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The map label for this gene is deoD [H]

Identifier: 50365480

GI number: 50365480

Start: 771767

End: 772474

Strand: Reverse

Name: deoD [H]

Synonym: Mfl665

Alternate gene names: 50365480

Gene position: 772474-771767 (Counterclockwise)

Preceding gene: 50365481

Following gene: 50365479

Centisome position: 97.38

GC content: 29.66

Gene sequence:

>708_bases
ATGACACCACACATTAGTGCAAAAAAAGAAGATATTGCAAAAACTGTTTTAATGCCAGGAGACCCATTAAGAGCTAAGAA
AATTGCTGAAACATATTTAGAAAATGTTAAGTTAGTTAACGAAGTTAGAAATATGTTCATGTACACAGGAACATATAAAG
GTATACAAGTAACAATAGCAGGAAGCGGAATGGGCTGCCCAAGTATTGGAATATATTCATATGAGCTATTCAATTTTTAT
AATGTAGAAAACATTATTAGAATTGGTTCAGCTGGAAGTTACAAAGAAGAAATCAAAGTTTATGATGTTTACAATGTAAA
AGAAGCATATGGAGATAGTAACTATGCTAAATTAGCAGCAAATATTGATGATAAAGTTATTAAAGCAGGAGATAAACTTT
ATTCATTGATTGAAAAAATTGCTAAAGATAAAAAAATTAAAACATTAACTGGAAGAGCACATTCAGCAGATGTATTTTAC
CGTCATGATGATATTACTGAATTTGTAAAAGAAAACAATTTAGATGTTGTTGAAATGGAATCTGTTGCTTTATTTGCAAA
TGCAATAAAAGCTAAAAAACAAGCTGCATGTTTATTAACAATATCAGATAACTTGGTAACAAAAGAGGTTACGACAGCCG
AAGAAAGACAAAACAATTTTATGCAAATGGTTGAAATTGCTTTAGATGCTGCTGTTGAATCTAACTAA

Upstream 100 bases:

>100_bases
ATGGTTATTGCATCAAAATGATCATCACCGCCACAAGCAAGTGGTGTACCATTTGATAAAACTTTAAGATAAAATAATAT
TAGAAAAAAGAGGAATTATT

Downstream 100 bases:

>100_bases
AAAATAATACTTTTTAAAGGCACATTTATATGTGTCTTTTTACTTGGACTTGTTTTGCCTCTATTTTTTTGATATATTTT
TTTATAACTAACTTAAAAAA

Product: purine-nucleoside phosphorylase

Products: NA

Alternate protein names: PNP [H]

Number of amino acids: Translated: 235; Mature: 234

Protein sequence:

>235_residues
MTPHISAKKEDIAKTVLMPGDPLRAKKIAETYLENVKLVNEVRNMFMYTGTYKGIQVTIAGSGMGCPSIGIYSYELFNFY
NVENIIRIGSAGSYKEEIKVYDVYNVKEAYGDSNYAKLAANIDDKVIKAGDKLYSLIEKIAKDKKIKTLTGRAHSADVFY
RHDDITEFVKENNLDVVEMESVALFANAIKAKKQAACLLTISDNLVTKEVTTAEERQNNFMQMVEIALDAAVESN

Sequences:

>Translated_235_residues
MTPHISAKKEDIAKTVLMPGDPLRAKKIAETYLENVKLVNEVRNMFMYTGTYKGIQVTIAGSGMGCPSIGIYSYELFNFY
NVENIIRIGSAGSYKEEIKVYDVYNVKEAYGDSNYAKLAANIDDKVIKAGDKLYSLIEKIAKDKKIKTLTGRAHSADVFY
RHDDITEFVKENNLDVVEMESVALFANAIKAKKQAACLLTISDNLVTKEVTTAEERQNNFMQMVEIALDAAVESN
>Mature_234_residues
TPHISAKKEDIAKTVLMPGDPLRAKKIAETYLENVKLVNEVRNMFMYTGTYKGIQVTIAGSGMGCPSIGIYSYELFNFYN
VENIIRIGSAGSYKEEIKVYDVYNVKEAYGDSNYAKLAANIDDKVIKAGDKLYSLIEKIAKDKKIKTLTGRAHSADVFYR
HDDITEFVKENNLDVVEMESVALFANAIKAKKQAACLLTISDNLVTKEVTTAEERQNNFMQMVEIALDAAVESN

Specific function: Cleavage Of Guanosine Or Inosine To Respective Bases And Sugar-1-Phosphate Molecules. [C]

COG id: COG0813

COG function: function code F; Purine-nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/UDP phosphorylase family [H]

Homologues:

Organism=Escherichia coli, GI1790844, Length=233, Percent_Identity=39.9141630901288, Blast_Score=160, Evalue=7e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004402
- InterPro:   IPR018017
- InterPro:   IPR018016
- InterPro:   IPR000845 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =2.4.2.1 [H]

Molecular weight: Translated: 26235; Mature: 26104

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS01232 PNP_UDP_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPHISAKKEDIAKTVLMPGDPLRAKKIAETYLENVKLVNEVRNMFMYTGTYKGIQVTIA
CCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEE
GSGMGCPSIGIYSYELFNFYNVENIIRIGSAGSYKEEIKVYDVYNVKEAYGDSNYAKLAA
CCCCCCCCCCEEEEHHHHHCCHHHHEEECCCCCCHHHEEEEEEECHHHHHCCCCCEEEEC
NIDDKVIKAGDKLYSLIEKIAKDKKIKTLTGRAHSADVFYRHDDITEFVKENNLDVVEME
CCCHHHHHCCHHHHHHHHHHHCCCCEEEECCCCCCCEEEEECCHHHHHHHHCCCCEEEHH
SVALFANAIKAKKQAACLLTISDNLVTKEVTTAEERQNNFMQMVEIALDAAVESN
HHHHHHHHHHHHCCEEEEEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TPHISAKKEDIAKTVLMPGDPLRAKKIAETYLENVKLVNEVRNMFMYTGTYKGIQVTIA
CCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEE
GSGMGCPSIGIYSYELFNFYNVENIIRIGSAGSYKEEIKVYDVYNVKEAYGDSNYAKLAA
CCCCCCCCCCEEEEHHHHHCCHHHHEEECCCCCCHHHEEEEEEECHHHHHCCCCCEEEEC
NIDDKVIKAGDKLYSLIEKIAKDKKIKTLTGRAHSADVFYRHDDITEFVKENNLDVVEME
CCCHHHHHCCHHHHHHHHHHHCCCCEEEECCCCCCCEEEEECCHHHHHHHHCCCCEEEHH
SVALFANAIKAKKQAACLLTISDNLVTKEVTTAEERQNNFMQMVEIALDAAVESN
HHHHHHHHHHHHCCEEEEEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7569993 [H]