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The map label for this gene is proP [H]

Identifier: 50842121

GI number: 50842121

Start: 699624

End: 700919

Strand: Direct

Name: proP [H]

Synonym: PPA0634

Alternate gene names: 50842121

Gene position: 699624-700919 (Clockwise)

Preceding gene: 50842120

Following gene: 50842123

Centisome position: 27.33

GC content: 57.95

Gene sequence:

>1296_bases
ATGTCGGAGGACCGCTCGAGGGTCGTCAGGAAGGTCATTGTTGCCTCGTTTCTCGGGAACTTCGTGGAGTGGTTCGACTA
TGCCACGTATTCATATTTGGCAGTGATAATCTCCAGAGTGTTCTTCCCCCAAGGAACAAGTAGGGACGCTCTTGTTCAGA
CATTCGCCATATTCGCCCTGTCCTTCCTACTCCGCCCGATTGGAGCGGTGCTCTGGGGGTGCTGGGGGGACAAATACGGT
CGCAGATGGTCCCTTTCAGTCTCCATCCTCATGATGGCCGTCGCATCCTTCCTTATCGGTTGTTTGCCTTCTTTTGCCGT
GGCAGGGGCGTTCGCGCCCATCGGCCTGCTCGTGTGTCGGATGGTTCAAGGTTTCTCTGCATCCGGGGAGTATGCAGGGG
CGGCCACGTTCTTGGCCGAATATGCACCCGCCTGCCGCCGCGGATGCTACGTGTCCATCGTCCCAGCTTCGACGGCGGCG
GGTCTGCTCATGGGATCCCTGAGCGCTGCGATGATCGCAGGCTGTTTGCCGCAGGGGGCGGTGGGAACGTGGGGATGGCG
GTTGCCTTTTCTGGCGGCCGGACCACTGGGTCTGTTGGCTCGCCACGTGTGGCTCGAGCTCGAGGATTCCCCGGTGTACC
AGGAGCTCAGCGAGAGCGAAACGGGAGCCGCCGTGGGGCTTGGGCCATTGAAGACACTTTTGCGTGAGCACCAGCGATCT
TTATTGGTGAGCATCGGAGTCGCATCGCTTAATGCGGTGGGGTTCTATCTCGTCCTCACTTACCTGCCGACATATCTGTC
GAATGAACTAGGTTTCAGTTCTCGAGCGTCCTTCCTTTCGGTGAATATCAGCCTCTTGTTCTACCTGCTGGCAATCTTCG
TGATGGGTTGGCTCTCTGATCATGTAGGGCGAAAGCGCATGCTGGTTGTGGCCAGTGTGGGCTTCGTCCTTTTTTCGGTT
CCTCTGTTCATCGTCATGGGAACTGGACAGCTGTGGAAGGCATGCCTTGCAGAGGGAGTGTTGTGCCTTTTCCTCACTGC
CAATGATGGCACGATGGCGAGTTACTTGGCCGAGTCGTTCCCGACGGAATTTCGATACACCGGTTTCGCGGTGAGTCAGA
ATCTGGCGAATGCCGTGTTTGGAGGTACGGCGTCTGCCGTTTGTACTTGGGTTATCGGGGTGACTGGTTCACGTTTGGCA
CCCGCGTGGTACCTGACAGCAGTCTCGCTCCTGTCCCTGTGCGCCATGGTCGCTTCGCATGAGCACACGGGGCGTGACCT
CAGTGAGGTCGTCTAG

Upstream 100 bases:

>100_bases
AGGAGGAGCGGGTCCTGTTGTCTTTGAGCTGTTCGGTCGTGATCACCTGCCGAAGGACGAGCGAAGTGTCCCTGACTGCG
GTTGAAATGGGGCTGTGTTG

Downstream 100 bases:

>100_bases
GGGGACTCCGTCTCAGTCCGTTGTGGGCGGGGCTTGTGACATCGGGGCTTTGTGTGTCGGCAGGCTGATCAGCTGGCGAA
GAAGGACTTCGACGGTTGCA

Product: proline/betaine transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MSEDRSRVVRKVIVASFLGNFVEWFDYATYSYLAVIISRVFFPQGTSRDALVQTFAIFALSFLLRPIGAVLWGCWGDKYG
RRWSLSVSILMMAVASFLIGCLPSFAVAGAFAPIGLLVCRMVQGFSASGEYAGAATFLAEYAPACRRGCYVSIVPASTAA
GLLMGSLSAAMIAGCLPQGAVGTWGWRLPFLAAGPLGLLARHVWLELEDSPVYQELSESETGAAVGLGPLKTLLREHQRS
LLVSIGVASLNAVGFYLVLTYLPTYLSNELGFSSRASFLSVNISLLFYLLAIFVMGWLSDHVGRKRMLVVASVGFVLFSV
PLFIVMGTGQLWKACLAEGVLCLFLTANDGTMASYLAESFPTEFRYTGFAVSQNLANAVFGGTASAVCTWVIGVTGSRLA
PAWYLTAVSLLSLCAMVASHEHTGRDLSEVV

Sequences:

>Translated_431_residues
MSEDRSRVVRKVIVASFLGNFVEWFDYATYSYLAVIISRVFFPQGTSRDALVQTFAIFALSFLLRPIGAVLWGCWGDKYG
RRWSLSVSILMMAVASFLIGCLPSFAVAGAFAPIGLLVCRMVQGFSASGEYAGAATFLAEYAPACRRGCYVSIVPASTAA
GLLMGSLSAAMIAGCLPQGAVGTWGWRLPFLAAGPLGLLARHVWLELEDSPVYQELSESETGAAVGLGPLKTLLREHQRS
LLVSIGVASLNAVGFYLVLTYLPTYLSNELGFSSRASFLSVNISLLFYLLAIFVMGWLSDHVGRKRMLVVASVGFVLFSV
PLFIVMGTGQLWKACLAEGVLCLFLTANDGTMASYLAESFPTEFRYTGFAVSQNLANAVFGGTASAVCTWVIGVTGSRLA
PAWYLTAVSLLSLCAMVASHEHTGRDLSEVV
>Mature_430_residues
SEDRSRVVRKVIVASFLGNFVEWFDYATYSYLAVIISRVFFPQGTSRDALVQTFAIFALSFLLRPIGAVLWGCWGDKYGR
RWSLSVSILMMAVASFLIGCLPSFAVAGAFAPIGLLVCRMVQGFSASGEYAGAATFLAEYAPACRRGCYVSIVPASTAAG
LLMGSLSAAMIAGCLPQGAVGTWGWRLPFLAAGPLGLLARHVWLELEDSPVYQELSESETGAAVGLGPLKTLLREHQRSL
LVSIGVASLNAVGFYLVLTYLPTYLSNELGFSSRASFLSVNISLLFYLLAIFVMGWLSDHVGRKRMLVVASVGFVLFSVP
LFIVMGTGQLWKACLAEGVLCLFLTANDGTMASYLAESFPTEFRYTGFAVSQNLANAVFGGTASAVCTWVIGVTGSRLAP
AWYLTAVSLLSLCAMVASHEHTGRDLSEVV

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=426, Percent_Identity=35.2112676056338, Blast_Score=269, Evalue=3e-73,
Organism=Escherichia coli, GI1788942, Length=424, Percent_Identity=32.7830188679245, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1789941, Length=424, Percent_Identity=30.188679245283, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1788292, Length=425, Percent_Identity=29.6470588235294, Blast_Score=168, Evalue=6e-43,
Organism=Escherichia coli, GI87082404, Length=423, Percent_Identity=25.7683215130024, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI87082231, Length=253, Percent_Identity=25.296442687747, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46244; Mature: 46112

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEDRSRVVRKVIVASFLGNFVEWFDYATYSYLAVIISRVFFPQGTSRDALVQTFAIFAL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
SFLLRPIGAVLWGCWGDKYGRRWSLSVSILMMAVASFLIGCLPSFAVAGAFAPIGLLVCR
HHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MVQGFSASGEYAGAATFLAEYAPACRRGCYVSIVPASTAAGLLMGSLSAAMIAGCLPQGA
HHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCC
VGTWGWRLPFLAAGPLGLLARHVWLELEDSPVYQELSESETGAAVGLGPLKTLLREHQRS
CCCCCCCCCHHHCCHHHHHHHHHHEEECCCHHHHHHHCCCCCCEECHHHHHHHHHHHHHH
LLVSIGVASLNAVGFYLVLTYLPTYLSNELGFSSRASFLSVNISLLFYLLAIFVMGWLSD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
HVGRKRMLVVASVGFVLFSVPLFIVMGTGQLWKACLAEGVLCLFLTANDGTMASYLAESF
HCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCEEEEEEECCCCHHHHHHHHHC
PTEFRYTGFAVSQNLANAVFGGTASAVCTWVIGVTGSRLAPAWYLTAVSLLSLCAMVASH
CCCCEECHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCC
EHTGRDLSEVV
CCCCCHHHHCC
>Mature Secondary Structure 
SEDRSRVVRKVIVASFLGNFVEWFDYATYSYLAVIISRVFFPQGTSRDALVQTFAIFAL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
SFLLRPIGAVLWGCWGDKYGRRWSLSVSILMMAVASFLIGCLPSFAVAGAFAPIGLLVCR
HHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MVQGFSASGEYAGAATFLAEYAPACRRGCYVSIVPASTAAGLLMGSLSAAMIAGCLPQGA
HHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCC
VGTWGWRLPFLAAGPLGLLARHVWLELEDSPVYQELSESETGAAVGLGPLKTLLREHQRS
CCCCCCCCCHHHCCHHHHHHHHHHEEECCCHHHHHHHCCCCCCEECHHHHHHHHHHHHHH
LLVSIGVASLNAVGFYLVLTYLPTYLSNELGFSSRASFLSVNISLLFYLLAIFVMGWLSD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
HVGRKRMLVVASVGFVLFSVPLFIVMGTGQLWKACLAEGVLCLFLTANDGTMASYLAESF
HCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCEEEEEEECCCCHHHHHHHHHC
PTEFRYTGFAVSQNLANAVFGGTASAVCTWVIGVTGSRLAPAWYLTAVSLLSLCAMVASH
CCCCEECHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCC
EHTGRDLSEVV
CCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]