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The map label for this gene is bglH [H]

Identifier: 50364849

GI number: 50364849

Start: 47227

End: 48678

Strand: Direct

Name: bglH [H]

Synonym: Mfl034

Alternate gene names: 50364849

Gene position: 47227-48678 (Clockwise)

Preceding gene: 50364848

Following gene: 50364851

Centisome position: 5.95

GC content: 27.13

Gene sequence:

>1452_bases
ATGAAATTTGAAAAAAAAGATTTTTTATGAGGAGGTGCAACTTCAGCTTCTCAAGTTGAAGGAGCTTTTGATGCGGAAGG
GAAATCATTAACAATTGCAGAAATGAGACCTTTCAATCCAAACCTAGATAGAAAAAGCGTAGATGAATTAAATAAATATA
CAAGATCTGATTATGAGAAATCAATTGATAATAAAGATAACCTTCATTATCCAAAAAGAATTGGCATTGATTTTTATCAC
AGATATAAAGAAGATATAGCTTTATTCAAAGAAGCAGGAATGAACATTTATAGAATGTCAATTGCTTGATCAAGAATATT
TCCTAATGGTGATGAAGCACAACCAAATGCAGCAGGTATTAAATTTTATAAAGATGTATTTGAAGAATGCAAAAAAAATG
GAATGGAAGTAATGTTAACTATTCAACATTATGATGTACCATATCCAATAACAAAAAAATATGGTGGTTGATCAAACAAA
AAAGTTATTGATTTATACATTAAATTTGCAACAGTTATTATGAAAGAATACAAAGACTTAGTTAAATATTGATTACCATT
CAATGAAATCAACGTAGCTACATTAGCTCCAACAACAGGTTTAGGTATTTTTAGAGAAGACTTTGATACTACTGAAGATT
ATAAAAATGCTTCATGACAAGGTTTACATAATCAATTTTATGCCCAAGCAAAAGTTATTGAGCTAGCAAAATCTATTTCA
AGCAATATTAAAATTGGGTGTATGGTTGCTAACATGACAACATACTCATCAGATTGTAACCCTGTAAATATTTGAGAAAA
TTTAGCTACTCAACAAATGCAAAGATATTTCTACTATGATGTAATGGTAAAAGGAGATTATCCATCATATTCAAAAAGAT
ATTTCAAAGAAAATAATATTAAATTTGAAACAACTCCTGAAGAAATGGAAGTAATTAAAAATAATAATATTCAATATATT
ACATTTAGTTACTATATGACATCAACAATTTCAAAAGAATTAAAAGATGCAGCAGGTGGTAATTTAATGATGGGTGGAAA
AAACCCATTCTTAAAAGCAACTGAATGGGGATGACAAATTGATCCTATTGGTTTAAGAATAACTTTAAATGAACTATGAG
ATAGATATCAATTGCCTTTATTCATTTCAGAAAATGGAATTGGTGTAATTGAAACTTTAAATGAAAATAACACAGTTGAT
GATGATTATAGAATAGAATATCTATCAAAACATTTTGAACAAATAAACGAAGCAATTAAAGATGGGGTTGATGTATTTGG
TTATACAATGTGAACTCCAATTGATGTTGTTAGTTTATCAACAAATGAAATGTCAAAAAGATATGGTTTAATATTTGTTG
ATTATGATGATTATCATAAAGGAACTGGAAATAGATTTAAAAAGAAATCATTTGAGTGATTCAAAAACTTTATAAAAACA
AAAGAATTATAA

Upstream 100 bases:

>100_bases
GCCTTAAACAGTGTTGAAATTGGATATGGTTTACAAGATAAAGTCGTTTATAAAATGCCAAAAGAATTTAAAGTAAAATT
AAAAAATAAAGAGGTGTAAT

Downstream 100 bases:

>100_bases
AAATAAAAAACTTTCTTAGGAAAGTTTTTTGTCTTAATATATTTTATTTTTCAACGGTGTTAATGTATAACAAATAATTG
GTATTGTTATCAAATATAAT

Product: 6-phospho-beta-glucosidase

Products: NA

Alternate protein names: 6-phospho-beta-glucosidase [H]

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MKFEKKDFLWGGATSASQVEGAFDAEGKSLTIAEMRPFNPNLDRKSVDELNKYTRSDYEKSIDNKDNLHYPKRIGIDFYH
RYKEDIALFKEAGMNIYRMSIAWSRIFPNGDEAQPNAAGIKFYKDVFEECKKNGMEVMLTIQHYDVPYPITKKYGGWSNK
KVIDLYIKFATVIMKEYKDLVKYWLPFNEINVATLAPTTGLGIFREDFDTTEDYKNASWQGLHNQFYAQAKVIELAKSIS
SNIKIGCMVANMTTYSSDCNPVNIWENLATQQMQRYFYYDVMVKGDYPSYSKRYFKENNIKFETTPEEMEVIKNNNIQYI
TFSYYMTSTISKELKDAAGGNLMMGGKNPFLKATEWGWQIDPIGLRITLNELWDRYQLPLFISENGIGVIETLNENNTVD
DDYRIEYLSKHFEQINEAIKDGVDVFGYTMWTPIDVVSLSTNEMSKRYGLIFVDYDDYHKGTGNRFKKKSFEWFKNFIKT
KEL

Sequences:

>Translated_483_residues
MKFEKKDFL*GGATSASQVEGAFDAEGKSLTIAEMRPFNPNLDRKSVDELNKYTRSDYEKSIDNKDNLHYPKRIGIDFYH
RYKEDIALFKEAGMNIYRMSIA*SRIFPNGDEAQPNAAGIKFYKDVFEECKKNGMEVMLTIQHYDVPYPITKKYGG*SNK
KVIDLYIKFATVIMKEYKDLVKY*LPFNEINVATLAPTTGLGIFREDFDTTEDYKNAS*QGLHNQFYAQAKVIELAKSIS
SNIKIGCMVANMTTYSSDCNPVNI*ENLATQQMQRYFYYDVMVKGDYPSYSKRYFKENNIKFETTPEEMEVIKNNNIQYI
TFSYYMTSTISKELKDAAGGNLMMGGKNPFLKATEWG*QIDPIGLRITLNEL*DRYQLPLFISENGIGVIETLNENNTVD
DDYRIEYLSKHFEQINEAIKDGVDVFGYTM*TPIDVVSLSTNEMSKRYGLIFVDYDDYHKGTGNRFKKKSFE*FKNFIKT
KEL
>Mature_483_residues
MKFEKKDFL*GGATSASQVEGAFDAEGKSLTIAEMRPFNPNLDRKSVDELNKYTRSDYEKSIDNKDNLHYPKRIGIDFYH
RYKEDIALFKEAGMNIYRMSIA*SRIFPNGDEAQPNAAGIKFYKDVFEECKKNGMEVMLTIQHYDVPYPITKKYGG*SNK
KVIDLYIKFATVIMKEYKDLVKY*LPFNEINVATLAPTTGLGIFREDFDTTEDYKNAS*QGLHNQFYAQAKVIELAKSIS
SNIKIGCMVANMTTYSSDCNPVNI*ENLATQQMQRYFYYDVMVKGDYPSYSKRYFKENNIKFETTPEEMEVIKNNNIQYI
TFSYYMTSTISKELKDAAGGNLMMGGKNPFLKATEWG*QIDPIGLRITLNEL*DRYQLPLFISENGIGVIETLNENNTVD
DDYRIEYLSKHFEQINEAIKDGVDVFGYTM*TPIDVVSLSTNEMSKRYGLIFVDYDDYHKGTGNRFKKKSFE*FKNFIKT
KEL

Specific function: Catalyzes the hydrolysis of aryl-phospho-beta-D- glucosides such as 4-methylumbelliferyl-phospho-beta-D- glucopyranoside (MUG-P), phosphoarbutin and phosphosalicin. Plays a major role in the utilization of arbutin or salicin as the sole carbon source. Bgl

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI32481206, Length=485, Percent_Identity=23.2989690721649, Blast_Score=113, Evalue=5e-25,
Organism=Homo sapiens, GI13273313, Length=478, Percent_Identity=24.8953974895398, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI110681710, Length=489, Percent_Identity=24.7443762781186, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI24497614, Length=455, Percent_Identity=25.2747252747253, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI28376633, Length=469, Percent_Identity=24.7334754797441, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI2367270, Length=483, Percent_Identity=41.8219461697723, Blast_Score=366, Evalue=1e-102,
Organism=Escherichia coli, GI1789070, Length=470, Percent_Identity=40.2127659574468, Blast_Score=357, Evalue=1e-99,
Organism=Escherichia coli, GI2367174, Length=476, Percent_Identity=38.4453781512605, Blast_Score=316, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI17552856, Length=496, Percent_Identity=26.6129032258064, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17539390, Length=492, Percent_Identity=26.8292682926829, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI21356577, Length=490, Percent_Identity=22.2448979591837, Blast_Score=99, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 54341; Mature: 54341

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFEKKDFLGGATSASQVEGAFDAEGKSLTIAEMRPFNPNLDRKSVDELNKYTRSDYEKS
CCCCCCCCCCCCCCHHHCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
IDNKDNLHYPKRIGIDFYHRYKEDIALFKEAGMNIYRMSIASRIFPNGDEAQPNAAGIKF
CCCCCCCCCCHHCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHCCCCCCCCCCCCHHHH
YKDVFEECKKNGMEVMLTIQHYDVPYPITKKYGGSNKKVIDLYIKFATVIMKEYKDLVKY
HHHHHHHHHHCCCEEEEEEEECCCCCCCHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHH
LPFNEINVATLAPTTGLGIFREDFDTTEDYKNASQGLHNQFYAQAKVIELAKSISSNIKI
CCCCCCEEEEECCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
GCMVANMTTYSSDCNPVNIENLATQQMQRYFYYDVMVKGDYPSYSKRYFKENNIKFETTP
EEEEEECEEECCCCCCCCHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHCCCEEEECCH
EEMEVIKNNNIQYITFSYYMTSTISKELKDAAGGNLMMGGKNPFLKATEWGQIDPIGLRI
HHHHHHHCCCEEEEEEEEHHHHHHHHHHHHHCCCCEEECCCCCCEECCCCCCCCCEEEEE
TLNELDRYQLPLFISENGIGVIETLNENNTVDDDYRIEYLSKHFEQINEAIKDGVDVFGY
ECCCCCCEECCEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHEEEE
TMTPIDVVSLSTNEMSKRYGLIFVDYDDYHKGTGNRFKKKSFEFKNFIKTKEL
EECCEEEEEECHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKFEKKDFLGGATSASQVEGAFDAEGKSLTIAEMRPFNPNLDRKSVDELNKYTRSDYEKS
CCCCCCCCCCCCCCHHHCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
IDNKDNLHYPKRIGIDFYHRYKEDIALFKEAGMNIYRMSIASRIFPNGDEAQPNAAGIKF
CCCCCCCCCCHHCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHCCCCCCCCCCCCHHHH
YKDVFEECKKNGMEVMLTIQHYDVPYPITKKYGGSNKKVIDLYIKFATVIMKEYKDLVKY
HHHHHHHHHHCCCEEEEEEEECCCCCCCHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHH
LPFNEINVATLAPTTGLGIFREDFDTTEDYKNASQGLHNQFYAQAKVIELAKSISSNIKI
CCCCCCEEEEECCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
GCMVANMTTYSSDCNPVNIENLATQQMQRYFYYDVMVKGDYPSYSKRYFKENNIKFETTP
EEEEEECEEECCCCCCCCHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHCCCEEEECCH
EEMEVIKNNNIQYITFSYYMTSTISKELKDAAGGNLMMGGKNPFLKATEWGQIDPIGLRI
HHHHHHHCCCEEEEEEEEHHHHHHHHHHHHHCCCCEEECCCCCCEECCCCCCCCCEEEEE
TLNELDRYQLPLFISENGIGVIETLNENNTVDDDYRIEYLSKHFEQINEAIKDGVDVFGY
ECCCCCCEECCEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHEEEE
TMTPIDVVSLSTNEMSKRYGLIFVDYDDYHKGTGNRFKKKSFEFKNFIKTKEL
EECCEEEEEECHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7883710; 7704263; 9384377 [H]