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The map label for this gene is ywkE [H]

Identifier: 50365448

GI number: 50365448

Start: 739019

End: 739921

Strand: Reverse

Name: ywkE [H]

Synonym: Mfl633

Alternate gene names: 50365448

Gene position: 739921-739019 (Counterclockwise)

Preceding gene: 50365449

Following gene: 50365447

Centisome position: 93.28

GC content: 21.15

Gene sequence:

>903_bases
ATGATGAACAACGTCAGAAAGTTGAAGCACAATTACAAGATAATGAATAATGCAAATATAAAAATTGCTTTAGATTTTCT
TTTAGAAAATAGTAAAATTGGAAAATCGGATGCTATTGAAATTATTTCATTTATTACAAAAATTGAATACTCAGAAGTTT
TATTTTCACAAGAAAAAGTTTTAAATAAAAAACAATTTAAAAAAATAATCAAAATTTCTAAAAAGTTAGCTAAAGGGAAA
CCTTTAGCTTATATTTTAGGATACAAAATTTTTAGGACTCATAAAATTTTAGTAAATAAAAATACTTTAATACCAAGAAT
GGAAACTGAACTTATTGTTGATTATGTTAATGAATTTATCAATTCTCAAAATGAAAAAATTTCAGTATTAGATCTTTGTT
GTGGTTCGGGTTGTATAGGAATTTCTATAGCTATAGAAAACAAAGATAAAATGGAAAATGTTACATTCTCAGATATTTCT
AAAAAAGCGTTAAATATAACTAGTAAAAATATTGAAAATAATAATTTAGTAAATTGAACAAAAGTTGTTAAAAGTGATTT
TTTAAATTCTATTATAAAACAACAAAATAAATTTAACATTCTTGTTTGTAACCCACCATATATTGATTTTAATGATGTTG
ATGTAGATAAAATGACAAAAAAATATGAACCAAAATTAGCACTTTTCGCAAAAGATAATGGATTGTTTTTTTATAAGGAA
GCAATTAAAAATATTGATAAATTTATGGATATTACAAAAAATATACTAATTGTTTTTGAAATAGGGTGAAAACAAGAAAA
AGAGCTAGATGTCTTTTTAAAACAAGAATTAGGTTTAAAATATAAATGAAAGTTTGAAAAAGATTATTTTAATAATTTAA
GATATTTAATACTTACTAAGTAG

Upstream 100 bases:

>100_bases
CCACAAAATCGTGTAACTGATCATAGAGTAGGTTTAACACTAAACAAACTGGATCAAGTTATGGAAGGAAAAATTGATGA
TTTTATTATTGCCTTAGTTA

Downstream 100 bases:

>100_bases
GAGATAAGATGGAAAAAAAGATTGAATTTAAAAGTAAATTCAGACTGAACTTTGCAATAAAAGACAAAGTTGCTTCTGTT
CAAGAAAAAAATGCACACTT

Product: polypeptide chain release factor methylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MMNNVRKLKHNYKIMNNANIKIALDFLLENSKIGKSDAIEIISFITKIEYSEVLFSQEKVLNKKQFKKIIKISKKLAKGK
PLAYILGYKIFRTHKILVNKNTLIPRMETELIVDYVNEFINSQNEKISVLDLCCGSGCIGISIAIENKDKMENVTFSDIS
KKALNITSKNIENNNLVNWTKVVKSDFLNSIIKQQNKFNILVCNPPYIDFNDVDVDKMTKKYEPKLALFAKDNGLFFYKE
AIKNIDKFMDITKNILIVFEIGWKQEKELDVFLKQELGLKYKWKFEKDYFNNLRYLILTK

Sequences:

>Translated_300_residues
MMNNVRKLKHNYKIMNNANIKIALDFLLENSKIGKSDAIEIISFITKIEYSEVLFSQEKVLNKKQFKKIIKISKKLAKGK
PLAYILGYKIFRTHKILVNKNTLIPRMETELIVDYVNEFINSQNEKISVLDLCCGSGCIGISIAIENKDKMENVTFSDIS
KKALNITSKNIENNNLVN*TKVVKSDFLNSIIKQQNKFNILVCNPPYIDFNDVDVDKMTKKYEPKLALFAKDNGLFFYKE
AIKNIDKFMDITKNILIVFEIG*KQEKELDVFLKQELGLKYK*KFEKDYFNNLRYLILTK
>Mature_300_residues
MMNNVRKLKHNYKIMNNANIKIALDFLLENSKIGKSDAIEIISFITKIEYSEVLFSQEKVLNKKQFKKIIKISKKLAKGK
PLAYILGYKIFRTHKILVNKNTLIPRMETELIVDYVNEFINSQNEKISVLDLCCGSGCIGISIAIENKDKMENVTFSDIS
KKALNITSKNIENNNLVN*TKVVKSDFLNSIIKQQNKFNILVCNPPYIDFNDVDVDKMTKKYEPKLALFAKDNGLFFYKE
AIKNIDKFMDITKNILIVFEIG*KQEKELDVFLKQELGLKYK*KFEKDYFNNLRYLILTK

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Escherichia coli, GI1787463, Length=272, Percent_Identity=26.8382352941176, Blast_Score=103, Evalue=9e-24,
Organism=Escherichia coli, GI87082085, Length=205, Percent_Identity=27.8048780487805, Blast_Score=92, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6324266, Length=162, Percent_Identity=31.4814814814815, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 34473; Mature: 34473

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNNVRKLKHNYKIMNNANIKIALDFLLENSKIGKSDAIEIISFITKIEYSEVLFSQEKV
CCCHHHHHHHCEEEEECCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LNKKQFKKIIKISKKLAKGKPLAYILGYKIFRTHKILVNKNTLIPRMETELIVDYVNEFI
HCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHEEEEEEECCCCCCCHHHHHHHHHHHHHH
NSQNEKISVLDLCCGSGCIGISIAIENKDKMENVTFSDISKKALNITSKNIENNNLVNTK
CCCCCEEEEEEEECCCCCEEEEEEECCCHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEHH
VVKSDFLNSIIKQQNKFNILVCNPPYIDFNDVDVDKMTKKYEPKLALFAKDNGLFFYKEA
HHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHCCCCEEEEEECCCEEEHHHH
IKNIDKFMDITKNILIVFEIGKQEKELDVFLKQELGLKYKKFEKDYFNNLRYLILTK
HHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCEEEEEEEC
>Mature Secondary Structure
MMNNVRKLKHNYKIMNNANIKIALDFLLENSKIGKSDAIEIISFITKIEYSEVLFSQEKV
CCCHHHHHHHCEEEEECCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LNKKQFKKIIKISKKLAKGKPLAYILGYKIFRTHKILVNKNTLIPRMETELIVDYVNEFI
HCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHEEEEEEECCCCCCCHHHHHHHHHHHHHH
NSQNEKISVLDLCCGSGCIGISIAIENKDKMENVTFSDISKKALNITSKNIENNNLVNTK
CCCCCEEEEEEEECCCCCEEEEEEECCCHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEHH
VVKSDFLNSIIKQQNKFNILVCNPPYIDFNDVDVDKMTKKYEPKLALFAKDNGLFFYKEA
HHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHCCCCEEEEEECCCEEEHHHH
IKNIDKFMDITKNILIVFEIGKQEKELDVFLKQELGLKYKKFEKDYFNNLRYLILTK
HHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]