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The map label for this gene is metG [H]

Identifier: 50364851

GI number: 50364851

Start: 49572

End: 51101

Strand: Direct

Name: metG [H]

Synonym: Mfl036

Alternate gene names: 50364851

Gene position: 49572-51101 (Clockwise)

Preceding gene: 50364849

Following gene: 50364852

Centisome position: 6.25

GC content: 26.21

Gene sequence:

>1530_bases
ATGCCTAAGAAATTTTACGTAACAACACCAATCTATTACCCAAGTGGTAACTTACATATAGGACATGCATACACAACAAC
TTTAGCTGATATTCTTAAAAGATATAAAGACATGCAAGGTTATGAAACATTCTTCTTAACTGGTAGTGATGAACACGGTC
AAAAAATAGAAGAAAAAGCAACAGAAGCTGGCTTAACACCAATTGATTATCTTGAAGATAAAGTTGAAAGTTTTAAAACA
CTTTGAAAAGCTTTAAAAATTGACTACACAAAATTTATAAGAACTACAGATTCATATCATGAAGAAACTGTTAAAAAAAT
ATTTACTTTATTATTAGATAAAGGTTTTATTTACAAAGGCAATTATGAAGGATTATATTGTATTAGTTGTGAAGAATTTT
TAAATGCTGATCAAATAGACGAAAACAATATGTGTAAAATATCTAATACAAAATTAGAATTAGTTAAAGAAGAAACTTAC
TTTTTAAAAACTTCTTTATTTCAAGAGTTCATGGAAAATGAAGTTTTAGCAAGTGAATTTTTAATTCCAGCATATAGAAG
AAATGAAATGTTAAATTCTTTTGTTAAACCAGAGCTTAAAGACTTATCAGTAACAAGAACTTCTTTTTCATGAGGTATAC
CTATTAATGAAGATCCAAAGCATGTTGTTTATGTTTGATTAGATGCTTTAACAAACTATATAACAGCTCTTGGTTATTTA
CAAAATGATGATTCAAATTTTCAAAAATTTTGAGCTGATGAAAATACAGAAATTTTACAATTAGCAGGTAAAGAAATTAT
TCGTTTCCATTCAATATATTGACCTATAATTTTAAAATCACTAGGCTTACGCCAACCAACACACTTATTGGGTCATGGTT
GAATTTTAAGCAAAGACACAAAGATGAGTAAGTCTCTAGGAAACGTTATTGATCCAATAGAGATGATTAACAAATATGGA
GCAGATGCTTTAAGATTTTATATTGGTTATGAGTTACCAACAGAAAAAGATGGTAATTTTACTGATGATTTATTTATTGA
ATCTTTTAATGCACACTTAGCTAATAATGTTGGTAACTTAATATCAAGAATAAATCAAATGATAACTAAATATTTTGATG
GTTTTATTGAAAACGACAATATTGTGTATGATGACAACTTAACAGCGAAAGGTATTGAAACAATTAATTTATATATACAA
AATATGGATCAATACAAAATCAGTGATGCAATAAGAAATATATTAGACTTAGGTAATGCATGTAATAAATACATCGAAGA
AACAATGCCTTGAAATTTAGCTAAAGAAGAAAAAATAGATGAACTAAAAAATGTAATGGCAACATTGCAACGTAATATAT
CTATTATGGTTTTCTTGTTAAAACCAATACTTGTAGAAAACTATGAAGATATGATTGAGCAAATTGGTTTAAGTGGTGTT
GAATTAACGTTTGATAAAATAAACAATTGAGATAAAATAATATTTAAAAAATTAGGAGATAAAAAAATTATCTTCAAAAG
AGTTAATTAA

Upstream 100 bases:

>100_bases
CTGCCTTCTTACTCTTTAATAGTAGCATTTTATTTTAAAAAAATAATTTGTTTTTTGGTAAAATTATAAATAACAATAAT
TATAGAAAGAGGTATCATAT

Downstream 100 bases:

>100_bases
TGGCTTTATAAGCCATTTTTTTAATATTAAACAAGGATAAAAGTTTGCTATTAAGCCCATAGAGAGTATAATAAATATAG
GAATATTTTTTATTTTTTTA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MPKKFYVTTPIYYPSGNLHIGHAYTTTLADILKRYKDMQGYETFFLTGSDEHGQKIEEKATEAGLTPIDYLEDKVESFKT
LWKALKIDYTKFIRTTDSYHEETVKKIFTLLLDKGFIYKGNYEGLYCISCEEFLNADQIDENNMCKISNTKLELVKEETY
FLKTSLFQEFMENEVLASEFLIPAYRRNEMLNSFVKPELKDLSVTRTSFSWGIPINEDPKHVVYVWLDALTNYITALGYL
QNDDSNFQKFWADENTEILQLAGKEIIRFHSIYWPIILKSLGLRQPTHLLGHGWILSKDTKMSKSLGNVIDPIEMINKYG
ADALRFYIGYELPTEKDGNFTDDLFIESFNAHLANNVGNLISRINQMITKYFDGFIENDNIVYDDNLTAKGIETINLYIQ
NMDQYKISDAIRNILDLGNACNKYIEETMPWNLAKEEKIDELKNVMATLQRNISIMVFLLKPILVENYEDMIEQIGLSGV
ELTFDKINNWDKIIFKKLGDKKIIFKRVN

Sequences:

>Translated_509_residues
MPKKFYVTTPIYYPSGNLHIGHAYTTTLADILKRYKDMQGYETFFLTGSDEHGQKIEEKATEAGLTPIDYLEDKVESFKT
L*KALKIDYTKFIRTTDSYHEETVKKIFTLLLDKGFIYKGNYEGLYCISCEEFLNADQIDENNMCKISNTKLELVKEETY
FLKTSLFQEFMENEVLASEFLIPAYRRNEMLNSFVKPELKDLSVTRTSFS*GIPINEDPKHVVYV*LDALTNYITALGYL
QNDDSNFQKF*ADENTEILQLAGKEIIRFHSIY*PIILKSLGLRQPTHLLGHG*ILSKDTKMSKSLGNVIDPIEMINKYG
ADALRFYIGYELPTEKDGNFTDDLFIESFNAHLANNVGNLISRINQMITKYFDGFIENDNIVYDDNLTAKGIETINLYIQ
NMDQYKISDAIRNILDLGNACNKYIEETMP*NLAKEEKIDELKNVMATLQRNISIMVFLLKPILVENYEDMIEQIGLSGV
ELTFDKINN*DKIIFKKLGDKKIIFKRVN
>Mature_508_residues
PKKFYVTTPIYYPSGNLHIGHAYTTTLADILKRYKDMQGYETFFLTGSDEHGQKIEEKATEAGLTPIDYLEDKVESFKTL
*KALKIDYTKFIRTTDSYHEETVKKIFTLLLDKGFIYKGNYEGLYCISCEEFLNADQIDENNMCKISNTKLELVKEETYF
LKTSLFQEFMENEVLASEFLIPAYRRNEMLNSFVKPELKDLSVTRTSFS*GIPINEDPKHVVYV*LDALTNYITALGYLQ
NDDSNFQKF*ADENTEILQLAGKEIIRFHSIY*PIILKSLGLRQPTHLLGHG*ILSKDTKMSKSLGNVIDPIEMINKYGA
DALRFYIGYELPTEKDGNFTDDLFIESFNAHLANNVGNLISRINQMITKYFDGFIENDNIVYDDNLTAKGIETINLYIQN
MDQYKISDAIRNILDLGNACNKYIEETMP*NLAKEEKIDELKNVMATLQRNISIMVFLLKPILVENYEDMIEQIGLSGVE
LTFDKINN*DKIIFKKLGDKKIIFKRVN

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=514, Percent_Identity=28.7937743190661, Blast_Score=218, Evalue=1e-56,
Organism=Homo sapiens, GI14043022, Length=500, Percent_Identity=26, Blast_Score=134, Evalue=3e-31,
Organism=Escherichia coli, GI1788432, Length=545, Percent_Identity=25.6880733944954, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1786861, Length=175, Percent_Identity=26.8571428571429, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI212645096, Length=519, Percent_Identity=29.4797687861272, Blast_Score=199, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI17540794, Length=554, Percent_Identity=25.4512635379061, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI71992781, Length=410, Percent_Identity=27.3170731707317, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI71997510, Length=171, Percent_Identity=29.2397660818713, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71997517, Length=171, Percent_Identity=29.2397660818713, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6321610, Length=544, Percent_Identity=30.8823529411765, Blast_Score=220, Evalue=3e-58,
Organism=Saccharomyces cerevisiae, GI6321704, Length=549, Percent_Identity=23.1329690346084, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21358483, Length=368, Percent_Identity=33.9673913043478, Blast_Score=197, Evalue=1e-50,
Organism=Drosophila melanogaster, GI24655311, Length=463, Percent_Identity=25.2699784017279, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI21355409, Length=166, Percent_Identity=28.3132530120482, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 57571; Mature: 57440

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKKFYVTTPIYYPSGNLHIGHAYTTTLADILKRYKDMQGYETFFLTGSDEHGQKIEEKA
CCCCEEEECCEEECCCCEEECHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHH
TEAGLTPIDYLEDKVESFKTLKALKIDYTKFIRTTDSYHEETVKKIFTLLLDKGFIYKGN
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEECC
YEGLYCISCEEFLNADQIDENNMCKISNTKLELVKEETYFLKTSLFQEFMENEVLASEFL
CCCEEEECHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IPAYRRNEMLNSFVKPELKDLSVTRTSFSGIPINEDPKHVVYVLDALTNYITALGYLQND
CCHHHHHHHHHHHCCCCHHHCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
DSNFQKFADENTEILQLAGKEIIRFHSIYPIILKSLGLRQPTHLLGHGILSKDTKMSKSL
CCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHCCCHHHHHHH
GNVIDPIEMINKYGADALRFYIGYELPTEKDGNFTDDLFIESFNAHLANNVGNLISRINQ
HHHCCHHHHHHHHCCHHEEHEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MITKYFDGFIENDNIVYDDNLTAKGIETINLYIQNMDQYKISDAIRNILDLGNACNKYIE
HHHHHHCCEECCCCEEECCCCCCCCHHEEEHEECCCCHHHHHHHHHHHHHHHHHHHHHHH
ETMPNLAKEEKIDELKNVMATLQRNISIMVFLLKPILVENYEDMIEQIGLSGVELTFDKI
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHCCCCEEEEEEEC
NNDKIIFKKLGDKKIIFKRVN
CCCEEEEEECCCCEEEEEECC
>Mature Secondary Structure 
PKKFYVTTPIYYPSGNLHIGHAYTTTLADILKRYKDMQGYETFFLTGSDEHGQKIEEKA
CCCEEEECCEEECCCCEEECHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHH
TEAGLTPIDYLEDKVESFKTLKALKIDYTKFIRTTDSYHEETVKKIFTLLLDKGFIYKGN
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEECC
YEGLYCISCEEFLNADQIDENNMCKISNTKLELVKEETYFLKTSLFQEFMENEVLASEFL
CCCEEEECHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IPAYRRNEMLNSFVKPELKDLSVTRTSFSGIPINEDPKHVVYVLDALTNYITALGYLQND
CCHHHHHHHHHHHCCCCHHHCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
DSNFQKFADENTEILQLAGKEIIRFHSIYPIILKSLGLRQPTHLLGHGILSKDTKMSKSL
CCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHCCCHHHHHHH
GNVIDPIEMINKYGADALRFYIGYELPTEKDGNFTDDLFIESFNAHLANNVGNLISRINQ
HHHCCHHHHHHHHCCHHEEHEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MITKYFDGFIENDNIVYDDNLTAKGIETINLYIQNMDQYKISDAIRNILDLGNACNKYIE
HHHHHHCCEECCCCEEECCCCCCCCHHEEEHEECCCCHHHHHHHHHHHHHHHHHHHHHHH
ETMPNLAKEEKIDELKNVMATLQRNISIMVFLLKPILVENYEDMIEQIGLSGVELTFDKI
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHCCCCEEEEEEEC
NNDKIIFKKLGDKKIIFKRVN
CCCEEEEEECCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1852609 [H]