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The map label for this gene is engD [H]

Identifier: 50365475

GI number: 50365475

Start: 767322

End: 768416

Strand: Reverse

Name: engD [H]

Synonym: Mfl660

Alternate gene names: 50365475

Gene position: 768416-767322 (Counterclockwise)

Preceding gene: 50365476

Following gene: 50365474

Centisome position: 96.87

GC content: 30.14

Gene sequence:

>1095_bases
ATGAGTTTAAAAGTAGGAATAGTAGGTTTACCAAATGTAGGTAAGTCAACTTTATTTAATGCAATAACAAATTCAAATGT
AGAAGCAGCAAATTATCCATTTGCAACAATAGAACCAAACGTAGGTGTTGTTGAAGTTCCAGATGAAAGATTAGATAAAA
TTAATGAAATATTTAATTCAAAGAAAAAAATAGCTACGACAATTGAATTTGTTGACATTGCAGGTTTAATTGCAGGAGCA
TCAAAAGGTGAAGGTTTGGGTAATGCATTTTTAGCTAACATTAGAGAGACTGATGCAATTTGTGAAGTTGTTAGATGTTT
TGAAAATAAAGATATAACACACGTTGAAGGAAGCGTTGACCCTATAAGAGATATTGAAATCATAAACTTAGAATTAATGT
TATCTGATGAAGCTACAATTAAAAAACGTATTGAAAGAATAACTCCAAAGGTTCGTGGTGGAGATAAAACTTTAGTAGCT
GAAATGGAAGTTCTAAAAAAGGCTGCTGAATGTTTAGATTCAAATAAACTTTTAAATACATTAGAATTAAATGATGAAGA
AGATAAAATTCTTAAAGGTTTCCAATTCTTAACTGCAAAAACTTTCATTTATGTTGCTAATGTTAATGATGATGAATTAA
TTGAAGACAATGAATATGTTAAACAAGTTAAAGAATTTGCTTTAAATAATAACGCTTCAGTTGTAAAAATTTGTGCCAAA
ATTGAAGAAGATTTAAGTGAAGCAACACCTGAAGAAAAGGCTGAGTTTTTAAATGACTTAGGTGTTAAATATTCTGGTTT
AGAACAAGTTATAAAAGCGGCATATGAAGCACTAGACTTAGAAACATATTTTACAGCAGGACCACAAGAAGCTAGAAGCT
GACAATATAAAAAAGGATGAACAGCACCTCAATGTGCTGGTGTAATACACACTGACTTTGAAAGAGGATTCATTAAAGCA
GATATTTATAATGTTCAAGACCTTATTGAATTAGGGGATGAAAAGAAAGTTAAAGAATCTGGAAAAATGCGTCTTGAAGG
TAAAGGTTACATAATGCAAGACGGTGATGTTTGTTTCTTTAAATTTAATGTCTAG

Upstream 100 bases:

>100_bases
AAATTTAAAAGTACTGACAAGTTTGATTTATTTATAGAATTAAGAAGAAGTGTTTTAGATAAACAAATAAAACAAATAAT
AAAAAAAGAAGGAGAATAAG

Downstream 100 bases:

>100_bases
TTTAAAAATTAAAAAAACGTTGTCTTATCAAGGCAATAATTTAAGCTATACAATTACATATAAAGAACAGAAACACATCA
TTTTAAGAGTTATACAAGGC

Product: GTP-dependent nucleic acid-binding protein EngD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MSLKVGIVGLPNVGKSTLFNAITNSNVEAANYPFATIEPNVGVVEVPDERLDKINEIFNSKKKIATTIEFVDIAGLIAGA
SKGEGLGNAFLANIRETDAICEVVRCFENKDITHVEGSVDPIRDIEIINLELMLSDEATIKKRIERITPKVRGGDKTLVA
EMEVLKKAAECLDSNKLLNTLELNDEEDKILKGFQFLTAKTFIYVANVNDDELIEDNEYVKQVKEFALNNNASVVKICAK
IEEDLSEATPEEKAEFLNDLGVKYSGLEQVIKAAYEALDLETYFTAGPQEARSWQYKKGWTAPQCAGVIHTDFERGFIKA
DIYNVQDLIELGDEKKVKESGKMRLEGKGYIMQDGDVCFFKFNV

Sequences:

>Translated_364_residues
MSLKVGIVGLPNVGKSTLFNAITNSNVEAANYPFATIEPNVGVVEVPDERLDKINEIFNSKKKIATTIEFVDIAGLIAGA
SKGEGLGNAFLANIRETDAICEVVRCFENKDITHVEGSVDPIRDIEIINLELMLSDEATIKKRIERITPKVRGGDKTLVA
EMEVLKKAAECLDSNKLLNTLELNDEEDKILKGFQFLTAKTFIYVANVNDDELIEDNEYVKQVKEFALNNNASVVKICAK
IEEDLSEATPEEKAEFLNDLGVKYSGLEQVIKAAYEALDLETYFTAGPQEARS*QYKKG*TAPQCAGVIHTDFERGFIKA
DIYNVQDLIELGDEKKVKESGKMRLEGKGYIMQDGDVCFFKFNV
>Mature_363_residues
SLKVGIVGLPNVGKSTLFNAITNSNVEAANYPFATIEPNVGVVEVPDERLDKINEIFNSKKKIATTIEFVDIAGLIAGAS
KGEGLGNAFLANIRETDAICEVVRCFENKDITHVEGSVDPIRDIEIINLELMLSDEATIKKRIERITPKVRGGDKTLVAE
MEVLKKAAECLDSNKLLNTLELNDEEDKILKGFQFLTAKTFIYVANVNDDELIEDNEYVKQVKEFALNNNASVVKICAKI
EEDLSEATPEEKAEFLNDLGVKYSGLEQVIKAAYEALDLETYFTAGPQEARS*QYKKG*TAPQCAGVIHTDFERGFIKAD
IYNVQDLIELGDEKKVKESGKMRLEGKGYIMQDGDVCFFKFNV

Specific function: GTP-dependent nucleic acid-binding protein which may act as a translation factor [H]

COG id: COG0012

COG function: function code J; Predicted GTPase, probable translation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI58761500, Length=369, Percent_Identity=46.3414634146341, Blast_Score=311, Evalue=4e-85,
Organism=Homo sapiens, GI58761502, Length=227, Percent_Identity=38.7665198237885, Blast_Score=151, Evalue=9e-37,
Organism=Homo sapiens, GI24308117, Length=113, Percent_Identity=35.3982300884956, Blast_Score=72, Evalue=6e-13,
Organism=Escherichia coli, GI1787454, Length=364, Percent_Identity=53.2967032967033, Blast_Score=383, Evalue=1e-107,
Organism=Escherichia coli, GI1789574, Length=128, Percent_Identity=32.8125, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17509631, Length=366, Percent_Identity=43.1693989071038, Blast_Score=290, Evalue=9e-79,
Organism=Saccharomyces cerevisiae, GI6319499, Length=370, Percent_Identity=40, Blast_Score=255, Evalue=7e-69,
Organism=Saccharomyces cerevisiae, GI6321773, Length=378, Percent_Identity=37.037037037037, Blast_Score=208, Evalue=1e-54,
Organism=Saccharomyces cerevisiae, GI6321649, Length=218, Percent_Identity=34.4036697247706, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24640873, Length=368, Percent_Identity=42.1195652173913, Blast_Score=285, Evalue=4e-77,
Organism=Drosophila melanogaster, GI24640877, Length=368, Percent_Identity=42.1195652173913, Blast_Score=285, Evalue=4e-77,
Organism=Drosophila melanogaster, GI24640875, Length=368, Percent_Identity=42.1195652173913, Blast_Score=285, Evalue=4e-77,
Organism=Drosophila melanogaster, GI24640879, Length=326, Percent_Identity=40.1840490797546, Blast_Score=232, Evalue=3e-61,
Organism=Drosophila melanogaster, GI24585318, Length=138, Percent_Identity=33.3333333333333, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR004396
- InterPro:   IPR013029
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR012676
- InterPro:   IPR023192 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF06071 YchF-GTPase_C [H]

EC number: NA

Molecular weight: Translated: 40104; Mature: 39973

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLKVGIVGLPNVGKSTLFNAITNSNVEAANYPFATIEPNVGVVEVPDERLDKINEIFNS
CCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCEEECCHHHHHHHHHHHCC
KKKIATTIEFVDIAGLIAGASKGEGLGNAFLANIRETDAICEVVRCFENKDITHVEGSVD
CHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCC
PIRDIEIINLELMLSDEATIKKRIERITPKVRGGDKTLVAEMEVLKKAAECLDSNKLLNT
CCCCEEEEEEEEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHEEE
LELNDEEDKILKGFQFLTAKTFIYVANVNDDELIEDNEYVKQVKEFALNNNASVVKICAK
EECCCCHHHHHHHHHHHEEEEEEEEEECCCCHHCCCHHHHHHHHHHHCCCCCHHHHHHHH
IEEDLSEATPEEKAEFLNDLGVKYSGLEQVIKAAYEALDLETYFTAGPQEARSQYKKGTA
HHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHCCCC
PQCAGVIHTDFERGFIKADIYNVQDLIELGDEKKVKESGKMRLEGKGYIMQDGDVCFFKF
CCCCCCEECCCCCCEEEEHHHHHHHHHHCCCCHHHHHCCCEEECCCCEEEECCCEEEEEE
NV
CC
>Mature Secondary Structure 
SLKVGIVGLPNVGKSTLFNAITNSNVEAANYPFATIEPNVGVVEVPDERLDKINEIFNS
CEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCEEECCHHHHHHHHHHHCC
KKKIATTIEFVDIAGLIAGASKGEGLGNAFLANIRETDAICEVVRCFENKDITHVEGSVD
CHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCC
PIRDIEIINLELMLSDEATIKKRIERITPKVRGGDKTLVAEMEVLKKAAECLDSNKLLNT
CCCCEEEEEEEEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHEEE
LELNDEEDKILKGFQFLTAKTFIYVANVNDDELIEDNEYVKQVKEFALNNNASVVKICAK
EECCCCHHHHHHHHHHHEEEEEEEEEECCCCHHCCCHHHHHHHHHHHCCCCCHHHHHHHH
IEEDLSEATPEEKAEFLNDLGVKYSGLEQVIKAAYEALDLETYFTAGPQEARSQYKKGTA
HHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHCCCC
PQCAGVIHTDFERGFIKADIYNVQDLIELGDEKKVKESGKMRLEGKGYIMQDGDVCFFKF
CCCCCCEECCCCCCEEEEHHHHHHHHHHCCCCHHHHHCCCEEECCCCEEEECCCEEEEEE
NV
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]