Click here to switch to the map view.

The map label for this gene is dapE [H]

Identifier: 50842123

GI number: 50842123

Start: 701664

End: 702743

Strand: Direct

Name: dapE [H]

Synonym: PPA0636

Alternate gene names: 50842123

Gene position: 701664-702743 (Clockwise)

Preceding gene: 50842121

Following gene: 50842124

Centisome position: 27.41

GC content: 60.74

Gene sequence:

>1080_bases
ATGGACTTGAAGCCGGCGGGCGACCTGCACGAGCTCTTTCGTCGACTCGTCGACATCGAGTCGGTGTCCATGCACGAGGC
GCCTATTGCCGATGCTGTGGAGGAGACGTTGGCACCACATCGGCATCTTGAGGTGACGAGACTCGGTAACTCTGTCGTTG
CCCGTACTCACCTTGGGCGTTCGGAACGGGTCGTCATTGCTGGTCACCTCGACACCGTTCCGGTCGTCGAGAACTTGCCG
TCCTATATTGAGCAGCGGTCGGACGGGGATTACCTCGTGGGTAGGGGCACGTGCGACATGAAGGGCGGTATCGCCGTCGC
GCTCGCCTTGGCTGTGAGCCTTGAGAATCCGGTGAGGGATGTCACTTGGGCGTTCTATGAGTGTGAGGAGATCGCGGCCG
AGCACAACGGCCTGGAGAAGATTGCCTCTTGGCGCCCTGACCTCATCGACGGTGACTTCGCCATCCTCATGGAACCCACC
GATGGCCGGGTGGAGGGTGGTTGTCAGGGAACCATGCGTTTCACCCTTGAGGTGCCGGGGCGGGCGGCCCATTCAGCTCG
TTCGTGGGTCGGGCACAATGCGATCCATGATCTGTCCGCCGTTTTGGAGCGTCTCAACCGCTGGCAAGAACGGGACCCGT
TTGTTGAGGTTGACGGGCTGACCTTCCGTGAGGGCCTCAATGCGACGATGCTCGACGCCGGTGTGGCCGGTAACGTTATT
CCCCCCACCGCTACTATTCAGATTAATTATCGGTTCGCCCCCGACAAGACGACCGAACAGGCTCGGCAGTACATGGAGGA
TCTCTTCCATGAGTGGCCGATGAACGTTCTTGACCTGTCGGCTCCCGCCAGACCAGGGCTCGATCGTCCGTTGGCGAAGT
CTTTCGTTGCTGCGGTGGGCGGGGAACCGGGTCCTAAGTACGGGTGGACCGACGTTGCCCGTTTCGGGCAACTCGGAATA
CCGGCTGTTAACTTCGGGCCGGGGAATGCGCTGTGCGCTCATCAGGTGGATGAGTGTTGTCGTCTTGGCTCACTGGATGA
GTGCTACCGGGCGCTGCACACGTGGTTAGCGTCGCGATGA

Upstream 100 bases:

>100_bases
ATGCGTAATGTGTCAAGTGCGTTCACCATAGCAATCTCAGGGTCGTCTTTCGCACGTTTTCCTCGGACTGGTCCGTGGCA
ACACTGGTAGGGTGTCCGGC

Downstream 100 bases:

>100_bases
CCTAACCGAGGTTGTCGTCCAATTCTTTCGTTGAGAGGTCTGACATGGCATGGTTACTTGTTGTTGTCGTCATCATCGTC
ATCGGGCTCGCCGTCATGGC

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase [H]

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MDLKPAGDLHELFRRLVDIESVSMHEAPIADAVEETLAPHRHLEVTRLGNSVVARTHLGRSERVVIAGHLDTVPVVENLP
SYIEQRSDGDYLVGRGTCDMKGGIAVALALAVSLENPVRDVTWAFYECEEIAAEHNGLEKIASWRPDLIDGDFAILMEPT
DGRVEGGCQGTMRFTLEVPGRAAHSARSWVGHNAIHDLSAVLERLNRWQERDPFVEVDGLTFREGLNATMLDAGVAGNVI
PPTATIQINYRFAPDKTTEQARQYMEDLFHEWPMNVLDLSAPARPGLDRPLAKSFVAAVGGEPGPKYGWTDVARFGQLGI
PAVNFGPGNALCAHQVDECCRLGSLDECYRALHTWLASR

Sequences:

>Translated_359_residues
MDLKPAGDLHELFRRLVDIESVSMHEAPIADAVEETLAPHRHLEVTRLGNSVVARTHLGRSERVVIAGHLDTVPVVENLP
SYIEQRSDGDYLVGRGTCDMKGGIAVALALAVSLENPVRDVTWAFYECEEIAAEHNGLEKIASWRPDLIDGDFAILMEPT
DGRVEGGCQGTMRFTLEVPGRAAHSARSWVGHNAIHDLSAVLERLNRWQERDPFVEVDGLTFREGLNATMLDAGVAGNVI
PPTATIQINYRFAPDKTTEQARQYMEDLFHEWPMNVLDLSAPARPGLDRPLAKSFVAAVGGEPGPKYGWTDVARFGQLGI
PAVNFGPGNALCAHQVDECCRLGSLDECYRALHTWLASR
>Mature_359_residues
MDLKPAGDLHELFRRLVDIESVSMHEAPIADAVEETLAPHRHLEVTRLGNSVVARTHLGRSERVVIAGHLDTVPVVENLP
SYIEQRSDGDYLVGRGTCDMKGGIAVALALAVSLENPVRDVTWAFYECEEIAAEHNGLEKIASWRPDLIDGDFAILMEPT
DGRVEGGCQGTMRFTLEVPGRAAHSARSWVGHNAIHDLSAVLERLNRWQERDPFVEVDGLTFREGLNATMLDAGVAGNVI
PPTATIQINYRFAPDKTTEQARQYMEDLFHEWPMNVLDLSAPARPGLDRPLAKSFVAAVGGEPGPKYGWTDVARFGQLGI
PAVNFGPGNALCAHQVDECCRLGSLDECYRALHTWLASR

Specific function: Biosynthesis of diaminopimelate and lysine from aspartate semialdehyde; fifth step. [C]

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family [H]

Homologues:

Organism=Escherichia coli, GI1788816, Length=319, Percent_Identity=26.6457680250784, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR002933
- InterPro:   IPR011650
- InterPro:   IPR010174 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 39448; Mature: 39448

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00136 SUBTILASE_ASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLKPAGDLHELFRRLVDIESVSMHEAPIADAVEETLAPHRHLEVTRLGNSVVARTHLGR
CCCCCCCCHHHHHHHHHCHHHHCCCCCCHHHHHHHHHCCCCCEEHHHCCCHHHHHHHCCC
SERVVIAGHLDTVPVVENLPSYIEQRSDGDYLVGRGTCDMKGGIAVALALAVSLENPVRD
CCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCHHH
VTWAFYECEEIAAEHNGLEKIASWRPDLIDGDFAILMEPTDGRVEGGCQGTMRFTLEVPG
HHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEEEECCC
RAAHSARSWVGHNAIHDLSAVLERLNRWQERDPFVEVDGLTFREGLNATMLDAGVAGNVI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEHHCCCCCEEECCCCCCCCC
PPTATIQINYRFAPDKTTEQARQYMEDLFHEWPMNVLDLSAPARPGLDRPLAKSFVAAVG
CCCEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHC
GEPGPKYGWTDVARFGQLGIPAVNFGPGNALCAHQVDECCRLGSLDECYRALHTWLASR
CCCCCCCCHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDLKPAGDLHELFRRLVDIESVSMHEAPIADAVEETLAPHRHLEVTRLGNSVVARTHLGR
CCCCCCCCHHHHHHHHHCHHHHCCCCCCHHHHHHHHHCCCCCEEHHHCCCHHHHHHHCCC
SERVVIAGHLDTVPVVENLPSYIEQRSDGDYLVGRGTCDMKGGIAVALALAVSLENPVRD
CCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCHHH
VTWAFYECEEIAAEHNGLEKIASWRPDLIDGDFAILMEPTDGRVEGGCQGTMRFTLEVPG
HHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEEEECCC
RAAHSARSWVGHNAIHDLSAVLERLNRWQERDPFVEVDGLTFREGLNATMLDAGVAGNVI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEHHCCCCCEEECCCCCCCCC
PPTATIQINYRFAPDKTTEQARQYMEDLFHEWPMNVLDLSAPARPGLDRPLAKSFVAAVG
CCCEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHC
GEPGPKYGWTDVARFGQLGIPAVNFGPGNALCAHQVDECCRLGSLDECYRALHTWLASR
CCCCCCCCHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7881553; 12948626; 7592354 [H]