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The map label for this gene is ptsH [H]

Identifier: 50365382

GI number: 50365382

Start: 657113

End: 657382

Strand: Reverse

Name: ptsH [H]

Synonym: Mfl565

Alternate gene names: 50365382

Gene position: 657382-657113 (Counterclockwise)

Preceding gene: 50365383

Following gene: 50365381

Centisome position: 82.87

GC content: 33.7

Gene sequence:

>270_bases
ATGGCACAATTTACAGCAGTTATAACTGACAAAATTGGATTACATGCAAGACCAGCTACTGCAATTATTTCAGCAGCTTC
AAAATATGGTTCAGACATTCAAATCGTTTCAGGAAGTAAATCAGGAAACTTAAGATCAATAATGAACATCTTATCAATGC
AAATTAAAAATGGTGATGAAATTACTATTATTGCTGATGGTAGTGATGCTGAAGAAGCAGTTGCTGGTATTAAAGAAGCA
ATGATCGCTGCAGCATTAATTGAAGGTTAA

Upstream 100 bases:

>100_bases
ACGCAACCATATCAAAGAAAAATTAATTAATAAGAATTGATAATGATATCAACTTATTGATATAATAATTAAGTATCAAA
AAATACAGGAGGAAAATTAA

Downstream 100 bases:

>100_bases
AACAAAATTAATGAAAATTGCTTATAGCAATTTTTTTATATGTTTTAATTAATTAGACATGAAAAAAGGAGAAACATGAA
ATTAATAATTAATAAACTCA

Product: phosphotransferase system phosphohistidine-containing protein

Products: fructose-6-phosphate [Cytoplasm]; pyruvate; fructose-1-phosphate [Cytoplasm] [C]

Alternate protein names: Histidine-containing protein [H]

Number of amino acids: Translated: 89; Mature: 88

Protein sequence:

>89_residues
MAQFTAVITDKIGLHARPATAIISAASKYGSDIQIVSGSKSGNLRSIMNILSMQIKNGDEITIIADGSDAEEAVAGIKEA
MIAAALIEG

Sequences:

>Translated_89_residues
MAQFTAVITDKIGLHARPATAIISAASKYGSDIQIVSGSKSGNLRSIMNILSMQIKNGDEITIIADGSDAEEAVAGIKEA
MIAAALIEG
>Mature_88_residues
AQFTAVITDKIGLHARPATAIISAASKYGSDIQIVSGSKSGNLRSIMNILSMQIKNGDEITIIADGSDAEEAVAGIKEAM
IAAALIEG

Specific function: P-Ser-HPr interacts with the catabolite control protein A (CcpA), forming a complex that binds to DNA at the catabolite response elements cre, operator sites preceding a large number of catabolite-regulated genes. Thus, P-Ser-HPr is a corepressor in carbo

COG id: COG1925

COG function: function code G; Phosphotransferase system, HPr-related proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HPr domain [H]

Homologues:

Organism=Escherichia coli, GI1788494, Length=91, Percent_Identity=37.3626373626374, Blast_Score=60, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114 [H]

Pfam domain/function: PF00381 PTS-HPr [H]

EC number: 2.7.1.69 [C]

Molecular weight: Translated: 9147; Mature: 9015

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00369 PTS_HPR_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQFTAVITDKIGLHARPATAIISAASKYGSDIQIVSGSKSGNLRSIMNILSMQIKNGDE
CCCEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCE
ITIIADGSDAEEAVAGIKEAMIAAALIEG
EEEEECCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AQFTAVITDKIGLHARPATAIISAASKYGSDIQIVSGSKSGNLRSIMNILSMQIKNGDE
CCEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCE
ITIIADGSDAEEAVAGIKEAMIAAALIEG
EEEEECCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Fructose [Periplasm]; phosphoenolpyruvate [C]

Specific reaction: phosphoenolpyruvate + fructose [Periplasm] = fructose-6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + fructose [Periplasm] = fructose-1-phosphate [Cytoplasm] + pyruvate [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253782; 8710835; 7582895 [H]