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The map label for this gene is murD

Identifier: 50842243

GI number: 50842243

Start: 831166

End: 832647

Strand: Direct

Name: murD

Synonym: PPA0756

Alternate gene names: 50842243

Gene position: 831166-832647 (Clockwise)

Preceding gene: 50842242

Following gene: 50842244

Centisome position: 32.46

GC content: 61.34

Gene sequence:

>1482_bases
GTGACGTTCTCCGGAAAGGTTGATCTCAGCGCGGCTGACCGGCTCTTCGACTGGTCTGGTGTGCATGTTGTCATCGCTGG
TTTAGGCACGTCCGGCTATGCCGCCGCTGACGCGTTACTTGAGCTTGACGCCACGGTCCTCGTCCTTGACGACTCCGATG
ACGACTCCCACCGTGACAAAGGTGGTCTGCTGGAGGTCCTCGGGGCCGAAGTGCGTTTGGGCCCCGGATCAGCATCCGAC
TTGCCGGAGGAAACGGACCTCGTCGTCGTCTCTCCTGGCTGGCGTCCTACCCAACCGCTGGTAGCTCGTGCCCTTACGCG
AGGGATCCCCGTTTGGGGTGAGCCTGAGCTGGCCTGGCGCCTCATGCATCCCGACCGTGTTATTCCGTGGCTGGCGATTA
CCGGCACCAATGGCAAGACGACGACTACCCAGATGACCGAGTCGATTCTTCATGCGGCAGGTCTGAAGGCATGTGCTGTC
GGTAACATTGGCCGACCCATCCTTGAGGCCATGGCTGACGAGATCAACTACGACGTTTTTGCAGTGGAGCTGTCCAGCTT
CCAGCTGCACTGGTCAAACTCACTGTCTCTCCATTCCGCGGCAGTGCTCAACCTTCATCAAGACCACCTGGAGTGGTACG
CCCACGAATCGGATCCGCTCGGTTCGTACGCCGCCGATAAGGCTCGGATCTACCACCAGGTGACCAACTCCTGCGTCTAC
AACGTTGCCGATCCCGCCACCGAACACATGGTGGAGGATGCCGACGTCGTTGAGGGGGCTCGCGCTATCGGGTTCACGAC
CGGGACCCCGGGGCCGTCCATGATCGGAATTGTGGACGGCCTCATCGTCGATCGTGCCTTCGTTGAGCAGCGCGCGACCT
CGGCGATGGAGCTGGCTCGCCTTGAGGACGTGCACCCTTTCGCTCCGCACAATGTCGAAAACGCGTTGGCTGCTGCGGCA
CTGACCCGCTCCTTTGGTGTTCCTGCGCCTGCCGTCGGACAGGGTTTGCGTGACTTGCATCTTGGTGGACACCGCATCGA
GACGGTGCACCAGGCCGGTGGCATTACCTGGGTCGATGATTCCAAGGCGACGAACCCCCATGCTGCAAACTCCTCGATGC
GGGCCTTTGAACACATCGTGTGGATCGCTGGTGGGCAGGCCAAGGGCACGCATTTTGACAACCTCGTGACCACTCATGCT
GGCAAGCTGCGTGGCGTCGTAGTGCTGGGAACTGACCGCGGCATCATCGCGAGATCTTTGGCCGAGCATGCTCCACAGGT
TCCGGTCGTCGTCATTGACGACATGTCCCGCGAGGCGATGGCTCGTGCGGTCCACGAGGCTGCCAAGATGGCTCGTCCTG
GGGATACCGTCTTGATGGCACCTGGCTGTGCCAGCTTGGATATCTGGCCCGGCTATGCCGCACGCGGTGAGGACTTTGCC
AATGCTGCTCGACACGTTGACGGGCCAGATGCCGATGGCTGA

Upstream 100 bases:

>100_bases
GGTGGGCTGAGGTGACGGTAGTCATCAGGTTCTGGATTATTTGCGGCCTTGCTGTTTCGGCTGGCCTAGGAATCTTCTAT
GCAGAATGGGTGGCAGGGCA

Downstream 100 bases:

>100_bases
GAAATCGGTAGATTCACGGGTCCGCCGTACCACCCGACGCCGGACTCCCGGCAGCTGGTTGAGGCATTTACTGCGACTTG
ACGGTGGTGACGGTTCCTCA

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MTFSGKVDLSAADRLFDWSGVHVVIAGLGTSGYAAADALLELDATVLVLDDSDDDSHRDKGGLLEVLGAEVRLGPGSASD
LPEETDLVVVSPGWRPTQPLVARALTRGIPVWGEPELAWRLMHPDRVIPWLAITGTNGKTTTTQMTESILHAAGLKACAV
GNIGRPILEAMADEINYDVFAVELSSFQLHWSNSLSLHSAAVLNLHQDHLEWYAHESDPLGSYAADKARIYHQVTNSCVY
NVADPATEHMVEDADVVEGARAIGFTTGTPGPSMIGIVDGLIVDRAFVEQRATSAMELARLEDVHPFAPHNVENALAAAA
LTRSFGVPAPAVGQGLRDLHLGGHRIETVHQAGGITWVDDSKATNPHAANSSMRAFEHIVWIAGGQAKGTHFDNLVTTHA
GKLRGVVVLGTDRGIIARSLAEHAPQVPVVVIDDMSREAMARAVHEAAKMARPGDTVLMAPGCASLDIWPGYAARGEDFA
NAARHVDGPDADG

Sequences:

>Translated_493_residues
MTFSGKVDLSAADRLFDWSGVHVVIAGLGTSGYAAADALLELDATVLVLDDSDDDSHRDKGGLLEVLGAEVRLGPGSASD
LPEETDLVVVSPGWRPTQPLVARALTRGIPVWGEPELAWRLMHPDRVIPWLAITGTNGKTTTTQMTESILHAAGLKACAV
GNIGRPILEAMADEINYDVFAVELSSFQLHWSNSLSLHSAAVLNLHQDHLEWYAHESDPLGSYAADKARIYHQVTNSCVY
NVADPATEHMVEDADVVEGARAIGFTTGTPGPSMIGIVDGLIVDRAFVEQRATSAMELARLEDVHPFAPHNVENALAAAA
LTRSFGVPAPAVGQGLRDLHLGGHRIETVHQAGGITWVDDSKATNPHAANSSMRAFEHIVWIAGGQAKGTHFDNLVTTHA
GKLRGVVVLGTDRGIIARSLAEHAPQVPVVVIDDMSREAMARAVHEAAKMARPGDTVLMAPGCASLDIWPGYAARGEDFA
NAARHVDGPDADG
>Mature_492_residues
TFSGKVDLSAADRLFDWSGVHVVIAGLGTSGYAAADALLELDATVLVLDDSDDDSHRDKGGLLEVLGAEVRLGPGSASDL
PEETDLVVVSPGWRPTQPLVARALTRGIPVWGEPELAWRLMHPDRVIPWLAITGTNGKTTTTQMTESILHAAGLKACAVG
NIGRPILEAMADEINYDVFAVELSSFQLHWSNSLSLHSAAVLNLHQDHLEWYAHESDPLGSYAADKARIYHQVTNSCVYN
VADPATEHMVEDADVVEGARAIGFTTGTPGPSMIGIVDGLIVDRAFVEQRATSAMELARLEDVHPFAPHNVENALAAAAL
TRSFGVPAPAVGQGLRDLHLGGHRIETVHQAGGITWVDDSKATNPHAANSSMRAFEHIVWIAGGQAKGTHFDNLVTTHAG
KLRGVVVLGTDRGIIARSLAEHAPQVPVVVIDDMSREAMARAVHEAAKMARPGDTVLMAPGCASLDIWPGYAARGEDFAN
AARHVDGPDADG

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=481, Percent_Identity=30.977130977131, Blast_Score=155, Evalue=4e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_PROAC (Q6A9Q4)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_055470.1
- ProteinModelPortal:   Q6A9Q4
- SMR:   Q6A9Q4
- GeneID:   2932674
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA0756
- NMPDR:   fig|267747.1.peg.747
- HOGENOM:   HBG750024
- OMA:   FQVGRHR
- ProtClustDB:   PRK01438
- BioCyc:   PACN267747:PPA0756-MONOMER
- BRENDA:   6.3.2.9
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 52374; Mature: 52243

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFSGKVDLSAADRLFDWSGVHVVIAGLGTSGYAAADALLELDATVLVLDDSDDDSHRDK
CCCCCCCCCCHHHHCCCCCCCEEEEEECCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCC
GGLLEVLGAEVRLGPGSASDLPEETDLVVVSPGWRPTQPLVARALTRGIPVWGEPELAWR
CCCEEECCCEEEECCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCEEE
LMHPDRVIPWLAITGTNGKTTTTQMTESILHAAGLKACAVGNIGRPILEAMADEINYDVF
EECCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCEEE
AVELSSFQLHWSNSLSLHSAAVLNLHQDHLEWYAHESDPLGSYAADKARIYHQVTNSCVY
EEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHEE
NVADPATEHMVEDADVVEGARAIGFTTGTPGPSMIGIVDGLIVDRAFVEQRATSAMELAR
CCCCCHHHHHHHHHHHHCCCHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEDVHPFAPHNVENALAAAALTRSFGVPAPAVGQGLRDLHLGGHRIETVHQAGGITWVDD
HHCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCHHHHCCCCHHHHHHHCCCEEEECC
SKATNPHAANSSMRAFEHIVWIAGGQAKGTHFDNLVTTHAGKLRGVVVLGTDRGIIARSL
CCCCCCCCCCHHHHHEEEEEEEECCCCCCCCHHHHHHHCCCCEEEEEEEECCCCHHHHHH
AEHAPQVPVVVIDDMSREAMARAVHEAAKMARPGDTVLMAPGCASLDIWPGYAARGEDFA
HHHCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCEECCCCCCCCCCHHH
NAARHVDGPDADG
HHHHCCCCCCCCC
>Mature Secondary Structure 
TFSGKVDLSAADRLFDWSGVHVVIAGLGTSGYAAADALLELDATVLVLDDSDDDSHRDK
CCCCCCCCCHHHHCCCCCCCEEEEEECCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCC
GGLLEVLGAEVRLGPGSASDLPEETDLVVVSPGWRPTQPLVARALTRGIPVWGEPELAWR
CCCEEECCCEEEECCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCEEE
LMHPDRVIPWLAITGTNGKTTTTQMTESILHAAGLKACAVGNIGRPILEAMADEINYDVF
EECCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCEEE
AVELSSFQLHWSNSLSLHSAAVLNLHQDHLEWYAHESDPLGSYAADKARIYHQVTNSCVY
EEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHEE
NVADPATEHMVEDADVVEGARAIGFTTGTPGPSMIGIVDGLIVDRAFVEQRATSAMELAR
CCCCCHHHHHHHHHHHHCCCHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEDVHPFAPHNVENALAAAALTRSFGVPAPAVGQGLRDLHLGGHRIETVHQAGGITWVDD
HHCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCHHHHCCCCHHHHHHHCCCEEEECC
SKATNPHAANSSMRAFEHIVWIAGGQAKGTHFDNLVTTHAGKLRGVVVLGTDRGIIARSL
CCCCCCCCCCHHHHHEEEEEEEECCCCCCCCHHHHHHHCCCCEEEEEEEECCCCHHHHHH
AEHAPQVPVVVIDDMSREAMARAVHEAAKMARPGDTVLMAPGCASLDIWPGYAARGEDFA
HHHCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCEECCCCCCCCCCHHH
NAARHVDGPDADG
HHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA