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The map label for this gene is glcA [H]

Identifier: 50841654

GI number: 50841654

Start: 199902

End: 201590

Strand: Direct

Name: glcA [H]

Synonym: PPA0166

Alternate gene names: 50841654

Gene position: 199902-201590 (Clockwise)

Preceding gene: 50841652

Following gene: 50841655

Centisome position: 7.81

GC content: 61.57

Gene sequence:

>1689_bases
ATGGTTCTTCTCCAGACCTTCACCCCATCGGCCAACCCTCTAGGGTCGCAATGGCTGTCGGCCCTCTTGGCGGTCCTGCC
GGTGCTGGCCATGCTCATTACCCTTGGTGCGCTGCGTTGGAAGGCGCACCTGGCTGGCCTCTTCTCATGGGGTGTCGCCC
TCATTGTGGCGATCACCGCCTTCCCTATGCCGATCGGAATGGCGTTTTCCTCAAGCGTCCAAGGTTTCCTCTACGGCCTC
TTCCCGATTTCGTGGATCCTGCTAGGCGCCATCTGGATGTATCAGGTGACGGTCATTTCCGGTCGCTTCGACGACCTGCG
CCGCACCTTCTTCCTCATCAGCGACGACCCGCGTGTGCTGGGCATCCTCATCGCCTTCTGCTTCGGTGGTCTACTAGAAG
CCCTGGCCGGGTTCGGTGCCCCCGTCGCCATCGCCGCTGCGATGCTCATCGCTATCGGATTCGGCAAGCTCCGTGGTGCC
ATCACTGCCTTGGTCGCTAATACCGTCCCGGTGGCGTTCGGAGCCGTCGGTCTGCCGGTTCTCATGGCCGCCAAGACCGG
TGGCTTGGACGTCGCGATGGTGGCCCCGGTCACCGCCCGCGTCTGCGCCGTGCTCTGCCTCGTCGTCCCCTTCCTCATGC
TGGCGATCATGGACGGCCGTAAGGGTGTTCGCGAATGCTGGCCCTTTGGCCTGTTCGTCGGCGTCGTCTTCGGTGTGACG
AAGGTCATCGTCGGCGGCACCCCGGTCTACAACCTCACTGAGATTTTCGCCGCCGTCGTCACCGTCGCCCTCTCCCTGCT
TTTCCTTAAAGTCTGGAAACCCAAGGGCGGCCGCGAGGCCGCCGAGCGCATCGGCATCCCAATCGATCCGACCGTCGAGA
TCGAGTCGGTCGAGGCTCAGGAGGTCGACACCTCTGACCTCACTGGCAGCCGTATCTTTATGGCCCTCGTTCCCTACATC
CTCGTCATCGTGGTCTTCGGTATCGCCGCTATCCCTGCTGTCAAGGACAGTTTCAAAGCCGCCGACGTGAAGATGGCCTG
GCCTGGCCTATCCGGACTCATGACCGCCGGTGGCGAGGCCGCCGCCCACCAGACATACACCTGGCAATGGCTGTCCCAGC
CGGGCTTCTTGCTTGCCATCGTCGCAATTCTCGTGGGCTTCATCTACCGGGTGCCCCTCGCCGACGTCACTAAAGAGCTA
TGGATCAACGCCAAGAAGATGAAGTTTTCGATGCTGACCATCGGTTTGGTCGTCGCTTTGGCCTATGTGATGGGTGACTC
CGGCCAGACCCTTGCTCTTGGCATGTTCATCGCTGGGGCAGGAGCGGTCTACCCGTTCCTCGCCCCGATCCTCGGTTGGA
TCGGCACGTACGTCACCGGATCCGATACCTCCGCCAACATCCTGTTCTCTGGCTTACAGAGCAGTGTGGGACAACAGGTC
GGTGCTGGGTCATATCTTGGTATGGAAAGCATGCGGCATTTGCTGGTTGGTGCGGGCGCATCCGGCGGTGTCGTCGGCAA
GATGATCTCCCCGCAGTCGCTGACCATCGCGGCCACCGCGATAGGTCTAGCCGGCGGCGAGTCGGTCATCCTGCGCAAGG
TTTTTAAATTCTCGATCATCCTCCTCGTCCTGATGTGCATCATCGTCGGCCTCATGTCGACCCCGGTGCTCGGATGGATG
GTCCCCTGA

Upstream 100 bases:

>100_bases
ATCGAAAACGGTCCTACCGTCATATTGGTCGGACCAAATCGGTCCGACCTTGCCAGCGGTTAGCCGACCGTAGGCAGTGG
TTGAACAACGGAGTTCTTCG

Downstream 100 bases:

>100_bases
ATCTGCTGACGGTTGACACCGCCTGGCATCCGTTCATTATCAATGGAGGTCACCATGACAACCACTACAAGCCCTGGCGC
CAGGGCTACCTTGCCCGGCT

Product: putative L-lactate permease

Products: lactate [Cytoplasm]; Proton [Cytoplasm]; glycolate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 562; Mature: 562

Protein sequence:

>562_residues
MVLLQTFTPSANPLGSQWLSALLAVLPVLAMLITLGALRWKAHLAGLFSWGVALIVAITAFPMPIGMAFSSSVQGFLYGL
FPISWILLGAIWMYQVTVISGRFDDLRRTFFLISDDPRVLGILIAFCFGGLLEALAGFGAPVAIAAAMLIAIGFGKLRGA
ITALVANTVPVAFGAVGLPVLMAAKTGGLDVAMVAPVTARVCAVLCLVVPFLMLAIMDGRKGVRECWPFGLFVGVVFGVT
KVIVGGTPVYNLTEIFAAVVTVALSLLFLKVWKPKGGREAAERIGIPIDPTVEIESVEAQEVDTSDLTGSRIFMALVPYI
LVIVVFGIAAIPAVKDSFKAADVKMAWPGLSGLMTAGGEAAAHQTYTWQWLSQPGFLLAIVAILVGFIYRVPLADVTKEL
WINAKKMKFSMLTIGLVVALAYVMGDSGQTLALGMFIAGAGAVYPFLAPILGWIGTYVTGSDTSANILFSGLQSSVGQQV
GAGSYLGMESMRHLLVGAGASGGVVGKMISPQSLTIAATAIGLAGGESVILRKVFKFSIILLVLMCIIVGLMSTPVLGWM
VP

Sequences:

>Translated_562_residues
MVLLQTFTPSANPLGSQWLSALLAVLPVLAMLITLGALRWKAHLAGLFSWGVALIVAITAFPMPIGMAFSSSVQGFLYGL
FPISWILLGAIWMYQVTVISGRFDDLRRTFFLISDDPRVLGILIAFCFGGLLEALAGFGAPVAIAAAMLIAIGFGKLRGA
ITALVANTVPVAFGAVGLPVLMAAKTGGLDVAMVAPVTARVCAVLCLVVPFLMLAIMDGRKGVRECWPFGLFVGVVFGVT
KVIVGGTPVYNLTEIFAAVVTVALSLLFLKVWKPKGGREAAERIGIPIDPTVEIESVEAQEVDTSDLTGSRIFMALVPYI
LVIVVFGIAAIPAVKDSFKAADVKMAWPGLSGLMTAGGEAAAHQTYTWQWLSQPGFLLAIVAILVGFIYRVPLADVTKEL
WINAKKMKFSMLTIGLVVALAYVMGDSGQTLALGMFIAGAGAVYPFLAPILGWIGTYVTGSDTSANILFSGLQSSVGQQV
GAGSYLGMESMRHLLVGAGASGGVVGKMISPQSLTIAATAIGLAGGESVILRKVFKFSIILLVLMCIIVGLMSTPVLGWM
VP
>Mature_562_residues
MVLLQTFTPSANPLGSQWLSALLAVLPVLAMLITLGALRWKAHLAGLFSWGVALIVAITAFPMPIGMAFSSSVQGFLYGL
FPISWILLGAIWMYQVTVISGRFDDLRRTFFLISDDPRVLGILIAFCFGGLLEALAGFGAPVAIAAAMLIAIGFGKLRGA
ITALVANTVPVAFGAVGLPVLMAAKTGGLDVAMVAPVTARVCAVLCLVVPFLMLAIMDGRKGVRECWPFGLFVGVVFGVT
KVIVGGTPVYNLTEIFAAVVTVALSLLFLKVWKPKGGREAAERIGIPIDPTVEIESVEAQEVDTSDLTGSRIFMALVPYI
LVIVVFGIAAIPAVKDSFKAADVKMAWPGLSGLMTAGGEAAAHQTYTWQWLSQPGFLLAIVAILVGFIYRVPLADVTKEL
WINAKKMKFSMLTIGLVVALAYVMGDSGQTLALGMFIAGAGAVYPFLAPILGWIGTYVTGSDTSANILFSGLQSSVGQQV
GAGSYLGMESMRHLLVGAGASGGVVGKMISPQSLTIAATAIGLAGGESVILRKVFKFSIILLVLMCIIVGLMSTPVLGWM
VP

Specific function: Transports glycolate across the membrane. Can also transport L-lactate and D-lactate. Seems to be driven by a proton motive force [H]

COG id: COG1620

COG function: function code C; L-lactate permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lactate permease family [H]

Homologues:

Organism=Escherichia coli, GI1789347, Length=570, Percent_Identity=37.5438596491228, Blast_Score=319, Evalue=3e-88,
Organism=Escherichia coli, GI1790031, Length=568, Percent_Identity=33.4507042253521, Blast_Score=291, Evalue=6e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003804 [H]

Pfam domain/function: PF02652 Lactate_perm [H]

EC number: NA

Molecular weight: Translated: 58966; Mature: 58966

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLLQTFTPSANPLGSQWLSALLAVLPVLAMLITLGALRWKAHLAGLFSWGVALIVAITA
CEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FPMPIGMAFSSSVQGFLYGLFPISWILLGAIWMYQVTVISGRFDDLRRTFFLISDDPRVL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHEEEEECCCCHHH
GILIAFCFGGLLEALAGFGAPVAIAAAMLIAIGFGKLRGAITALVANTVPVAFGAVGLPV
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMAAKTGGLDVAMVAPVTARVCAVLCLVVPFLMLAIMDGRKGVRECWPFGLFVGVVFGVT
HHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
KVIVGGTPVYNLTEIFAAVVTVALSLLFLKVWKPKGGREAAERIGIPIDPTVEIESVEAQ
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCEEEECCCHH
EVDTSDLTGSRIFMALVPYILVIVVFGIAAIPAVKDSFKAADVKMAWPGLSGLMTAGGEA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHCCCCH
AAHQTYTWQWLSQPGFLLAIVAILVGFIYRVPLADVTKELWINAKKMKFSMLTIGLVVAL
HHCCCEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
AYVMGDSGQTLALGMFIAGAGAVYPFLAPILGWIGTYVTGSDTSANILFSGLQSSVGQQV
HHHHCCCCCEEEHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHC
GAGSYLGMESMRHLLVGAGASGGVVGKMISPQSLTIAATAIGLAGGESVILRKVFKFSII
CCCCHHHHHHHHHHHHCCCCCCCCEECCCCCCCEEEEHHHHHCCCCHHHHHHHHHHHHHH
LLVLMCIIVGLMSTPVLGWMVP
HHHHHHHHHHHHHCCCHHCCCC
>Mature Secondary Structure
MVLLQTFTPSANPLGSQWLSALLAVLPVLAMLITLGALRWKAHLAGLFSWGVALIVAITA
CEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FPMPIGMAFSSSVQGFLYGLFPISWILLGAIWMYQVTVISGRFDDLRRTFFLISDDPRVL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHEEEEECCCCHHH
GILIAFCFGGLLEALAGFGAPVAIAAAMLIAIGFGKLRGAITALVANTVPVAFGAVGLPV
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMAAKTGGLDVAMVAPVTARVCAVLCLVVPFLMLAIMDGRKGVRECWPFGLFVGVVFGVT
HHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
KVIVGGTPVYNLTEIFAAVVTVALSLLFLKVWKPKGGREAAERIGIPIDPTVEIESVEAQ
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCEEEECCCHH
EVDTSDLTGSRIFMALVPYILVIVVFGIAAIPAVKDSFKAADVKMAWPGLSGLMTAGGEA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHCCCCH
AAHQTYTWQWLSQPGFLLAIVAILVGFIYRVPLADVTKELWINAKKMKFSMLTIGLVVAL
HHCCCEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
AYVMGDSGQTLALGMFIAGAGAVYPFLAPILGWIGTYVTGSDTSANILFSGLQSSVGQQV
HHHHCCCCCEEEHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHC
GAGSYLGMESMRHLLVGAGASGGVVGKMISPQSLTIAATAIGLAGGESVILRKVFKFSII
CCCCHHHHHHHHHHHHCCCCCCCCEECCCCCCCEEEEHHHHHCCCCHHHHHHHHHHHHHH
LLVLMCIIVGLMSTPVLGWMVP
HHHHHHHHHHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lactate [Periplasm]; Proton [Periplasm]; glycolate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + lactate [Periplasm] = Proton [Cytoplasm] + lactate [Cytoplasm] Proton [Periplasm] + glycolate [Periplasm] = Proton [Cytoplasm] + glycolate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 11283302; 11785976 [H]