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The map label for this gene is wecC [H]

Identifier: 50841634

GI number: 50841634

Start: 173823

End: 175121

Strand: Direct

Name: wecC [H]

Synonym: PPA0145

Alternate gene names: 50841634

Gene position: 173823-175121 (Clockwise)

Preceding gene: 50841629

Following gene: 50841635

Centisome position: 6.79

GC content: 61.82

Gene sequence:

>1299_bases
ATGAAAATTGCCGTCGTCGCCATGGGCAAGATCGGGCTTCCCCTGGCCGTGCAGTTCGCCGAGAAGGGTCACGACGTCAT
CGGCGTCGACGTGCAGCAGCGCGTCGTGGACGAGATCAACAAGGGCAATGAGCCTTTTCCCGGTGAGGCCTTTCTTGACG
AGAAGTTGAAGAAGCTTGTCCCGGCAGGCAAGCTCACCGCCACCACCGACTACTCCCAGGCCATCCCCGGCGCCGACGCG
ATAGTTCTCGTCGTCCCGCTCTTCGTTAACGACGAGACCTGGGAACCGGACTTCGGCTGGATGGAAAATGCCACCAAGTC
GCTGGCCGAGTACCTCACCCCCGGCACCCTCATCTCCTACGAGACCACCCTGCCAGTCGGCACCACCCGCAATCGCTGGA
AGCCCATGATCGAGGAGATCTCTGGTCTGAGTGAAGGCAAAGACTTCCACCTCGTCTTTTCCCCGGAGCGCGTTCTCACT
GGTCGCGTCTTCGCTGACCTGCGTCGTTACCCGAAACTCGTCGGCGGCTTGAACGACGAGGGCACTGCCAAGGGCATCGA
CTTCTACCAGCAAGTTCTCGACTTCGACGACCGTGACGACCTTCCTCGCGCCAACGGCGTCTGGGACATGGGCACCGCTG
AGGCCGCTGAGATGGCCAAACTTGCCGAGACCACCTACCGTGACGTCAACATTGGCCTGGCCAATCAGTTCGGCAAGTAC
GCTGCGGCCAACGGCATCGACGTCTACAAGGTCATTGACGCCTGCAACTCGCAGCCCTACTCCCACATTCACCGTCCCGG
CATTGCCGTCGGAGGTCATTGCATCCCCGTCTACCCGCGCCTCTACCTATGGACCGACCCGGACGCCACCATCGTCCGCA
CTGCCCGCGAGGCCAACATGACGATGCCCCAGTACTGCGTCGACCAGGCCGCCTCGGTGCTCGGTGATCTGTCCGGTAAA
AAGATTGTCGTCCTTGGCGCTTCCTATCGGGGCAAGGTCAAGGAAACTGCTTTCTCCGGGGTTTTCCCGACTGTTGACAT
CCTCGCCAAGGCTGGCGCTGACGTCAGCGTCCACGACCCGATGTTCACCGACGAGGAGCTCGGCAAGTTCGGCTTTAAGG
CCTACCACATCGGCGATCCGGTTGACGGCGCTCTACTGCAGGCCGACCATCCCGAGTACGCCGAACTGACCCCGGCGGAC
CTGCCGGGTATCAAGGTGCTCGTCGACGGTCGTCACGTCGTCGATCCAGCCGTATGGGGAGACGTCGAGGTCATCGTCGT
TGGTGACGGGGAGGCCTGA

Upstream 100 bases:

>100_bases
TGCGAGCGTCTTTGCGACGGCGTGAGCATAACACGGACTCGTCTAGGTGCCGTACCCGGATAGACTGACGGAGTATTCGC
GAATCCCAAGGAGTTCAGGA

Downstream 100 bases:

>100_bases
GTACTCCGGCAGCTTCCTCGACAAGATCGTCGAGGGGCTGTTGGTGCTGTTGCCTCCGGCAGCCCTCCTGGGGCCCTGGG
CCGGCAGTGGAAGAGTGTTC

Product: putative UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase

Products: UDP-N-acetyl-D-mannosaminuronic-acid; NADH; Proton [C]

Alternate protein names: UDP-ManNAc 6-dehydrogenase [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MKIAVVAMGKIGLPLAVQFAEKGHDVIGVDVQQRVVDEINKGNEPFPGEAFLDEKLKKLVPAGKLTATTDYSQAIPGADA
IVLVVPLFVNDETWEPDFGWMENATKSLAEYLTPGTLISYETTLPVGTTRNRWKPMIEEISGLSEGKDFHLVFSPERVLT
GRVFADLRRYPKLVGGLNDEGTAKGIDFYQQVLDFDDRDDLPRANGVWDMGTAEAAEMAKLAETTYRDVNIGLANQFGKY
AAANGIDVYKVIDACNSQPYSHIHRPGIAVGGHCIPVYPRLYLWTDPDATIVRTAREANMTMPQYCVDQAASVLGDLSGK
KIVVLGASYRGKVKETAFSGVFPTVDILAKAGADVSVHDPMFTDEELGKFGFKAYHIGDPVDGALLQADHPEYAELTPAD
LPGIKVLVDGRHVVDPAVWGDVEVIVVGDGEA

Sequences:

>Translated_432_residues
MKIAVVAMGKIGLPLAVQFAEKGHDVIGVDVQQRVVDEINKGNEPFPGEAFLDEKLKKLVPAGKLTATTDYSQAIPGADA
IVLVVPLFVNDETWEPDFGWMENATKSLAEYLTPGTLISYETTLPVGTTRNRWKPMIEEISGLSEGKDFHLVFSPERVLT
GRVFADLRRYPKLVGGLNDEGTAKGIDFYQQVLDFDDRDDLPRANGVWDMGTAEAAEMAKLAETTYRDVNIGLANQFGKY
AAANGIDVYKVIDACNSQPYSHIHRPGIAVGGHCIPVYPRLYLWTDPDATIVRTAREANMTMPQYCVDQAASVLGDLSGK
KIVVLGASYRGKVKETAFSGVFPTVDILAKAGADVSVHDPMFTDEELGKFGFKAYHIGDPVDGALLQADHPEYAELTPAD
LPGIKVLVDGRHVVDPAVWGDVEVIVVGDGEA
>Mature_432_residues
MKIAVVAMGKIGLPLAVQFAEKGHDVIGVDVQQRVVDEINKGNEPFPGEAFLDEKLKKLVPAGKLTATTDYSQAIPGADA
IVLVVPLFVNDETWEPDFGWMENATKSLAEYLTPGTLISYETTLPVGTTRNRWKPMIEEISGLSEGKDFHLVFSPERVLT
GRVFADLRRYPKLVGGLNDEGTAKGIDFYQQVLDFDDRDDLPRANGVWDMGTAEAAEMAKLAETTYRDVNIGLANQFGKY
AAANGIDVYKVIDACNSQPYSHIHRPGIAVGGHCIPVYPRLYLWTDPDATIVRTAREANMTMPQYCVDQAASVLGDLSGK
KIVVLGASYRGKVKETAFSGVFPTVDILAKAGADVSVHDPMFTDEELGKFGFKAYHIGDPVDGALLQADHPEYAELTPAD
LPGIKVLVDGRHVVDPAVWGDVEVIVVGDGEA

Specific function: Catalyzes the four-electron oxidation of UDP-N-acetyl-D- mannosamine (UDP-ManNAc), reducing NAD(+) and releasing UDP-N- acetylmannosaminuronic acid (UDP-ManNAcA) [H]

COG id: COG0677

COG function: function code M; UDP-N-acetyl-D-mannosaminuronate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UDP-glucose/GDP-mannose dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI48994968, Length=364, Percent_Identity=32.967032967033, Blast_Score=178, Evalue=5e-46,
Organism=Escherichia coli, GI1788340, Length=379, Percent_Identity=20.5804749340369, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR016040
- InterPro:   IPR017476
- InterPro:   IPR014027
- InterPro:   IPR014026
- InterPro:   IPR014028
- InterPro:   IPR001732 [H]

Pfam domain/function: PF00984 UDPG_MGDP_dh; PF03720 UDPG_MGDP_dh_C; PF03721 UDPG_MGDP_dh_N [H]

EC number: 1.1.1.- [C]

Molecular weight: Translated: 46909; Mature: 46909

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAVVAMGKIGLPLAVQFAEKGHDVIGVDVQQRVVDEINKGNEPFPGEAFLDEKLKKLV
CEEEEEEECCCCCCEEEEEHHCCCCEEECCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHC
PAGKLTATTDYSQAIPGADAIVLVVPLFVNDETWEPDFGWMENATKSLAEYLTPGTLISY
CCCCEEEECCHHHHCCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEE
ETTLPVGTTRNRWKPMIEEISGLSEGKDFHLVFSPERVLTGRVFADLRRYPKLVGGLNDE
EEECCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCC
GTAKGIDFYQQVLDFDDRDDLPRANGVWDMGTAEAAEMAKLAETTYRDVNIGLANQFGKY
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEECCHHHHHCCH
AAANGIDVYKVIDACNSQPYSHIHRPGIAVGGHCIPVYPRLYLWTDPDATIVRTAREANM
HHCCCCHHHHHHHHHCCCCCHHHCCCCEEECCEEEEECCEEEEEECCCCCEEEEHHHCCC
TMPQYCVDQAASVLGDLSGKKIVVLGASYRGKVKETAFSGVFPTVDILAKAGADVSVHDP
CCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHCCCCHHHHHHHHCCCCCEECCC
MFTDEELGKFGFKAYHIGDPVDGALLQADHPEYAELTPADLPGIKVLVDGRHVVDPAVWG
CCCHHHHHHHCCEEEECCCCCCCCEEECCCCCCCCCCCCCCCCEEEEECCCEEECCCCCC
DVEVIVVGDGEA
CEEEEEEECCCC
>Mature Secondary Structure
MKIAVVAMGKIGLPLAVQFAEKGHDVIGVDVQQRVVDEINKGNEPFPGEAFLDEKLKKLV
CEEEEEEECCCCCCEEEEEHHCCCCEEECCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHC
PAGKLTATTDYSQAIPGADAIVLVVPLFVNDETWEPDFGWMENATKSLAEYLTPGTLISY
CCCCEEEECCHHHHCCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEE
ETTLPVGTTRNRWKPMIEEISGLSEGKDFHLVFSPERVLTGRVFADLRRYPKLVGGLNDE
EEECCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCC
GTAKGIDFYQQVLDFDDRDDLPRANGVWDMGTAEAAEMAKLAETTYRDVNIGLANQFGKY
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEECCHHHHHCCH
AAANGIDVYKVIDACNSQPYSHIHRPGIAVGGHCIPVYPRLYLWTDPDATIVRTAREANM
HHCCCCHHHHHHHHHCCCCCHHHCCCCEEECCEEEEECCEEEEEECCCCCEEEEHHHCCC
TMPQYCVDQAASVLGDLSGKKIVVLGASYRGKVKETAFSGVFPTVDILAKAGADVSVHDP
CCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHCCCCHHHHHHHHCCCCCEECCC
MFTDEELGKFGFKAYHIGDPVDGALLQADHPEYAELTPADLPGIKVLVDGRHVVDPAVWG
CCCHHHHHHHCCEEEECCCCCCCCEEECCCCCCCCCCCCCCCCEEEEECCCEEECCCCCC
DVEVIVVGDGEA
CEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-N-acetyl-D-mannosamine; NAD [C]

Specific reaction: UDP-N-acetyl-D-mannosamine + NAD = UDP-N-acetyl-D-mannosaminuronic-acid + NADH + Proton [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA