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The map label for this gene is mutB [H]

Identifier: 50842082

GI number: 50842082

Start: 659104

End: 661293

Strand: Direct

Name: mutB [H]

Synonym: PPA0596

Alternate gene names: 50842082

Gene position: 659104-661293 (Clockwise)

Preceding gene: 50842081

Following gene: 50842083

Centisome position: 25.74

GC content: 60.91

Gene sequence:

>2190_bases
GTGACCACCCTGCCTCGTTTCGATTCCATTAACCTCGGGGATTCCCCGGTTCCCGCAGATGCGCAGGAGCAGTTCGCCAG
ATTGGCTGCGGCCGCCGGTGAGCAGGAGCCCTGGACCACTCCGGAGCAAATTCCGGTTGGTCACCTCTACTCCGAGGACG
TCTACGGTGACATGGACTGGCTAGACACCTACGCCGGTCTGCCACCATTCACACATGGCCCGTACGCTACCATGTATGCC
TTCCGACCGTGGACGATTCGCCAGTACGCCGGATTCTCCACCGCTAAAGAGTCCAACGCGTTCTACCGTCGTAACCTGGC
CGCCGGTCAGAAGGGCCTGTCGGTCGCCTTCGACCTGCCGACCCACCGCGGCTACGACTCTGACAACCCGCGTGTTCCCG
GCGATGTCGGTATGGCAGGCGTGGCCGTGGACTCCATCCTGGACATGCGTGAGCTCTTTGCGGGCATCCCGCTGGACCGC
ATGTCGGTGTCCATGACGATGAACGGCGCAGTGCTGCCGATTCTTGCCCTGTACGTTGTTACCGCTGAGGAGCAAGGCGC
CAAGCCCGAGCAGCTCGCCGGAACGATCCAGAACGACATCCTCAAGGAGTTCATGGTTCGTAACACCTACATCTACCCGC
CGCTGCCTTCGATGCGGATTATCTCCGACATCTTCGCGTACACCAGCGCGAACATGCCGAAGTGGAACTCCATCTCGATC
TCCGGCTACCACATGCAAGAAGCTGGCGCTACCGCCGATATCGAGATGGCCTACACTCTGGCCGACGGCGTTGATTACAT
TCGCGCCGGTGAGTCGGTGGGCCTACAGGTCGACCAGTTTGCGCCGCGTCTTTCCTTCTTCTGGGCCATTGGCACCAACT
TCTTCATGGAGGTCGCCAAGATGCGTGCGGCCCGCATGCTGTGGGCCAAGCTGGTGCACCAGTTCAACCCGAAGAATCCG
AAGTCGATGAGCTTACGCACTCACTCGCAGACCTCTGGCTGGTCGTTGACAGCCCAGGACGTTTACAACAACGTGATTCG
TACTTGTGTGGAGGCCATGGGAGCCACTCAGGGACACACTCAGTCCCTGCACACGAACTCCCTCGACGAGGCCATCGCTC
TGCCGACCGACTTCTCCGCCCGTATTGCTCGAAACACCCAGCTGTTCATCCAGCAGGAGTCGGGCACCTGCCGCGTTATC
GATCCTTGGAGCGGCTCGGCCTACGTCGAGAAGCTCACCCTCGAGCTGGCTCGCAAGGCCTGGGCTCACATCCAGGAGGT
CGAGAAGGCCGGTGGTATGGCCAAGGCCATTGAGAAGGGCATCCCGAAGATGCGCATCGAGGAAGCTGCTGCTCGTACCC
AGGCTCGTATCGACTCGGGTCGTCAGCCCCTCATCGGCGTCAACAAGTACCGTCTCGACGAGGAGGAGCCCCTCGAGGTC
CTCAAGGTCGACAACACTCAGGTACTCAAGGAACAGAAGGCCAAACTCGAGCAGCTGCGCGCCAACCGCGACGAGGAGGC
GTGCCAGGCCGCTCTGGAGAAGATCACCTGGGCAGCTGCCAACCCGGATCCGAGTGATCCTGACCGTAACCTGCTCAAGC
TGTGCATTGACGCTGGCCGCGCAGATGCGTCTGTCGGTGAGATGTCTGACGCAATGGAGAAGGTCTTCGGGCGTTACACT
GCCCAGATCCGTACCATTGAAGGCGTGTACAGCAAGGCAGCCGGCAATTCTGAGTCCACTAAGAAGGTCCACGAGCTCAT
CAAGCAGTTCGAGGAGAAGGAAGGCCGTCGTCCGCGTATCATGATCGCGAAGATGGGCCAGGACGGTCACGACCGTGGCC
AGAAGGTCGTCGCGACCGCTTATGCTGACCTTGGTATGGACGTTGATGTCGGCCCGCTGTTCCAGACCCCGGAGGAGACT
GCTCGACAGGCTGTTGAGGGTGACGTCCACGTTGTCGGTGTCTCCTCACTGGCCGCCGGGCACCTTACGCTAGTGCCGGC
CCTGCGTAAGGAACTGGACAAGCTTGGTCGCTCCGACATCATGATTGTCGTCGGTGGCGTCATTCCGACCCAGGACTTCG
ACGAGCTGCGCAAGGATGGCGCGGCGGCCATCTATCCGCCTGGCACCGTTATCCCGGACGCTGCCGTTGAGTTGATGGAG
AAGCTGCTCGCCGCGCACAACGACGACTGA

Upstream 100 bases:

>100_bases
ATGCCGCCGAGGCCGAGAAGGTCATCGACGGGCGCATCTACCTCGGGATGGACGTCGTGGACGTCCTGACCGCCACTCTG
GACACGTTGGGAGTTGCCAA

Downstream 100 bases:

>100_bases
GCCCGATAGGTTGCTAACCTGAACGAGGCCTTCGGAGCCTAGGTTTCGAAGGCCTCGTGAGCTCCCGATGTTGGGATACT
ACGGAAAACCACGCGGGATG

Product: methylmalonyl-CoA mutase

Products: NA

Alternate protein names: MCM-alpha [H]

Number of amino acids: Translated: 729; Mature: 728

Protein sequence:

>729_residues
MTTLPRFDSINLGDSPVPADAQEQFARLAAAAGEQEPWTTPEQIPVGHLYSEDVYGDMDWLDTYAGLPPFTHGPYATMYA
FRPWTIRQYAGFSTAKESNAFYRRNLAAGQKGLSVAFDLPTHRGYDSDNPRVPGDVGMAGVAVDSILDMRELFAGIPLDR
MSVSMTMNGAVLPILALYVVTAEEQGAKPEQLAGTIQNDILKEFMVRNTYIYPPLPSMRIISDIFAYTSANMPKWNSISI
SGYHMQEAGATADIEMAYTLADGVDYIRAGESVGLQVDQFAPRLSFFWAIGTNFFMEVAKMRAARMLWAKLVHQFNPKNP
KSMSLRTHSQTSGWSLTAQDVYNNVIRTCVEAMGATQGHTQSLHTNSLDEAIALPTDFSARIARNTQLFIQQESGTCRVI
DPWSGSAYVEKLTLELARKAWAHIQEVEKAGGMAKAIEKGIPKMRIEEAAARTQARIDSGRQPLIGVNKYRLDEEEPLEV
LKVDNTQVLKEQKAKLEQLRANRDEEACQAALEKITWAAANPDPSDPDRNLLKLCIDAGRADASVGEMSDAMEKVFGRYT
AQIRTIEGVYSKAAGNSESTKKVHELIKQFEEKEGRRPRIMIAKMGQDGHDRGQKVVATAYADLGMDVDVGPLFQTPEET
ARQAVEGDVHVVGVSSLAAGHLTLVPALRKELDKLGRSDIMIVVGGVIPTQDFDELRKDGAAAIYPPGTVIPDAAVELME
KLLAAHNDD

Sequences:

