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The map label for this gene is pyrG [H]

Identifier: 50365463

GI number: 50365463

Start: 752875

End: 754473

Strand: Reverse

Name: pyrG [H]

Synonym: Mfl648

Alternate gene names: 50365463

Gene position: 754473-752875 (Counterclockwise)

Preceding gene: 50365464

Following gene: 50365461

Centisome position: 95.11

GC content: 29.46

Gene sequence:

>1599_bases
ATGGCAAAATTTGTGTTTGTAACAGGTGGAGTGGTTTCTGGCTTAGGTAAAGGAATTACTGCAAGTTCATTAGGTAATTT
ATTAAAAGCGAGCGGGTTAAAAGTGTTTATGCAAAAATTTGACCCTTATTTAAATGTTGACCCAGGAACAATGAGCCCAT
ATCAACATGGAGAAGTTTTCGTAACTGATGATGGTGGAGAAACTGATTTAGATTTAGGTCACTATGAAAGATTTATAGAT
GAAAAGTTAACAAAAATGTCTTCAACTTCAGCAGGTAAAATTTACTTAGAAACAATTAATGCTGAAAGACGTGGGGATTG
AGGCGGACAAACTATTCAAGTAGTTCCGCACATAACTGATTCAATTAAAAACAAAGTTTACCAAGCTGCTAAAACATCAG
GAGCTGACGTTATCATTTCAGAAATTGGTGGTACTGTTGGTGATATTGAATCTCAACCATTCATTGAAGCTATTAGACAA
ATTAGAATGGAACAAGGAAAAGAAAATGTTGTGTTTATTCATGTTGCGTTACTTTTATATCTAAATGCATCAAAAGAATA
CAAAACAAAACCTATTCAAATGTCAGTTAAAGAATTATTAAGTTTAGGAATCCAACCTGATATTATTGTTCAAAGAACTG
ATAAACATTCTTCAAAAGAAATTAAAGAAAAAATTTCATTATTTTGTAACATCCCAACATCTAATGTTATTGATGCAATT
GATAAAGAATCAATATATGAAGTTCCTTTAGAAATGTATGAGCAAAATTTACATGGTTTAGTTTTAGATCAATTACAAAT
TAGAACTAAAAAAACAGATATGGAATCTTGAATAAAATTCTGTGAAAAAATAAAAGCATCAAAAGAAGAATTCGAAGTTA
CTTTTGTAGGAAAATATATTGAATTACAAGATGCATATTTATCAGTTATCGAATCTTTAAAAATTGCAGGATATGAATTT
AAAAGAAAACTAAAAATCAATTGAATTCAAGCCGATAAATTAAATGAATCAAACTATAAAGAAGTTTTACAAAATGCAAA
AGGTATTTTGGTTCCTGGAGGTTTTGGAGACAGAGGTATCGAAGGAATGATTTTAGCTTCTAAATTTGCTAGAGAAAATA
GCATTCCGTATTTAGGTATTTGTTTAGGGATGCAAATTGCAACTATTTCAATCGCTAGAGATTGATTGAATTTACCAGAA
GCTAATTCAACTGAATTTGATCACTCAGGAGCTGTTCCTATCTTTGACTTCATTAGAGGAATAGATGTTCAAAATTTAGG
TGGTACATTAAGATTAGGAGCATTCTATAAAACAACAATTAAAGAAGGAACAAAAGCAGAAAAACTTTATGGAAGTAGAG
AAGTTTTTGAAAGACATAGACATAGATATGAATTCAATAACGAATACAAAAAAGCATTAGAAGAAAAAGGTTTAGTGTTT
TCAGGTATTTATGAAGAAAAAAAATTAGTAGAAATTATTGAATTACCAAATCATCCTTTTTTTGTTGGAAGTCAATATCA
TCCAGAGTTTACTTCAAGACCTAATAAACCAAATCCATTGTTCAAAGGTTTTGTTGAAGCTATAGTTAATAATAAATAA

Upstream 100 bases:

>100_bases
CAATGACGATGAAGAAGATAGTATGTAATAATTAAAGAAAAGTTGCGACTTTTATGTTTTAGCAACTTTTTTTATTTTTT
AAAAAGAAGGAGATAGTATT

Downstream 100 bases:

>100_bases
TAAAAAAAATCTCTCGCGAGATTTTTTATTTTATTCAAAATCTTTTAATGGTTTTGTCTCCATTAATTCTAGAGTCCTCA
TAAATTCCACCGTTGTTTAA

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 532; Mature: 531

Protein sequence:

