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The map label for this gene is fusA [H]

Identifier: 50365438

GI number: 50365438

Start: 729247

End: 731316

Strand: Reverse

Name: fusA [H]

Synonym: Mfl622

Alternate gene names: 50365438

Gene position: 731316-729247 (Counterclockwise)

Preceding gene: 50365439

Following gene: 50365437

Centisome position: 92.2

GC content: 35.51

Gene sequence:

>2070_bases
ATGCCAAGAGAATTTAGTTTAGAAAATACTCGTAACCTTGGAATTATGGCTCACATTGATGCTGGGAAAACTACTACAAC
AGAACGTATTTTATTCCACACAGGTAAAATTCATAAAATTGGTGAAACTCATGAAGGGGCTTCACAAATGGACTGAATGG
CACAAGAGCAAGAACGTGGTATTACAATTACTTCAGCTGCAACAACTGCATTCTGAAAAAATAACCGTTTCAACATAATT
GATACTCCAGGTCACGTTGACTTCACTGTTGAAGTTGAACGTTCATTACGTGTTCTTGATGGAGCTGTAGCAGTTCTTGA
TGGACAATCAGGAGTTGAACCTCAAACTGAAACTGTTTGAAGACAAGCAACTACTTATAGAGTTCCTCGTATTGTTTTTG
TTAACAAAATGGATAAAACTGGAGCAGATTTCATCTATTCAGTTAAATCAATCGGGGATCGTTTAGGAGCCAAAGCTGCT
CCAATTCAATTACCAATCGGTGCTGAAGATAACTTCACAGGAATAATCGATTTAGTTGAAATGAAAGCTTACGAATTTGA
CGGAAAAGCTGAAGAAATTGCTAAAGAAATTGAAATTCCTGCAGATTTAAAAGATCAAGCAGAAATTTTAAGAAGCGAAT
TAGTTGAAGCCGCTGTTGAATACGATGAAGAATTAATGATGAAATTCTTAGATGGTGAAGAAATCACTATTCCTGAATTA
AAACAAGCAATCCGTAAAGGGGTTATTGGTGCAGAATTCTTCCCAGTTTTAGCTGGATCAGCTTTCAAAAACAAAGGTGT
TAAATTATTATTAGACGCAGTTGTTGATTATTTACCTTCACCATTAGACGTTCCTTCAATTAAAGGTGTTTTACCAAATG
GTGAAGAAGCAGAAAGACATGCAGATGACAATGAACCATTCTCAGCTTTAGCTTTCAAAGTTATGACTGACCCATTCGTT
GGTAAATTAACATTCTTTAGAGTTTACTCAGGTATTCTTACAAAAGGAAGTTATGTATTGAACTCAACAAAAGGTGATAA
AGAACGTGTAGGACGTATTTTACAAATGCACGCAAACAACCGTAACGAAATTGAAGAAGTTTATGCTGGAGATATTGCAG
CTGCTGTTGGATTAAAAAATACTACAACAGGTGATACTTTAGTTGATGAAAAACATGAAATTATTTTAGAATCAATGGTA
TTCCCAGAACCAGTTATCCAATTAGCTTTAGAACCAAAAACTAAAGCGGATCAAGAAAAAATGGGATTAGCATTATCAAA
ATTAGCAGAAGAAGATCCAACTTTCAGAACATATACTGATGAAGAAACTGGACAAACTATTATTGCTGGTATGGGTGAAT
TACACTTAGATATTATTGTTGACCGTATGAGACGTGAATTCAAAGTTGAAACAAATGTTGGAGCACCTCAAGTATCATAC
CGTGAAACAATTAAATTACCAGCAAAAGCTGAAGGTAAATATGTTAAACAATCAGGAGGACGTGGGTCATATGGTCACGT
TGTTATTGAATTCGAACCAAACGTTGATAAAGGGTTTGAATGAGTTGACAAAATTACTGGAGGACGTGTTTCTAAAGAAT
ACATCAATGCTGCACGTGTTGGTTTAGAAAACGCACTTACAAACGGGGTTGTTGCTGGATACCCAATGATCGATGTTAAA
GCAACAATCGTTGATGGATCAATGCACGACGTTGACTCAAACGAAATGGCTTATAAAATCGCTGCATCATTTGCTTTAAA
AGAAGCATGTAAAAAAATGAATCCAGTTATCTTAGAACCAATCATGAACGTTGAAGTAACTGTTCCGGATGAATACTATG
GAGATGTAATGGGTAACATTTCATCAAAACGTGGATTAATCGAAGGATCAGAACAAAGAGGAAACGCACAAACAATTAAA
TCTAAAGTTCCTCTAACAGAAATGTTTGGTTATGCAACAGAATTAAGATCATTCACACAAGGACGTGGAAACTACACTAT
GATTTTCAGTCACTATGCTGAAGCACCTAGATCTATTGCAGAAGAAATTATTAAAAAATCAGGTAAGTAG

Upstream 100 bases:

>100_bases
AAACGTGAAGATACACATAAAATGGCAGAAGCTAATAAAGCATTTGCACACTACCGTTGATAAGATCAACAAATATTTTA
TTTAGATAGGGGGCAAAATT

Downstream 100 bases:

>100_bases
TAATTTATAATTGCACTTATTGATGTAAAAAGTTAAAATATTCTTAATTAATAAGAATAAACAACGCCCCTACGGGGGCG
GTAAAGATACTAGAAAGTAA

Product: elongation factor G

Products: GDP; phosphate

Alternate protein names: EF-G [H]

Number of amino acids: Translated: 689; Mature: 688

Protein sequence:

>689_residues
MPREFSLENTRNLGIMAHIDAGKTTTTERILFHTGKIHKIGETHEGASQMDWMAQEQERGITITSAATTAFWKNNRFNII
DTPGHVDFTVEVERSLRVLDGAVAVLDGQSGVEPQTETVWRQATTYRVPRIVFVNKMDKTGADFIYSVKSIGDRLGAKAA
PIQLPIGAEDNFTGIIDLVEMKAYEFDGKAEEIAKEIEIPADLKDQAEILRSELVEAAVEYDEELMMKFLDGEEITIPEL
KQAIRKGVIGAEFFPVLAGSAFKNKGVKLLLDAVVDYLPSPLDVPSIKGVLPNGEEAERHADDNEPFSALAFKVMTDPFV
GKLTFFRVYSGILTKGSYVLNSTKGDKERVGRILQMHANNRNEIEEVYAGDIAAAVGLKNTTTGDTLVDEKHEIILESMV
FPEPVIQLALEPKTKADQEKMGLALSKLAEEDPTFRTYTDEETGQTIIAGMGELHLDIIVDRMRREFKVETNVGAPQVSY
RETIKLPAKAEGKYVKQSGGRGSYGHVVIEFEPNVDKGFEWVDKITGGRVSKEYINAARVGLENALTNGVVAGYPMIDVK
ATIVDGSMHDVDSNEMAYKIAASFALKEACKKMNPVILEPIMNVEVTVPDEYYGDVMGNISSKRGLIEGSEQRGNAQTIK
SKVPLTEMFGYATELRSFTQGRGNYTMIFSHYAEAPRSIAEEIIKKSGK

Sequences:

>Translated_689_residues
MPREFSLENTRNLGIMAHIDAGKTTTTERILFHTGKIHKIGETHEGASQMD*MAQEQERGITITSAATTAF*KNNRFNII
DTPGHVDFTVEVERSLRVLDGAVAVLDGQSGVEPQTETV*RQATTYRVPRIVFVNKMDKTGADFIYSVKSIGDRLGAKAA
PIQLPIGAEDNFTGIIDLVEMKAYEFDGKAEEIAKEIEIPADLKDQAEILRSELVEAAVEYDEELMMKFLDGEEITIPEL
KQAIRKGVIGAEFFPVLAGSAFKNKGVKLLLDAVVDYLPSPLDVPSIKGVLPNGEEAERHADDNEPFSALAFKVMTDPFV
GKLTFFRVYSGILTKGSYVLNSTKGDKERVGRILQMHANNRNEIEEVYAGDIAAAVGLKNTTTGDTLVDEKHEIILESMV
FPEPVIQLALEPKTKADQEKMGLALSKLAEEDPTFRTYTDEETGQTIIAGMGELHLDIIVDRMRREFKVETNVGAPQVSY
RETIKLPAKAEGKYVKQSGGRGSYGHVVIEFEPNVDKGFE*VDKITGGRVSKEYINAARVGLENALTNGVVAGYPMIDVK
ATIVDGSMHDVDSNEMAYKIAASFALKEACKKMNPVILEPIMNVEVTVPDEYYGDVMGNISSKRGLIEGSEQRGNAQTIK
SKVPLTEMFGYATELRSFTQGRGNYTMIFSHYAEAPRSIAEEIIKKSGK
>Mature_688_residues
PREFSLENTRNLGIMAHIDAGKTTTTERILFHTGKIHKIGETHEGASQMD*MAQEQERGITITSAATTAF*KNNRFNIID
TPGHVDFTVEVERSLRVLDGAVAVLDGQSGVEPQTETV*RQATTYRVPRIVFVNKMDKTGADFIYSVKSIGDRLGAKAAP
IQLPIGAEDNFTGIIDLVEMKAYEFDGKAEEIAKEIEIPADLKDQAEILRSELVEAAVEYDEELMMKFLDGEEITIPELK
QAIRKGVIGAEFFPVLAGSAFKNKGVKLLLDAVVDYLPSPLDVPSIKGVLPNGEEAERHADDNEPFSALAFKVMTDPFVG
KLTFFRVYSGILTKGSYVLNSTKGDKERVGRILQMHANNRNEIEEVYAGDIAAAVGLKNTTTGDTLVDEKHEIILESMVF
PEPVIQLALEPKTKADQEKMGLALSKLAEEDPTFRTYTDEETGQTIIAGMGELHLDIIVDRMRREFKVETNVGAPQVSYR
ETIKLPAKAEGKYVKQSGGRGSYGHVVIEFEPNVDKGFE*VDKITGGRVSKEYINAARVGLENALTNGVVAGYPMIDVKA
TIVDGSMHDVDSNEMAYKIAASFALKEACKKMNPVILEPIMNVEVTVPDEYYGDVMGNISSKRGLIEGSEQRGNAQTIKS
KVPLTEMFGYATELRSFTQGRGNYTMIFSHYAEAPRSIAEEIIKKSGK

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=693, Percent_Identity=42.5685425685426, Blast_Score=530, Evalue=1e-150,
Organism=Homo sapiens, GI19923640, Length=723, Percent_Identity=39.4190871369295, Blast_Score=434, Evalue=1e-121,
Organism=Homo sapiens, GI25306287, Length=723, Percent_Identity=37.2060857538036, Blast_Score=385, Evalue=1e-107,
Organism=Homo sapiens, GI25306283, Length=453, Percent_Identity=43.2671081677704, Blast_Score=298, Evalue=1e-80,
Organism=Homo sapiens, GI217272892, Length=794, Percent_Identity=24.0554156171285, Blast_Score=120, Evalue=5e-27,
Organism=Homo sapiens, GI217272894, Length=794, Percent_Identity=24.0554156171285, Blast_Score=120, Evalue=5e-27,
Organism=Homo sapiens, GI4503483, Length=480, Percent_Identity=26.4583333333333, Blast_Score=109, Evalue=8e-24,
Organism=Homo sapiens, GI157426893, Length=143, Percent_Identity=39.8601398601399, Blast_Score=96, Evalue=9e-20,
Organism=Homo sapiens, GI94966754, Length=136, Percent_Identity=38.9705882352941, Blast_Score=91, Evalue=5e-18,
Organism=Homo sapiens, GI310132016, Length=117, Percent_Identity=38.4615384615385, Blast_Score=77, Evalue=7e-14,
Organism=Homo sapiens, GI310110807, Length=117, Percent_Identity=38.4615384615385, Blast_Score=77, Evalue=7e-14,
Organism=Homo sapiens, GI310123363, Length=117, Percent_Identity=38.4615384615385, Blast_Score=77, Evalue=7e-14,
Organism=Escherichia coli, GI1789738, Length=704, Percent_Identity=59.9431818181818, Blast_Score=823, Evalue=0.0,
Organism=Escherichia coli, GI1790835, Length=494, Percent_Identity=29.1497975708502, Blast_Score=158, Evalue=1e-39,
Organism=Escherichia coli, GI48994988, Length=176, Percent_Identity=39.7727272727273, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1788922, Length=157, Percent_Identity=37.5796178343949, Blast_Score=88, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17533571, Length=681, Percent_Identity=42.2907488986784, Blast_Score=503, Evalue=1e-142,
Organism=Caenorhabditis elegans, GI17556745, Length=719, Percent_Identity=29.0681502086231, Blast_Score=295, Evalue=5e-80,
Organism=Caenorhabditis elegans, GI17506493, Length=479, Percent_Identity=25.8872651356994, Blast_Score=104, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17557151, Length=154, Percent_Identity=38.961038961039, Blast_Score=93, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6323098, Length=690, Percent_Identity=42.6086956521739, Blast_Score=541, Evalue=1e-154,
Organism=Saccharomyces cerevisiae, GI6322359, Length=772, Percent_Identity=32.7720207253886, Blast_Score=362, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6324707, Length=496, Percent_Identity=25.8064516129032, Blast_Score=107, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6320593, Length=496, Percent_Identity=25.8064516129032, Blast_Score=107, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6323320, Length=158, Percent_Identity=35.4430379746835, Blast_Score=84, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6324166, Length=144, Percent_Identity=35.4166666666667, Blast_Score=70, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24582462, Length=692, Percent_Identity=41.907514450867, Blast_Score=532, Evalue=1e-151,
Organism=Drosophila melanogaster, GI221458488, Length=729, Percent_Identity=33.0589849108368, Blast_Score=332, Evalue=7e-91,
Organism=Drosophila melanogaster, GI24585711, Length=480, Percent_Identity=25, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24585713, Length=480, Percent_Identity=25, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24585709, Length=480, Percent_Identity=25, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI78706572, Length=147, Percent_Identity=38.0952380952381, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI28574573, Length=142, Percent_Identity=34.5070422535211, Blast_Score=77, Evalue=4e-14,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 75314; Mature: 75182

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPREFSLENTRNLGIMAHIDAGKTTTTERILFHTGKIHKIGETHEGASQMDMAQEQERGI
CCCCCCCCCCCCCEEEEEECCCCCCCHHEEEEECCCEEECCCCCCCHHHHHHHHHHHCCE
TITSAATTAFKNNRFNIIDTPGHVDFTVEVERSLRVLDGAVAVLDGQSGVEPQTETVRQA
EEEECHHHHCCCCCEEEEECCCCEEEEEEHHHHHHHHCCEEEEEECCCCCCCHHHHHHHH
TTYRVPRIVFVNKMDKTGADFIYSVKSIGDRLGAKAAPIQLPIGAEDNFTGIIDLVEMKA
HHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHH
YEFDGKAEEIAKEIEIPADLKDQAEILRSELVEAAVEYDEELMMKFLDGEEITIPELKQA
HCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
IRKGVIGAEFFPVLAGSAFKNKGVKLLLDAVVDYLPSPLDVPSIKGVLPNGEEAERHADD
HHHCCCCHHHHHHHCCCHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCC
NEPFSALAFKVMTDPFVGKLTFFRVYSGILTKGSYVLNSTKGDKERVGRILQMHANNRNE
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCHHH
IEEVYAGDIAAAVGLKNTTTGDTLVDEKHEIILESMVFPEPVIQLALEPKTKADQEKMGL
HHHHHHCHHHHHHCCCCCCCCCCHHCCHHHHHHHHHCCCCHHHHEEECCCCCCCHHHHHH
ALSKLAEEDPTFRTYTDEETGQTIIAGMGELHLDIIVDRMRREFKVETNVGAPQVSYRET
HHHHHHCCCCCEEEECCCCCCCEEECCCCHHHHHHHHHHHHHHEEEECCCCCCCCCHHHH
IKLPAKAEGKYVKQSGGRGSYGHVVIEFEPNVDKGFEVDKITGGRVSKEYINAARVGLEN
EECCCCCCCCHHHCCCCCCCCCEEEEEECCCCCCCCEEECCCCCCCHHHHHHHHHHHHHH
ALTNGVVAGYPMIDVKATIVDGSMHDVDSNEMAYKIAASFALKEACKKMNPVILEPIMNV
HHHCCEEECCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCE
EVTVPDEYYGDVMGNISSKRGLIEGSEQRGNAQTIKSKVPLTEMFGYATELRSFTQGRGN
EEECCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
YTMIFSHYAEAPRSIAEEIIKKSGK
EEEEEHHHHHCHHHHHHHHHHHCCC
>Mature Secondary Structure 
PREFSLENTRNLGIMAHIDAGKTTTTERILFHTGKIHKIGETHEGASQMDMAQEQERGI
CCCCCCCCCCCCEEEEEECCCCCCCHHEEEEECCCEEECCCCCCCHHHHHHHHHHHCCE
TITSAATTAFKNNRFNIIDTPGHVDFTVEVERSLRVLDGAVAVLDGQSGVEPQTETVRQA
EEEECHHHHCCCCCEEEEECCCCEEEEEEHHHHHHHHCCEEEEEECCCCCCCHHHHHHHH
TTYRVPRIVFVNKMDKTGADFIYSVKSIGDRLGAKAAPIQLPIGAEDNFTGIIDLVEMKA
HHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHH
YEFDGKAEEIAKEIEIPADLKDQAEILRSELVEAAVEYDEELMMKFLDGEEITIPELKQA
HCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
IRKGVIGAEFFPVLAGSAFKNKGVKLLLDAVVDYLPSPLDVPSIKGVLPNGEEAERHADD
HHHCCCCHHHHHHHCCCHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCC
NEPFSALAFKVMTDPFVGKLTFFRVYSGILTKGSYVLNSTKGDKERVGRILQMHANNRNE
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCHHH
IEEVYAGDIAAAVGLKNTTTGDTLVDEKHEIILESMVFPEPVIQLALEPKTKADQEKMGL
HHHHHHCHHHHHHCCCCCCCCCCHHCCHHHHHHHHHCCCCHHHHEEECCCCCCCHHHHHH
ALSKLAEEDPTFRTYTDEETGQTIIAGMGELHLDIIVDRMRREFKVETNVGAPQVSYRET
HHHHHHCCCCCEEEECCCCCCCEEECCCCHHHHHHHHHHHHHHEEEECCCCCCCCCHHHH
IKLPAKAEGKYVKQSGGRGSYGHVVIEFEPNVDKGFEVDKITGGRVSKEYINAARVGLEN
EECCCCCCCCHHHCCCCCCCCCEEEEEECCCCCCCCEEECCCCCCCHHHHHHHHHHHHHH
ALTNGVVAGYPMIDVKATIVDGSMHDVDSNEMAYKIAASFALKEACKKMNPVILEPIMNV
HHHCCEEECCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCE
EVTVPDEYYGDVMGNISSKRGLIEGSEQRGNAQTIKSKVPLTEMFGYATELRSFTQGRGN
EEECCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
YTMIFSHYAEAPRSIAEEIIKKSGK
EEEEEHHHHHCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA