTATGGTCAGGTCGGTC

Downstream 100 bases:

>100_bases
CTCACGGTCTGGTTATCAGGTGAGTGATGCTCAGCTGACGAATACCATATGGCCATGAGCTCGCGAGTCATCGAACAGGC
CATAGCTGCCCGATCTGCCG

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MTCAESRGAPVLPKRSGSRAVTDQRGAICGAATLVVKVGSSSLTLPGGGIDVRRVDDLVDALSEVIAVGRRVVLVSSGAI
ATGFPAMGITHRPRTLAGKQAAASVGQGILLAHYASRFASHGLRVGQVLLTVNDLVRPTSYRNAWSTLDTLLGLGVVPIV
NENDTVATGEIRFGDNDRLAALVAELVRAQALILLSDVDALYTAHPDSPDARRVEVVEDIDTLDVDTHKAGSGVGTGGMT
TKLEAARMATCAGVPVVLAAAVDAPDVLAGVPVGTYFRPLATRRPRRLLWLADAATPQGQIVIDDGAVEALTQRHSSLLA
VGVTRVHGDFQAGDPVTILASDGRVVGRGIAQFSHDEVRVMRGRSSAWLAAEMGPAASREIIHRDAMVLSRRRKAEPSSR
NQKSSGSRVTS

Sequences:

>Translated_411_residues
MTCAESRGAPVLPKRSGSRAVTDQRGAICGAATLVVKVGSSSLTLPGGGIDVRRVDDLVDALSEVIAVGRRVVLVSSGAI
ATGFPAMGITHRPRTLAGKQAAASVGQGILLAHYASRFASHGLRVGQVLLTVNDLVRPTSYRNAWSTLDTLLGLGVVPIV
NENDTVATGEIRFGDNDRLAALVAELVRAQALILLSDVDALYTAHPDSPDARRVEVVEDIDTLDVDTHKAGSGVGTGGMT
TKLEAARMATCAGVPVVLAAAVDAPDVLAGVPVGTYFRPLATRRPRRLLWLADAATPQGQIVIDDGAVEALTQRHSSLLA
VGVTRVHGDFQAGDPVTILASDGRVVGRGIAQFSHDEVRVMRGRSSAWLAAEMGPAASREIIHRDAMVLSRRRKAEPSSR
NQKSSGSRVTS
>Mature_410_residues
TCAESRGAPVLPKRSGSRAVTDQRGAICGAATLVVKVGSSSLTLPGGGIDVRRVDDLVDALSEVIAVGRRVVLVSSGAIA
TGFPAMGITHRPRTLAGKQAAASVGQGILLAHYASRFASHGLRVGQVLLTVNDLVRPTSYRNAWSTLDTLLGLGVVPIVN
ENDTVATGEIRFGDNDRLAALVAELVRAQALILLSDVDALYTAHPDSPDARRVEVVEDIDTLDVDTHKAGSGVGTGGMTT
KLEAARMATCAGVPVVLAAAVDAPDVLAGVPVGTYFRPLATRRPRRLLWLADAATPQGQIVIDDGAVEALTQRHSSLLAV
GVTRVHGDFQAGDPVTILASDGRVVGRGIAQFSHDEVRVMRGRSSAWLAAEMGPAASREIIHRDAMVLSRRRKAEPSSRN
QKSSGSRVTS

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=290, Percent_Identity=31.7241379310345, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI21361368, Length=292, Percent_Identity=31.1643835616438, Blast_Score=103, Evalue=3e-22,
Organism=Escherichia coli, GI1786437, Length=367, Percent_Identity=41.41689373297, Blast_Score=228, Evalue=4e-61,
Organism=Caenorhabditis elegans, GI17569967, Length=277, Percent_Identity=34.6570397111913, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17569969, Length=277, Percent_Identity=34.6570397111913, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6320506, Length=427, Percent_Identity=33.2552693208431, Blast_Score=197, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6321822, Length=420, Percent_Identity=31.9047619047619, Blast_Score=188, Evalue=1e-48,
Organism=Drosophila melanogaster, GI21357643, Length=305, Percent_Identity=33.4426229508197, Blast_Score=126, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 43009; Mature: 42877

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTCAESRGAPVLPKRSGSRAVTDQRGAICGAATLVVKVGSSSLTLPGGGIDVRRVDDLVD
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHEEEEEEEEECCCEEECCCCCCCHHHHHHHHH
ALSEVIAVGRRVVLVSSGAIATGFPAMGITHRPRTLAGKQAAASVGQGILLAHYASRFAS
HHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCHHCCHHHHHHHCCCHHHHHHHHHHHH
HGLRVGQVLLTVNDLVRPTSYRNAWSTLDTLLGLGVVPIVNENDTVATGEIRFGDNDRLA
CCCCHHHHEEEHHHHHCCCCHHHHHHHHHHHHHCCCEEEECCCCEEEEEEEEECCCCHHH
ALVAELVRAQALILLSDVDALYTAHPDSPDARRVEVVEDIDTLDVDTHKAGSGVGTGGMT
HHHHHHHHHHHEEEEECCHHHEECCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
TKLEAARMATCAGVPVVLAAAVDAPDVLAGVPVGTYFRPLATRRPRRLLWLADAATPQGQ
CHHHHHHHHHHCCCCEEEEECCCCCHHHHCCCCHHHHHHHHCCCCCEEEEEEECCCCCCE
IVIDDGAVEALTQRHSSLLAVGVTRVHGDFQAGDPVTILASDGRVVGRGIAQFSHDEVRV
EEEECCHHHHHHHHHHHEEEEEHEEECCCCCCCCCEEEEECCCEEEECHHHHCCCCHHHE
MRGRSSAWLAAEMGPAASREIIHRDAMVLSRRRKAEPSSRNQKSSGSRVTS
EECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TCAESRGAPVLPKRSGSRAVTDQRGAICGAATLVVKVGSSSLTLPGGGIDVRRVDDLVD
CCCCCCCCCCCCCCCCCCCCCCCCCCHHEEEEEEEEECCCEEECCCCCCCHHHHHHHHH
ALSEVIAVGRRVVLVSSGAIATGFPAMGITHRPRTLAGKQAAASVGQGILLAHYASRFAS
HHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCHHCCHHHHHHHCCCHHHHHHHHHHHH
HGLRVGQVLLTVNDLVRPTSYRNAWSTLDTLLGLGVVPIVNENDTVATGEIRFGDNDRLA
CCCCHHHHEEEHHHHHCCCCHHHHHHHHHHHHHCCCEEEECCCCEEEEEEEEECCCCHHH
ALVAELVRAQALILLSDVDALYTAHPDSPDARRVEVVEDIDTLDVDTHKAGSGVGTGGMT
HHHHHHHHHHHEEEEECCHHHEECCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
TKLEAARMATCAGVPVVLAAAVDAPDVLAGVPVGTYFRPLATRRPRRLLWLADAATPQGQ
CHHHHHHHHHHCCCCEEEEECCCCCHHHHCCCCHHHHHHHHCCCCCEEEEEEECCCCCCE
IVIDDGAVEALTQRHSSLLAVGVTRVHGDFQAGDPVTILASDGRVVGRGIAQFSHDEVRV
EEEECCHHHHHHHHHHHEEEEEHEEECCCCCCCCCEEEEECCCEEEECHHHHCCCCHHHE
MRGRSSAWLAAEMGPAASREIIHRDAMVLSRRRKAEPSSRNQKSSGSRVTS
EECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA