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The map label for this gene is pbpF [H]

Identifier: 50841615

GI number: 50841615

Start: 149657

End: 152002

Strand: Direct

Name: pbpF [H]

Synonym: PPA0126

Alternate gene names: 50841615

Gene position: 149657-152002 (Clockwise)

Preceding gene: 50841614

Following gene: 50841616

Centisome position: 5.85

GC content: 59.8

Gene sequence:

>2346_bases
ATGGCTGACCACAAACCCGCCAGAGCATCGAAGCGCCACGCCCCCGCGACTGGGGGCGGTGCCGGAAGGGCTCGTCGGGC
CGCCGGGAGGCCTAGCCGTCATCCTAGGCTGGGCAAGTTTCTCCGGGGTTTGGTGATCGCAATCTTGTCTTTGGTGCTTA
TCGCCGTCGTTGGGGTCTTTGTCGCCTATGAGCGCACCGAGCTTCCCGACCCTAACCGCGACTTCCAGACGAACACCTCG
TTTATCTACTACCGGGACGGCAGTCGCCTCGGGTCTTTCTCGGTACAGAACCGTCAATCGATCCCTTATGAGACGATGCC
GAAGACTCTGCGTGACGCCGTCGTCTCGGCGGAGAATCGCACTTTCTGGTCCGATCCGGGTATCTCGGTCAAGGGCATGA
CGCGCGCCGCCTGGGCGATCGCTCGCGGTGGTGAGATGCAAGGCGGCTCGACGATCACCCAGCAGTACATCAAGGTGTTG
TACCTGTCCCAGGAACGCACCATGGGTCGCAAATTCAAGGAGCTCCTCCTGGCCGTGAAGACGGGCAAGGAGGTCTCTAA
AAAGGGCATTCTCGCTGGATATCTCAATACCATCTACTTTGGCCGGGGAGCTTATGGTGTTCAGGCTGCCGCTCGGGCTT
TCTTCTACACCGATGCTTCGAAGCTCACCCTGTCTCAGTCGGCGGTGTTGGCTGCGGTGCTTAACAGCCCGTCAAATTTC
GATCCCAGCGGTGGCGTAGGCGCTCGCGAGAGGTTACTCCAGCGATACCGGTACGTCCTCGACGGAATGCTGGAGGCCGG
CAATATCACCCAGGCTCAGCACGACGAGGCTTATCGGCAGCTGCCGAAATTCCCGAAGGTCCCCGACTACAACCGATGGG
CGGGAACCGACGGCTACCTCATGAAGTTGGTTTACGACGAGCTCATCGCCAGAGGGTTCTCCGATCAACAAATTAAGGGC
GGTGGCCTCAAGGTGACGACCACCCTGGATCGTAAGGACCAGCAGGCTGCCGTGGCTGCAGGCCAGAAATATAAGAAAGT
TGCTGGCCGCAACGCTGGTCCTGAGGGAGCCAAGAATCTACATCCTGCGCTTGCCTCAGTCGACGTCAGCTCCGGCGGTG
TCCTGGCCCTTTACGGTGGCGATGACTATATTTCCAATACCCGCGATTGGGCGCTTACTGCGCGCCCGGCGGCATCCACC
TTCAAGACCTACGCGGCCATAGCAGGGATGCGCCATGGATTCAGCTTGCGTTCCCGGTTGGAAGGTAACGCTTTCACACC
TGACGGGGATAGCACCGAGGTTCACAACGAGAACGACCGGAACTACGGCACCGTCAGTTTGCGTCAGGCTATTGCGAAGT
CGATCAACACCGCCTTCGTCGACATGGTCTCACGGATTAAGAATGGTCCGAGGGCTGTTGTTCAGGCCGCTACTGACGCC
GGTTTGTCTCAGGGAACTGGGTGGGATCTGAATAACCGGATCGCTTTGGGCACTGCTGAGGTGAGTCCACTGGCTCAGGC
GGGGGGCTATGCGACGATTGCCAATGACGGCAAAAGGGTTACCCCACACATTGTGGACAAGGTTGTTGACCAGTCAGGCA
AGGTCCTCTGCCAGGCACCCACGCCGTCGAAGCAGACCATTGAGGCTGATATCAGCCATGACGTCAGCTACGCCCTGCAG
TCAGTGGTTGAGGAAGGCACTGGACGGATTGTGGCCGGCTTTGACCATCACGTGGCCGGGAAAACGGGTACTTCGGGAGT
TGGTCACGGGGTGACGTCGGCGTGGTTTGTCGCCTACACCAAGCAGATCTCGACGGCTGTCATGTTTGTGGCGGGGGATA
GCGGCAACGAAAACCTTAACCGCTACGCCCGTGAAGGGGCTACCGGTTTCCACGGTGGTGATTACCCGGCTCGCACCTGG
CTCGACTATATGCAGACGGCGATGAGGGGGATGCCCAACAAGTCCTTCGCTGCTCCAGACTGGGTGAATCTGTCAGGTAA
GCATTATGGCTCCACCAACCGTCCGCAGGTGTCGGTCGAGGATGATTCCGATCGCGACCGGTCGAACCAGAATGATCCAG
AGTCGCTGGGTAGGCCGTCTCCGACGCCGACTCGCACGTCGGCCTCCAGCCCAGAGCCGTCATCGGCGCCTTCTCGGGAG
CAATCCTCTGAGCCCTCAGCGACGAGAACGGCGTCGGCCCATACACACACCTCTAAACCCACACAGACGTCACAGCCTGC
GCACACCTCTAGACCGACTCATACCTCCACCCACACCTCTCGTCCGACCTCGGGCGAGACGACGCACGGTGGGAACCAGC
TGAGTGGTCGGGCACAGAATGGGTAG

Upstream 100 bases:

>100_bases
TGCCCCGACTGTCTGTGGAATCACATCCTCGTCGACTACGTCCTAGGTGACGGTCGACGCCGCCGCCCACCTCGCCATCA
GCCGACCGTGGAGGACATCT

Downstream 100 bases:

>100_bases
CCTGACCACGTGACCATCCCTGATCCGAAAGACGACGCCATGGCAGCTAGGAGGTGTGCTGTCGCACACCCAAATGATGG
ATCCCCGGGTTACACGGCTC

Product: putative penicillin-binding protein

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 781; Mature: 780

Protein sequence:

>781_residues
MADHKPARASKRHAPATGGGAGRARRAAGRPSRHPRLGKFLRGLVIAILSLVLIAVVGVFVAYERTELPDPNRDFQTNTS
FIYYRDGSRLGSFSVQNRQSIPYETMPKTLRDAVVSAENRTFWSDPGISVKGMTRAAWAIARGGEMQGGSTITQQYIKVL
YLSQERTMGRKFKELLLAVKTGKEVSKKGILAGYLNTIYFGRGAYGVQAAARAFFYTDASKLTLSQSAVLAAVLNSPSNF
DPSGGVGARERLLQRYRYVLDGMLEAGNITQAQHDEAYRQLPKFPKVPDYNRWAGTDGYLMKLVYDELIARGFSDQQIKG
GGLKVTTTLDRKDQQAAVAAGQKYKKVAGRNAGPEGAKNLHPALASVDVSSGGVLALYGGDDYISNTRDWALTARPAAST
FKTYAAIAGMRHGFSLRSRLEGNAFTPDGDSTEVHNENDRNYGTVSLRQAIAKSINTAFVDMVSRIKNGPRAVVQAATDA
GLSQGTGWDLNNRIALGTAEVSPLAQAGGYATIANDGKRVTPHIVDKVVDQSGKVLCQAPTPSKQTIEADISHDVSYALQ
SVVEEGTGRIVAGFDHHVAGKTGTSGVGHGVTSAWFVAYTKQISTAVMFVAGDSGNENLNRYAREGATGFHGGDYPARTW
LDYMQTAMRGMPNKSFAAPDWVNLSGKHYGSTNRPQVSVEDDSDRDRSNQNDPESLGRPSPTPTRTSASSPEPSSAPSRE
QSSEPSATRTASAHTHTSKPTQTSQPAHTSRPTHTSTHTSRPTSGETTHGGNQLSGRAQNG

Sequences:

>Translated_781_residues
MADHKPARASKRHAPATGGGAGRARRAAGRPSRHPRLGKFLRGLVIAILSLVLIAVVGVFVAYERTELPDPNRDFQTNTS
FIYYRDGSRLGSFSVQNRQSIPYETMPKTLRDAVVSAENRTFWSDPGISVKGMTRAAWAIARGGEMQGGSTITQQYIKVL
YLSQERTMGRKFKELLLAVKTGKEVSKKGILAGYLNTIYFGRGAYGVQAAARAFFYTDASKLTLSQSAVLAAVLNSPSNF
DPSGGVGARERLLQRYRYVLDGMLEAGNITQAQHDEAYRQLPKFPKVPDYNRWAGTDGYLMKLVYDELIARGFSDQQIKG
GGLKVTTTLDRKDQQAAVAAGQKYKKVAGRNAGPEGAKNLHPALASVDVSSGGVLALYGGDDYISNTRDWALTARPAAST
FKTYAAIAGMRHGFSLRSRLEGNAFTPDGDSTEVHNENDRNYGTVSLRQAIAKSINTAFVDMVSRIKNGPRAVVQAATDA
GLSQGTGWDLNNRIALGTAEVSPLAQAGGYATIANDGKRVTPHIVDKVVDQSGKVLCQAPTPSKQTIEADISHDVSYALQ
SVVEEGTGRIVAGFDHHVAGKTGTSGVGHGVTSAWFVAYTKQISTAVMFVAGDSGNENLNRYAREGATGFHGGDYPARTW
LDYMQTAMRGMPNKSFAAPDWVNLSGKHYGSTNRPQVSVEDDSDRDRSNQNDPESLGRPSPTPTRTSASSPEPSSAPSRE
QSSEPSATRTASAHTHTSKPTQTSQPAHTSRPTHTSTHTSRPTSGETTHGGNQLSGRAQNG
>Mature_780_residues
ADHKPARASKRHAPATGGGAGRARRAAGRPSRHPRLGKFLRGLVIAILSLVLIAVVGVFVAYERTELPDPNRDFQTNTSF
IYYRDGSRLGSFSVQNRQSIPYETMPKTLRDAVVSAENRTFWSDPGISVKGMTRAAWAIARGGEMQGGSTITQQYIKVLY
LSQERTMGRKFKELLLAVKTGKEVSKKGILAGYLNTIYFGRGAYGVQAAARAFFYTDASKLTLSQSAVLAAVLNSPSNFD
PSGGVGARERLLQRYRYVLDGMLEAGNITQAQHDEAYRQLPKFPKVPDYNRWAGTDGYLMKLVYDELIARGFSDQQIKGG
GLKVTTTLDRKDQQAAVAAGQKYKKVAGRNAGPEGAKNLHPALASVDVSSGGVLALYGGDDYISNTRDWALTARPAASTF
KTYAAIAGMRHGFSLRSRLEGNAFTPDGDSTEVHNENDRNYGTVSLRQAIAKSINTAFVDMVSRIKNGPRAVVQAATDAG
LSQGTGWDLNNRIALGTAEVSPLAQAGGYATIANDGKRVTPHIVDKVVDQSGKVLCQAPTPSKQTIEADISHDVSYALQS
VVEEGTGRIVAGFDHHVAGKTGTSGVGHGVTSAWFVAYTKQISTAVMFVAGDSGNENLNRYAREGATGFHGGDYPARTWL
DYMQTAMRGMPNKSFAAPDWVNLSGKHYGSTNRPQVSVEDDSDRDRSNQNDPESLGRPSPTPTRTSASSPEPSSAPSREQ
SSEPSATRTASAHTHTSKPTQTSQPAHTSRPTHTSTHTSRPTSGETTHGGNQLSGRAQNG

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=313, Percent_Identity=32.9073482428115, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI1786343, Length=589, Percent_Identity=26.4855687606112, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1788867, Length=455, Percent_Identity=23.956043956044, Blast_Score=90, Evalue=5e-19,
Organism=Escherichia coli, GI1789601, Length=154, Percent_Identity=40.9090909090909, Blast_Score=87, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.- [C]

Molecular weight: Translated: 83893; Mature: 83762

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADHKPARASKRHAPATGGGAGRARRAAGRPSRHPRLGKFLRGLVIAILSLVLIAVVGVF
CCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VAYERTELPDPNRDFQTNTSFIYYRDGSRLGSFSVQNRQSIPYETMPKTLRDAVVSAENR
HHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
TFWSDPGISVKGMTRAAWAIARGGEMQGGSTITQQYIKVLYLSQERTMGRKFKELLLAVK
EEECCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
TGKEVSKKGILAGYLNTIYFGRGAYGVQAAARAFFYTDASKLTLSQSAVLAAVLNSPSNF
CCHHHHHCCHHHHHHHHHEECCCCHHHHHHHHHHEEECCCHHHHHHHHHHHHHHCCCCCC
DPSGGVGARERLLQRYRYVLDGMLEAGNITQAQHDEAYRQLPKFPKVPDYNRWAGTDGYL
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHH
MKLVYDELIARGFSDQQIKGGGLKVTTTLDRKDQQAAVAAGQKYKKVAGRNAGPEGAKNL
HHHHHHHHHHCCCCCCEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHH
HPALASVDVSSGGVLALYGGDDYISNTRDWALTARPAASTFKTYAAIAGMRHGFSLRSRL
CHHHHEEEECCCCEEEEECCCHHHCCCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHHHC
EGNAFTPDGDSTEVHNENDRNYGTVSLRQAIAKSINTAFVDMVSRIKNGPRAVVQAATDA
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
GLSQGTGWDLNNRIALGTAEVSPLAQAGGYATIANDGKRVTPHIVDKVVDQSGKVLCQAP
CCCCCCCCCCCCEEEEECCCCCHHHHCCCEEEECCCCCCCCHHHHHHHHCCCCCEEEECC
TPSKQTIEADISHDVSYALQSVVEEGTGRIVAGFDHHVAGKTGTSGVGHGVTSAWFVAYT
CCCCCEEEHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHH
KQISTAVMFVAGDSGNENLNRYAREGATGFHGGDYPARTWLDYMQTAMRGMPNKSFAAPD
HHHCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
WVNLSGKHYGSTNRPQVSVEDDSDRDRSNQNDPESLGRPSPTPTRTSASSPEPSSAPSRE
CCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCC
QSSEPSATRTASAHTHTSKPTQTSQPAHTSRPTHTSTHTSRPTSGETTHGGNQLSGRAQN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
G
C
>Mature Secondary Structure 
ADHKPARASKRHAPATGGGAGRARRAAGRPSRHPRLGKFLRGLVIAILSLVLIAVVGVF
CCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VAYERTELPDPNRDFQTNTSFIYYRDGSRLGSFSVQNRQSIPYETMPKTLRDAVVSAENR
HHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
TFWSDPGISVKGMTRAAWAIARGGEMQGGSTITQQYIKVLYLSQERTMGRKFKELLLAVK
EEECCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
TGKEVSKKGILAGYLNTIYFGRGAYGVQAAARAFFYTDASKLTLSQSAVLAAVLNSPSNF
CCHHHHHCCHHHHHHHHHEECCCCHHHHHHHHHHEEECCCHHHHHHHHHHHHHHCCCCCC
DPSGGVGARERLLQRYRYVLDGMLEAGNITQAQHDEAYRQLPKFPKVPDYNRWAGTDGYL
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHH
MKLVYDELIARGFSDQQIKGGGLKVTTTLDRKDQQAAVAAGQKYKKVAGRNAGPEGAKNL
HHHHHHHHHHCCCCCCEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHH
HPALASVDVSSGGVLALYGGDDYISNTRDWALTARPAASTFKTYAAIAGMRHGFSLRSRL
CHHHHEEEECCCCEEEEECCCHHHCCCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHHHC
EGNAFTPDGDSTEVHNENDRNYGTVSLRQAIAKSINTAFVDMVSRIKNGPRAVVQAATDA
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
GLSQGTGWDLNNRIALGTAEVSPLAQAGGYATIANDGKRVTPHIVDKVVDQSGKVLCQAP
CCCCCCCCCCCCEEEEECCCCCHHHHCCCEEEECCCCCCCCHHHHHHHHCCCCCEEEECC
TPSKQTIEADISHDVSYALQSVVEEGTGRIVAGFDHHVAGKTGTSGVGHGVTSAWFVAYT
CCCCCEEEHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHH
KQISTAVMFVAGDSGNENLNRYAREGATGFHGGDYPARTWLDYMQTAMRGMPNKSFAAPD
HHHCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
WVNLSGKHYGSTNRPQVSVEDDSDRDRSNQNDPESLGRPSPTPTRTSASSPEPSSAPSRE
CCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCC
QSSEPSATRTASAHTHTSKPTQTSQPAHTSRPTHTSTHTSRPTSGETTHGGNQLSGRAQN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]