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The map label for this gene is mnmG [H]

Identifier: 50365486

GI number: 50365486

Start: 779747

End: 781627

Strand: Reverse

Name: mnmG [H]

Synonym: Mfl671

Alternate gene names: 50365486

Gene position: 781627-779747 (Counterclockwise)

Preceding gene: 50365487

Following gene: 50365485

Centisome position: 98.54

GC content: 31.21

Gene sequence:

>1881_bases
ATGAATAAACATTTTGATGTTATTGTTATTGGTGGTGGTCATGCAGGTGTTGAAGCTGCCTTGGCATCTGCAAGATTAAA
CAAAAAAACAGCATTAATTAATCTTTATAAAGATAGAATTGCCGCAATGCCTTGCAACCCAAGTGTTGGTGGACCTGCAA
AAGGTATAGTTGTAAGAGAAATTGACGCATTAGGTGGCGAAATGGCAAAAGCTGCTGATAAAACAGCTTTACAAACAAAA
CTACTCAATTCTTCTAGAGGGCCTGGTGTTTGAGCACTAAGAGTCCAATCAGATAAAGAAGAATATTCAAAATATATGCA
AAAAATTGTTGAAGAACAAATAAATTTACAATTAATAGAGTCTATTGCAACAGATCTTATTATTGAAAATAATACAGTAA
CGGGTGTTTTATTAAAAGATGGAGAAATTTTAAAATCAAAATGTGTTGTTTTAACAACGGGAACATATTTAAAATCAGAG
ATCTTAACTGGACATGAAAAATTTTCTTCAGGCCCAAACGAAGAACCGACATCTAATGGAATAAGTGAATCTTTAAAAAA
ATCTGGAGTTGAACTTTTTAGATTTAAAACAGGAACTCCTCCAAGAATATACAAAAACTCTGTTGATCTTTCTAAAGCTA
TTAAAGAACCAGGGACTGACGCAAAATTAGCTTTCAGTTTTTCTACAAATGAATACACACCAATTGAAAAGCAAGAACTT
TGCTACTTAATTCATTCAAGTTCTGAAACAAAAAAAATAATCGAAGATAATTTGACAAAATCAGCAATGTATTCAGGTAA
AGTAGAATCTATAGGTCCTAGATACTGTCCAAGTTTTGAAGATAAAATTGTAAGATTTTCATCAAAAGAAACTCATCAAA
TTTTTATTGAGCCAGAATCAATTTCTGGTGATGCTTGATATATTCAGGGATTTTCAACTTCAATGCCAACAGATGTTCAA
TTGTTAATGATAAGATCATTGCCAGGTTTTGAAAAAGCTGAAGTAAAAACTTGGGCATATGCCATTGAGTACGATTGTGT
AAATCCTATGCAATTGAATCCCTCATTAGAAATTAAGAATATTAATAATTTATTTACAGCAGGTCAAATAAATGGAACTA
GTGGTTATGAAGAGGCAGCTGGCCAAGGATTAATTGCTGGTATTAACGCATCTAGAAAAATTGATGGATTAGATCCTTTG
ATTTTAAGAAGAGATGAAGCATATATAGGTGTCATGATAGATGATTTAATTAACAAAGGCGTATGGGAACCTTATAGACT
TTTAACAAGTAGGGCAGAACACAGATTATTGTTAAGAAATGATAACGCTGAACATAGGCTAAAAGAAAAAGGATATGAGA
TAGGCCTAATTAAACAAGCAGAATATAATGAATACTTGAAATACACAGAAGAAATTAATAACGCAATAAAAGAGCTTGAA
GAAATCAGATTTACTCCTAAGTCTGAACTAGCAATAAAATTAAAAGAATTAGGTCAAGCAGACTTAAGTCATGGATATAG
TGGTATTGAAATTTTAAAAATACCAACTGTTGATATAGAGGTTATGATTCCGTACATTAGTTCTTTACAAAACCTAAAAA
ATAATCAACTTCAGTCTATTGTAATAGAAACTAGATTTGCCGGTTATGTAAAAACAGAAAGAAAAACTGTTGAAAGACTT
GTTAAGCTTGAAAAGAAAAAAATACCTTCAGATATTGATTATGATAAGGTTGAAAATTTAGCAACAGAAGCTAGACAAAA
GTTAAAAAAAGTTAAACCATTAAACATAGGACAAGCTTCTAGAATTACTGGAGTTAACCCTGCAGACATACAAATGTTGC
TGTTTTATCTTAAAAATAACTATGCAACGGAGCAAAAGTAA

Upstream 100 bases:

>100_bases
TGTTAAGGTATTTTTTACTTTAAGTAATTTATTTATAATTGCTTATAAATTAAAAAAGACTAAAATTAAATAGACATAAA
ATTAGAAAAGGAGAATTGCT

Downstream 100 bases:

>100_bases
CACTAAAAGTTCACTCAGCAAAATAATTTGTTTATAATTATATAAAAAAGAAGGAGGCATAATCATGTCAATCGAAATGA
AAAATAAAAAGCACACAAGA

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 626; Mature: 626

Protein sequence:

>626_residues
MNKHFDVIVIGGGHAGVEAALASARLNKKTALINLYKDRIAAMPCNPSVGGPAKGIVVREIDALGGEMAKAADKTALQTK
LLNSSRGPGVWALRVQSDKEEYSKYMQKIVEEQINLQLIESIATDLIIENNTVTGVLLKDGEILKSKCVVLTTGTYLKSE
ILTGHEKFSSGPNEEPTSNGISESLKKSGVELFRFKTGTPPRIYKNSVDLSKAIKEPGTDAKLAFSFSTNEYTPIEKQEL
CYLIHSSSETKKIIEDNLTKSAMYSGKVESIGPRYCPSFEDKIVRFSSKETHQIFIEPESISGDAWYIQGFSTSMPTDVQ
LLMIRSLPGFEKAEVKTWAYAIEYDCVNPMQLNPSLEIKNINNLFTAGQINGTSGYEEAAGQGLIAGINASRKIDGLDPL
ILRRDEAYIGVMIDDLINKGVWEPYRLLTSRAEHRLLLRNDNAEHRLKEKGYEIGLIKQAEYNEYLKYTEEINNAIKELE
EIRFTPKSELAIKLKELGQADLSHGYSGIEILKIPTVDIEVMIPYISSLQNLKNNQLQSIVIETRFAGYVKTERKTVERL
VKLEKKKIPSDIDYDKVENLATEARQKLKKVKPLNIGQASRITGVNPADIQMLLFYLKNNYATEQK

Sequences:

>Translated_626_residues
MNKHFDVIVIGGGHAGVEAALASARLNKKTALINLYKDRIAAMPCNPSVGGPAKGIVVREIDALGGEMAKAADKTALQTK
LLNSSRGPGV*ALRVQSDKEEYSKYMQKIVEEQINLQLIESIATDLIIENNTVTGVLLKDGEILKSKCVVLTTGTYLKSE
ILTGHEKFSSGPNEEPTSNGISESLKKSGVELFRFKTGTPPRIYKNSVDLSKAIKEPGTDAKLAFSFSTNEYTPIEKQEL
CYLIHSSSETKKIIEDNLTKSAMYSGKVESIGPRYCPSFEDKIVRFSSKETHQIFIEPESISGDA*YIQGFSTSMPTDVQ
LLMIRSLPGFEKAEVKTWAYAIEYDCVNPMQLNPSLEIKNINNLFTAGQINGTSGYEEAAGQGLIAGINASRKIDGLDPL
ILRRDEAYIGVMIDDLINKGVWEPYRLLTSRAEHRLLLRNDNAEHRLKEKGYEIGLIKQAEYNEYLKYTEEINNAIKELE
EIRFTPKSELAIKLKELGQADLSHGYSGIEILKIPTVDIEVMIPYISSLQNLKNNQLQSIVIETRFAGYVKTERKTVERL
VKLEKKKIPSDIDYDKVENLATEARQKLKKVKPLNIGQASRITGVNPADIQMLLFYLKNNYATEQK
>Mature_626_residues
MNKHFDVIVIGGGHAGVEAALASARLNKKTALINLYKDRIAAMPCNPSVGGPAKGIVVREIDALGGEMAKAADKTALQTK
LLNSSRGPGV*ALRVQSDKEEYSKYMQKIVEEQINLQLIESIATDLIIENNTVTGVLLKDGEILKSKCVVLTTGTYLKSE
ILTGHEKFSSGPNEEPTSNGISESLKKSGVELFRFKTGTPPRIYKNSVDLSKAIKEPGTDAKLAFSFSTNEYTPIEKQEL
CYLIHSSSETKKIIEDNLTKSAMYSGKVESIGPRYCPSFEDKIVRFSSKETHQIFIEPESISGDA*YIQGFSTSMPTDVQ
LLMIRSLPGFEKAEVKTWAYAIEYDCVNPMQLNPSLEIKNINNLFTAGQINGTSGYEEAAGQGLIAGINASRKIDGLDPL
ILRRDEAYIGVMIDDLINKGVWEPYRLLTSRAEHRLLLRNDNAEHRLKEKGYEIGLIKQAEYNEYLKYTEEINNAIKELE
EIRFTPKSELAIKLKELGQADLSHGYSGIEILKIPTVDIEVMIPYISSLQNLKNNQLQSIVIETRFAGYVKTERKTVERL
VKLEKKKIPSDIDYDKVENLATEARQKLKKVKPLNIGQASRITGVNPADIQMLLFYLKNNYATEQK

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=638, Percent_Identity=39.3416927899687, Blast_Score=395, Evalue=1e-110,
Organism=Homo sapiens, GI19882217, Length=663, Percent_Identity=37.8582202111614, Blast_Score=382, Evalue=1e-106,
Organism=Homo sapiens, GI183227703, Length=678, Percent_Identity=37.0206489675516, Blast_Score=376, Evalue=1e-104,
Organism=Escherichia coli, GI2367273, Length=617, Percent_Identity=41.9773095623987, Blast_Score=493, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI17534255, Length=627, Percent_Identity=37.6395534290271, Blast_Score=405, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6321202, Length=623, Percent_Identity=40.2889245585875, Blast_Score=424, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24658174, Length=630, Percent_Identity=40.6349206349206, Blast_Score=421, Evalue=1e-118,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 69508; Mature: 69508

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKHFDVIVIGGGHAGVEAALASARLNKKTALINLYKDRIAAMPCNPSVGGPAKGIVVRE
CCCCEEEEEECCCCHHHHHHHHHHHCCCHHHHEEEHHHHHEECCCCCCCCCCCCCEEEEE
IDALGGEMAKAADKTALQTKLLNSSRGPGVALRVQSDKEEYSKYMQKIVEEQINLQLIES
HHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
IATDLIIENNTVTGVLLKDGEILKSKCVVLTTGTYLKSEILTGHEKFSSGPNEEPTSNGI
HHHHEEEECCEEEEEEEECCHHHHCCEEEEEECCHHHHHHHCCHHHHCCCCCCCCCCCCH
SESLKKSGVELFRFKTGTPPRIYKNSVDLSKAIKEPGTDAKLAFSFSTNEYTPIEKQELC
HHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHCCCCCCEEEEEECCCCCCCCCHHHEE
YLIHSSSETKKIIEDNLTKSAMYSGKVESIGPRYCPSFEDKIVRFSSKETHQIFIEPESI
EEEECCCHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCHHHHHEECCCCCCEEEEEECCCC
SGDAYIQGFSTSMPTDVQLLMIRSLPGFEKAEVKTWAYAIEYDCVNPMQLNPSLEIKNIN
CCCEEEEECCCCCCCCEEEEEEECCCCCCHHHEEEEEEEEEECCCCCEECCCCEEEECCC
NLFTAGQINGTSGYEEAAGQGLIAGINASRKIDGLDPLILRRDEAYIGVMIDDLINKGVW
CEEECCCCCCCCCHHHHCCCCEEEECCCCCCCCCCCCEEEECCCCEEEEEHHHHHCCCCC
EPYRLLTSRAEHRLLLRNDNAEHRLKEKGYEIGLIKQAEYNEYLKYTEEINNAIKELEEI
CHHHHHHHHHHCEEEEECCCHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHH
RFTPKSELAIKLKELGQADLSHGYSGIEILKIPTVDIEVMIPYISSLQNLKNNQLQSIVI
CCCCCHHHHHHHHHHCCHHHHCCCCCEEEEEECCCEEEEHHHHHHHHHHHCCCCHHEEEE
ETRFAGYVKTERKTVERLVKLEKKKIPSDIDYDKVENLATEARQKLKKVKPLNIGQASRI
EHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
TGVNPADIQMLLFYLKNNYATEQK
CCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNKHFDVIVIGGGHAGVEAALASARLNKKTALINLYKDRIAAMPCNPSVGGPAKGIVVRE
CCCCEEEEEECCCCHHHHHHHHHHHCCCHHHHEEEHHHHHEECCCCCCCCCCCCCEEEEE
IDALGGEMAKAADKTALQTKLLNSSRGPGVALRVQSDKEEYSKYMQKIVEEQINLQLIES
HHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
IATDLIIENNTVTGVLLKDGEILKSKCVVLTTGTYLKSEILTGHEKFSSGPNEEPTSNGI
HHHHEEEECCEEEEEEEECCHHHHCCEEEEEECCHHHHHHHCCHHHHCCCCCCCCCCCCH
SESLKKSGVELFRFKTGTPPRIYKNSVDLSKAIKEPGTDAKLAFSFSTNEYTPIEKQELC
HHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHCCCCCCEEEEEECCCCCCCCCHHHEE
YLIHSSSETKKIIEDNLTKSAMYSGKVESIGPRYCPSFEDKIVRFSSKETHQIFIEPESI
EEEECCCHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCHHHHHEECCCCCCEEEEEECCCC
SGDAYIQGFSTSMPTDVQLLMIRSLPGFEKAEVKTWAYAIEYDCVNPMQLNPSLEIKNIN
CCCEEEEECCCCCCCCEEEEEEECCCCCCHHHEEEEEEEEEECCCCCEECCCCEEEECCC
NLFTAGQINGTSGYEEAAGQGLIAGINASRKIDGLDPLILRRDEAYIGVMIDDLINKGVW
CEEECCCCCCCCCHHHHCCCCEEEECCCCCCCCCCCCEEEECCCCEEEEEHHHHHCCCCC
EPYRLLTSRAEHRLLLRNDNAEHRLKEKGYEIGLIKQAEYNEYLKYTEEINNAIKELEEI
CHHHHHHHHHHCEEEEECCCHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHH
RFTPKSELAIKLKELGQADLSHGYSGIEILKIPTVDIEVMIPYISSLQNLKNNQLQSIVI
CCCCCHHHHHHHHHHCCHHHHCCCCCEEEEEECCCEEEEHHHHHHHHHHHCCCCHHEEEE
ETRFAGYVKTERKTVERLVKLEKKKIPSDIDYDKVENLATEARQKLKKVKPLNIGQASRI
EHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
TGVNPADIQMLLFYLKNNYATEQK
CCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA