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The map label for this gene is tuf [H]

Identifier: 50365437

GI number: 50365437

Start: 727922

End: 729106

Strand: Reverse

Name: tuf [H]

Synonym: Mfl621

Alternate gene names: 50365437

Gene position: 729106-727922 (Counterclockwise)

Preceding gene: 50365438

Following gene: 50365436

Centisome position: 91.92

GC content: 37.81

Gene sequence:

>1185_bases
ATGGCAAAAGAAGCTTTTGACCGTAGTTTACCTCACGTTAACATTGGTACTATTGGACACGTTGACCACGGTAAAACTAC
ATTAACTGCTGCTATTACTAAAGTTTTAGCAGATAAAGGTGGAGCAGAATTTAAAGATTACGCAAATATCGATAACGCTC
CAGAAGAAAGAGAACGTGGAATTACAATTAATACTTCACACGTTGAATATAAAACAGAAAACAGACACTACGCACACGTA
GACTGTCCAGGACACGCCGATTATGTTAAAAACATGATTACAGGTGCTGCTCAAATGGATGGTGGGATCTTAGTTGTTGC
TGCAACTGATGGACCTATGCCTCAAACTCGTGAACACATCTTATTATCAAGACAAGTTGGAGTTCCAAAAATTGTTGTTT
TCTTAAACAAATGTGACATGGTTGATGATGAAGAAATGATCGACTTAGTTGAAATGGAAGTTAGAGACTTATTATCAGCT
TACGACTTCGATGGAGACGGAGCACCAGTTATCCGTGGATCAGCTTTAGGAGCTTTAAACGGAGAAGCTAAATGAGTTGC
TGCTATTGAAGAATTAATGGCTGCAGTTGATGAATACATCCCAACTCCAACTCGTGATTCAGATAAAACATTCTTAATGC
CTGTTGAAGACGTTTTCACAATTACAGGACGTGGAACTGTTGCTACAGGACGTGTTGAACGTGGAACAATTAAAGTTAAC
GAAGAAGTTGAAATCGTTGGATTAGTTGAAGAAGCTAAAAAAACTGTTGTTACTGGATTAGAAATGTTCAGAAAATTATT
AGATTTTGCTGAAGCTGGAGACAACGTTGGAGCATTACTACGTGGTGTTGATAGAGAAAGTATTGAACGTGGACAAGTTT
TAGCTAAACCTGGAACTATTAAACCTCATACTAAATTACAAGCATCAGTTTATGCTTTAACTACTGAAGAAGGTGGACGT
CAAAAACCATTCTTTAACAAATACCGTCCTCAATTCTACTTCCGTACAACTGATGTTACTGGTGAAGTAATCTTACCTGC
AGGAACAGACATGGTTATGCCTGGAGATAACGTTGAAATGACAGTTGAATTAATTAAACCAATTGCCGTTGAAGATGGAA
CAAAATTCTCAATCCGTGAAGGTGGAAGAACTATTGGTGCTGGAACAGTTATTTCAGTTCAATAA

Upstream 100 bases:

>100_bases
TTCTTAATTAATAAGAATAAACAACGCCCCTACGGGGGCGGTAAAGATACTAGAAAGTAAATCTAATATCGCTAGATATA
AATAAAGGAGAAATACAAAA

Downstream 100 bases:

>100_bases
TACCAAAACAAAAAATTGCTTAACGGCAATTTTTTTTGTTATCATTAGAAAAGAAAAGGTGGAAAAATGCAAACAAGAAA
ATCATTATATTTAATAGGAA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MAKEAFDRSLPHVNIGTIGHVDHGKTTLTAAITKVLADKGGAEFKDYANIDNAPEERERGITINTSHVEYKTENRHYAHV
DCPGHADYVKNMITGAAQMDGGILVVAATDGPMPQTREHILLSRQVGVPKIVVFLNKCDMVDDEEMIDLVEMEVRDLLSA
YDFDGDGAPVIRGSALGALNGEAKWVAAIEELMAAVDEYIPTPTRDSDKTFLMPVEDVFTITGRGTVATGRVERGTIKVN
EEVEIVGLVEEAKKTVVTGLEMFRKLLDFAEAGDNVGALLRGVDRESIERGQVLAKPGTIKPHTKLQASVYALTTEEGGR
QKPFFNKYRPQFYFRTTDVTGEVILPAGTDMVMPGDNVEMTVELIKPIAVEDGTKFSIREGGRTIGAGTVISVQ

Sequences:

>Translated_394_residues
MAKEAFDRSLPHVNIGTIGHVDHGKTTLTAAITKVLADKGGAEFKDYANIDNAPEERERGITINTSHVEYKTENRHYAHV
DCPGHADYVKNMITGAAQMDGGILVVAATDGPMPQTREHILLSRQVGVPKIVVFLNKCDMVDDEEMIDLVEMEVRDLLSA
YDFDGDGAPVIRGSALGALNGEAK*VAAIEELMAAVDEYIPTPTRDSDKTFLMPVEDVFTITGRGTVATGRVERGTIKVN
EEVEIVGLVEEAKKTVVTGLEMFRKLLDFAEAGDNVGALLRGVDRESIERGQVLAKPGTIKPHTKLQASVYALTTEEGGR
QKPFFNKYRPQFYFRTTDVTGEVILPAGTDMVMPGDNVEMTVELIKPIAVEDGTKFSIREGGRTIGAGTVISVQ
>Mature_393_residues
AKEAFDRSLPHVNIGTIGHVDHGKTTLTAAITKVLADKGGAEFKDYANIDNAPEERERGITINTSHVEYKTENRHYAHVD
CPGHADYVKNMITGAAQMDGGILVVAATDGPMPQTREHILLSRQVGVPKIVVFLNKCDMVDDEEMIDLVEMEVRDLLSAY
DFDGDGAPVIRGSALGALNGEAK*VAAIEELMAAVDEYIPTPTRDSDKTFLMPVEDVFTITGRGTVATGRVERGTIKVNE
EVEIVGLVEEAKKTVVTGLEMFRKLLDFAEAGDNVGALLRGVDRESIERGQVLAKPGTIKPHTKLQASVYALTTEEGGRQ
KPFFNKYRPQFYFRTTDVTGEVILPAGTDMVMPGDNVEMTVELIKPIAVEDGTKFSIREGGRTIGAGTVISVQ

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=391, Percent_Identity=57.5447570332481, Blast_Score=474, Evalue=1e-134,
Organism=Homo sapiens, GI4503471, Length=326, Percent_Identity=33.1288343558282, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI4503475, Length=445, Percent_Identity=28.314606741573, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI223555963, Length=428, Percent_Identity=29.9065420560748, Blast_Score=145, Evalue=7e-35,
Organism=Homo sapiens, GI5729864, Length=428, Percent_Identity=29.9065420560748, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI54607086, Length=315, Percent_Identity=31.7460317460317, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI46094014, Length=332, Percent_Identity=28.0120481927711, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI194097354, Length=333, Percent_Identity=27.027027027027, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI194018522, Length=333, Percent_Identity=27.027027027027, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI194018520, Length=333, Percent_Identity=27.027027027027, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI4503507, Length=309, Percent_Identity=28.1553398058252, Blast_Score=93, Evalue=4e-19,
Organism=Escherichia coli, GI1790412, Length=393, Percent_Identity=71.7557251908397, Blast_Score=553, Evalue=1e-159,
Organism=Escherichia coli, GI1789737, Length=393, Percent_Identity=71.7557251908397, Blast_Score=552, Evalue=1e-158,
Organism=Escherichia coli, GI2367247, Length=333, Percent_Identity=30.9309309309309, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI48994988, Length=276, Percent_Identity=35.1449275362319, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789108, Length=146, Percent_Identity=33.5616438356164, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1788922, Length=132, Percent_Identity=32.5757575757576, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=398, Percent_Identity=50.5025125628141, Blast_Score=386, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI25141371, Length=380, Percent_Identity=43.421052631579, Blast_Score=323, Evalue=8e-89,
Organism=Caenorhabditis elegans, GI17552884, Length=328, Percent_Identity=33.2317073170732, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17569207, Length=328, Percent_Identity=33.2317073170732, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI115532067, Length=424, Percent_Identity=27.5943396226415, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI115532065, Length=424, Percent_Identity=27.5943396226415, Blast_Score=131, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI32566303, Length=310, Percent_Identity=31.2903225806452, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17506081, Length=272, Percent_Identity=29.7794117647059, Blast_Score=124, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI32566629, Length=323, Percent_Identity=27.2445820433437, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI32566301, Length=162, Percent_Identity=34.5679012345679, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17509919, Length=447, Percent_Identity=25.0559284116331, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17557151, Length=230, Percent_Identity=26.9565217391304, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=395, Percent_Identity=66.3291139240506, Blast_Score=526, Evalue=1e-150,
Organism=Saccharomyces cerevisiae, GI6325337, Length=344, Percent_Identity=31.9767441860465, Blast_Score=143, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6319594, Length=344, Percent_Identity=31.9767441860465, Blast_Score=143, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6322937, Length=363, Percent_Identity=25.8953168044077, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6320863, Length=309, Percent_Identity=27.5080906148867, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6320377, Length=344, Percent_Identity=30.8139534883721, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323320, Length=238, Percent_Identity=30.2521008403361, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6323098, Length=135, Percent_Identity=34.8148148148148, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281363316, Length=392, Percent_Identity=57.6530612244898, Blast_Score=466, Evalue=1e-131,
Organism=Drosophila melanogaster, GI17864358, Length=392, Percent_Identity=57.6530612244898, Blast_Score=466, Evalue=1e-131,
Organism=Drosophila melanogaster, GI19921738, Length=385, Percent_Identity=50.1298701298701, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI45553807, Length=328, Percent_Identity=32.6219512195122, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI45553816, Length=328, Percent_Identity=32.6219512195122, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24651721, Length=328, Percent_Identity=32.6219512195122, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI17864154, Length=328, Percent_Identity=32.6219512195122, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24652838, Length=326, Percent_Identity=31.9018404907975, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI17137572, Length=326, Percent_Identity=31.9018404907975, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI19922690, Length=303, Percent_Identity=31.3531353135314, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI45550900, Length=430, Percent_Identity=28.6046511627907, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI17137380, Length=320, Percent_Identity=28.75, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24647046, Length=309, Percent_Identity=27.831715210356, Blast_Score=95, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24647048, Length=309, Percent_Identity=27.831715210356, Blast_Score=95, Evalue=8e-20,
Organism=Drosophila melanogaster, GI78706572, Length=240, Percent_Identity=29.5833333333333, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI28572034, Length=228, Percent_Identity=32.8947368421053, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 42701; Mature: 42570

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEAFDRSLPHVNIGTIGHVDHGKTTLTAAITKVLADKGGAEFKDYANIDNAPEERERG
CCCHHHHHCCCEEECCEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHCCCCCCCHHHHCC
ITINTSHVEYKTENRHYAHVDCPGHADYVKNMITGAAQMDGGILVVAATDGPMPQTREHI
EEEECCEEEEEECCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHE
LLSRQVGVPKIVVFLNKCDMVDDEEMIDLVEMEVRDLLSAYDFDGDGAPVIRGSALGALN
EEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCC
GEAKVAAIEELMAAVDEYIPTPTRDSDKTFLMPVEDVFTITGRGTVATGRVERGTIKVNE
CCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHEEEEECCCEEEECCEECCEEEECC
EVEIVGLVEEAKKTVVTGLEMFRKLLDFAEAGDNVGALLRGVDRESIERGQVLAKPGTIK
CEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHCCCEEECCCCCC
PHTKLQASVYALTTEEGGRQKPFFNKYRPQFYFRTTDVTGEVILPAGTDMVMPGDNVEMT
CCCCEEEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCEEEEECCCCEEECCCCCEEE
VELIKPIAVEDGTKFSIREGGRTIGAGTVISVQ
EEECCCEEECCCCEEEECCCCEEECCCEEEEEC
>Mature Secondary Structure 
AKEAFDRSLPHVNIGTIGHVDHGKTTLTAAITKVLADKGGAEFKDYANIDNAPEERERG
CCHHHHHCCCEEECCEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHCCCCCCCHHHHCC
ITINTSHVEYKTENRHYAHVDCPGHADYVKNMITGAAQMDGGILVVAATDGPMPQTREHI
EEEECCEEEEEECCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHE
LLSRQVGVPKIVVFLNKCDMVDDEEMIDLVEMEVRDLLSAYDFDGDGAPVIRGSALGALN
EEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCC
GEAKVAAIEELMAAVDEYIPTPTRDSDKTFLMPVEDVFTITGRGTVATGRVERGTIKVNE
CCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHEEEEECCCEEEECCEECCEEEECC
EVEIVGLVEEAKKTVVTGLEMFRKLLDFAEAGDNVGALLRGVDRESIERGQVLAKPGTIK
CEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHCCCEEECCCCCC
PHTKLQASVYALTTEEGGRQKPFFNKYRPQFYFRTTDVTGEVILPAGTDMVMPGDNVEMT
CCCCEEEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCEEEEECCCCEEECCCCCEEE
VELIKPIAVEDGTKFSIREGGRTIGAGTVISVQ
EEECCCEEECCCCEEEECCCCEEECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA