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The map label for this gene is rbsB [H]

Identifier: 50841511

GI number: 50841511

Start: 20350

End: 21291

Strand: Direct

Name: rbsB [H]

Synonym: PPA0017

Alternate gene names: 50841511

Gene position: 20350-21291 (Clockwise)

Preceding gene: 50841510

Following gene: 50841512

Centisome position: 0.79

GC content: 55.94

Gene sequence:

>942_bases
ATGAAGTTCAAGTCTACTAAGGTGCTCGCGACGGCAGTCGCCGTGACGATAATAGCGACTGGTGTGGCCGGTTGTAACCG
TGACGGTGGAGCTGGTTCATCTGCATCGGGCACGGTGGTGTTTGCCGTCTCCACGCAAACTAACCCGTTCTTCGTTCAGC
TTGTGGATGGGGCAAAGAAGGAGGCTAAGGCCAAGGGAGTTAACCTTCAGGTGCAGGACGCGTCAGACGACGCCGCCACC
CAGGCCAACCAGCTTGCTAACGCGGTATCTACCTCAGCCAAAGTAGTTATCGTCAATCCGACTGATTCTGACGCCGTTGC
GCCGTCTGTGAAGGCCCTCAATAAGGCGAAAATCCCGGTGATTACGGTCGACCGCAGCTCCAGCAGCGGTGATGTTGCTT
CCTTCATTGCATCTGACAACATTGCTGGTGGTATCGATGCCGCCAAGAAACTCGCTGAGATCATGGGAGACAAGGGTGAA
GTCATCCACCTACAGGGAACCCCGGGTGCCTCGGCTTCCCGTGACCGTGGTAAGGGATTCAGCGAGGAAATCGCCAAACA
TCCAGGCATCAAAGTGGTCGCCAAGCAGACTGCCAATTTTGATCGCTCTAAGGCTCTCGACGTGACGACTAATCTCATGC
AGGCTCACTCCAAAGCCACGGGTATTTTCGCCGAAAACGACGAGATGGCTATTGGTGCTATTTCCGCCCTGGGCTCGAAG
GCTGGCCAAAGCGCCAAGGTTGTAGGTTTCGACGGTACTGCTGACGGTATCAAGGCCGTTAAGGCTGGAACCATGGGAGC
GACGATTGCTCAGCAGCCTGCCTTCCTGGGCAAGACCGCGGTGGACCAGGCGGCCGACATATTGGCTGGCAAGCAGGTTT
CTAAGACCACCCCAATCAAGGTTGTGCTGGTGACCAAGGACAACGCGAAGGACTACGAATGA

Upstream 100 bases:

>100_bases
TATCGCGCTGGCTGTCGGATTCGATGTCTTCAGGTCTAAGAAAGAATCCTGAATAAGCGCTCATGCACGAACTATTGTGT
AACAAGAGAAAGGTAGAACA

Downstream 100 bases:

>100_bases
GCGGCGCGACAGCGAAAGTCACCGTCGTCGGATCGATCAATGCCGACATGAGGGTCGGCGTGGAGAGATTTCCGGGCCCA
GGGGAGACCCTGGCCGGAGG

Product: periplasmic sugar-binding protein

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MKFKSTKVLATAVAVTIIATGVAGCNRDGGAGSSASGTVVFAVSTQTNPFFVQLVDGAKKEAKAKGVNLQVQDASDDAAT
QANQLANAVSTSAKVVIVNPTDSDAVAPSVKALNKAKIPVITVDRSSSSGDVASFIASDNIAGGIDAAKKLAEIMGDKGE
VIHLQGTPGASASRDRGKGFSEEIAKHPGIKVVAKQTANFDRSKALDVTTNLMQAHSKATGIFAENDEMAIGAISALGSK
AGQSAKVVGFDGTADGIKAVKAGTMGATIAQQPAFLGKTAVDQAADILAGKQVSKTTPIKVVLVTKDNAKDYE

Sequences:

>Translated_313_residues
MKFKSTKVLATAVAVTIIATGVAGCNRDGGAGSSASGTVVFAVSTQTNPFFVQLVDGAKKEAKAKGVNLQVQDASDDAAT
QANQLANAVSTSAKVVIVNPTDSDAVAPSVKALNKAKIPVITVDRSSSSGDVASFIASDNIAGGIDAAKKLAEIMGDKGE
VIHLQGTPGASASRDRGKGFSEEIAKHPGIKVVAKQTANFDRSKALDVTTNLMQAHSKATGIFAENDEMAIGAISALGSK
AGQSAKVVGFDGTADGIKAVKAGTMGATIAQQPAFLGKTAVDQAADILAGKQVSKTTPIKVVLVTKDNAKDYE
>Mature_313_residues
MKFKSTKVLATAVAVTIIATGVAGCNRDGGAGSSASGTVVFAVSTQTNPFFVQLVDGAKKEAKAKGVNLQVQDASDDAAT
QANQLANAVSTSAKVVIVNPTDSDAVAPSVKALNKAKIPVITVDRSSSSGDVASFIASDNIAGGIDAAKKLAEIMGDKGE
VIHLQGTPGASASRDRGKGFSEEIAKHPGIKVVAKQTANFDRSKALDVTTNLMQAHSKATGIFAENDEMAIGAISALGSK
AGQSAKVVGFDGTADGIKAVKAGTMGATIAQQPAFLGKTAVDQAADILAGKQVSKTTPIKVVLVTKDNAKDYE

Specific function: Involved in the high-affinity D-ribose membrane transport system and also serves as the primary chemoreceptor for chemotaxis [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=306, Percent_Identity=43.1372549019608, Blast_Score=232, Evalue=3e-62,
Organism=Escherichia coli, GI1790526, Length=257, Percent_Identity=33.0739299610895, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1790674, Length=256, Percent_Identity=31.640625, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789990, Length=240, Percent_Identity=28.75, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 31672; Mature: 31672

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFKSTKVLATAVAVTIIATGVAGCNRDGGAGSSASGTVVFAVSTQTNPFFVQLVDGAKK
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCH
EAKAKGVNLQVQDASDDAATQANQLANAVSTSAKVVIVNPTDSDAVAPSVKALNKAKIPV
HHHHCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHCCCCCCE
ITVDRSSSSGDVASFIASDNIAGGIDAAKKLAEIMGDKGEVIHLQGTPGASASRDRGKGF
EEEECCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCH
SEEIAKHPGIKVVAKQTANFDRSKALDVTTNLMQAHSKATGIFAENDEMAIGAISALGSK
HHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCEEECCCCEEEHHHHHHHHC
AGQSAKVVGFDGTADGIKAVKAGTMGATIAQQPAFLGKTAVDQAADILAGKQVSKTTPIK
CCCCEEEEEECCCCHHHHHHHCCCCCCHHHHCCCHHCHHHHHHHHHHHCCCCCCCCCCEE
VVLVTKDNAKDYE
EEEEECCCCCCCC
>Mature Secondary Structure
MKFKSTKVLATAVAVTIIATGVAGCNRDGGAGSSASGTVVFAVSTQTNPFFVQLVDGAKK
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCH
EAKAKGVNLQVQDASDDAATQANQLANAVSTSAKVVIVNPTDSDAVAPSVKALNKAKIPV
HHHHCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHCCCCCCE
ITVDRSSSSGDVASFIASDNIAGGIDAAKKLAEIMGDKGEVIHLQGTPGASASRDRGKGF
EEEECCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCH
SEEIAKHPGIKVVAKQTANFDRSKALDVTTNLMQAHSKATGIFAENDEMAIGAISALGSK
HHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCEEECCCCEEEHHHHHHHHC
AGQSAKVVGFDGTADGIKAVKAGTMGATIAQQPAFLGKTAVDQAADILAGKQVSKTTPIK
CCCCEEEEEECCCCHHHHHHHCCCCCCHHHHCCCHHCHHHHHHHHHHHCCCCCCCCCCEE
VVLVTKDNAKDYE
EEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6313683; 7686882; 9278503; 3011793; 3011794; 8899705; 9298646; 1583688; 7982928; 9641984 [H]