>532_residues
MAKFVFVTGGVVSGLGKGITASSLGNLLKASGLKVFMQKFDPYLNVDPGTMSPYQHGEVFVTDDGGETDLDLGHYERFID
EKLTKMSSTSAGKIYLETINAERRGDWGGQTIQVVPHITDSIKNKVYQAAKTSGADVIISEIGGTVGDIESQPFIEAIRQ
IRMEQGKENVVFIHVALLLYLNASKEYKTKPIQMSVKELLSLGIQPDIIVQRTDKHSSKEIKEKISLFCNIPTSNVIDAI
DKESIYEVPLEMYEQNLHGLVLDQLQIRTKKTDMESWIKFCEKIKASKEEFEVTFVGKYIELQDAYLSVIESLKIAGYEF
KRKLKINWIQADKLNESNYKEVLQNAKGILVPGGFGDRGIEGMILASKFARENSIPYLGICLGMQIATISIARDWLNLPE
ANSTEFDHSGAVPIFDFIRGIDVQNLGGTLRLGAFYKTTIKEGTKAEKLYGSREVFERHRHRYEFNNEYKKALEEKGLVF
SGIYEEKKLVEIIELPNHPFFVGSQYHPEFTSRPNKPNPLFKGFVEAIVNNK

Sequences:

>Translated_532_residues
MAKFVFVTGGVVSGLGKGITASSLGNLLKASGLKVFMQKFDPYLNVDPGTMSPYQHGEVFVTDDGGETDLDLGHYERFID
EKLTKMSSTSAGKIYLETINAERRGD*GGQTIQVVPHITDSIKNKVYQAAKTSGADVIISEIGGTVGDIESQPFIEAIRQ
IRMEQGKENVVFIHVALLLYLNASKEYKTKPIQMSVKELLSLGIQPDIIVQRTDKHSSKEIKEKISLFCNIPTSNVIDAI
DKESIYEVPLEMYEQNLHGLVLDQLQIRTKKTDMES*IKFCEKIKASKEEFEVTFVGKYIELQDAYLSVIESLKIAGYEF
KRKLKIN*IQADKLNESNYKEVLQNAKGILVPGGFGDRGIEGMILASKFARENSIPYLGICLGMQIATISIARD*LNLPE
ANSTEFDHSGAVPIFDFIRGIDVQNLGGTLRLGAFYKTTIKEGTKAEKLYGSREVFERHRHRYEFNNEYKKALEEKGLVF
SGIYEEKKLVEIIELPNHPFFVGSQYHPEFTSRPNKPNPLFKGFVEAIVNNK
>Mature_531_residues
AKFVFVTGGVVSGLGKGITASSLGNLLKASGLKVFMQKFDPYLNVDPGTMSPYQHGEVFVTDDGGETDLDLGHYERFIDE
KLTKMSSTSAGKIYLETINAERRGD*GGQTIQVVPHITDSIKNKVYQAAKTSGADVIISEIGGTVGDIESQPFIEAIRQI
RMEQGKENVVFIHVALLLYLNASKEYKTKPIQMSVKELLSLGIQPDIIVQRTDKHSSKEIKEKISLFCNIPTSNVIDAID
KESIYEVPLEMYEQNLHGLVLDQLQIRTKKTDMES*IKFCEKIKASKEEFEVTFVGKYIELQDAYLSVIESLKIAGYEFK
RKLKIN*IQADKLNESNYKEVLQNAKGILVPGGFGDRGIEGMILASKFARENSIPYLGICLGMQIATISIARD*LNLPEA
NSTEFDHSGAVPIFDFIRGIDVQNLGGTLRLGAFYKTTIKEGTKAEKLYGSREVFERHRHRYEFNNEYKKALEEKGLVFS
GIYEEKKLVEIIELPNHPFFVGSQYHPEFTSRPNKPNPLFKGFVEAIVNNK

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI148491070, Length=547, Percent_Identity=44.9725776965265, Blast_Score=446, Evalue=1e-125,
Organism=Homo sapiens, GI28559085, Length=550, Percent_Identity=44.1818181818182, Blast_Score=434, Evalue=1e-121,
Organism=Homo sapiens, GI28559083, Length=550, Percent_Identity=44.1818181818182, Blast_Score=434, Evalue=1e-121,
Organism=Homo sapiens, GI221316689, Length=550, Percent_Identity=44.1818181818182, Blast_Score=434, Evalue=1e-121,
Organism=Escherichia coli, GI1789142, Length=536, Percent_Identity=49.6268656716418, Blast_Score=494, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI25148299, Length=599, Percent_Identity=37.228714524207, Blast_Score=390, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6319432, Length=560, Percent_Identity=44.4642857142857, Blast_Score=441, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6322563, Length=568, Percent_Identity=43.1338028169014, Blast_Score=437, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24664469, Length=549, Percent_Identity=45.1730418943534, Blast_Score=437, Evalue=1e-123,
Organism=Drosophila melanogaster, GI21357815, Length=494, Percent_Identity=43.3198380566802, Blast_Score=362, Evalue=1e-100,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 59067; Mature: 58935

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKFVFVTGGVVSGLGKGITASSLGNLLKASGLKVFMQKFDPYLNVDPGTMSPYQHGEVF
CCEEEEEECHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCEEE
VTDDGGETDLDLGHYERFIDEKLTKMSSTSAGKIYLETINAERRGDGGQTIQVVPHITDS
EECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCEEEEECCHHHH
IKNKVYQAAKTSGADVIISEIGGTVGDIESQPFIEAIRQIRMEQGKENVVFIHVALLLYL
HHHHHHHHHHCCCCEEEHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEHHHHHHHHH
NASKEYKTKPIQMSVKELLSLGIQPDIIVQRTDKHSSKEIKEKISLFCNIPTSNVIDAID
CCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHEEECCCCHHHHHHHC
KESIYEVPLEMYEQNLHGLVLDQLQIRTKKTDMESIKFCEKIKASKEEFEVTFVGKYIEL
HHHHHHCCHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHEEEEEEEEHHHH
QDAYLSVIESLKIAGYEFKRKLKINIQADKLNESNYKEVLQNAKGILVPGGFGDRGIEGM
HHHHHHHHHHHHHCCEEEEEEEEEEEEECCCCCCHHHHHHHCCCCEEEECCCCCCCCCCE
ILASKFARENSIPYLGICLGMQIATISIARDLNLPEANSTEFDHSGAVPIFDFIRGIDVQ
EEHHHHHHCCCCCEEEHHHCCHHEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCCC
NLGGTLRLGAFYKTTIKEGTKAEKLYGSREVFERHRHRYEFNNEYKKALEEKGLVFSGIY
CCCCEEEECHHHHHHHHCCCHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHCCCEEECCH
EEKKLVEIIELPNHPFFVGSQYHPEFTSRPNKPNPLFKGFVEAIVNNK
HHHHHHHHHHCCCCCEEECCCCCCHHCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKFVFVTGGVVSGLGKGITASSLGNLLKASGLKVFMQKFDPYLNVDPGTMSPYQHGEVF
CEEEEEECHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCEEE
VTDDGGETDLDLGHYERFIDEKLTKMSSTSAGKIYLETINAERRGDGGQTIQVVPHITDS
EECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCEEEEECCHHHH
IKNKVYQAAKTSGADVIISEIGGTVGDIESQPFIEAIRQIRMEQGKENVVFIHVALLLYL
HHHHHHHHHHCCCCEEEHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEHHHHHHHHH
NASKEYKTKPIQMSVKELLSLGIQPDIIVQRTDKHSSKEIKEKISLFCNIPTSNVIDAID
CCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHEEECCCCHHHHHHHC
KESIYEVPLEMYEQNLHGLVLDQLQIRTKKTDMESIKFCEKIKASKEEFEVTFVGKYIEL
HHHHHHCCHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHEEEEEEEEHHHH
QDAYLSVIESLKIAGYEFKRKLKINIQADKLNESNYKEVLQNAKGILVPGGFGDRGIEGM
HHHHHHHHHHHHHCCEEEEEEEEEEEEECCCCCCHHHHHHHCCCCEEEECCCCCCCCCCE
ILASKFARENSIPYLGICLGMQIATISIARDLNLPEANSTEFDHSGAVPIFDFIRGIDVQ
EEHHHHHHCCCCCEEEHHHCCHHEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCCC
NLGGTLRLGAFYKTTIKEGTKAEKLYGSREVFERHRHRYEFNNEYKKALEEKGLVFSGIY
CCCCEEEECHHHHHHHHCCCHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHCCCEEECCH
EEKKLVEIIELPNHPFFVGSQYHPEFTSRPNKPNPLFKGFVEAIVNNK
HHHHHHHHHHCCCCCEEECCCCCCHHCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA