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The map label for this gene is folC [H]

Identifier: 50364875

GI number: 50364875

Start: 78343

End: 79449

Strand: Direct

Name: folC [H]

Synonym: Mfl060

Alternate gene names: 50364875

Gene position: 78343-79449 (Clockwise)

Preceding gene: 50364874

Following gene: 50364876

Centisome position: 9.88

GC content: 24.3

Gene sequence:

>1107_bases
ATGATTTCAGTAGATAAGTTTTTAATACCTATTCAACAAAGATTTCAAAATGAATACAATTTAGATAAAGTTTTAAAACA
ATTAAATAATATTGAAAAGAAGATACCAACTATTAATGTGGTTGGGACAAATGGAAAAGGAAGCACTTCTTTTTTCTTGT
CTCATGGGCTAAAAACAAAATATAAAAAAGTTGGATTATTTATTTCTCCAGCTTTCTTATATCACAATGAGAGAATTCAA
ATTAATAACGAACCAATAAGTGATGAAGATCTAAAAAAATATATTTCAAAAGTTGAAAAATTTATTAAAGATTACAATCT
AACATTTTTTGAAATATGAACTTTAATAGCAATCATGTATTTTTATGATAACAAAGTTGATATTGCTGTAATTGAAGCAG
GTATAGGTGGTTTAAAGGACTCAACAAATTTATTTTCAAATCAGATAGCAGCAATTTTAACATCTGTTTCATTTGATCAC
ACAGAAATATTAGGTAATAAAATTGAAGAAATAATTGATCAAAAAGTTGGGATAGTAAAAGAAAATTCAACTTTATATGT
TAGTGCAGATAATAAAGAGCATTTTGAAACTATTAAAAATGCAATTAAGGTTAATGCAAGCATTATATATTCTGATAAAT
ATGAAAAAGAAAAAATTGAATACCAAAAAGGCAACAAAGGTGTTGTTAAAAATATACTTCAAGATTTAGGGATAAAAGAT
TTATCATGTTTAAATTTAAATTCGTTAACTGGTAGATTTACAGAAATAGAATTTAAAGAGAAAAAAATAATAATAGATGG
TGCTCACAATGTAGACGGAGTTAGAGCACTTATTTCAAGTGTCAGCAATAAAAAAGATTGAACAGTTTTATATGGTGCAA
TTGATGGTAAAGAACATCAAAAAATATTAACAATGTTAGATGAGAATTTTGAAAATGTAAACATAACAACATTTGATTTT
CATAAGGCCTGAGATATTGATTTAGTTAACCATATAAATAAAGTTAAAAATTGAAAAGAATTCATAGATTCTTCTGAAAA
GAATATTATAATTTGTGGTAGTTTGTATTTTATACCGTTGGTTTATAAATATTTGTTAGAAAAATAG

Upstream 100 bases:

>100_bases
GGAATTGGCGGTGGATTAATTGTTGCTGCTGGAACACCAGAACAAGTTGCTGATACAAAAGGTTCATATACGGGAACTTA
TTTAAAGGAATATTTATATA

Downstream 100 bases:

>100_bases
AAAGAAGGATTAATCATGTTATTTTGAATTACTAATATAATTGCTATGTTACTTATTATTGCTATGTTTTTTATTGGTAT
TTTTATTGATAAAATACATG

Product: putative folyl-polyglutamate synthetase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MISVDKFLIPIQQRFQNEYNLDKVLKQLNNIEKKIPTINVVGTNGKGSTSFFLSHGLKTKYKKVGLFISPAFLYHNERIQ
INNEPISDEDLKKYISKVEKFIKDYNLTFFEIWTLIAIMYFYDNKVDIAVIEAGIGGLKDSTNLFSNQIAAILTSVSFDH
TEILGNKIEEIIDQKVGIVKENSTLYVSADNKEHFETIKNAIKVNASIIYSDKYEKEKIEYQKGNKGVVKNILQDLGIKD
LSCLNLNSLTGRFTEIEFKEKKIIIDGAHNVDGVRALISSVSNKKDWTVLYGAIDGKEHQKILTMLDENFENVNITTFDF
HKAWDIDLVNHINKVKNWKEFIDSSEKNIIICGSLYFIPLVYKYLLEK

Sequences:

>Translated_368_residues
MISVDKFLIPIQQRFQNEYNLDKVLKQLNNIEKKIPTINVVGTNGKGSTSFFLSHGLKTKYKKVGLFISPAFLYHNERIQ
INNEPISDEDLKKYISKVEKFIKDYNLTFFEI*TLIAIMYFYDNKVDIAVIEAGIGGLKDSTNLFSNQIAAILTSVSFDH
TEILGNKIEEIIDQKVGIVKENSTLYVSADNKEHFETIKNAIKVNASIIYSDKYEKEKIEYQKGNKGVVKNILQDLGIKD
LSCLNLNSLTGRFTEIEFKEKKIIIDGAHNVDGVRALISSVSNKKD*TVLYGAIDGKEHQKILTMLDENFENVNITTFDF
HKA*DIDLVNHINKVKN*KEFIDSSEKNIIICGSLYFIPLVYKYLLEK
>Mature_368_residues
MISVDKFLIPIQQRFQNEYNLDKVLKQLNNIEKKIPTINVVGTNGKGSTSFFLSHGLKTKYKKVGLFISPAFLYHNERIQ
INNEPISDEDLKKYISKVEKFIKDYNLTFFEI*TLIAIMYFYDNKVDIAVIEAGIGGLKDSTNLFSNQIAAILTSVSFDH
TEILGNKIEEIIDQKVGIVKENSTLYVSADNKEHFETIKNAIKVNASIIYSDKYEKEKIEYQKGNKGVVKNILQDLGIKD
LSCLNLNSLTGRFTEIEFKEKKIIIDGAHNVDGVRALISSVSNKKD*TVLYGAIDGKEHQKILTMLDENFENVNITTFDF
HKA*DIDLVNHINKVKN*KEFIDSSEKNIIICGSLYFIPLVYKYLLEK

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=152, Percent_Identity=39.4736842105263, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI66932990, Length=152, Percent_Identity=39.4736842105263, Blast_Score=104, Evalue=1e-22,
Organism=Escherichia coli, GI1788654, Length=152, Percent_Identity=32.8947368421053, Blast_Score=92, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17553148, Length=165, Percent_Identity=33.3333333333333, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI71984923, Length=165, Percent_Identity=33.3333333333333, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17553150, Length=165, Percent_Identity=33.3333333333333, Blast_Score=94, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6323760, Length=405, Percent_Identity=26.6666666666667, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6324815, Length=171, Percent_Identity=35.0877192982456, Blast_Score=89, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24641571, Length=179, Percent_Identity=32.9608938547486, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24581568, Length=178, Percent_Identity=29.2134831460674, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 41443; Mature: 41443

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISVDKFLIPIQQRFQNEYNLDKVLKQLNNIEKKIPTINVVGTNGKGSTSFFLSHGLKTK
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHCCCHHH
YKKVGLFISPAFLYHNERIQINNEPISDEDLKKYISKVEKFIKDYNLTFFEITLIAIMYF
HHHCCEEEEEHEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
YDNKVDIAVIEAGIGGLKDSTNLFSNQIAAILTSVSFDHTEILGNKIEEIIDQKVGIVKE
HCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCEEEC
NSTLYVSADNKEHFETIKNAIKVNASIIYSDKYEKEKIEYQKGNKGVVKNILQDLGIKDL
CCEEEEECCCHHHHHHHHHHHEECEEEEECCCCCHHHHHHCCCCCHHHHHHHHHCCCCCE
SCLNLNSLTGRFTEIEFKEKKIIIDGAHNVDGVRALISSVSNKKDTVLYGAIDGKEHQKI
EEECCCCCCCCEEEEEEECCEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHH
LTMLDENFENVNITTFDFHKADIDLVNHINKVKNKEFIDSSEKNIIICGSLYFIPLVYKY
HHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHCCCHHCCCCCCCEEEECHHHHHHHHHHH
LLEK
HHCC
>Mature Secondary Structure
MISVDKFLIPIQQRFQNEYNLDKVLKQLNNIEKKIPTINVVGTNGKGSTSFFLSHGLKTK
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHCCCHHH
YKKVGLFISPAFLYHNERIQINNEPISDEDLKKYISKVEKFIKDYNLTFFEITLIAIMYF
HHHCCEEEEEHEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
YDNKVDIAVIEAGIGGLKDSTNLFSNQIAAILTSVSFDHTEILGNKIEEIIDQKVGIVKE
HCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCEEEC
NSTLYVSADNKEHFETIKNAIKVNASIIYSDKYEKEKIEYQKGNKGVVKNILQDLGIKDL
CCEEEEECCCHHHHHHHHHHHEECEEEEECCCCCHHHHHHCCCCCHHHHHHHHHCCCCCE
SCLNLNSLTGRFTEIEFKEKKIIIDGAHNVDGVRALISSVSNKKDTVLYGAIDGKEHQKI
EEECCCCCCCCEEEEEEECCEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHH
LTMLDENFENVNITTFDFHKADIDLVNHINKVKNKEFIDSSEKNIIICGSLYFIPLVYKY
HHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHCCCHHCCCCCCCEEEECHHHHHHHHHHH
LLEK
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]