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The map label for this gene is uvrD [H]

Identifier: 50365383

GI number: 50365383

Start: 657457

End: 659628

Strand: Reverse

Name: uvrD [H]

Synonym: Mfl566

Alternate gene names: 50365383

Gene position: 659628-657457 (Counterclockwise)

Preceding gene: 50365384

Following gene: 50365382

Centisome position: 83.16

GC content: 26.98

Gene sequence:

>2172_bases
ATGAATAATTTATTATTACAATTAAATGAACAACAATTGGCAGCGGTGACAATAACAGACAAACCGTTAAGAATAGTTGC
TGGTGCTGGTTCTGGTAAAACTAAAGTAATTACCACAAAAATTGCTTATTTAATTGAAGAACTAAAAATGGCTCCTTATA
AAATATTAGCTGTGACTTTTACCAATAAAGCAGCAAGAGAGATGAGAGATAGAGTAACAAAAATTATACCTGACTTAAAA
AGTAATCCTCATATTTCTACATTTCATGCTTGATGTTCAAGAGTTTTAAGAGAAGATTTTGAATTAGTAGGTTTAAATAA
AAACTTTTTAATTATCGATCAAGGTGATCAAATTGCAATTTTAAGAAGTTTAATAAATGAACACTTTAGTCATTTACCTG
AATTAAAAAAATACACAGAGAAAAAAATTATTTATAGAATTGGTAATTGAAAGAATGATTTAACATCTCCATTAGAAGCA
ACAGAAGAATGTTATAGTGGGATTGAAAGAGCTATTGCTACGTTATATCAAAAATATGAAGATTATTTAAAAACAATAAA
TTCAATTGATTTTAATGATTTACAAGTTCTTGTTTTTAAACTTTTTAATGAGCATCCTGAAGCTTTAGAAAAATGAAGAA
ATAGATATGATTATGTTATGGTTGATGAGTTTCAAGACACCAACGATCTACAGTTTGATTTAATAAAATTTTTAACAAGA
GATAAAAATAATTTAACAGTAGTTGGAGACCCAGATCAAACAATATATTCTTGACGTGGGGCAAAAGTTGATATTATTTT
GAATTTTACAAAATCATTTTCAAACGGTGTTAGCATCACTCTTAATCAAAATTATAGATCAACTCAGAAAATTTTAGACT
TAGCAAATGACTTTATTAATAATAATAAAAATAGAGAAGCTAAAGATATTTTTACAAATAATGCAACAGGAGATTTACCA
ATAATTAAAGAATGTGAATCTAGAAATGATGAAGCAAGATATGTAACTTTAAAAATTAAAGAATTAATCAAAGATGGTTA
TGAGTATAAAGACATTTTTGTACTATACAGGCTTAATGCTTGATCACAAGAATTTGAAAAAGAATTTCAAAATAATAAAA
TTCCTTTTCAATTAATTGGTGGAATTCGTTTTAAAGACAGAAAAGTAATTAAAGAAGCAAATGCTTTTTTAAGAACTTTA
GCTACACAAGATGAACAAGCTGTTGAAAGAGTTTTAAAAAGCATTCCAAAAGTTGGAGATGTAACAATTAAGAAATTAAA
AGAAAAAGCCAATGACATGAATGTATCGCTTTATGACTTAATTGTTAATGAAGATGAATTGCATATAAATCAAGTTTCAA
AACATTTGACAACAATAAGAAATGTTCTTTCAAACTCTGTAAATGTTTATAATGAAAGCAAGAATATCAGAATGACATTG
CAATATATGCTTGTTGAGTCAGGATATATTCAAAAATTGAAGGATACAGAAAAAAAAGAAGATTTAAGTTACATAGATTC
ATTGTATGATCAACTAGAAAATTTTGATAAATCATATCAAACTGAAGAATTAAACGAAGGATCAACAGATAAAATAGTTT
CTTATTTACAAGAAGAAGCACTATTAAATTCTGATGCTGATGATATTGAAGCACAAAAAGTTACTTTATTAACTGTACAT
GCTGCAAAAGGTTTAGAAAATAAAATAGTTTTTGTTGTCGGGCTTAATCAAGATGTATTTCCAGGTAGATTATCTTCAAA
TTCAGCTAGAGAAATAGAAGAAGAAAGAAGAACTTTATATGTTGCTATAACAAGAGCAGAAGAAAAAGTTTATATAACGT
ATATTAAAGGTGAATTTTCATTTATTTCACAATCAGAATTAGCACCATCAAAATTTATTAAGGAATTCGACCAAGATTTA
TATCAGTTTGAAGGTAAATATCAAAAGAGTTCAAATCCGTTTGCCAATACAAGTCACTTAAGCTCAATCAAATCAAATCA
GCATGTGAAAACATCTGCAGGATATGAGAAGAATGATGTTATAGAACACATGATTTTTGGTGATGGAGTTGTTATTGAAA
TAAACGACCAAACAATGAAGGTTGCTTTTAGTTCAAGTTATGGAATTATGCCAATTTCAGTTAATGACGCAACCATATCA
AAGAAAAATTAA

Upstream 100 bases:

>100_bases
TATAATTCAATACAAGTTGATAAACCTAGCTTAACTGAAAATTTAAAAATTGAAAAAAAGAATCAAAAACAAATAGAACA
AAAAGAAAAAGGAGAAAGCC

Downstream 100 bases:

>100_bases
TTAATAAGAATTGATAATGATATCAACTTATTGATATAATAATTAAGTATCAAAAAATACAGGAGGAAAATTAAATGGCA
CAATTTACAGCAGTTATAAC

Product: repair endonuclease ATP-dependent DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 723; Mature: 723

Protein sequence:

>723_residues
MNNLLLQLNEQQLAAVTITDKPLRIVAGAGSGKTKVITTKIAYLIEELKMAPYKILAVTFTNKAAREMRDRVTKIIPDLK
SNPHISTFHAWCSRVLREDFELVGLNKNFLIIDQGDQIAILRSLINEHFSHLPELKKYTEKKIIYRIGNWKNDLTSPLEA
TEECYSGIERAIATLYQKYEDYLKTINSIDFNDLQVLVFKLFNEHPEALEKWRNRYDYVMVDEFQDTNDLQFDLIKFLTR
DKNNLTVVGDPDQTIYSWRGAKVDIILNFTKSFSNGVSITLNQNYRSTQKILDLANDFINNNKNREAKDIFTNNATGDLP
IIKECESRNDEARYVTLKIKELIKDGYEYKDIFVLYRLNAWSQEFEKEFQNNKIPFQLIGGIRFKDRKVIKEANAFLRTL
ATQDEQAVERVLKSIPKVGDVTIKKLKEKANDMNVSLYDLIVNEDELHINQVSKHLTTIRNVLSNSVNVYNESKNIRMTL
QYMLVESGYIQKLKDTEKKEDLSYIDSLYDQLENFDKSYQTEELNEGSTDKIVSYLQEEALLNSDADDIEAQKVTLLTVH
AAKGLENKIVFVVGLNQDVFPGRLSSNSAREIEEERRTLYVAITRAEEKVYITYIKGEFSFISQSELAPSKFIKEFDQDL
YQFEGKYQKSSNPFANTSHLSSIKSNQHVKTSAGYEKNDVIEHMIFGDGVVIEINDQTMKVAFSSSYGIMPISVNDATIS
KKN

Sequences:

>Translated_723_residues
MNNLLLQLNEQQLAAVTITDKPLRIVAGAGSGKTKVITTKIAYLIEELKMAPYKILAVTFTNKAAREMRDRVTKIIPDLK
SNPHISTFHA*CSRVLREDFELVGLNKNFLIIDQGDQIAILRSLINEHFSHLPELKKYTEKKIIYRIGN*KNDLTSPLEA
TEECYSGIERAIATLYQKYEDYLKTINSIDFNDLQVLVFKLFNEHPEALEK*RNRYDYVMVDEFQDTNDLQFDLIKFLTR
DKNNLTVVGDPDQTIYS*RGAKVDIILNFTKSFSNGVSITLNQNYRSTQKILDLANDFINNNKNREAKDIFTNNATGDLP
IIKECESRNDEARYVTLKIKELIKDGYEYKDIFVLYRLNA*SQEFEKEFQNNKIPFQLIGGIRFKDRKVIKEANAFLRTL
ATQDEQAVERVLKSIPKVGDVTIKKLKEKANDMNVSLYDLIVNEDELHINQVSKHLTTIRNVLSNSVNVYNESKNIRMTL
QYMLVESGYIQKLKDTEKKEDLSYIDSLYDQLENFDKSYQTEELNEGSTDKIVSYLQEEALLNSDADDIEAQKVTLLTVH
AAKGLENKIVFVVGLNQDVFPGRLSSNSAREIEEERRTLYVAITRAEEKVYITYIKGEFSFISQSELAPSKFIKEFDQDL
YQFEGKYQKSSNPFANTSHLSSIKSNQHVKTSAGYEKNDVIEHMIFGDGVVIEINDQTMKVAFSSSYGIMPISVNDATIS
KKN
>Mature_723_residues
MNNLLLQLNEQQLAAVTITDKPLRIVAGAGSGKTKVITTKIAYLIEELKMAPYKILAVTFTNKAAREMRDRVTKIIPDLK
SNPHISTFHA*CSRVLREDFELVGLNKNFLIIDQGDQIAILRSLINEHFSHLPELKKYTEKKIIYRIGN*KNDLTSPLEA
TEECYSGIERAIATLYQKYEDYLKTINSIDFNDLQVLVFKLFNEHPEALEK*RNRYDYVMVDEFQDTNDLQFDLIKFLTR
DKNNLTVVGDPDQTIYS*RGAKVDIILNFTKSFSNGVSITLNQNYRSTQKILDLANDFINNNKNREAKDIFTNNATGDLP
IIKECESRNDEARYVTLKIKELIKDGYEYKDIFVLYRLNA*SQEFEKEFQNNKIPFQLIGGIRFKDRKVIKEANAFLRTL
ATQDEQAVERVLKSIPKVGDVTIKKLKEKANDMNVSLYDLIVNEDELHINQVSKHLTTIRNVLSNSVNVYNESKNIRMTL
QYMLVESGYIQKLKDTEKKEDLSYIDSLYDQLENFDKSYQTEELNEGSTDKIVSYLQEEALLNSDADDIEAQKVTLLTVH
AAKGLENKIVFVVGLNQDVFPGRLSSNSAREIEEERRTLYVAITRAEEKVYITYIKGEFSFISQSELAPSKFIKEFDQDL
YQFEGKYQKSSNPFANTSHLSSIKSNQHVKTSAGYEKNDVIEHMIFGDGVVIEINDQTMKVAFSSSYGIMPISVNDATIS
KKN

Specific function: Has both ATPase and helicase activities. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. Involved in the post-incision events of nucleotide exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=718, Percent_Identity=30.2228412256267, Blast_Score=320, Evalue=2e-88,
Organism=Escherichia coli, GI48994965, Length=700, Percent_Identity=32.5714285714286, Blast_Score=278, Evalue=1e-75,
Organism=Saccharomyces cerevisiae, GI6322369, Length=744, Percent_Identity=26.4784946236559, Blast_Score=154, Evalue=5e-38,
Organism=Saccharomyces cerevisiae, GI6324477, Length=670, Percent_Identity=21.7910447761194, Blast_Score=84, Evalue=8e-17,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 82272; Mature: 82272

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNLLLQLNEQQLAAVTITDKPLRIVAGAGSGKTKVITTKIAYLIEELKMAPYKILAVTF
CCCEEEEECCCEEEEEEECCCCEEEEEECCCCCCEEEHHHHHHHHHHHCCCCEEEEEEEE
TNKAAREMRDRVTKIIPDLKSNPHISTFHACSRVLREDFELVGLNKNFLIIDQGDQIAIL
CCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCEEEEECCCCHHHH
RSLINEHFSHLPELKKYTEKKIIYRIGNKNDLTSPLEATEECYSGIERAIATLYQKYEDY
HHHHHHHHHHCHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LKTINSIDFNDLQVLVFKLFNEHPEALEKRNRYDYVMVDEFQDTNDLQFDLIKFLTRDKN
HHHHHCCCCCHHHHHHHHHHCCCHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHCCCC
NLTVVGDPDQTIYSRGAKVDIILNFTKSFSNGVSITLNQNYRSTQKILDLANDFINNNKN
CEEEECCCCHHHHHCCCEEEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCC
REAKDIFTNNATGDLPIIKECESRNDEARYVTLKIKELIKDGYEYKDIFVLYRLNASQEF
CCHHHHHCCCCCCCCCHHHHHCCCCCCCEEEEEEHHHHHHCCCCEEEEEEEEEECCCHHH
EKEFQNNKIPFQLIGGIRFKDRKVIKEANAFLRTLATQDEQAVERVLKSIPKVGDVTIKK
HHHHCCCCCCEEEECCCEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHH
LKEKANDMNVSLYDLIVNEDELHINQVSKHLTTIRNVLSNSVNVYNESKNIRMTLQYMLV
HHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEEEHHH
ESGYIQKLKDTEKKEDLSYIDSLYDQLENFDKSYQTEELNEGSTDKIVSYLQEEALLNSD
CCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCHHHHHHHHHHHHHHCCC
ADDIEAQKVTLLTVHAAKGLENKIVFVVGLNQDVFPGRLSSNSAREIEEERRTLYVAITR
CCCCCCCEEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHCCCEEEEEEEE
AEEKVYITYIKGEFSFISQSELAPSKFIKEFDQDLYQFEGKYQKSSNPFANTSHLSSIKS
CCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCC
NQHVKTSAGYEKNDVIEHMIFGDGVVIEINDQTMKVAFSSSYGIMPISVNDATISKKN
CCCEECCCCCCHHHHHHHHEECCCEEEEECCCEEEEEEECCCCEEEEEECCCCCCCCC
>Mature Secondary Structure
MNNLLLQLNEQQLAAVTITDKPLRIVAGAGSGKTKVITTKIAYLIEELKMAPYKILAVTF
CCCEEEEECCCEEEEEEECCCCEEEEEECCCCCCEEEHHHHHHHHHHHCCCCEEEEEEEE
TNKAAREMRDRVTKIIPDLKSNPHISTFHACSRVLREDFELVGLNKNFLIIDQGDQIAIL
CCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCEEEEECCCCHHHH
RSLINEHFSHLPELKKYTEKKIIYRIGNKNDLTSPLEATEECYSGIERAIATLYQKYEDY
HHHHHHHHHHCHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LKTINSIDFNDLQVLVFKLFNEHPEALEKRNRYDYVMVDEFQDTNDLQFDLIKFLTRDKN
HHHHHCCCCCHHHHHHHHHHCCCHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHCCCC
NLTVVGDPDQTIYSRGAKVDIILNFTKSFSNGVSITLNQNYRSTQKILDLANDFINNNKN
CEEEECCCCHHHHHCCCEEEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCC
REAKDIFTNNATGDLPIIKECESRNDEARYVTLKIKELIKDGYEYKDIFVLYRLNASQEF
CCHHHHHCCCCCCCCCHHHHHCCCCCCCEEEEEEHHHHHHCCCCEEEEEEEEEECCCHHH
EKEFQNNKIPFQLIGGIRFKDRKVIKEANAFLRTLATQDEQAVERVLKSIPKVGDVTIKK
HHHHCCCCCCEEEECCCEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHH
LKEKANDMNVSLYDLIVNEDELHINQVSKHLTTIRNVLSNSVNVYNESKNIRMTLQYMLV
HHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEEEHHH
ESGYIQKLKDTEKKEDLSYIDSLYDQLENFDKSYQTEELNEGSTDKIVSYLQEEALLNSD
CCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCHHHHHHHHHHHHHHCCC
ADDIEAQKVTLLTVHAAKGLENKIVFVVGLNQDVFPGRLSSNSAREIEEERRTLYVAITR
CCCCCCCEEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHCCCEEEEEEEE
AEEKVYITYIKGEFSFISQSELAPSKFIKEFDQDLYQFEGKYQKSSNPFANTSHLSSIKS
CCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCC
NQHVKTSAGYEKNDVIEHMIFGDGVVIEINDQTMKVAFSSSYGIMPISVNDATISKKN
CCCEECCCCCCHHHHHHHHEECCCEEEEECCCEEEEEEECCCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8253782 [H]