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The map label for this gene is ganB

Identifier: 50812295

GI number: 50812295

Start: 3501651

End: 3502940

Strand: Reverse

Name: ganB

Synonym: BSU34120

Alternate gene names: 50812295

Gene position: 3502940-3501651 (Counterclockwise)

Preceding gene: 16080466

Following gene: 16080462

Centisome position: 83.09

GC content: 46.74

Gene sequence:

>1290_bases
ATGAAAAGCAAAGTGAAAATGTTCTTTGCGGCTGCCATCGTGTGGAGTGCATGTAGTTCAACAGGATATGCCGCTGCCAT
TGAGAAGGAGAAGCACGTGTCAGAGCTTCGGGCAGAGGATCTTTTTGTTAAAAAAGTAGAGGGGATGAACAAGGATTTTA
TCAAAGGGGCAGATGTATCCAGCGTTATTGCTTTGGAAAACAGCGGTGTCACCTTTTACAATACAAACGGAAAACGCCAG
GATATCTTTACAACTTTAAAACAGGCTGGGGTCAACTATGTTCGCGTCCGCATCTGGAATCACCCGTATGATTCAAATGG
CAACGGGTATGGCGGGGGAAACAATGATGTTCAAAAAGCCATCGAAATCGGAAAAAGAGCGACAGCGAACGGAATGAAGG
TGCTGGCCGACTTTCACTACTCTGATTTCTGGGCCGATCCAGCGAAACAAAAGGTGCCCAAAGCCTGGGCGAATCTCAGC
TTTGAAGCAAAAAAAGCAAAGCTCTATGAGTATACGAAACAAAGCCTGCAAAAGATGATCAAGGAAGGCGTTGACATCGG
CATGGTTCAGGTCGGAAATGAAACAACAGGAGGATTTGCCGGTGAGACTGATTGGACGAAGATGTGCCAATTATTTAATG
AAGGAAGCCGAGCGGTCAGGGAGACAAATTCAAATATTTTGGTCGCCCTGCATTTTACCAATCCTGAAACGGCTGGAAGG
TATTCATTTATTGCAGAAACACTCAGCAAAAACAAAGTGGATTATGATGTGTTTGCTAGCTCCTATTATCCTTTCTGGCA
TGGCACATTACAAAATTTGACCTCCGTGCTGAAGGCTGTTGCCAATACGTACGGCAAAAAAGTCATGGTGGCGGAGACAT
CGTACACCTATACCGCTGAGGATGGCGATGGGCATGGAAATACAGCACCAAAAAGCGGGCAGACGTTGCCATATCCAATT
TCTGTTCAAGGCCAGGCAACTGCAGTAAGAGATGTAATGGAGGCAGTGGCGAATACGGGCAAAGCAGGACTTGGTGTTTT
CTACTGGGAGCCGGCGTGGATTCCAGTCGGGCCGAAGACACAGATAGAGAAAAACAAAGTGTTATGGGAAACATACGGGT
CAGGGTGGGCGTCCAGCTATGCTGCTGAATACGACCCTGAAGACGCCGGGAAGTGGTATGGGGGAAGTGCTGTAGATAAT
CAAGCTTTATTTGATTTTAATGGACACCCGCTGCCTTCCTTGCAGGTGTTTCAATATGCGGAGTCAGGACATATTCCAAA
GAAACGCTGA

Upstream 100 bases:

>100_bases
ATTGTCGTAAACACACATTAGGCTGATGCTCCGCTCGATATGGGCGGATTCTTTTTTCTATAGAATGAAAACGCTTGCTA
AGTCTTGGGGGGATGAAATC

Downstream 100 bases:

>100_bases
AACAAAAAAATCCATGAGATGTAACAAATCATTGTTATGTCTCATGGATCATTTTATTTTACATCAACTAATATAAAACA
TTCGTCATCACTCTTATGAT

Product: secreted arabinogalactan oligomer endo-hydrolase

Products: NA

Alternate protein names: Endo-1,4-beta-galactanase; Galactanase

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MKSKVKMFFAAAIVWSACSSTGYAAAIEKEKHVSELRAEDLFVKKVEGMNKDFIKGADVSSVIALENSGVTFYNTNGKRQ
DIFTTLKQAGVNYVRVRIWNHPYDSNGNGYGGGNNDVQKAIEIGKRATANGMKVLADFHYSDFWADPAKQKVPKAWANLS
FEAKKAKLYEYTKQSLQKMIKEGVDIGMVQVGNETTGGFAGETDWTKMCQLFNEGSRAVRETNSNILVALHFTNPETAGR
YSFIAETLSKNKVDYDVFASSYYPFWHGTLQNLTSVLKAVANTYGKKVMVAETSYTYTAEDGDGHGNTAPKSGQTLPYPI
SVQGQATAVRDVMEAVANTGKAGLGVFYWEPAWIPVGPKTQIEKNKVLWETYGSGWASSYAAEYDPEDAGKWYGGSAVDN
QALFDFNGHPLPSLQVFQYAESGHIPKKR

Sequences:

>Translated_429_residues
MKSKVKMFFAAAIVWSACSSTGYAAAIEKEKHVSELRAEDLFVKKVEGMNKDFIKGADVSSVIALENSGVTFYNTNGKRQ
DIFTTLKQAGVNYVRVRIWNHPYDSNGNGYGGGNNDVQKAIEIGKRATANGMKVLADFHYSDFWADPAKQKVPKAWANLS
FEAKKAKLYEYTKQSLQKMIKEGVDIGMVQVGNETTGGFAGETDWTKMCQLFNEGSRAVRETNSNILVALHFTNPETAGR
YSFIAETLSKNKVDYDVFASSYYPFWHGTLQNLTSVLKAVANTYGKKVMVAETSYTYTAEDGDGHGNTAPKSGQTLPYPI
SVQGQATAVRDVMEAVANTGKAGLGVFYWEPAWIPVGPKTQIEKNKVLWETYGSGWASSYAAEYDPEDAGKWYGGSAVDN
QALFDFNGHPLPSLQVFQYAESGHIPKKR
>Mature_429_residues
MKSKVKMFFAAAIVWSACSSTGYAAAIEKEKHVSELRAEDLFVKKVEGMNKDFIKGADVSSVIALENSGVTFYNTNGKRQ
DIFTTLKQAGVNYVRVRIWNHPYDSNGNGYGGGNNDVQKAIEIGKRATANGMKVLADFHYSDFWADPAKQKVPKAWANLS
FEAKKAKLYEYTKQSLQKMIKEGVDIGMVQVGNETTGGFAGETDWTKMCQLFNEGSRAVRETNSNILVALHFTNPETAGR
YSFIAETLSKNKVDYDVFASSYYPFWHGTLQNLTSVLKAVANTYGKKVMVAETSYTYTAEDGDGHGNTAPKSGQTLPYPI
SVQGQATAVRDVMEAVANTGKAGLGVFYWEPAWIPVGPKTQIEKNKVLWETYGSGWASSYAAEYDPEDAGKWYGGSAVDN
QALFDFNGHPLPSLQVFQYAESGHIPKKR

Specific function: Hydrolyzes the beta-1,4-galactan linkages of arabinogalactan type I, a pectic substance found in plants such as soybeans

COG id: COG3867

COG function: function code G; Arabinogalactan endo-1,4-beta-galactosidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 53 family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GANA_BACSU (O07013)

Other databases:

- EMBL:   Z94043
- EMBL:   AL009126
- PIR:   F70038
- RefSeq:   NP_391292.2
- ProteinModelPortal:   O07013
- SMR:   O07013
- EnsemblBacteria:   EBBACT00000000641
- GeneID:   937086
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU34120
- NMPDR:   fig|224308.1.peg.3418
- GenoList:   BSU34120
- GeneTree:   EBGT00050000002402
- HOGENOM:   HBG465679
- OMA:   EGKSWGQ
- ProtClustDB:   CLSK764973
- BioCyc:   BSUB:BSU34120-MONOMER
- InterPro:   IPR011683
- InterPro:   IPR017853
- InterPro:   IPR013781
- Gene3D:   G3DSA:3.20.20.80

Pfam domain/function: PF07745 Glyco_hydro_53; SSF51445 Glyco_hydro_cat

EC number: =3.2.1.89

Molecular weight: Translated: 47279; Mature: 47279

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS00013 PROKAR_LIPOPROTEIN

Important sites: ACT_SITE 194-194 ACT_SITE 292-292 BINDING 267-267 BINDING 296-296 BINDING 311-311 BINDING 388-388

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSKVKMFFAAAIVWSACSSTGYAAAIEKEKHVSELRAEDLFVKKVEGMNKDFIKGADVS
CCCHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCC
SVIALENSGVTFYNTNGKRQDIFTTLKQAGVNYVRVRIWNHPYDSNGNGYGGGNNDVQKA
EEEEEECCCEEEEECCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHH
IEIGKRATANGMKVLADFHYSDFWADPAKQKVPKAWANLSFEAKKAKLYEYTKQSLQKMI
HHHCCCCCCCCCEEEEECCCHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
KEGVDIGMVQVGNETTGGFAGETDWTKMCQLFNEGSRAVRETNSNILVALHFTNPETAGR
HCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCH
YSFIAETLSKNKVDYDVFASSYYPFWHGTLQNLTSVLKAVANTYGKKVMVAETSYTYTAE
HHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEEEC
DGDGHGNTAPKSGQTLPYPISVQGQATAVRDVMEAVANTGKAGLGVFYWEPAWIPVGPKT
CCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCEEEEECCEEEECCCCC
QIEKNKVLWETYGSGWASSYAAEYDPEDAGKWYGGSAVDNQALFDFNGHPLPSLQVFQYA
CCCCCCEEEEECCCCHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHH
ESGHIPKKR
HCCCCCCCC
>Mature Secondary Structure
MKSKVKMFFAAAIVWSACSSTGYAAAIEKEKHVSELRAEDLFVKKVEGMNKDFIKGADVS
CCCHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCC
SVIALENSGVTFYNTNGKRQDIFTTLKQAGVNYVRVRIWNHPYDSNGNGYGGGNNDVQKA
EEEEEECCCEEEEECCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHH
IEIGKRATANGMKVLADFHYSDFWADPAKQKVPKAWANLSFEAKKAKLYEYTKQSLQKMI
HHHCCCCCCCCCEEEEECCCHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
KEGVDIGMVQVGNETTGGFAGETDWTKMCQLFNEGSRAVRETNSNILVALHFTNPETAGR
HCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCH
YSFIAETLSKNKVDYDVFASSYYPFWHGTLQNLTSVLKAVANTYGKKVMVAETSYTYTAE
HHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEEEC
DGDGHGNTAPKSGQTLPYPISVQGQATAVRDVMEAVANTGKAGLGVFYWEPAWIPVGPKT
CCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCEEEEECCEEEECCCCC
QIEKNKVLWETYGSGWASSYAAEYDPEDAGKWYGGSAVDNQALFDFNGHPLPSLQVFQYA
CCCCCCEEEEECCCCHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHH
ESGHIPKKR
HCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377