>Translated_729_residues
MTTLPRFDSINLGDSPVPADAQEQFARLAAAAGEQEPWTTPEQIPVGHLYSEDVYGDMDWLDTYAGLPPFTHGPYATMYA
FRPWTIRQYAGFSTAKESNAFYRRNLAAGQKGLSVAFDLPTHRGYDSDNPRVPGDVGMAGVAVDSILDMRELFAGIPLDR
MSVSMTMNGAVLPILALYVVTAEEQGAKPEQLAGTIQNDILKEFMVRNTYIYPPLPSMRIISDIFAYTSANMPKWNSISI
SGYHMQEAGATADIEMAYTLADGVDYIRAGESVGLQVDQFAPRLSFFWAIGTNFFMEVAKMRAARMLWAKLVHQFNPKNP
KSMSLRTHSQTSGWSLTAQDVYNNVIRTCVEAMGATQGHTQSLHTNSLDEAIALPTDFSARIARNTQLFIQQESGTCRVI
DPWSGSAYVEKLTLELARKAWAHIQEVEKAGGMAKAIEKGIPKMRIEEAAARTQARIDSGRQPLIGVNKYRLDEEEPLEV
LKVDNTQVLKEQKAKLEQLRANRDEEACQAALEKITWAAANPDPSDPDRNLLKLCIDAGRADASVGEMSDAMEKVFGRYT
AQIRTIEGVYSKAAGNSESTKKVHELIKQFEEKEGRRPRIMIAKMGQDGHDRGQKVVATAYADLGMDVDVGPLFQTPEET
ARQAVEGDVHVVGVSSLAAGHLTLVPALRKELDKLGRSDIMIVVGGVIPTQDFDELRKDGAAAIYPPGTVIPDAAVELME
KLLAAHNDD
>Mature_728_residues
TTLPRFDSINLGDSPVPADAQEQFARLAAAAGEQEPWTTPEQIPVGHLYSEDVYGDMDWLDTYAGLPPFTHGPYATMYAF
RPWTIRQYAGFSTAKESNAFYRRNLAAGQKGLSVAFDLPTHRGYDSDNPRVPGDVGMAGVAVDSILDMRELFAGIPLDRM
SVSMTMNGAVLPILALYVVTAEEQGAKPEQLAGTIQNDILKEFMVRNTYIYPPLPSMRIISDIFAYTSANMPKWNSISIS
GYHMQEAGATADIEMAYTLADGVDYIRAGESVGLQVDQFAPRLSFFWAIGTNFFMEVAKMRAARMLWAKLVHQFNPKNPK
SMSLRTHSQTSGWSLTAQDVYNNVIRTCVEAMGATQGHTQSLHTNSLDEAIALPTDFSARIARNTQLFIQQESGTCRVID
PWSGSAYVEKLTLELARKAWAHIQEVEKAGGMAKAIEKGIPKMRIEEAAARTQARIDSGRQPLIGVNKYRLDEEEPLEVL
KVDNTQVLKEQKAKLEQLRANRDEEACQAALEKITWAAANPDPSDPDRNLLKLCIDAGRADASVGEMSDAMEKVFGRYTA
QIRTIEGVYSKAAGNSESTKKVHELIKQFEEKEGRRPRIMIAKMGQDGHDRGQKVVATAYADLGMDVDVGPLFQTPEETA
RQAVEGDVHVVGVSSLAAGHLTLVPALRKELDKLGRSDIMIVVGGVIPTQDFDELRKDGAAAIYPPGTVIPDAAVELMEK
LLAAHNDD

Specific function: Catalyzes the isomerization of succinyl-CoA to methylmalonyl-CoA during synthesis of propionate from tricarboxylic acid-cycle intermediates [H]

COG id: COG1884

COG function: function code I; Methylmalonyl-CoA mutase, N-terminal domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156105689, Length=686, Percent_Identity=60.2040816326531, Blast_Score=863, Evalue=0.0,
Organism=Escherichia coli, GI1789284, Length=693, Percent_Identity=59.7402597402597, Blast_Score=832, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25144500, Length=689, Percent_Identity=63.5703918722787, Blast_Score=894, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006159
- InterPro:   IPR016176
- InterPro:   IPR014348
- InterPro:   IPR006158
- InterPro:   IPR006099
- InterPro:   IPR006098 [H]

Pfam domain/function: PF02310 B12-binding; PF01642 MM_CoA_mutase [H]

EC number: =5.4.99.2 [H]

Molecular weight: Translated: 80179; Mature: 80048

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00544 METMALONYL_COA_MUTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLPRFDSINLGDSPVPADAQEQFARLAAAAGEQEPWTTPEQIPVGHLYSEDVYGDMDW
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCCCCHHH
LDTYAGLPPFTHGPYATMYAFRPWTIRQYAGFSTAKESNAFYRRNLAAGQKGLSVAFDLP
HHHHCCCCCCCCCCCEEEEEECCEEHHHHCCCCCCHHCCHHHHHHHHCCCCCCEEEEECC
THRGYDSDNPRVPGDVGMAGVAVDSILDMRELFAGIPLDRMSVSMTMNGAVLPILALYVV
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHEEEEEEECCHHHHHHHHHHH
TAEEQGAKPEQLAGTIQNDILKEFMVRNTYIYPPLPSMRIISDIFAYTSANMPKWNSISI
CCHHCCCCHHHHHHHHHHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHCCCCCCCCEEEE
SGYHMQEAGATADIEMAYTLADGVDYIRAGESVGLQVDQFAPRLSFFWAIGTNFFMEVAK
ECEEECCCCCCCCEEEEEEHHHCHHHHHCCCCCCEEHHHHCCHHHHHHHHHHHHHHHHHH
MRAARMLWAKLVHQFNPKNPKSMSLRTHSQTSGWSLTAQDVYNNVIRTCVEAMGATQGHT
HHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCC
QSLHTNSLDEAIALPTDFSARIARNTQLFIQQESGTCRVIDPWSGSAYVEKLTLELARKA
CCCCCCCCHHHHCCCCCCHHHHHCCEEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHH
WAHIQEVEKAGGMAKAIEKGIPKMRIEEAAARTQARIDSGRQPLIGVNKYRLDEEEPLEV
HHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCE
LKVDNTQVLKEQKAKLEQLRANRDEEACQAALEKITWAAANPDPSDPDRNLLKLCIDAGR
EECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHCCC
ADASVGEMSDAMEKVFGRYTAQIRTIEGVYSKAAGNSESTKKVHELIKQFEEKEGRRPRI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEE
MIAKMGQDGHDRGQKVVATAYADLGMDVDVGPLFQTPEETARQAVEGDVHVVGVSSLAAG
EEEECCCCCCHHCCEEEEEHHHHCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEHHHHCCC
HLTLVPALRKELDKLGRSDIMIVVGGVIPTQDFDELRKDGAAAIYPPGTVIPDAAVELME
HHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHCCCEEECCCCCCCCHHHHHHHH
KLLAAHNDD
HHHHHCCCC
>Mature Secondary Structure 
TTLPRFDSINLGDSPVPADAQEQFARLAAAAGEQEPWTTPEQIPVGHLYSEDVYGDMDW
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCCCCHHH
LDTYAGLPPFTHGPYATMYAFRPWTIRQYAGFSTAKESNAFYRRNLAAGQKGLSVAFDLP
HHHHCCCCCCCCCCCEEEEEECCEEHHHHCCCCCCHHCCHHHHHHHHCCCCCCEEEEECC
THRGYDSDNPRVPGDVGMAGVAVDSILDMRELFAGIPLDRMSVSMTMNGAVLPILALYVV
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHEEEEEEECCHHHHHHHHHHH
TAEEQGAKPEQLAGTIQNDILKEFMVRNTYIYPPLPSMRIISDIFAYTSANMPKWNSISI
CCHHCCCCHHHHHHHHHHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHCCCCCCCCEEEE
SGYHMQEAGATADIEMAYTLADGVDYIRAGESVGLQVDQFAPRLSFFWAIGTNFFMEVAK
ECEEECCCCCCCCEEEEEEHHHCHHHHHCCCCCCEEHHHHCCHHHHHHHHHHHHHHHHHH
MRAARMLWAKLVHQFNPKNPKSMSLRTHSQTSGWSLTAQDVYNNVIRTCVEAMGATQGHT
HHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCC
QSLHTNSLDEAIALPTDFSARIARNTQLFIQQESGTCRVIDPWSGSAYVEKLTLELARKA
CCCCCCCCHHHHCCCCCCHHHHHCCEEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHH
WAHIQEVEKAGGMAKAIEKGIPKMRIEEAAARTQARIDSGRQPLIGVNKYRLDEEEPLEV
HHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCE
LKVDNTQVLKEQKAKLEQLRANRDEEACQAALEKITWAAANPDPSDPDRNLLKLCIDAGR
EECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHCCC
ADASVGEMSDAMEKVFGRYTAQIRTIEGVYSKAAGNSESTKKVHELIKQFEEKEGRRPRI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEE
MIAKMGQDGHDRGQKVVATAYADLGMDVDVGPLFQTPEETARQAVEGDVHVVGVSSLAAG
EEEECCCCCCHHCCEEEEEHHHHCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEHHHHCCC
HLTLVPALRKELDKLGRSDIMIVVGGVIPTQDFDELRKDGAAAIYPPGTVIPDAAVELME
HHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHCCCEEECCCCCCCCHHHHHHHH
KLLAAHNDD
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2569861; 2569860; 8805541; 9655823 [H]