Click here to switch to the map view.

The map label for this gene is prfA [H]

Identifier: 50365449

GI number: 50365449

Start: 739872

End: 740963

Strand: Reverse

Name: prfA [H]

Synonym: Mfl634

Alternate gene names: 50365449

Gene position: 740963-739872 (Counterclockwise)

Preceding gene: 50365450

Following gene: 50365448

Centisome position: 93.41

GC content: 32.69

Gene sequence:

>1092_bases
ATGAATCCAAAAACTTATGAAGCATTGGAAACAATGCAAAAAAGGGTTGATCAAATAGATAAAGATTTACAATCAGAAGA
AATTGTTTCAGATGTTAAAAAAATGTTAGAACTTAACAAAGAAAGAGCAAATTTAATCGAAGTTGTAGAAAAATTTATTG
AATATAAGACGATAATTCAATCAATTGCTGATGCTAAAGAAATATTAGGTAATGAAAAAGATGCTGAAATGATTGAACTA
GCTAAAATGGAATTATCAGAAAATGAAGATGCTGTTGAACCAATTGTCGCAATTATTGAGGAATTGCTTTTACCAAAAGA
CCCCAACGATGACAAAAACGTTATTGTAGAAATTAGAGGTGCAGCAGGAGGAGATGAAGCAAATATATTTGCTGGTGATT
TACTAAGAATGTATAAACTATATGCTGAAACTCAAAATTGAAAAATTACAATGCTTGATGCAAACTCTTCAGAAGCTGGT
GGCTTCTCACAAGTTTCTTTTATGGTTAAAGGTGACCGAGTTTATTCAAAATTAAAATTTGAATCTGGAGCCCACAGAGT
ACAAAGAGTACCAAAAACTGAAGCTAAAGGTAGAATTCAAACTTCTACTGCTACAGTTGCTGTTTTACCTGAAATGAGTG
ATGTTGAAATTGAAATCAAAAACAGTGATTTAAGAATTGATACGTATAGATCATCTGGAGCTGGTGGTCAGCACGTTAAC
ACAACAGATTCAGCTGTTCGTATTACTCATATACCAACTGGTGTTGTTGCAGCTTCTCAAGATGGAAGAAGTCAACATGA
TAACAAAGATATAGCTATGACAATGCTAAGAGCAAGAATATATGAAGCAGAATTAGAGAAACAACAAGCAGAAGCTGATG
CAACACGTAAGAACGCTGTTGGTACAGGAGCTAGAAGTGAAAAAATAAGAACATACAATTATCCACAAAATCGTGTAACT
GATCATAGAGTAGGTTTAACACTAAACAAACTGGATCAAGTTATGGAAGGAAAAATTGATGATTTTATTATTGCCTTAGT
TAATGATGAACAACGTCAGAAAGTTGAAGCACAATTACAAGATAATGAATAA

Upstream 100 bases:

>100_bases
GCAAACCAGCTGATTGAAATTCACCATTAATACTAGTTGATGGTAATGATAGTTACGAAGCTAGATGTAGAAATTGCTAC
CAAATAGAAAAGGGGTAAAA

Downstream 100 bases:

>100_bases
TGCAAATATAAAAATTGCTTTAGATTTTCTTTTAGAAAATAGTAAAATTGGAAAATCGGATGCTATTGAAATTATTTCAT
TTATTACAAAAATTGAATAC

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MNPKTYEALETMQKRVDQIDKDLQSEEIVSDVKKMLELNKERANLIEVVEKFIEYKTIIQSIADAKEILGNEKDAEMIEL
AKMELSENEDAVEPIVAIIEELLLPKDPNDDKNVIVEIRGAAGGDEANIFAGDLLRMYKLYAETQNWKITMLDANSSEAG
GFSQVSFMVKGDRVYSKLKFESGAHRVQRVPKTEAKGRIQTSTATVAVLPEMSDVEIEIKNSDLRIDTYRSSGAGGQHVN
TTDSAVRITHIPTGVVAASQDGRSQHDNKDIAMTMLRARIYEAELEKQQAEADATRKNAVGTGARSEKIRTYNYPQNRVT
DHRVGLTLNKLDQVMEGKIDDFIIALVNDEQRQKVEAQLQDNE

Sequences:

>Translated_363_residues
MNPKTYEALETMQKRVDQIDKDLQSEEIVSDVKKMLELNKERANLIEVVEKFIEYKTIIQSIADAKEILGNEKDAEMIEL
AKMELSENEDAVEPIVAIIEELLLPKDPNDDKNVIVEIRGAAGGDEANIFAGDLLRMYKLYAETQN*KITMLDANSSEAG
GFSQVSFMVKGDRVYSKLKFESGAHRVQRVPKTEAKGRIQTSTATVAVLPEMSDVEIEIKNSDLRIDTYRSSGAGGQHVN
TTDSAVRITHIPTGVVAASQDGRSQHDNKDIAMTMLRARIYEAELEKQQAEADATRKNAVGTGARSEKIRTYNYPQNRVT
DHRVGLTLNKLDQVMEGKIDDFIIALVNDEQRQKVEAQLQDNE
>Mature_363_residues
MNPKTYEALETMQKRVDQIDKDLQSEEIVSDVKKMLELNKERANLIEVVEKFIEYKTIIQSIADAKEILGNEKDAEMIEL
AKMELSENEDAVEPIVAIIEELLLPKDPNDDKNVIVEIRGAAGGDEANIFAGDLLRMYKLYAETQN*KITMLDANSSEAG
GFSQVSFMVKGDRVYSKLKFESGAHRVQRVPKTEAKGRIQTSTATVAVLPEMSDVEIEIKNSDLRIDTYRSSGAGGQHVN
TTDSAVRITHIPTGVVAASQDGRSQHDNKDIAMTMLRARIYEAELEKQQAEADATRKNAVGTGARSEKIRTYNYPQNRVT
DHRVGLTLNKLDQVMEGKIDDFIIALVNDEQRQKVEAQLQDNE

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=294, Percent_Identity=44.8979591836735, Blast_Score=251, Evalue=7e-67,
Organism=Homo sapiens, GI34577120, Length=341, Percent_Identity=35.1906158357771, Blast_Score=213, Evalue=3e-55,
Organism=Homo sapiens, GI166795305, Length=199, Percent_Identity=43.21608040201, Blast_Score=161, Evalue=9e-40,
Organism=Escherichia coli, GI1787462, Length=359, Percent_Identity=48.1894150417827, Blast_Score=336, Evalue=1e-93,
Organism=Escherichia coli, GI2367172, Length=343, Percent_Identity=35.5685131195335, Blast_Score=207, Evalue=7e-55,
Organism=Caenorhabditis elegans, GI17542784, Length=279, Percent_Identity=35.84229390681, Blast_Score=167, Evalue=8e-42,
Organism=Saccharomyces cerevisiae, GI6321295, Length=372, Percent_Identity=39.5161290322581, Blast_Score=232, Evalue=6e-62,
Organism=Drosophila melanogaster, GI19921226, Length=324, Percent_Identity=39.8148148148148, Blast_Score=224, Evalue=1e-58,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40484; Mature: 40484

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPKTYEALETMQKRVDQIDKDLQSEEIVSDVKKMLELNKERANLIEVVEKFIEYKTIIQ
CCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
SIADAKEILGNEKDAEMIELAKMELSENEDAVEPIVAIIEELLLPKDPNDDKNVIVEIRG
HHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEC
AAGGDEANIFAGDLLRMYKLYAETQNKITMLDANSSEAGGFSQVSFMVKGDRVYSKLKFE
CCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEEEECHHHHHHHHHC
SGAHRVQRVPKTEAKGRIQTSTATVAVLPEMSDVEIEIKNSDLRIDTYRSSGAGGQHVNT
HHHHHHHHCCCCCCCCEEEECCEEEEEECCCCCCEEEEECCCEEEEECCCCCCCCCCCCC
TDSAVRITHIPTGVVAASQDGRSQHDNKDIAMTMLRARIYEAELEKQQAEADATRKNAVG
CCCEEEEEECCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TGARSEKIRTYNYPQNRVTDHRVGLTLNKLDQVMEGKIDDFIIALVNDEQRQKVEAQLQD
CCCCCCCCEECCCCCCCCCCHHHCCCHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHCCC
NE
CC
>Mature Secondary Structure
MNPKTYEALETMQKRVDQIDKDLQSEEIVSDVKKMLELNKERANLIEVVEKFIEYKTIIQ
CCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
SIADAKEILGNEKDAEMIELAKMELSENEDAVEPIVAIIEELLLPKDPNDDKNVIVEIRG
HHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEC
AAGGDEANIFAGDLLRMYKLYAETQNKITMLDANSSEAGGFSQVSFMVKGDRVYSKLKFE
CCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEEEECHHHHHHHHHC
SGAHRVQRVPKTEAKGRIQTSTATVAVLPEMSDVEIEIKNSDLRIDTYRSSGAGGQHVNT
HHHHHHHHCCCCCCCCEEEECCEEEEEECCCCCCEEEEECCCEEEEECCCCCCCCCCCCC
TDSAVRITHIPTGVVAASQDGRSQHDNKDIAMTMLRARIYEAELEKQQAEADATRKNAVG
CCCEEEEEECCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TGARSEKIRTYNYPQNRVTDHRVGLTLNKLDQVMEGKIDDFIIALVNDEQRQKVEAQLQD
CCCCCCCCEECCCCCCCCCCHHHCCCHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHCCC
NE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA