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The map label for this gene is yitB

Identifier: 50812215

GI number: 50812215

Start: 1171755

End: 1172465

Strand: Reverse

Name: yitB

Synonym: BSU10930

Alternate gene names: 50812215

Gene position: 1172465-1171755 (Counterclockwise)

Preceding gene: 16078160

Following gene: 50812214

Centisome position: 27.81

GC content: 48.24

Gene sequence:

>711_bases
TTGAACGAAACCATCACATATGACACTTGGAACGACATGCTGTCCAAACAGATAACGGATCAATTGATCGATGAGCTCGA
TGTGTTGAAATGGGCTTACCGAACGTACGGAGAAAAGATTGTGTACGCCTGCAGCTTTGGCGCAGAAGGAATGGTGCTTC
TCGACTTAATATCAAAAATCAACAAAAACGCACACATCATCTTTTTGGATACCGGGCTTCATTTTCAGGAAACATATGAG
CTGATCGAGACCGTCAAAGAACGATATCCGGGATTTGCAATCCAGATGCTCGAACCCGAGCTGAGCTTAACAGAACAGGG
GACGAAGTACGGCGGAGAGCTGTGGAAGCATAACCCGAACCTCTGCTGCCAATTGCGGAAAATCGAACCGCTCAAAAAGC
ACCTGTCCGGCATGACAGCTTGGATTTCAGGACTGCGCCGGGATCAGTCACCAACGAGAAAGCATATTCAATACGTAAAC
CTGGACCAGAAGTTTGAGCTCATCAAAATTTGCCCGCTTATCCACTGGACATGGGATGACGTTTGGACGTACATCCGCCT
GCACAATTTGCCTTATAACAAACTGCACGATCAACATTATCCGAGCATAGGCTGTGAAATGTGTACACTGCCTTCCCCGG
ACCCGAACGATGAGAGGGCTGGAAGATGGGCCGGACGGGAGAAAACAGAATGCGGCCTGCATCAAGAATAG

Upstream 100 bases:

>100_bases
GGCGCGGCACCTAAAACCTGAACCTTTATGCAAAAAATGAATAGTCTGCCTATAACTCGATCAATAGATAAAAAAAGCAG
ATGCAAACGGGGGGAAAACA

Downstream 100 bases:

>100_bases
CGGATGGCAGGTTCTTGATGTGAGAATCGAAATGATGTTAAGGCGCTGATGTCAAACTTTCATTAAGTGCAGAAAAGAAA
ACAAGGAAACGTATAAATGG

Product: phospho-adenylylsulfate sulfotransferase

Products: NA

Alternate protein names: 3'-phosphoadenylylsulfate reductase; PAPS reductase, thioredoxin dependent; PAPS sulfotransferase; PAdoPS reductase

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MNETITYDTWNDMLSKQITDQLIDELDVLKWAYRTYGEKIVYACSFGAEGMVLLDLISKINKNAHIIFLDTGLHFQETYE
LIETVKERYPGFAIQMLEPELSLTEQGTKYGGELWKHNPNLCCQLRKIEPLKKHLSGMTAWISGLRRDQSPTRKHIQYVN
LDQKFELIKICPLIHWTWDDVWTYIRLHNLPYNKLHDQHYPSIGCEMCTLPSPDPNDERAGRWAGREKTECGLHQE

Sequences:

>Translated_236_residues
MNETITYDTWNDMLSKQITDQLIDELDVLKWAYRTYGEKIVYACSFGAEGMVLLDLISKINKNAHIIFLDTGLHFQETYE
LIETVKERYPGFAIQMLEPELSLTEQGTKYGGELWKHNPNLCCQLRKIEPLKKHLSGMTAWISGLRRDQSPTRKHIQYVN
LDQKFELIKICPLIHWTWDDVWTYIRLHNLPYNKLHDQHYPSIGCEMCTLPSPDPNDERAGRWAGREKTECGLHQE
>Mature_236_residues
MNETITYDTWNDMLSKQITDQLIDELDVLKWAYRTYGEKIVYACSFGAEGMVLLDLISKINKNAHIIFLDTGLHFQETYE
LIETVKERYPGFAIQMLEPELSLTEQGTKYGGELWKHNPNLCCQLRKIEPLKKHLSGMTAWISGLRRDQSPTRKHIQYVN
LDQKFELIKICPLIHWTWDDVWTYIRLHNLPYNKLHDQHYPSIGCEMCTLPSPDPNDERAGRWAGREKTECGLHQE

Specific function: Reduction of activated sulfate into sulfite

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysH subfamily

Homologues:

Organism=Escherichia coli, GI1789121, Length=231, Percent_Identity=28.1385281385281, Blast_Score=91, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6325425, Length=238, Percent_Identity=32.7731092436975, Blast_Score=131, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSH2_BACSU (O06737)

Other databases:

- EMBL:   Y09476
- EMBL:   AL009126
- PIR:   C69839
- RefSeq:   NP_388974.2
- ProteinModelPortal:   O06737
- SMR:   O06737
- EnsemblBacteria:   EBBACT00000001541
- GeneID:   939787
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU10930
- NMPDR:   fig|224308.1.peg.1093
- GenoList:   BSU10930
- GeneTree:   EBGT00050000002870
- HOGENOM:   HBG758022
- OMA:   PLANWTF
- PhylomeDB:   O06737
- ProtClustDB:   CLSK737559
- BioCyc:   BSUB:BSU10930-MONOMER
- BRENDA:   1.8.4.8
- GO:   GO:0005737
- HAMAP:   MF_00063
- InterPro:   IPR011798
- InterPro:   IPR004511
- InterPro:   IPR002500
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR02055
- TIGRFAMs:   TIGR00434

Pfam domain/function: PF01507 PAPS_reduct

EC number: =1.8.4.8

Molecular weight: Translated: 27671; Mature: 27671

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNETITYDTWNDMLSKQITDQLIDELDVLKWAYRTYGEKIVYACSFGAEGMVLLDLISKI
CCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHH
NKNAHIIFLDTGLHFQETYELIETVKERYPGFAIQMLEPELSLTEQGTKYGGELWKHNPN
CCCCEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHCCCHHCCHHHHCCCC
LCCQLRKIEPLKKHLSGMTAWISGLRRDQSPTRKHIQYVNLDQKFELIKICPLIHWTWDD
CEEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHEECCCCCHHHHHHHHHHHEECHHH
VWTYIRLHNLPYNKLHDQHYPSIGCEMCTLPSPDPNDERAGRWAGREKTECGLHQE
HHHHHHHCCCCCHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNETITYDTWNDMLSKQITDQLIDELDVLKWAYRTYGEKIVYACSFGAEGMVLLDLISKI
CCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHH
NKNAHIIFLDTGLHFQETYELIETVKERYPGFAIQMLEPELSLTEQGTKYGGELWKHNPN
CCCCEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHCCCHHCCHHHHCCCC
LCCQLRKIEPLKKHLSGMTAWISGLRRDQSPTRKHIQYVNLDQKFELIKICPLIHWTWDD
CEEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHEECCCCCHHHHHHHHHHHEECHHH
VWTYIRLHNLPYNKLHDQHYPSIGCEMCTLPSPDPNDERAGRWAGREKTECGLHQE
HHHHHHHCCCCCHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377