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The map label for this gene is tilS

Identifier: 50841748

GI number: 50841748

Start: 311745

End: 312680

Strand: Direct

Name: tilS

Synonym: PPA0258

Alternate gene names: 50841748

Gene position: 311745-312680 (Clockwise)

Preceding gene: 50841747

Following gene: 50841749

Centisome position: 12.18

GC content: 67.63

Gene sequence:

>936_bases
ATGGCACGACGGGAATTAGGGCCAGCGGCCCTGGCAGTCGCCCAGGCCGTCGAGCGGATGGTGCGAGGGGTTGATCGGTT
CGTCGTCGGGTGCTCGGGCGGTCCGGACTCGCTGGCCTTGGCTTTGGCCTCAAAGTGGGCCTCGCGGCGGTGCGAATCTG
GCGTGAGGGTCGTCATTGTCGACCATCAATTGCAGCAGGGGTCCGACGAAGTGGCCGAGCGGACCCGGGACTTGCTGGTG
CGACGCGGCATGGATGCATGTGTGCGTCGCGTCGACGTTGACGCTGATGACCCCGATGGCCCCGAGGCGGCGGCGCGGAC
TGCTCGTCGGGCAGCACTGCTCGACGTCGCTGGGGATGAGCCGGTGTTGCTCGGGCACACCCGGGACGATCAGGCCGAGC
AGGTGTTGCTTTCTCTGGCCAGGGGCTCCGGGGCGACGTCGCTGGCAGGTATTCGGCCGCGTTCCGGTCAGTTTTGGCAT
CCGCTGCTAGAGGTGCGACGGGCTCAGACGGTGCAGGCGTGCCGGGAGTGGGAGGTTGAGCCGTGGCAGGACCCTCACAA
TGGTGATCCACGGTTCCTGCGCTCTCGGGTGCGCACTGAGCTCATGCCGGTTATGGAGGAGGTGCTGGGCCCAGAGGTGG
CGGCATCCCTGGCTCGCAGCGCGACCTTGCTGGCAGGGGAAGATGAGGTCGTCGCCCGGGTTGCGCGGATGTGGGCGGAT
GAGCACGGGGTGAAGGCCAATGAATTGCCCGGTTTGCGTGGGGTTGAGGTCGGCCTGGCGCGCCGCGTCGTCAAGGAGTG
GCTGCCGCAGGCGAGGATGGTGCACGTCGATGCCGTTTTGGGCTTGCTCGACGGGCCTGGTGGTGCAGGGGTGGACGTCC
CGGGCGGACGCGTCGAGATGAGACAGGGGACTCTGTATCTAGCCCGGCGCCTGTGA

Upstream 100 bases:

>100_bases
TTTTGGTGGCTTCCTTCATCATCAATCAGACTGACGGCAGTGCCCGGTCGTGGCTCGACGTCGTTTACGGCAACCTGGCC
GGGTGCGGCTGCACCCGCTG

Downstream 100 bases:

>100_bases
TGGGACATAAGTCGGTAGGCTGATGGTGTGCGAGCTTCTGACATTCCTGGAGATTTCGACCACGTTCTGTACACCGAGGA
GCAGGTGGCCTCCCGTATTC

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MARRELGPAALAVAQAVERMVRGVDRFVVGCSGGPDSLALALASKWASRRCESGVRVVIVDHQLQQGSDEVAERTRDLLV
RRGMDACVRRVDVDADDPDGPEAAARTARRAALLDVAGDEPVLLGHTRDDQAEQVLLSLARGSGATSLAGIRPRSGQFWH
PLLEVRRAQTVQACREWEVEPWQDPHNGDPRFLRSRVRTELMPVMEEVLGPEVAASLARSATLLAGEDEVVARVARMWAD
EHGVKANELPGLRGVEVGLARRVVKEWLPQARMVHVDAVLGLLDGPGGAGVDVPGGRVEMRQGTLYLARRL

Sequences:

>Translated_311_residues
MARRELGPAALAVAQAVERMVRGVDRFVVGCSGGPDSLALALASKWASRRCESGVRVVIVDHQLQQGSDEVAERTRDLLV
RRGMDACVRRVDVDADDPDGPEAAARTARRAALLDVAGDEPVLLGHTRDDQAEQVLLSLARGSGATSLAGIRPRSGQFWH
PLLEVRRAQTVQACREWEVEPWQDPHNGDPRFLRSRVRTELMPVMEEVLGPEVAASLARSATLLAGEDEVVARVARMWAD
EHGVKANELPGLRGVEVGLARRVVKEWLPQARMVHVDAVLGLLDGPGGAGVDVPGGRVEMRQGTLYLARRL
>Mature_310_residues
ARRELGPAALAVAQAVERMVRGVDRFVVGCSGGPDSLALALASKWASRRCESGVRVVIVDHQLQQGSDEVAERTRDLLVR
RGMDACVRRVDVDADDPDGPEAAARTARRAALLDVAGDEPVLLGHTRDDQAEQVLLSLARGSGATSLAGIRPRSGQFWHP
LLEVRRAQTVQACREWEVEPWQDPHNGDPRFLRSRVRTELMPVMEEVLGPEVAASLARSATLLAGEDEVVARVARMWADE
HGVKANELPGLRGVEVGLARRVVKEWLPQARMVHVDAVLGLLDGPGGAGVDVPGGRVEMRQGTLYLARRL

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=219, Percent_Identity=27.8538812785388, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_PROAC (Q6AB49)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_054975.1
- ProteinModelPortal:   Q6AB49
- SMR:   Q6AB49
- GeneID:   2931259
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA0258
- NMPDR:   fig|267747.1.peg.252
- HOGENOM:   HBG692699
- OMA:   ETNENTH
- ProtClustDB:   CLSK2763167
- BioCyc:   PACN267747:PPA0258-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3

EC number: NA

Molecular weight: Translated: 33758; Mature: 33627

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARRELGPAALAVAQAVERMVRGVDRFVVGCSGGPDSLALALASKWASRRCESGVRVVIV
CCCCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEEE
DHQLQQGSDEVAERTRDLLVRRGMDACVRRVDVDADDPDGPEAAARTARRAALLDVAGDE
CCCHHCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHEECCCC
PVLLGHTRDDQAEQVLLSLARGSGATSLAGIRPRSGQFWHPLLEVRRAQTVQACREWEVE
CEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
PWQDPHNGDPRFLRSRVRTELMPVMEEVLGPEVAASLARSATLLAGEDEVVARVARMWAD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
EHGVKANELPGLRGVEVGLARRVVKEWLPQARMVHVDAVLGLLDGPGGAGVDVPGGRVEM
HCCCCHHCCCCCCCHHHHHHHHHHHHHCCHHHEEHHHHHHHHHCCCCCCCCCCCCCEEEE
RQGTLYLARRL
ECCHHHHHHCC
>Mature Secondary Structure 
ARRELGPAALAVAQAVERMVRGVDRFVVGCSGGPDSLALALASKWASRRCESGVRVVIV
CCCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEEE
DHQLQQGSDEVAERTRDLLVRRGMDACVRRVDVDADDPDGPEAAARTARRAALLDVAGDE
CCCHHCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHEECCCC
PVLLGHTRDDQAEQVLLSLARGSGATSLAGIRPRSGQFWHPLLEVRRAQTVQACREWEVE
CEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
PWQDPHNGDPRFLRSRVRTELMPVMEEVLGPEVAASLARSATLLAGEDEVVARVARMWAD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
EHGVKANELPGLRGVEVGLARRVVKEWLPQARMVHVDAVLGLLDGPGGAGVDVPGGRVEM
HCCCCHHCCCCCCCHHHHHHHHHHHHHCCHHHEEHHHHHHHHHCCCCCCCCCCCCCEEEE
RQGTLYLARRL
ECCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA